Corning® Spheroid Microplate from Corning Life Sciences

Product Specs
ItemCorning® Spheroid Microplate
CompanyCorning Life Sciences
Price Supplier Page View Company Product Page
Catalog NumberSpheroid Microplate
Applications3D Cell Culture
QuantityEA

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Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Rhenium(I) Tricarbonyl Complexes of 1,10-Phenanthroline Derivatives with Unexpectedly High CytotoxicityInorganic ChemistryAugust 7, 2023Lucy E. Enslin, Kallol Purkait, Maria Dalla Pozza, Bruno Saubamea, Pierre Mesdom, Hendrik G. Visser, Gilles Gasser, Marietjie Schutte-SmithSpheroid 1 × 104 PC3 cells were seeded in each well of a Corning 96 Well Ultra-Low Attachment Treated Spheroid Microplate. After 96 h of incubation at 37 °C in normoxic conditions, the average spheroid size observed was 435

(2) Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell deatheLifeDecember 5, 2022Borges JP, Sætra RSR, Volchuk A, Bugge M, Devant P, Sporsheim B, Kilburn BR, Evavold CL, Kagan JC, Goldenberg NM, et al.(R&D Systems, 293-VE-050). Cell suspension, supplemented with 10 μM Y-27632, was added into a 384-well Spheroid Microplate (Corning. #3830), 50 µL/well. The microplate was centrifuged at 1000 RPM for 3 min, and incubated at

(3) Engineering consortia by polymeric microbial swarmbotsNature CommunicationsDecember 1, 2022Lin Wang et al.Synergy H1) with the excitation at 580 nm and the emission at 610 nm using a 384-well plate (Corning Spheroid Microplate). Consortia assembly by free cells BY4742(S. cerevisiae) constitutively expressing eGFP or MG1655

(4) YAP Activation in Promoting Negative Durotaxis and Acral Melanoma ProgressionCellsNovember 9, 2022Yuxing Huang, Jing Su, Jiayong Liu, Xin Yi, Fang Zhou, Jiaran Zhang, Jiaxiang Wang, Xuan Meng, Lu Si, Congying Wu5000 cells (100μL resuspended solution) were added per well in a 96-well Corning Ultra-Low Attachment Spheroid Microplate (Corning) then incubated for 48 h. 2 mg/mL collagen was coated on the bottom of a 12-well plate in a

(5) Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilitiesCell GenomicsSeptember 14, 2022Roberta Esposito, Taisia Polidori, Dominik F. Meise, Carlos Pulido-Quetglas, Panagiotis Chouvardas, Stefan Forster, Paulina Schaerer, Andrea Kobel, Juliette Schlatter, Erik Kerkhof, et al.are detached, counted, and seeded onto 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate (Corning #4520) in 20 μL domes of Matrigel Matrix GFR, LDEV-free (Corning #356231) and RPMI-1640 growth

(6) Shipping Temperature, Time and Media Effects on Equine Wharton’s Jelly and Adipose Tissue Derived Mesenchymal Stromal Cells CharacteristicsAnimals (Basel)August 3, 2022Eleonora Iacono, Aliai Lanci, Penelope Gugole, Barbara Merlotissues. Cells were cultured in a multiwell Corning 96-well Black/Clear Round Bottom Ultra- Low Attachment Spheroid Microplate (5000 cells/25 μL drop). Images were acquired after 24 and 48 hrs of culture by a CCD camera (DS-Fi2,

(7) Photosubstitution in a trisheteroleptic ruthenium complex inhibits conjunctival melanoma growth in a zebrafish orthotopic xenograft model†Chemical ScienceJune 15, 2022Quanchi Chen et al.(Gibco® Life Technologies, cat# 11058021) were seeded in the low-attachment 96 well plates (Corning Spheroid microplate 4515) and incubated in normoxia (21% O2). After 24 h, 100 μL per well of diluted [2](PF6)2 with six

(8) Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cellsRegenerative therapyMarch 1, 2022Ichikawa A, Neo S, Nukui R, Yamada Y, Nitta S, Iwaki H, Yanagi Y, Nakayama K, Sato S, Tateishi S, et al.cell mixtures were seeded in 96-well plates (96-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate; Corning, New York, USA) and prepared with HGM, EGM-2, and MSCGM in a ratio of HGM: EGM-2: MSCGM = 3:

(9) Mineralizing Gelatin Microparticles as Cell Carrier and Drug Delivery System for siRNA for Bone Tissue EngineeringPharmaceuticsFebruary 28, 2022Hinkelmann S, Springwald AH, Schulze S, Hempel U, Mitrach F, Wölk C, Hacker MC, Schulz-Siegmund M.of cGM suspension (0.64 mg ml−1) in Opti-MEM™ with 10% FBS per well of a low adhesion 96-well plate (Spheroid microplate, Corning, NY, USA). After 24 h, the medium was changed to osteogenic medium (OM) based on DMEM, 10%

(10) Microtissues from mesenchymal stem cells and siRNA-loaded cross-linked gelatin microparticles for bone regenerationMaterials Today BioJanuary 1, 2022Sandra Hinkelmann, Alexandra H. Springwald, Annett Starke, Hermann Kalwa, Christian WÃ¶lk, Michael C. Hacker, Michaela Schulz-Siegmundor 8.3 mg•ml−1) in OptiMEM® supplemented with 10% FBS in one well of a low adhesion 96 well plate (Spheroid microplate, Corning, NY, USA) and gently shaken for 1–3 s. Based on the calculated surface area of cGM (calculated

(11) Microfluidic-Assisted Preparation of Targeted pH-Responsive Polymeric Micelles Improves Gemcitabine Effectiveness in PDAC: In Vitro InsightsCancers (Basel)December 21, 2021Rosa Maria Iacobazzi et al.PANC−1 tumor homo-spheroids formation, cells were seeded at a density of 5000 cells per well on 96-well Corning Spheroid Microplate (Corning, NY, USA) and incubated at 37 °C under 5% CO2 for 5 days before performing experiments. The

(12) TRAIL signaling promotes entosis in colorectal cancerJournal of Cell BiologyNovember 1, 2021Emir Bozkurt, Heiko Düssmann, Manuela Salvucci, Brenton L. Cavanagh, Sandra Van Schaeybroeck, Daniel B. Longley, Seamus J. Martin, Jochen H.M. PrehnHCT116 cells were seeded on 96-well spheroid plates (96-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate; Corning) at a density of 1,000 cells/well and grown in RPMI medium at 37°C with 5% CO2 for 48 h until

(13) Biological characteristics and metabolic profile of canine mesenchymal stem cells isolated from adipose tissue and umbilical cord matrixPLOS ONEMarch 4, 2021Romina Marcoccia, Salvatore Nesci, Barbara Merlo, Giulia Ballotta, Cristina Algieri, Alessandra Pagliarani, Eleonora IaconoP3 cells were cultured in multiwell Corning 96-well Black/ Clear Round Bottom Ultra- Low Attachment Spheroid Microplate (5000 cells/25 μL drop) for 24 h. This method provides information about the direct cell-cell adhesion

(14) A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroidsMolecular Systems BiologyDecember 26, 2020Vito RT Zanotelli, Matthias Leutenegger, Xiao‐Kang Lun, Fanny Georgi, Natalie de Souza, Bernd Bodenmillerusing a dilution series.Seed 100ul of cell suspension to each well of the 96‐well Ultra‐Low Attachment Spheroid Microplate (Corning).Spin plate 4 min at 250 g.Optional: image plate using an automated bright‐field microscope

(15) Three-dimensional spheroid culture of canine hepatocyte-like cells derived from bone marrow mesenchymal stem cellsRegenerative TherapyDecember 1, 2020Suguru Nitta, Masaharu Hisasue, Yu Horiguchi, Yoko Yamada, Kaoruko Kikuchi, Takeaki Kubo, Hirotaka Igarashi, Sakurako Neoblue dye, seeded at 3000 cells/well in a sterile 96-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate (Corning, New York, USA), and cultured at 37 °C under 5% CO2 for three days. During the culture, the

(16) Molecular subtype-specific responses of colon cancer cells to the SMAC mimetic BirinapantCell Death & DiseaseNovember 1, 2020Michael Fichtner et al.individual wells of a 96-Well spheroid plate (96-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate, Corning, UK) at a density of 1000 cells/well and grown in the medium as described earlier but supplemented

(17) Real-time sensing of MAPK signaling in medulloblastoma cells reveals cellular evasion mechanism counteracting dasatinib blockade of ERK activation during invasionNeoplasiaOctober 1, 2020Marc Thomas Schönholzer, Jessica Migliavacca, Elena Alvarez, Karthiga Santhana Kumar, Anuja Neve, Alexandre Gries, Min Ma, Michael A. Grotzer, Martin Baumgartnerof dasatinib (80 µM). i384 well plates 250 DAOY cells in 20 μl per well were seeded in a 384 well Corning spheroid microplate (Corning Incorporated, NY, USA). After 24–48 h, FDA-approved drugs were dispensed with a hp d300 digital

(18) Utility of Three-Dimensional Cultures of Primary Human Hepatocytes (Spheroids) as Pharmacokinetic ModelsBiomedicinesSeptember 23, 2020Kenta Mizoi, Hiroshi Arakawa, Kentaro Yano, Satoshi Koyama, Hajime Kojima, Takuo Ogiharaafter seeding [49] 8 Hepatotoxicity To evaluate the role of Kupffer cells in DILI using co-culture spheroids Spheroid microplate (CORNING) Culture for 15 days after seeding [50] 9 Hepatotoxicity Evaluation of two 3D spheroid models

(19) Cell shape, and not 2D migration, predicts extracellular matrix-driven 3D cell invasion in breast cancerAPL BioengineeringJune 1, 2020Janani P. Baskaran et al.assay Cells were seeded in low-attachment plates in media (Corning™ 96 Well Ultra-Low Attachment Treated Spheroid Microplate, 12–456-721), followed by centrifugation at 3000 rpm for 3 min to form spheroids. Spheroids were grown

(20) High-resolution tomographic analysis of in vitro 3D glioblastoma tumor model under long-term drug treatmentScience AdvancesMarch 6, 2020Mehmet S. Ozturk, Vivian K. Lee, Hongyan Zou, Roland H. Friedel, Xavier Intes, Guohao Daiuntil they are ready to be seeded. To create GBM spheroid, 1000 to 5000 GBM cells were plated into a Corning Spheroid Microplate and then cultured for 7 to 14 days until the spheroids reach the desired diameter (>400 μm). Collagen

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