SP6 RNA Polymerase from New England Biolabs

Product Specs
ItemSP6 RNA Polymerase
CompanyNew England Biolabs
Price $74.00 Inquire
Catalog NumberM0207S
Quantity2000 units
Concentration20u/µl
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to incorporate 1 nmole ATP into an acid-insoluble form in 1 hour at 40°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Evolution of neurohormone function revealed by actions of kisspeptin-type peptides in an echinodermBMC BiologyFebruary 18, 2026Tabinda Islam, Luis A. Yañez-Guerra, Dean C. Semmens, Riley T. Beskeen, Michaela Egertová, Maurice R. ElphickNo. M0251S) for the antisense probe or 5 U/μl of T7 RNA polymerase for the sense probe (NEB, Cat. No. M0207S). After subsequent ethanol precipitation and centrifugation, the final concentration of the probes was

(2) A Y-linked duplication of anti-Mullerian hormone is the sex determination gene in threespine sticklebackPLOS GeneticsNovember 4, 2025Matthew J. Treaster, Jenny McCann, Kyra S. Solovei, Ryan J. Palmieri, Michael A. Whiteusing T7 (New England Biolabs M0251S), T3 (New England Biolabs M0378S), or SP6 (New England Biolabs M0207S) RNA polymerase according to manufacturer’s instructions, substituting 0.2 mM of UTP for DIG-labeled

(3) Competing Programs Shape Cortical Sensorimotor-Association Axis DevelopmentbioRxivJune 27, 2025Jeremiah Tsyporin, Menglei Zhang, Cai Qi, Ashlea Segal, Thomas Finn, Hyojin Kim, Sang-Hun Choi, Xinyun Li, Sara Bandiera, Ivan Pavlovic, et al.The digoxigenin-labelled probes were synthesized from the linearized templates either by SP6 (NEB, M0207S), T7 (NEB, M0251S) and T3 (NEB, M0378S) RNA polymerases, respectively and RNA labelling mix (Roche,

(4) In mice, discrete odors can selectively promote the neurogenesis of sensory neuron subtypes that they stimulateElifeJune 18, 2025Kawsar Hossain, Madeline Smith, Karlin E Rufenacht, Rebecca O’Rourke, Stephen W Santoroprotein",T7 RNA Polymerase,NEB,Cat# M0251L, "Peptide, recombinant protein",Sp6 RNA Polymerase,NEB,Cat# M0207S, "Peptide, recombinant protein",RQ1 RNase-Free DNase,Promega,Cat# M6101, Sequence-based reagent,DIG-labeled

(5) A Y-linked duplication of anti-Mullerian hormone is the sex determination gene in threespine sticklebackbioRxivMay 25, 2025Matthew J. Treaster, Jenny McCann, Kyra S. Solovei, Ryan J. Palmieri, Michael A. Whiteusing T7 (New England Biolabs M0251S), T3 (New England Biolabs M0378S), or SP6 (New England Biolabs M0207S) RNA polymerase according to manufacturer’s instructions, substituting 0.2 mM of UTP for DIG-labeled

(6) Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafishThe EMBO JournalApril 25, 2025Yizhuang Zhang, Jiasheng Wang, Hailing Fang, Shuqi Hu, Boya Yang, Jiayi Zhou, Raphaëlle Grifone, Panfeng Li, Tong Lu, Zhengyang Wang, et al.analog (NEB, S1407S), 0.5 mM ATP, 0.5 mM CTP, 0.5 mM UTP, 0.1 mM GTP, 20 U SP6 RNA polymerase (NEB, M0207S), 20 U RNA inhibitor (Thermo Scientific, EO0381), and 1× RNA polymerase buffer. The mixture was incubated

(7) Dyrk1a is required for craniofacial development in Xenopus laevisDevelopmental BiologyJuly 1, 2024H. Katherine Johnson, Stacey E. Wahl, Fatmata Sesay, Larisa Litovchick, Amanda JG. Dickinsonlabeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB, M0207S; sense probe). Embryos used for in situ hybridization were fixed in 4% PFA, dehydrated in a methanol

(8) Evolutionarily conserved Wnt/Sp5 signaling is critical for anterior-posterior axis patterning in sea urchin embryosiScienceJanuary 19, 2024Sujan Gautam, Jennifer L. Fenner, Boyuan Wang, Ryan C. RangeSuperScript IV First-Strand Synthesis System,Invitrogen,11483188001 pGEMT®-easy,Promega,A137A SYBER green qPCR mix,BioRad,1725121 Sp6 and T7 RNA Polymerase,New England Biolabs,M0207S and M0251S Tyramide Signaling Amp Kit,Perkin Elmer,Discontinued product DIG RNA Labeling Mix,Roche,11277073910 Fluorescein RNA Labeling Mix

(9) A histological protocol for quantifying the birthrates of specific subtypes of olfactory sensory neurons in miceSTAR ProtocolsSeptember 1, 2023Kawsar Hossain, Madeline Smith, Stephen W. SantoroR0104S BamHI,NEB,Cat# R0136S NotI,NEB,Cat# R0189S T7 RNA polymerase,NEB,Cat# M0251L Sp6 RNA polymerase,NEB,Cat# M0207S RQ1 RNase-free DNase,Promega,Cat# M6101 ,, Critical commercial assays,Critical commercial assays,Critical

(10) Evolution of naturally arising SARS-CoV-2 defective interfering particlesCommunications biologyOctober 27, 2022Girgis S, Xu Z, Oikonomopoulos S, Fedorova AD, Tchesnokov EP, Gordon CJ, Schmeing TM, Götte M, Sonenberg N, Baranov PV, et al.translations, a FF/EMCV/Ren bicistronic reporter mRNA was transcribed in vitro using SP6 RNA Polymerase (NEB, #M0207S) and co-transcriptionally capped with m7GpppG RNA Cap Analog (NEB, #S1404S). Nuclease-treated rabbit

(11) Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular modelCell reportsMay 31, 2022Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X, et al.RRID:AB_2336382 Immobilon Western chemiluminescent HRP substrate,Millipore,Cat#WBKLS0500 Sp6 RNA polymerase,NEB,Cat#M0207S SacI-HF,NEB,Cat#R3156S ClaI,NEB,Cat#R0197S AatII,NEB,Cat#R0117S BsHII,NEB,Cat#R0199S Human brain microvascular endothelial cell lysate

(12) Protein Expression of the Microglial Marker Tmem119 Decreases in Association With Morphological Changes and Location in a Mouse Model of Traumatic Brain InjuryFrontiers in Cellular NeuroscienceFebruary 10, 2022Domenico Mercurio, Stefano Fumagalli, Martin K-H Schafer, Jordi Pedragosa, Lionel Dejeumen Claude Ngassam, Verena Wilhelmi, Sarah Winterberg, Anna M. Planas, Eberhard Weihe, Maria-Grazia De Simoniwere synthesized in sense and anti-sense orientation by IVT with T7 and SP6 RNA polymerases (M0251S, M0207S, New England Biolabs ®, Frankfurt, Germany), respectively, using UTPαS[35S] (NEG039C001MC, PerkinElmer,

(13) Somite morphogenesis is required for axial blood vessel formation during zebrafish embryogenesiseLifeFebruary 9, 2022Paulissen E, Palmisano NJ, Waxman JS, Martin BL.,DIG RNA Labeling Mix,Roche,Cat# 11277073910, "Peptide, recombinant protein",SP6 RNA polymerase,NEB,Cat# M0207S, "Peptide, recombinant protein",T7 RNA polymerase,NEB,Cat# M0251S, "Chemical compound, drug",Tricaine-S

(14) Using an aquatic model, Xenopus laevis, to uncover the role of chromodomain 1 in craniofacial disordersgenesisFebruary 1, 2021Brent H. Wyatt, Thomas O. Raymond, Lisa A. Lansdon, Benjamin W. Darbro, Jeffrey C. Murray, John Robert Manak, Amanda J. G. Dickinsonwith either T7 RNA polymerase (NEB, cat # M0251S; antisense probe), or SP6 RNA polymerase (NEB, cat # M0207S; sense probe). The probe was then hydrolyzed by combining it (1 μg) with 40 mM sodium bicarbonate and

(15) Olfactory Stimulation Regulates the Birth of Neurons That Express Specific Odorant ReceptorsCell ReportsOctober 6, 2020Carl J. van der Linden, Pooja Gupta, Ashraful Islam Bhuiya, Kelci R. Riddick, Kawsar Hossain, Stephen W. Santoro88803 Tamoxifen,Alfa Aesar,Cat# J63509 T7 RNA Polymerase,NEB,Cat# M0251L Sp6 RNA Polymerase,NEB,Cat# M0207S RQ1 RNase-Free DNase,Promega,Cat# M6101 Vectashield,Vector Laboratories,Cat# H1000 Hibernate AB Complete

(16) Molecular barcoding of native RNAs using nanopore sequencing and deep learningGenome ResearchSeptember 1, 2020Martin A. Smith, Tansel Ersavas, James M. Ferguson, Huanle Liu, Morghan C. Lucas, Oguzhan Begik, Lilly Bojarski, Kirston Barton, Eva Maria NovoaT7-Flash Transcription kit (Lucigen ASF3507). Sequin IVT sequences were produced using SP6 Polymerase (NEB M0207S), following the manufacturer's recommendations. Each IVT reaction was incubated for 4 h at 42°C for

(17) Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostomeScientific ReportsDecember 1, 2018Luis Alfonso Yañez-Guerra, Jérôme Delroisse, Antón Barreiro-Iglesias, Susan E. Slade, James H. Scrivens, Maurice R. Elphickthe antisense probe or 5 U/μl of SP6 RNA polymerase for the sense probe (NEB, Ipswich, USA, Cat. No. M0207S). The mixture was incubated for 2 hours at 37 °C to allow the in vitro transcription. The synthesised

(18) Manduca Contactin Regulates Amyloid Precursor Protein-Dependent Neuronal MigrationThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 17, 2016Ramaker JM, Swanson TL, Copenhaver PF.extraction, and antisense probes were generated using T7 or SP6 RNA polymerase (New England Biolabs, #M0207S and #M02515), plus dithiothreitol, digoxigenin-11-UTP (DIG) labeling mix (Roche, #11277073910), transcription

(19) Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovariesNature ProtocolsNovember 1, 2013Sandra G Zimmerman, Nathaniel C Peters, Ariel E Altaras, Celeste A Bergcat. no. 11031163001), T7 (New England Biolabs, cat. no. M0251S) or SP6 (New England Biolabs, cat. no. M0207S) Primers for amplifying genomic DNA (many suppliers, we use Operon) TOPO TA dual promoter kit (Invitrogen,

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