ddPCR™ 96-Well Plates from Bio-Rad

Product Specs
ItemddPCR™ 96-Well Plates
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number12001925
Quantity1
FormatSemi-skirted
VolumeMaximum well volume: 250 µl
Brand/TrademarkLSG DBG

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Potent latency reversal by Tat RNA-containing nanoparticle enables multi-omic analysis of the HIV-1 reservoirNat CommunDecember 18, 2023Marion Pardons, Basiel Cole, Laurens Lambrechts, Willem van Snippenberg, Sofie Rutsaert, Ytse Noppe, Nele De Langhe, Annemieke Dhondt, Jerel Vega, Filmon Eyassu, et al.sorted on a BD FACSAria Fusion Cell Sorter. Cells were sorted in skirted 96-well PCR plates (Biorad, #12001925). To avoid evaporation during the sort, the PCR plate was continuously chilled at 4 °C. For STIP-Seq

(2) In vivo genome editing using 244-cis LNPs and low-dose AAV achieves therapeutic threshold in hemophilia A miceMolecular Therapy - Nucleic AcidsDecember 12, 2023Jeong Pil Han, Yeji Lee, Jeong Hyeon Lee, Hye Yoon Chung, Geon Seong Lee, Yu Ri Nam, Myeongjin Choi, Kyoung-Sik Moon, Haeshin Lee, Hyukjin Lee, et al.When droplet generation was completed, the droplets were transferred to a ddPCR 96-well PCR plate (12001925, Bio-Rad) using a multichannel pipet. The plate was heat-scaled with foil and placed in a T100 Thermal Cycler

(3) Arginine vasopressin injection rescues delayed oviposition in cyp19a1b -/- mutant female zebrafishFront Endocrinol (Lausanne)December 8, 2023Katherine Shaw, Chunyu Lu, Xiaochun Liu, Vance L. Trudeauemulsified samples were then carefully transferred to the wells of a 96-well ddPCR plate (Bio-Rad, cat# 12001925) and sealed with a heat foil (Bio-Rad, cat# 1814040). The PCR reactions were performed using a thermal

(4) Extracellular vesicle-derived CircWhsc1 promotes cardiomyocyte proliferation and heart repair by activating TRIM59/STAT3/Cyclin B2 pathwayJournal of advanced researchNovember 1, 2023Wei G, Li C, Jia X, Xie J, Tang Z, Jin M, Chen Q, Sun Y, He S, Li X, et al.QX200 droplet generator (#1864002, Bio-Rad). The resulting droplets were transferred to a 96-well plate (#12001925, Bio-Rad), and the plate was heat-sealed with foil followed by PCR. The cycling protocol was as follows:

(5) A rapid, multiplex digital PCR assay to detect gene variants and fusions in non‐small cell lung cancerMolecular OncologyNovember 1, 2023Bryan Leatham, Katie McNall, Hari K. K. Subramanian, Lucien Jacky, John Alvarado, Dominic Yurk, Mimi Wang, Donald C. Green, Gregory J. Tsongalis, Aditya Rajagopal, et al.10977015; Invitrogen, Waltham, MA, USA). Each ddPCR reaction mix was added to a 96‐well PCR plate (PN 12001925; Bio‐Rad), sealed with a PX1 PCR Plate Sealer (Bio‐Rad), then vortexed three times for 10 s pulses,

(6) Molecular tuning of sea anemone stingingeLifeOctober 31, 2023Lily S He, Yujia Qi, Corey A H Allard, Wendy A Valencia-Montoya, Stephanie P Krueger, Keiko Weir, Agnese Seminara, Nicholas W Bellono(QX200 Droplet Generator BioRad Cat #1864002) and transfer of droplets to ddPCR 96-Well Plates (Bio-Rad Cat #12001925) were performed according to manufacturer’s instructions (Instruction Manual, QX200 Droplet Generator

(7) Development of Seven New dPCR Animal Species Assays and a Reference Material to Support Quantitative Ratio Measurements of Food and Feed ProductsFoodsOctober 20, 2023Kate R. Griffiths, Jacob L. H. McLaughlin, Felicity Hall, Lina Partis, Samuel C. Hansen, Rachel Tulloch, Daniel G. Burkeof 25 µL. Reaction mixes were transferred to 96-well semi-skirted plates (Bio-Rad, catalogue number 12001925), sealed with a foil heat-seal and centrifuged for 30 s before being placed in the AutoDG (Bio-Rad),

(8) Adenovirus protein VII binds the A-box of HMGB1 to repress interferon responsesPLOS PathogensSeptember 13, 2023Edward A. Arnold, Robin J. Kaai, Katie Leung, Mia R. Brinkley, Laurel E. Kelnhofer-Millevolte, Monica S. Guo, Daphne C. Avgoustigenerated (QX200 Droplet Generator). Generated droplets were transferred to a 96-well PCR plate (Bio-Rad 12001925), and PCR amplification was performed with a Bio-Rad C100 Touch Thermal Cycler under the following conditions:

(9) HSV-1 exploits host heterochromatin for nuclear egressJournal of Cell BiologySeptember 4, 2023Hannah C. Lewis, Laurel E. Kelnhofer-Millevolte, Mia R. Brinkley, Hannah E. Arbach, Edward A. Arnold, Saskia Sanders, Jens B. Bosse, Srinivas Ramachandran, Daphne C. Avgoustiplaced in the droplet generator (Bio-Rad). Droplets generated were transferred to a 96-well PCR plate (12001925; Bio-Rad), and PCR amplification was performed on a Bio-Rad C1000 Touch Thermal Cycler with the following

(10) Molecular tuning of sea anemone stingingbioRxivAugust 3, 2023Lily S He, Yujia Qi, Corey AH Allard, Wendy A Valencia-Montoya, Stephanie P Krueger, Keiko Weir, Agnese Seminara, Nicholas W Bellono(QX200™ Droplet Generator BioRad Cat #1864002) and transfer of droplets to ddPCR™ 96-Well Plates (Bio-Rad Cat #12001925) were performed according to manufacturer’s instructions (Instruction Manual, QX200™ Droplet Generator

(11) Bacterial DNA on the skin surface overrepresents the viable skin microbiomeeLifeJune 30, 2023Ellen M Acosta, Katherine A Little, Benjamin P Bratton, Jaime G Lopez, Xuming Mao, Aimee S Payne, Mohamed Donia, Danelle Devenport, Zemer GitaiSupplementary file 1. Reaction mixtures were transferred to sterile ddPCR 96-well plates (Bio-Rad #12001925) which were loaded into the QX200 Automated Droplet Generator. After droplet generation, the plate was

(12) ACE-2, TMPRSS2, and Neuropilin-1 Receptor Expression on Human Brain Astrocytes and Pericytes and SARS-CoV-2 Infection KineticsInternational Journal of Molecular SciencesMay 11, 2023Johid Reza Malik, Arpan Acharya, Sean N. Avedissian, Siddappa N. Byrareddy, Courtney V. Fletcher, Anthony T. Podany, Shetty Ravi Dyavartested in four replicates (n=4) in the ddPCR assay in a 96 well plate (Biorad, Hercules, CA, USA # 12001925) and were sealed with Biorad Sealer PX1, and droplets were generated in AutoDG instrument (Biorad, Hercules,

(13) Genetic profile of thymic epithelial tumors in the Japanese population: an exploratory study examining potential therapeutic targetsTranslational Lung Cancer ResearchApril 28, 2023Midori Shimada, Hirokazu Taniguchi, Hiroyuki Yamaguchi, Hiroshi Gyotoku, Daisuke Sasaki, Norihito Kaku, Chikako Senju, Hiroaki Senju, Erika Imamura, Shinnosuke Takemoto, et al.generation, the droplets were carefully transferred to a twin-tec, semi-skirted, 96-well PCR plate (Catalog #12001925, Bio-Rad), after which the plate was sealed two times for 5 s at 180 °C using a PX1 PCR Plate Sealer.

(14) A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in salivaScientific ReportsFebruary 22, 2023Kaitlyn Wagner, Phil Fox, Elizabeth Gordon, Westen Hahn, Kenzie Olsen, Alex Markham, Dylan Buglewicz, Platon Selemenakis, Avery Lessard, Daniella Goldstein, et al.and 127.5 nM, respectively. RT-ddPCR reactions were set up in 96-well ddPCR plates (BioRad, cat. No. 12001925) referred to as PCR plates (PP). Plates were covered with a pierceable foil heat seal (BioRad, cat.

(15) Several cell-intrinsic effectors drive type I interferon-mediated restriction of HIV-1 in primary CD4 + T cellsbioRxivFebruary 12, 2023Hannah L. Itell, Daryl Humes, Julie Overbaugh(ddPCR) amplification to measure vector copies per cell. Per well of a 96-well ddPCR plate (Bio-Rad, #12001925), 3 μL of diluted template DNA was used as input, along with 1.8 μL of each 10 μM vector-specific primer

(16) Visualizing Cathepsin K‐Cre Expression at the Single‐Cell Level with GFP ReportersJBMR PlusJanuary 1, 2023Wenhuan Chai, Weiwei Hao, Jintao Liu, Zhenglin Han, Shiyu Chang, Liben Cheng, Mingxin Sun, Guofang Yan, Zemin Liu, Yin Liu, et al.were transferred from the top wells of the cartridge into a PCR plate (ddPCR TM96‐Well Plates, Bio‐Rad‐12001925) and sealed with the PX1TM PCR Plate Sealer. Then the plate was placed in a PCR thermal cycler to amplify

(17) Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNAHuman GenomicsDecember 31, 2022Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, et al.QX100 Droplet Generator. Droplets were then transferred from the cartridge to a 96-well plate (Bio-Rad, 12001925) and a thermocycling program was performed on a C1000 Touch Thermal cycler (Bio-Rad): 95 °C for 10 min,

(18) Protocol for high-throughput reservoir quantification across global HIV subtypes using a cross-subtype intact proviral DNA assaySTAR ProtocolsDecember 1, 2022Carolyn S. Fish, Noah A.J. Cassidy, Claire N. Levy, Sean M. Hughes, Keith R. Jerome, Julie Overbaugh, Florian Hladik, Dara A. LehmanmL),N/A,N/A Nanodrop 2000 Spectrophotometer,Thermo Scientific,Cat## ND-2000-US-CAN ddPCR 96-well plates,Bio-Rad,Cat# 12001925 DG8 gasket,Bio-Rad,Cat# 1863009 DG8 Cartridge holder,Bio-Rad,Cat# 1863051 DG8 Droplet generation

(19) Beta2-adrenoreceptor agonist clenbuterol produces transient decreases in alpha-synuclein mRNA but no long-term reduction in proteinnpj Parkinson's DiseaseDecember 1, 2022Joseph R. Patterson et al.From the droplet well of the cartridge, 40 µL of droplets are transferred to a 96-well plate (Bio-Rad, 12001925). When all samples are transferred, the plate was sealed with pierceable foil (Bio-Rad, 181-4040) by

(20) Genetic variation at mouse and human ribosomal DNA influences associated epigenetic statesGenome BiologyDecember 1, 2022Francisco Rodriguez-Algarra et al.sample well. The ddPCR reactions were prepared in duplicate in a ddPCR™ 96-well plate (Bio-Rad, Cat. 12001925). The components of the reaction mix per reaction were 11 μl iTaq Universal Probe Supermix (Bio-Rad,

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