GoScript Reverse Transcription System from Promega

Product Specs
ItemGoScript Reverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5001
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolinPoult SciApril 9, 2026Yiming Zhang, Zhe Li, Xinyu Liu, Bing Zhao, Tong Xuthe TRIzol extraction method, and first-strand cDNA was synthesized using a qRT-PCR synthesis kit (A5001, Promega, Beijing, China) according to the manufacturer's instructions (Lu et al., 2022). Gene expression

(2) NOP58 modulates radiosensitivity in non-small cell lung cancer via DDX18-mediated DNA damage repairJ Radiat ResMarch 25, 2026Yiqian Jiang, Weijun Zhang, Yanhong Bao, Fang Su, Bo Wang(2 μg) was subsequently reverse transcribed into cDNA using GoScript™ Reverse Transcription System (A5001, Promega, USA). PCR amplification system was formulated according to the instructions of TB Green® Premix

(3) A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3Noncoding RNA ResMarch 19, 2026Xiaoyang Cao, Juzheng Sui, Ge Shan, Xu LiucDNA synthesis. Reverse transcription was performed using the GoScript Reverse Transcription System (Promega, A5001) according to the manufacturer's instructions, with random hexamer primers. PCR was performed using

(4) Multi-omics analysis identifies CCNB1 as a cell cycle factor driving glioblastoma progression and its inhibition by resveratrolPLoS OneMarch 16, 2026Bohan Liu, Dazhao Peng, Yankun Chen, Qiuling Li, Yuedong Hu, Shiyu Liu, Huimin HuTRIzol reagent (Invitrogen). The RNA was then reverse transcribed into cDNA using a reverse transcription kit (A5001, Promega, USA). The qPCR reaction mixture was prepared, typically consisting of 10 μL of PowerUp™ SYBR™

(5) Bone from Healthy Individuals and Patients with CKD Expresses the Sodium-Glucose Co-transporter-2 (SGLT2)Calcif Tissue IntMarch 13, 2026Lauter Eston Pelepenko, Luciene Machado Dos Reis, Luzia Naoko Shinohara Furukawa, Rodrigo Bueno de OliveiraWaltham, MA, USA). Transcription to cDNA was performed using GoScript™ reverse transcription system (Cat A5001, Promega, USA) according to the manufacturer’s instructions, consistently using 1 µg of the total RNA.

(6) Asiatic Acid-Loaded Hydrogel Ameliorates Diabetic Periodontitis via Mitophagy-Mediated M2 Macrophage PolarizationACS OmegaFebruary 24, 2026Yuhan Yang, Yanlin Zhu, Hongyan Yuan, Zun Yang, Jie Zhouspectrophotometer (Thermo Fisher, USA). cDNA was synthesized using GoScript Reverse Transcription System (A5001, Promega, USA). qPCR reactions contained cDNA mixed with SYBR Green (A6001, Promega, USA) and 10 μM

(7) Epigenetic landscape of the H3K27me3 mark in macrophages transformed by Theileria annulataCommunications BiologyFebruary 24, 2026Takaya Sakura, Shahin Tajeri, Zineb Rchiad, Hifzur R. Ansari, Abhinav Kaushik, Tobias Mourier, Arnab Pain, Michel Wassef, Gordon Langsleywas done by using 1 µg RNA as template and the GoScript™ reverse transcriptase kit (Promega, Cat. No. A5001, USA), in 20 µl reaction volume, following the manufacturer’s instructions. To perform qRT-PCR, target

(8) Single-nucleus multi-omics analysis of mouse small-intestinal Lgr5+ cell populations reveals Foxa3-induced Paneth cell-lineage differentiationCommunications BiologyFebruary 21, 2026Xinran Deng, Shenfei Sun, Chenqi Lu, Hanxiao Du, Xiaoyu You, Fujing Huang, Kai Gong, Jingxin Guo, Yumou Gong, Runrong Li, et al.74034). Reverse transcription was conducted using the GoScript™ Reverse Transcription kit (Promega, A5001). RT-PCR was performed, and relative mRNA levels were normalized to the Gapdh mRNA levels. The RT-PCR

(9) Downmodulation of cholesterol biosynthetic network governs activation of the innate immune response to Japanese encephalitis virus infectionJournal of VirologyFebruary 4, 2026Sakshi Khera, Kiran Bala Sharma, Yashwant Kumar, Manjula Kaliasynthesized from the extracted RNA using random hexamers and the GoScript Reverse Transcriptase kit (A5001, Promega). qRT-PCR reactions were set up on the QuantStudio 6 (Applied Biosystems) using SYBR Premix

(10) Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinomaMolecular cancerFebruary 4, 2026Wang JY, Lu YQ, Tan XR, Ma SS, Dai JH, Feng SY, Duan YF, Bai JW, Li YQ, Gong S, et al.(Invitrogen, 15596018), and cDNA was synthesized with the GoScript Reverse Transcription System (Promega, A5001). Genomic DNA from bacterial strains and NPC cells was extracted using the TIANamp Bacteria DNA Kit

(11) Threonic acid, an ascorbic acid metabolite, synergizes with intermittent fasting to ameliorate obesityExperimental & molecular medicineFebruary 1, 2026Oh S, Park S, Kim EK.Fisher Scientific). cDNA was synthesized from 3 µg of RNA using GoScript Reverse Transcriptase (Promega, A5001). mRNA levels were quantified by quantitative real-time PCR using TB Green (SYBR) Premix Ex Taq II (TaKaRa,

(12) Cashew Nut Oil Improves Lipid Metabolism and Fat Liver Deposition in High‐Fat Diet‐Fed C57BL / 6J MiceLipidsJanuary 1, 2026Marcella Duarte Villas Mishima, Andressa Ladeira Bernardes, Carlos Alexandre Pinheiro, Luisa Gouveia Lana, Iasmim Xisto Campos, Valéria Silva de Lana, Izabela Maria Montezano de Carvalho, Patrícia Fontes Pinheiro, Renata Celi Lopes Toledo, Ana Paula Dionísio, et al.washing with 75% ethanol. To synthesize the cDNA, the GoScript Reverse Transcription System (Promega, A5001, USA) was used, following the manufacturer's instructions. 2.8 Determination of Gene Expression of

(13) The Distinct Monocyte Subsets and Intercellular Communication in Primary Sjögren's Syndrome Revealed by Single-Cell RNA SequencingJournal of Clinical ImmunologyDecember 27, 2025Xiaoyu Zhang, Xiangrong Song, Li Fang, Bo Zang, Jiaxiu Li, Yifei Yang, Meng Xu, Yuanwei Han, QiXuan Liu, Hong Zhu, et al.Qualified RNA samples were reverse transcribed using the GoScript Reverse Transcription System (Cat# A5001, Promega) in a 20 μL reaction volume containing 2 μg of RNA input, with incubation at 42 °C for 60 minutes

(14) Advancing leprosy risk prediction through identification of a whole blood host transcriptomic biomarker signature including non-coding genesScientific ReportsDecember 27, 2025Matheus Rogerio Almeida, Elisa Gherardi, Suzanne van Veen, Luca Maharavo, Aart Rosmolen, Emma van Eerde, Fons J. Verbeek, Marufa Khatun, Santosh Soren, Abu Sufian Chowdhury, et al.deactivation phase at 70 °C for 15 min using the GoScript™ Reverse Transcription System kit (Promega A5001) following the manufacturer’s instructions. Subsequently, a quantitative PCR was performed by mixing

(15) Exosomal miR-3126-5p derived from cancer-associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axisClin Transl MedDecember 18, 2025Zhenyu Zhang, Haicheng Ma, Yingying Zheng, Lina Wang, Chenghui Wang, Yuanyuan Liu, Hengxiao Lu, Shaoqiang WangFisher Scientific), then reverse‐transcribed to cDNA using the GoScript Reverse Transcription System (A5001, Promega). Then, qPCR was conducted on the 7500 Real‐Time PCR system (Applied Biosystems, Foster City,

(16) Vitamin B6 promotes the activation of primordial follicles through the PI3K/Akt signaling pathwayJ Ovarian ResDecember 17, 2025Jianzhong Wen, Wenqian Li, Zhijuan Wang, Luchun Zhang, Yating Huang, Mengjie Weng, Ye Chen, Zuyao Hou, Meijia Zhang, Yuezhou Chentotal RNA from each group was reverse-transcribed using the GoScript™ Reverse Transcription System (A5001, Promega, Madison, WI, USA). Data normalization was performed using ribosomal protein L19 (Rpl19). The

(17) Therapeutic potential of targeting ceramide for early cardiometabolic lipotoxicity in vivo studyScientific ReportsDecember 14, 2025Youssef M. Shalaby, Bashar Al-Zohily, Manjusha Sudhadev, Abderrahim Nemmar, Suhail Al-Salam, Nadia Akawiand 18 S rRNA bands. cDNA synthesis was performed with the GoScript™ Reverse Transcriptase Kit (Cat# A5001, Promega, USA). Gene expression was quantified using SYBR™ Green Universal Master Mix (Cat# 4364344,

(18) Tppp3 determines basal body positioning and identity of respiratory cilia via microtubule assembly and sphingolipid homeostasisProc Natl Acad Sci U S ADecember 9, 2025Takafumi Sakai, Masahiro Kawakita, Arashi Seki, Katsuyoshi Takaoka, Misaki Kurata, Junpei Torikai, Sohshiroh Atsumi, Kanji Yawata, Kentaro Noi, Yosuke Fukutani, et al.311-0736). Complementary DNA (cDNA) was prepared using GoScript™ Reverse Transcriptase kit (Promega, A5001). Quantitative PCR was conducted with cDNA diluted to 10 ng/µL using Fast SYBR Green qPCR Master Mix

(19) Verbascoside targets endothelial HIF-1α/ Lysyl oxidase signaling to attenuate glomerular injury in diabetic nephropathyRedox RepDecember 8, 2025Tianyu Kang, Bin Hou, Min Shi, Huan Liu, Yanan Li, Kaixin Li, Shuxin Li, Zetong Wu, Zhaopeng Xu, Mengnan Li(cDNA) synthesis from the RNA template subsequently employed a reverse transcription kit (Promega, Madison, USA, A5001). Using the resulting cDNA as template and GAPDH primers for internal control, polymerase chain reaction (PCR

(20) Rbfox2 selectively governs hematopoietic stem cell self-renewal by regulating proteostasisSci AdvDecember 5, 2025Longfei Gao, Desmond Dickson, Huijuan Feng, Chaolin Zhang, Lei DingRNA was subjected to reverse transcription with GoScript Reverse Transcriptase (Promega, catalog no. A5001) following the manufacturer’s instructions. qPCR was performed using GoTaq qPCR Master Mix (Promega,

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