SignalFire ™ ECL Reagent from Cell Signaling Technology

Product Specs
ItemSignalFire ™ ECL Reagent
CompanyCell Signaling Technology
Price $344.00 Inquire
Catalog Number6883S
Quantity500 ml
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Protocol for isolation of the brain vasculature from the mouse cortex for proteomicsSTAR ProtocJuly 21, 2026Yazi Huang, Veronica Clementel, Mingzi Zhang, Kate Martinez, Karen Martinez, Gavin Spillard, Carina Torres-Sepulveda, Kassandra Kisler, Marcelo P. Coba, Ruslan Rustblocker (nonfat dry milk),Bio-Rad,Cat# 1706404 SignalFire ECL reagent,Cell Signaling Technology,Cat# 6883 Ketamine HCl (100 mg/mL),MWI Veterinary Supply Co.,N/A Xylazine HCl (20 mg/mL),MWI Veterinary Supply

(2) Lactate-fueled H3K18 lactylation sustains ΔNp63α-dependent self-renewal in limbal epithelial stem cellsJournal of Biological ChemistryJuly 1, 2026Longfei Li, Lin Zhu, Liyuan Wang, Jie Kang, Mingyue Zheng, Ziqian Geng, Tao Yu, Bin Xufor 30 min at room temperature. Protein bands were visualized using ECL Western Blotting Substrate (6883S, Cell Signaling Technology) and captured using a Tanon 5200 Chemiluminescent Imaging System. Band intensity

(3) Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in MelanomaAdvanced ScienceMay 26, 2026Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, et al.additional TBST washes, protein bands were visualized using ECL Substrate (Cell Signaling Technology, 6883S) and imaged with a Tanon 5200Multi Imaging System. Primary antibodies used were as follows: phospho‐MEK

(4) An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cellsbioRxivMay 26, 2026Takuya Kamikawa, Cole J. Wilson, Ivy Lan, Yuta Nihongakiat room temperature. Bands were detected using SignalFire™ ECL Reagent (Cell Signaling Technology, 6883) and imaged on ChemiDoc MP system (Bio-Rad). Band sizes were compared against Precision Plus Protein Kaleidoscope Prestained Protein Standards

(5) Extracellular vesicles from SB431542-conditioned human adipose-derived stem cells enhance in vitro and in vivo osteogenesis via a miR-20a-5p-BAMBI-BMP/canonical Wnt signaling axisStem Cells Transl MedMay 24, 2026Julika Huber, Michael T Longaker, Natalina QuartoCell Signaling) was used. Immunoblotted proteins were visualized by enhanced chemiluminescence (ECL) (#6883, Cell Signaling). Images of immune-positive bands were captured by a ChemiDoc Imaging System (Bio-Rad).

(6) Nitric oxide drives proteomic diversity through alternative splicingMol CellMay 21, 2026Joseph C. Schindler, Puneet Seth, Alfred Hausladen, E. Ricky Chan, Jun Yang, Jun Qin, Zhaoxia Qian, Divya Seth, Kathleen Lundberg, Joseph M. Luna, et al.Buffer,BioRad,Cat#1610737 2-mercaptoethanol,Sigma-Aldrich,Cat#M6250 Blotting grade blocker non-fat dry milk,BioRad,Cat#1706404XTU SignalFire ECL Reagent,Cell Signaling,Cat#6883 TRIzol Reagent,Invitrogen,Cat#15596026 GelRed Nucleic Acid Stain,Millipore,Cat#SCT123 DNase I,New England Biolabs

(7) Lactate modulates microglial M2 polarization via H3K9 lactylation in ischemic strokeJournal of Translational Internal MedicineApril 28, 2026Bingwei Li, Kan Xu, Jinlu Yuantibodies (Proteintech, RGAR001/SA00001; 1:5000) were applied for 1 h prior to detection using ECL (CST, 6883). Quantification of the resulting signals was performed using Image Lab software (Bio-Rad). Cut& Tag

(8) Regulatory BC200 RNA promotes breast carcinogenesis by repressing BRCA1 gene expressioniScienceApril 17, 2026Valerio Berardi, Ilham A. Muslimov, Dan Sun, Giuseppe Militello, Prem Premsrirut, Henri TiedgeTriton X-100,Sigma-Aldrich,T8787 Nitrocellulose membranes,BioRad,– SignalFire ECL Reagent,Cell Signaling Technology,6883 Western Blot Stripping Buffer,Thermo Fischer Scientific,PI21059 TRIzol Reagent,Invitrogen,10-296-010 Superscript

(9) Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling PathwayDose ResponseApril 7, 2026Huahui Bian, Dongyang Zhao, Haoyu Wang, Hao Huang, Youyou Wang, Weibo Chen, Chang Liu, Yinyin Yang, Anqing Wu, Yulong Liuvisualized using an enhanced chemiluminescence (ECL) detection kit (Cell Signaling Technology, Cat. No. 6883), and recorded utilizing a polychromatic fluorescence chemiluminescence imaging analysis system (FluorChem

(10) Expanding the Global Map of Protein Post Translational Modifications with Immunoaffinity Enrichment and nDIA Analysis on the Orbitrap Astral Mass SpectrometerMolecular & Cellular ProteomicsApril 6, 2026Mukesh Kumar, Anthony P. Possemato, Barry M. Zee, Srikanth Subramanian, Jian Min Ren, Alissa J. Nelson, Bin Zhang, Sean D. Landry, Jeffrey C. Silva, Brett Larsen, et al.washes, proteins were detected using enhanced chemiluminescence reagent SignalFire ECL Reagent (CST #6883) and visualized with a ChemiDoc imaging system (Bio-Rad). Cell/Tissue Lysis, Protein Extraction and

(11) Synergistic effects through targeting the PI3K and IGFR pathways in treating lung cancer carrying activation alterations along the PI3K pathwayTransl OncolApril 3, 2026Mohamed Abd El-Salam, Wu Chen, Yan Tang, Ting Rao, Xuejia Kang, Lifang Sun, Tiegang Han, Pengyu Chen, Matthew Mossanen, Fan Cheng, et al.EveryBlot Blocking Buffer (#12010020, Bio-Rad). Immunoreactive bands were detected using SignalFire™ ECL Reagent (#6883, Cell Signaling Technology) and SuperSignal™ West Femto Maximum Sensitivity Substrate (#34094, Thermo Scientific

(12) Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formationNature CommunicationsMarch 30, 2026Yongtao Du, Xilin Miao, Yu Gao, Yu Liang, Xue Bai, Song Dang, Zhongsheng Wu, Zhengyang An, Xinyi Zhang, Yaran Yang, et al.at room temperature. The membranes were incubated with the SignalFireTM ECL Reagent (Cell Signaling, 6883S) or BeyoECL Moon (Beyotime, P0018FS) and imaged using the Tanon-5200 chemiluminescent imaging system

(13) UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytesFrontiers in ImmunologyMarch 19, 2026Riss M. Kell, Margaret K. Weber, Rosa Y. Escalante, Jeffrey C. Silva, Andrea G. Bodnarwashed three times with 1x TBST. HRP signal was visualized using SignalFire (Cell Signaling Technology, #6883). Membranes were imaged using a UVP ChemStudio imaging system (Analytik Jena). Western blot images (8

(14) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.additional washes with TBST, membranes were developed using ECL Substrate (Cell Signaling Technology, 6883S) and visualized with a Protein Imaging System (Tanon, 5200Multi). Enzyme-linked immunosorbent assay ELISAs

(15) Methyl Gallate Enhances Post-Thaw Boar Sperm Quality by Alleviating Oxidative Stress and Preserving Mitochondrial FunctionAntioxidants (Basel)December 7, 2025Yonghui Bu, Deming Shi, Jiahao Li, Xiaoxiang Jiang, Yuhan Chen, Zhenjun Wu, Wanxin Li, Li Li, Shouquan Zhang, Hengxi WeiCST, Danvers, MA, USA) for 1 h at 27 °C. Protein bands were visualized using an ECL detection kit (6883S, CST, Danvers, MA, USA) and imaged with a Tanon imaging system (Tanon, Shanghai, China). Band intensities

(16) CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progressionCell Death DiscovOctober 9, 2025Yumei Gong, Marc Leon, Huaqing Mo, Premkamol Pengpaeng, Hai Yang, Yanxi Lu, Zhiqiang Yin, Alan Benard, Yong Zhou, Robert Grützmann, et al.antibodies. Signals were developed using Signal Fire TM ECL Reagent (Cell Signaling Technology Cat# 6883S) and imaged using the Amersham Imager 600 system (Pittsburgh, USA). The intensities of bands were analyzed

(17) αB-crystallin enhances radioresistance in basal-like breast cancer cells by suppressing caspase-3 activationSci ProgMay 20, 2025Yan Yuan, Xubaihe Zhang, Ying Xu, Yangyang Ge, Anqing Wutemperature for 1 hour. Signals were detected using an ECL (enhanced chemiluminescence) kit (Cat. No: 6883; Cell Signaling Technology, USA) and recorded utilizing a polychromatic fluorescence chemiluminescence

(18) CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal CancerCancers (Basel)May 12, 2025Feng Xue, Hai Yang, Pengyan Xu, Shuman Zhang, Nathalie Britzen-Laurent, Li-Li Bao, Robert Grützmann, Christian Krautz, Christian PilarskySignalFire Elite ECL Reagent (Cell Signaling Technology Cat#12757S) or SignalFire ECL Reagent (Cell Signaling Technology Cat#6883S). Finally, the intensity of the Western blot band was quantified using ImageJ (RRID:SCR_003070). 5.9.

(19) Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing lossNeural Regeneration ResearchMay 1, 2025Xihang Chen, Zhifeng Chen, Menghua Li, Weiwei Guo, Shuolong Yuan, Liangwei Xu, Chang Lin, Xi Shi, Wei Chen, Shiming Yangtemperature. Finally, the bands were visualized using an enhanced chemiluminescence detection kit (6883S, Cell Signaling Technology), and protein expression levels were assessed and recorded using GraphPad

(20) Head and neck squamous cell carcinoma-derived extracellular vesicles mediate Ca²⁺-dependent platelet activation and aggregation through tissue factorCell Commun SignalMay 1, 2025Tobias Weiser, Cosima C. Hoch, Julie Petry, Maria Shoykhet, Benedikt Schmidl, Mina Yazdi, Khouloud Hachani, Julia Mergner, Marie-Nicole Theodoraki, Omid Azimzadeh, et al.#7076, Cell Signaling Technology). Signals were developed using enhanced chemiluminescence substrate (#6883, Cell Signaling Technology) and visualized with the ChemiDoc XRS + system (Bio-Rad). Image analysis

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10/12/2020Great ECL Substrate Sachin SamuchiwalI use this reagent to develop my Western blots. Here I am showing the blot of Beta actin developed by SignalFire ECL reagent.