CELLSTAR® Filter Cap Cell Culture Flasks from Greiner Bio-One GmbH

Product Specs
ItemCELLSTAR® Filter Cap Cell Culture Flasks
CompanyGreiner Bio-One GmbH
Price Get Pricing Get Quote for this Product
Catalog Number658175 / 660175 / 661175 / 690175
ApplicationsCell culture

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Greiner Bio-One GmbH

Maybachstraße 2

72636 Frickenhausen

Germany

Phone: +49 7022 948-0
Fax: +49 7022 948-514

Email: info@de.gbo.com

Company Profile

Website: www.gbo.com

(1) Highly specific σ2R/TMEM97 ligand FEM-1689 alleviates neuropathic pain and inhibits the integrated stress responseProc Natl Acad Sci U S ADecember 26, 2023Muhammad Saad Yousuf, James J. Sahn, Hongfen Yang, Eric T. David, Stephanie Shiers, Marisol Mancilla Moreno, Jonathan Iketem, Danielle M. Royer, Chelsea D. Garcia, Jennifer Zhang, et al.medium (10% FBS, 1% Pen-Strep in DMEM/F12 + GlutaMax (Gibco #10565-018) in T-75 flasks (Greiner Bio-One #658175). In a 96-well plate (ThermoFisher #165305), cells were plated at a density of 20,000 cells per well

(2) CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cellsiScienceDecember 15, 2023Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, et al.BSC-II (biosafety cabinets type-II). TC-treated vessels used for iPSC cultures: 25cm2 (T25; Greiner #690175) and 75cm2 flasks (T75; Greiner #658175) with filter lid, 96-well plates (Corning #3595), 24-well (Corning

(3) Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophagesSTAR ProtocolsDecember 1, 2023Ignazio Antignano, Lily Keane, Melania Capassonot TC-treated sterile Petri dish,Corning,Cat#351058 75 cm2 cell culture flask,Greiner Bio-One,Cat#658175 96-well clear bottom black TC-treated sterile microplate,Corning,Cat#3603 96-well clear V-bottom sterile

(4) A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiationSTAR ProtocolsDecember 1, 2023Bianca Parisi, Maxime Sünnen, Rohan Chippalkatti, Daniel Kwaku Abankwacounter,Beckman Coulter,Cat# 9914591 Cuvette Coulter,VWR,Cat# 720-0812 T75 flask,Greiner Bio-One,Cat# 658175 Eppendorf 1.5 mL,Greiner Bio-One,Cat# 616201 Falcon 15 mL,Greiner Bio-One,Cat# 188271 Falcon 50 mL,Greiner Bio-One

(5) Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screensSTAR ProtocolsDecember 1, 2023Oscar Bril, Laura J. Schwarzmueller, Leandro F. Moreno, Louis Vermeulen, Nicolas Léveilléal.8,https://bioconductor.org/packages/release/bioc/html/EnhancedVolcano.html ,, Other,Other,Other ,, Culture flask 50 mL 25 cm2,Greiner Bio-One,Cat#690175 Culture flask 250 mL 75 cm2,Greiner Bio-One,Cat#658175 CELLSTAR 6 well culture plates,Greiner Bio-One,Cat#657160 "CELLSTAR

(6) Immune monitoring of SARS-CoV-2-specific T cell and B cell responses in patients with multiple sclerosis treated with ocrelizumabFrontiers in ImmunologySeptember 20, 2023Elina Groß-Albenhausen, Alicia Weier, Markus Velten, Thorsten Heider, Rittika Chunder, Stefanie Kuertenadded for polyclonal B cell stimulation. Cells were then incubated in cell culture flasks (Greiner, #690175) at 37°C and 7% CO2 for 6 days. On day 5, 96-well ELISPOT plates (Merck, #MSIPN4W50) were coated as

(7) Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell linesSTAR ProtocolsSeptember 1, 2023Mawien Karaca, Kristin Fritsche, Dajana Lichtenstein, Özlem Vural, Katrin Kreuzer, Jimmy Alarcan, Albert Braeuning, Philip Marx-Stoelting, Tewes TralaumL)",VWR, "Cell culture flasks and plates, sterile (25 cm2, 75 cm2, 6-well, 96-well)",Greiner,"Cat. nos. 690175, 658175, 657160, and 655180" "Dark 2 mL reaction tubes, e.g., Eppendorf safe-lock tubes, ambra (light

(8) Protocol to optimize the biobanking of ovarian cancer organoids by accommodating patient-specific differences in stemness potentialSTAR ProtocolsSeptember 1, 2023Fabian Trillsch, Bastian Czogalla, Fabian Kraus, Alexander Burges, Sven Mahner, Mirjana KesslerTC-treated,Corning,3548 Falcon® 24-well Polystyrene,Corning,351447 "CELLSTAR cell culture flask, 75 cm2",Greiner Bio-one,658175 "CELLSTAR cell culture flask, 25 cm2",Greiner Bio-one,690160 "CELLSTAR cell culture flask, 175 cm2",Greiner

(9) Image-based and machine learning-guided multiplexed serology test for SARS-CoV-2Cell Reports MethodsAugust 22, 2023Vilja Pietiäinen, Minttu Polso, Ede Migh, Christian Guckelsberger, Maria Harmati, Akos Diosdi, Laura Turunen, Antti Hassinen, Swapnil Potdar, Annika Koponen, et al.paper,https://doi.org/10.5281/zenodo.8158791 ,, Other,Other,Other ,, T-75 cell culture flask,"CELLSTAR®, Greiner Bio-one",CAT#658175 Cellcarrier ultra (PE) 384 imaging plates,PerkinElmer,CAT#6057302 T-75 cell culture flask,Corning,CAT#430641U "384

(10) Pharmacological blockade of cannabinoid receptor 2 signaling does not affect LPS/IFN-γ-induced microglial activationScientific ReportsJuly 10, 2023Bolanle Fatimat Olabiyi, Anne-Caroline Schmoele, Eva Carolina Beins, Andreas Zimmerbovine serum (PAA, cat# A15-108) in a poly-L-Lysine coated T75 culture flask (Greiner bio-one, cat# 658175). The medium was changed twice a week until confluency was reached (approximately 14 days). Microglia

(11) Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brainLife science allianceJuly 1, 2023Stroo E, Janssen L, Sin O, Hogewerf W, Koster M, Harkema L, Youssef SA, Beschorner N, Wolters AH, Bakker B, et al.isolated from Serf2+/+, Serf2+/−, and Serf2−/− E13.5 embryos. MEFs were cultured in T75 culture flasks (658175; Greiner Bio-One), high-glucose DMEM (Gibco), supplemented with 10% FBS (12133C; Sigma-Aldrich), 1%

(12) Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulantsRegenerative TherapyJune 1, 2023Rasmus Roost Aabling, Toke Alstrup, Emma Mader Kjær, Kristine Juul Poulsen, Jonas Oute Pedersen, Anne Louise Revenfeld, Bjarne Kuno Møller, Marco EijkenCLS431751). This stromal vascular fraction (SVF) was seeded in T175 culture flasks (Greiner Bio-One-Gr-660175) and incubated at 37 °C in humid air with 5% CO2. Next day, culture medium was exchanged twice to remove

(13) Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregatesSTAR ProtocolsJune 1, 2023Piyumi Wijesekara, Kian Z. Patel, Ellen L. Otto, Phil G. Campbell, Xi Ren124-0045 "Wide-orifice pipette tips, 200 μL",Axygen,T-205-WB-C-R-S 75-cm2 cell culture flask,Greiner Bio-One,Cat# 658175 175-cm2 cell culture flask,Greiner Bio-One,Cat# 660175 "96-well cell-repellent, U-bottom microplate",Greiner

(14) Two Methods for the Isolation and Cultivation of Porcine Primary Corneal CellsMethods and ProtocolsMay 12, 2023Alice Rocha Teixeira Netto, Marc Dieter Hrusa, Karl-Ulrich Bartz-Schmidt, Sven Schnichels, José Hurst10103578001).Culture flask with ventilation screw cap T25 (Greiner Bio-One Gmbh, Frickenhausen, Germany; Cat. No. 690175).Disposable cup 100 mL (Sarstedt, Nümbrecht, Germany; Cat. No. 75.563).Disposable safety scalpel #11

(15) Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway OrganoidsBio-protocolApril 20, 2023Chiu MC, Li C, Yu Y, Liu X, Huang J, Wan Z, Yuen KY, Zhou J.culture dish (Corning, catalog number: 430167)T175 cell culture flask (Greiner Bio-One, catalog number: 661175)Nunc non-treated 24-well plate (Thermo Scientific, catalog number: 144530)Nunclon Sphera 24-well plate

(16) Validation of σ 2 R/TMEM97 as a neuropathic pain target: Specificity, human expression and mechanism of actionbioRxivApril 18, 2023Muhammad Saad Yousuf, James J. Sahn, Eric T. David, Stephanie Shiers, Danielle M. Royer, Chelsea D. Garcia, Jennifer Zhang, Veronica M. Hong, Ayesha Ahmad, Benedict J. Kolber, et al.medium (10% FBS, 1% Pen-Strep in DMEM/F12 + GlutaMax (Gibco #10565–018) in T-75 flasks (Greiner Bio-One #658175). Cells were maintained at 37 °C with 5% CO2 and passaged twice before being plated onto either 96-well

(17) The Cultivation Modality and Barrier Maturity Modulate the Toxicity of Industrial Zinc Oxide and Titanium Dioxide Nanoparticles on Nasal, Buccal, Bronchial, and Alveolar Mucosa Cell-Derived Barrier ModelsInternational Journal of Molecular SciencesMarch 15, 2023Helene Stuetz, Eva I. Reihs, Winfried Neuhaus, Maren Pflüger, Harald Hundsberger, Peter Ertl, Christian Resch, Gerald Bauer, Günter Povoden, Mario Rothbauerhumidified 37 °C, 5% CO2, in TC-treated 75 cm2 flasks (Greiner Bio-One, Kremsmünster, Austria, Cat. Nr. 658175) and split twice a week by trypsinization (0.5 g/L Trypsin–0.2 g/L EDTA, Sigma-Aldrich, Vienna, Austria,

(18) One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporationSTAR ProtocolsMarch 1, 2023Donovan Flumens, Diana Campillo-Davo, Ibo Janssens, Gils Roex, Jorrit De Waele, Sébastien Anguille, Eva LionCoulter,https://www.beckman.pt/flow-cytometry/research-flow-cytometers/cytoflex/software ,, Other,Other,Other ,, CELLSTAR® T75 Flask culture flask,Greiner Bio-One,Cat# 658175 CELLSTAR® 15 mL Falcon tubes,Greiner Bio-One,Cat# 188271 CELLSTAR® 50 mL Falcon tubes,Greiner Bio-One,Cat#

(19) Discovery of Targets for Immune–Metabolic Antitumor Drugs Identifies Estrogen-Related Receptor AlphaCancer DiscoveryMarch 1, 2023Avinash Sahu, Xiaoman Wang, Phillip Munson, Jan P.G. Klomp, Xiaoqing Wang, Shengqing Stan Gu, Ya Han, Gege Qian, Phillip Nicol, Zexian Zeng, et al.Procedures siRNA Treatment of Cells. SK-BR-3 cells were seeded in a T75 flask (Greiner Bio-One, #658175) and transfected 4 days later with 25 nmol/L siRNA-ESRRA (Dharmacon; #L-03403) using dharmafect 1 (Dharmacon,

(20) In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cellsSTAR ProtocolsMarch 1, 2023Sanne van der Heijden, Donovan Flumens, Maarten Versteven, Stefanie Peeters, Hans De Reu, Diana Campillo-Davo, Yannick Willemen, Benson Ogunjimi, Viggo Van Tendeloo, Zwi N. Berneman, et al.188271 CELLSTAR® 50 mL Falcon tubes,Greiner Bio-One,Cat# 227261 CELLSTAR® T175 culture flask,Greiner Bio-One,Cat# 660175 CELLSTAR® 24-well plate,Greiner Bio-One,Cat# 662160 2 mL cryovials,Sarstedt,Cat# 72-694-006 ABX Micros

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(1)

10/23/2023Leak Proof Cell Culture Flask From Greiner Danielle SchmidtGreat for cell culture. The holes provide gas exchange without allowing contamination. Has a good seal and the tissue culture bottom allows uniform growth. T175 is large enough to hold ~20E6 cells ... Read Review
(1)

7/15/2022High Quality Cell Culture Flasks Daniel HamiltonI used these cell culture flask with many different cell lines. I have never had any problems. I usually seed my cells with 12ml of medium.