T4 RNA Ligase 1 (ssRNA Ligase) from New England Biolabs

Product Specs
ItemT4 RNA Ligase 1 (ssRNA Ligase)
CompanyNew England Biolabs
Price $71.00 Inquire
Catalog NumberM0204S
Concentration20 u/µl
Quantity1000 units
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit catalyzes the formation of 1nmol of [5`-32P]rA14-20 into a phosphatase resistant form in 30 minutes at 37°C in a total volume of 30µl.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Targeting RANKL-independent osteoclastogenesis overcomes denosumab resistance in models of ER+ breast cancer bone metastasisJ Clin InvestMay 15, 2026Qun Lin, Jinpeng Luo, Zhuxi Duan, Jieer Luo, Wei Zhang, Yuan Xia, Yinduo Zeng, Xiaolin Fang, Jiahui Liang, Jiayi Chen, et al.designed and prepared. Next, 50 μg linear RNAs was incubated overnight at 16°C with T4 RNA ligase 1 (NEB, M0204L) within a 500 μl reaction volume. After ethanol precipitation, the RNAs were separated on denaturing

(2) 3' UTR-mediated regulation of a protein chaperone by the pspA mRNA in Streptococcus pneumoniaeNucleic Acids ResMay 14, 2026Jens S Pettersen, Anette Lund, Finn Kirpekar, Nikolaj V Hansen, Sine L Svenningsen, James C Paton, Jakob Møller-Jensen, Mikkel G Jørgensencarried out to remove enzymes. RNA Adapter was ligated with T4 RNA ligase (20 U, New England BioLabs, M0204) overnight at 17°C. Reverse transcription was performed with the Maxima Reverse Transcriptase (200 U,

(3) Generation of precise and accurate engineered circRNAs using enzymatic ligationNucleic Acids ResMay 8, 2026Amrita Singh, Adela Dujsikova, Noah Mueller, Y Grace ChenMgCl2, and RNA ligase 2 buffer, and reactions were incubated at 25°C for 1 h. For RNA ligase 1 (NEB #M0204S) reactions, 25 units of the enzyme were added with 10% PEG, 1× RNA ligase buffer and incubated at 37°C

(4) Inherent Specificity and Variation Sensitivity as Quantitative Metrics for RBP BindingbioRxivMay 5, 2026Soon Yi, Shashi S. Singh, Xuan Ye, Rohan Krishna, Vidusha Kothwela, Eckhard Jankowsky, Joseph M. Lunasample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker.

(5) Conserved but mechanistically diverse piRNA defence against endogenous retroviruses in insectsEMBO ReportsApril 2, 2026Shashank Chary, Patricia E Carreira, Sarah Nicholas, Kathryn B McNamara, Ian A Cockburn, Karin Nordström, Therésa M Jones, Rosalyn Gloag, Alyson Ashe, Leon E Hugo, et al.,Thermo Fisher,EP0161 "T4 RNA Ligase 2, K227Q",New England Biolabs,M0373L T4 RNA Ligase 1,New England Biolabs,M0204S SuperScript II Reverse Transcriptase,Thermo Fisher,18064014 KAPA DNA polymerase,Sigma-Aldrich,KK3502 Software,Software,Software FASTX-toolkit,http://hannonlab.cshl.edu/fastx_toolkit/,N/A bowtie

(6) Engineering ligands for theophylline riboswitches expands its regulatory dynamic range in prokaryotic and eukaryotic systemsNature CommunicationsMarch 23, 2026Rushikesh M. Khadake, Krushna Shinde, Ambadas B. RodeNU-1706-CY5). The ligation reaction was performed in manufacturer-supplied buffer (New England Biolabs, M0204S) supplemented with 1 mM ATP, 20% (v/v) DMSO, and 0.6 U/μL T4 RNA ligase, incubated at 16 °C for 15 hours.

(7) A streamlined, nanopore-compatible 5PSeq protocol for rapid phenotypic antimicrobial sensitivity testingCell Rep MethodsMarch 23, 2026Honglian Liu, Susanne Huch, Ryan Hull, Fabricio Romero Garcia, Lilit Nersisyan, Xiushan Yin, Wei-Hua Chen, Juan Du, Vicent PelechanoAldrich,Cat# C0378 Erythromycin,Sigma Aldrich,Cat# E5389 T4 RNA ligase 1,New England Biolabs,Cat# M0204L SuperScript™ II Reverse Transcriptase,Thermo Fisher Scientific,Cat# 100004925 Phusion®High-Fidelity

(8) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie PylecDNAs (Supplementary Table S1), 0.5 mM ATP, 10 U T4 RNA ligase, 1X T4 RNA ligase buffer (NEB, cat #M0204S), and 25% PEG 8000. The reaction was incubated at 25°C for 16 hours, followed by enzyme deactivation

(9) TDP-43 dysfunction compromises UPF1-dependent mRNA metabolism in ALSNeuronFebruary 18, 2026Francesco Alessandrini, Matthew Wright, Tatsuaki Kurosaki, Olga S. Perloff, Marilyn Ngo, Julia K. Kofler, Christopher J. Donnelly, Lynne E. Maquat, Evangelos Kiskinis,New England Biolabs,M0530S T4 Polynucleotide Kinase,New England Biolabs,M0201S T4 RNA Ligase 1,New England Biolabs,M0204S Truncated T4 RNA Ligase 2,New England Biolabs,M0242S Recombinant Shrimp Alkaline Phosphatase,New England Biolabs

(10) Integrative profiling of condensation-prone RNAs during early developmentCell GenomicsFebruary 11, 2026Tajda Klobučar, Jona Novljan, Ira A. Iosub, Boštjan Kokot, Iztok Urbančič, D. Marc Jones, Anob M. Chakrabarti, Nicholas M. Luscombe, Jernej Ule, Miha Modicperformed as in the original protocol. Proximity ligation was performed using a different T4 RNA ligase (M0204L, NEB) and hence 1 mM ATP was added to the overnight reaction. Finally, cells were lysed using 200 μL

(11) Systematic Identification of Germ Granule Proteins Reveals Specialized Roles in RNAi and Small RNA InheritancebioRxivFebruary 1, 2026Shihui Chen, Lucas H. Prescott, Craig C. Mello, Carolyn M. Phillipsthen hybridized to the reverse transcription primer, ligated to the 5’ adapter with T4 RNA ligase (NEB M0204L), and reverse transcribed with Superscript III (Thermo Fisher 18080–051). Small RNA libraries were amplified

(12) In vivo AGO-APP for cell-type- and compartment-specific miRNA profiling in the mouse brainCell Reports MethodsJanuary 26, 2026Surbhi Kapoor, Andrea Erni, Francesca Vincenzi, Beatrice Tessier, Vasika Venugopal, Gunter Meister, Alexandre Favereaux, Harold Cremer, Christophe BeclinFirst Strand cDNA Super Mix,Thermo Fisher Scientific,18080–400 T4 RNA Ligase 1,New England Biolabs,M0204S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, ChromoTek

(13) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. GraveleyμL of the prepared ligation mixture was combined with 3 μL of T4 RNA Ligase 1 (New England Biolabs, #M0204S) and 2 μL of the 5’-RNA linker (50 μM), bringing the final reaction volume to 40 μL. The reaction was

(14) Multiple Quality Control Checkpoints Safeguard Small Nuclear RNA Biogenesis and Prevent Assembly of Aberrant SpliceosomesbioRxivJanuary 16, 2026Tiantai Ma, Claire Huntington, Zhuoyi Song, Rea M. Lardelli, Eric L. Van Nostrand, Jens Lykke-AndersenT4 Polynucleotide Kinase,NEB,Cat #P8107S FastAP Alkaline Phosphatase,ThermoFisher,Cat #EF0651 T4 RNA Ligase 1,NEB,Cat #M0204L "T4 RNA Ligase 1 (ssRNA Ligase), High Concentration",NEB,Cat #M0437M RNaseOUT™ Recombinant Ribonuclease Inhibitor

(15) ZMYND11 Restrains KMT2A to Enable a Neuronal Developmental ProgrambioRxivJanuary 16, 2026Alexander W. Greben, Xiaoli S. Wu, Josephine E. Robb, Ian R. Vogel, Lisa Traunmüller, Sebastian Krüttner, Kara A. Fulton, Migue Van Louis Darcera, Eric C. Griffith, Michael E. GreenbergSuperscript III Reverse Transcriptase,Invitrogen,Cat# 18080044 T4 Polynucleotide Kinase,NEB,Cat# M0201 T4 RNA Ligase 1,NEB,Cat# M0204 Other Reagents,, BioMag Plus Concanavalin A Beads,Bangs,Cat# BP531 Eukitt Quick-Hardening Mounting Medium

(16) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al."RNase Inhibitor, Murine",New England Biolabs,Cat#M0314L T4 RNA Ligase 1 (ssRNA Ligase),New England Biolabs,Cat#M0204L T4 DNA ligase,New England Biolabs,Cat#M0202 Amylose Resin,New England Biolabs,Cat#E0821S HiScribe® T7 High Yield RNA Synthesis Kit

(17) The DinG exonuclease acts as a primary quality controller to remove unprocessed ribosomal RNAsNucleic Acids ResearchJanuary 6, 2026Karolis Vaitkevičius, Jörgen JohanssonFor 3′ end labeling the RNA was ligated to [32P]pCp (Hartmann Analytic) using T4 RNA ligase 1 (NEB, M0204L) and cleaned-up using Oligo Clean & Concentrator kit (Zymo Research). The RNA oligonucleotide pre23S_ends_5xPTO

(18) Genome-wide profiling of RNA 2’- O -methylation in neurons and identification of orphan snoRNA targetsbioRxivJanuary 1, 2026Xuan Ye, Yaling Liu, Yinzhou Zhu, Sarah A. Alshawi, Brittany A. Elliott, Christopher L. Holley, Jean-Denis Beaudoin, Brenton R. Graveley, Gordon G. CarmichaelμL of the prepared ligation mixture was combined with 3 μL of T4 RNA Ligase 1 (New England Biolabs, #M0204S) and 2 μL of the 5’-RNA linker (50 μM), bringing the final reaction volume to 40 μL. The reaction was

(19) RG motifs promote piRNA-mediated gene silencing in C. elegansNucleic Acids ResDecember 11, 2025Dylan C Wallis, Carolyn M Phillipsthen hybridized to the reverse transcription primer, ligated to the 5′ adapter with T4 RNA ligase (NEB M0204L), and reverse-transcribed with SuperScript III (Thermo Fisher, 18080-051). Small RNA libraries were

(20) CTCF couples long-range loop extrusion and diffusion to mediate a diverse Igκ repertoireNature CommunicationsDecember 11, 2025Emma L. Bush, Brigette Berke-Reynolds, Kaitlyn M. Hutchins, Xinrui Yu, Jorge A. Colón-Rosado, Fujung Chang, John Curran, Jiaxin Yang, Liangliang Sun, Jianrong Wang, et al.M0201S), and sequentially ligated to 5’ and 3’ RNA adaptors by T4 RNA ligase (New England Biolabs, M0204S) with Streptavidin bead enrichments after each ligation. Reverse transcription was performed using Maxima

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