SignalFire ™ ECL Reagent from Cell Signaling Technology

Product Specs
ItemSignalFire ™ ECL Reagent
CompanyCell Signaling Technology
Price $344.00 Inquire
Catalog Number6883S
Quantity500 ml
ApplicationsW
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Lactate modulates microglial M2 polarization via H3K9 lactylation in ischemic strokeJournal of Translational Internal MedicineApril 28, 2026Bingwei Li, Kan Xu, Jinlu Yuantibodies (Proteintech, RGAR001/SA00001; 1:5000) were applied for 1 h prior to detection using ECL (CST, 6883). Quantification of the resulting signals was performed using Image Lab software (Bio-Rad). Cut& Tag

(2) Regulatory BC200 RNA promotes breast carcinogenesis by repressing BRCA1 gene expressioniScienceApril 17, 2026Valerio Berardi, Ilham A. Muslimov, Dan Sun, Giuseppe Militello, Prem Premsrirut, Henri TiedgeTriton X-100,Sigma-Aldrich,T8787 Nitrocellulose membranes,BioRad,– SignalFire ECL Reagent,Cell Signaling Technology,6883 Western Blot Stripping Buffer,Thermo Fischer Scientific,PI21059 TRIzol Reagent,Invitrogen,10-296-010 Superscript

(3) Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling PathwayDose ResponseApril 7, 2026Huahui Bian, Dongyang Zhao, Haoyu Wang, Hao Huang, Youyou Wang, Weibo Chen, Chang Liu, Yinyin Yang, Anqing Wu, Yulong Liuvisualized using an enhanced chemiluminescence (ECL) detection kit (Cell Signaling Technology, Cat. No. 6883), and recorded utilizing a polychromatic fluorescence chemiluminescence imaging analysis system (FluorChem

(4) Synergistic effects through targeting the PI3K and IGFR pathways in treating lung cancer carrying activation alterations along the PI3K pathwayTransl OncolApril 3, 2026Mohamed Abd El-Salam, Wu Chen, Yan Tang, Ting Rao, Xuejia Kang, Lifang Sun, Tiegang Han, Pengyu Chen, Matthew Mossanen, Fan Cheng, et al.EveryBlot Blocking Buffer (#12010020, Bio-Rad). Immunoreactive bands were detected using SignalFire™ ECL Reagent (#6883, Cell Signaling Technology) and SuperSignal™ West Femto Maximum Sensitivity Substrate (#34094, Thermo Scientific

(5) UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytesFrontiers in ImmunologyMarch 19, 2026Riss M. Kell, Margaret K. Weber, Rosa Y. Escalante, Jeffrey C. Silva, Andrea G. Bodnarwashed three times with 1x TBST. HRP signal was visualized using SignalFire (Cell Signaling Technology, #6883). Membranes were imaged using a UVP ChemStudio imaging system (Analytik Jena). Western blot images (8

(6) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.additional washes with TBST, membranes were developed using ECL Substrate (Cell Signaling Technology, 6883S) and visualized with a Protein Imaging System (Tanon, 5200Multi). Enzyme-linked immunosorbent assay ELISAs

(7) Methyl Gallate Enhances Post-Thaw Boar Sperm Quality by Alleviating Oxidative Stress and Preserving Mitochondrial FunctionAntioxidants (Basel)December 7, 2025Yonghui Bu, Deming Shi, Jiahao Li, Xiaoxiang Jiang, Yuhan Chen, Zhenjun Wu, Wanxin Li, Li Li, Shouquan Zhang, Hengxi WeiCST, Danvers, MA, USA) for 1 h at 27 °C. Protein bands were visualized using an ECL detection kit (6883S, CST, Danvers, MA, USA) and imaged with a Tanon imaging system (Tanon, Shanghai, China). Band intensities

(8) CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progressionCell Death DiscovOctober 9, 2025Yumei Gong, Marc Leon, Huaqing Mo, Premkamol Pengpaeng, Hai Yang, Yanxi Lu, Zhiqiang Yin, Alan Benard, Yong Zhou, Robert Grützmann, et al.antibodies. Signals were developed using Signal Fire TM ECL Reagent (Cell Signaling Technology Cat# 6883S) and imaged using the Amersham Imager 600 system (Pittsburgh, USA). The intensities of bands were analyzed

(9) Muscle afferent ASIC3 upregulation mediates the exacerbated exercise pressor reflex in male rats following hindlimb ischemia-reperfusionPhysiological reportsJuly 1, 2025Qin L, Zhang X, Li J.Immunoreactivity was visualized using an enhanced chemiluminescence system (Cell Signaling Technology, Cat no.: 6883). The membrane was stripped and incubated with an anti‐β‐actin primary antibody (1:1000, Sigma Aldrich,

(10) αB-crystallin enhances radioresistance in basal-like breast cancer cells by suppressing caspase-3 activationSci ProgMay 20, 2025Yan Yuan, Xubaihe Zhang, Ying Xu, Yangyang Ge, Anqing Wutemperature for 1 hour. Signals were detected using an ECL (enhanced chemiluminescence) kit (Cat. No: 6883; Cell Signaling Technology, USA) and recorded utilizing a polychromatic fluorescence chemiluminescence

(11) CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal CancerCancers (Basel)May 12, 2025Feng Xue, Hai Yang, Pengyan Xu, Shuman Zhang, Nathalie Britzen-Laurent, Li-Li Bao, Robert Grützmann, Christian Krautz, Christian PilarskySignalFire Elite ECL Reagent (Cell Signaling Technology Cat#12757S) or SignalFire ECL Reagent (Cell Signaling Technology Cat#6883S). Finally, the intensity of the Western blot band was quantified using ImageJ (RRID:SCR_003070). 5.9.

(12) Head and neck squamous cell carcinoma-derived extracellular vesicles mediate Ca²⁺-dependent platelet activation and aggregation through tissue factorCell Commun SignalMay 1, 2025Tobias Weiser, Cosima C. Hoch, Julie Petry, Maria Shoykhet, Benedikt Schmidl, Mina Yazdi, Khouloud Hachani, Julia Mergner, Marie-Nicole Theodoraki, Omid Azimzadeh, et al.#7076, Cell Signaling Technology). Signals were developed using enhanced chemiluminescence substrate (#6883, Cell Signaling Technology) and visualized with the ChemiDoc XRS + system (Bio-Rad). Image analysis

(13) Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing lossNeural Regeneration ResearchMay 1, 2025Xihang Chen, Zhifeng Chen, Menghua Li, Weiwei Guo, Shuolong Yuan, Liangwei Xu, Chang Lin, Xi Shi, Wei Chen, Shiming Yangtemperature. Finally, the bands were visualized using an enhanced chemiluminescence detection kit (6883S, Cell Signaling Technology), and protein expression levels were assessed and recorded using GraphPad

(14) Inhibiting glycolysis facilitated checkpoint blockade therapy for triple-negative breast cancerDiscov OncolApril 17, 2025Chong Li, Yu Tang, Ruizhi Zhang, Liang Shi, Jianying Chen, Peng Zhang, Ning Zhang, Wei LiProteintech, 1:3000) for 1 h at room temperature. Then the membrane was visualized using ECL reagents (#6883, Cell Signaling Technology) with the Invitrogen iBright CL1000 imaging system (Thermo Fisher Scientific). Quantitative

(15) IGF1 Signaling Regulates Neuropeptide Expression in Hypothalamic Neurons Under Physiological and Pathological ConditionsEndocrinologyMarch 24, 2025Wenyuan He, Neruja Loganathan, Denise D Belshamto imaging, which was performed using the SignalFire ECL Reagent (Cell Signaling Technology, Inc., 6883) on an iBright™ FL1500 or iBright™ FCL1500 imager. Alpha-tubulin was imaged on the same blot using the

(16) Use of antibodies against Epstein–Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformationArchives of VirologyDecember 1, 2024Christian Graesser, Ruth Nord, Heinrich Flaswinkel, Elisabeth Kremmer, Eckart Meese, Karolina Magdalena Caban, Thomas Fröhlich, Friedrich A. Grässer, Martin Hart1 mM MgCl2, and 0.5% IGEPAL, and analyzed by immunoblotting and enhanced chemiluminescence (ECL) (#6883, Cell Signaling Technology, Danvers, Massachusetts, USA) detection [1]. For release experiments with

(17) Interleukin-33 mediated regulation of microRNAs in human cord blood-derived mast cells: Implications for infection, immunity, and inflammationPLOS ONENovember 26, 2024Sherin Bakhashab, Ghalya H. Banafea, Farid Ahmed, Nadia Bagatian, Ohoud Subhi, Hans-Juergen Schulten, Peter Natesan Pushparajwith 10 ng of IL-33 for 24 h. Among these genes, 57 were upregulated in response to a decrease in miR-6883 expression (Fig 2). The top three physiological functions (p < 0.05) were hematological system development

(18) Inhibition of miR-20a-5p Suppresses Epithelial-Mesenchymal Transition of Colorectal Cancer Cells Through GJA1Molecular BiotechnologyNovember 22, 2024Lu Zhang, Jun-Bin Wang, Zhen-Yuan Gao, Xiao Wu, Hai-Rong Zhouperoxidase-conjugated anti-rabbit or anti-mouse IgG (SA00001-2 or SA00001-1, Proteintech). Ultimately, ECL reagent (6883, CST) was adopted to visualize the protein bands and ImageJ software was utilized to analyze the bands. Immunohistochemistry Obtained

(19) Colchicine prevents perioperative myocardial injury in cardiac surgery by inhibiting the formation of neutrophil extracellular traps: evidence from rat modelsEuropean Journal of Cardio-Thoracic SurgeryOctober 7, 2024Hao-Dong Pan, You-Ru Kong, Li Xu, Ming-Yue Liu, Zhi-Kang Lv, Yusanjan Matniyaz, Hai-Tao Zhang, Yu-Xian Tang, Wen-Xin Su, Chen-Yu Jiang, et al.peroxidase-conjugated secondary antibody (ab6721, Abcam, USA), and protein bands were visualized using an ECL kit (#6883, Cell Signaling Technology, USA). Detailed methodology is provided in Supplementary Material, Methods

(20) V1bR enhances glucose-stimulated insulin secretion by paracrine production of glucagon which activates GLP-1 receptorCell & BioscienceAugust 31, 2024Ying Yun, Shimeng Guo, Xin Xietemperature. Immunostaining was visualized using Signal Fire™ ECL Reagent (Cell Signaling Technology, #6883) and images were taken with a ChemiDocXRS imaging system (Bio-Rad). Oral glucose tolerance test and

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10/12/2020Great ECL Substrate Sachin SamuchiwalI use this reagent to develop my Western blots. Here I am showing the blot of Beta actin developed by SignalFire ECL reagent.