Agilent BioTek Gen5 Software for Detection from Agilent Technologies

Product Specs
ItemAgilent BioTek Gen5 Software for Detection
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberGen5 Software
QuantityEA
ApplicationsMicroplate Reading

Specifications/Features

All-in-one instrument control and data analysis software, enables US FDA 21 CFR Part 11 compliance

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Multi-omics dissection of metabolic hijacking: Infectious bronchitis virus orchestrates lipid-centric replication through PPAR-TGF-β crosstalkVirulenceDecember 31, 2026Kun Yan, Xiuling Wang, Zongyi Bo, Chengcheng Zhang, Mengjiao Guo, Xiaorong Zhang, Yantao Wuusing an ELx800 microplate reader (BioTek, USA), and the parameters were set using the accompanying Gen5 software. The PGE2 concentrations in the CEK culture supernatants were calculated based on the generated standard

(2) Identification and characterization of five anti-mitotic sesquiterpene lactones from Arnica cordifoliaPharmaceutical BiologyDecember 31, 2026Tanner C. Lockwood, David E. Williams, Layla Molina, Raymond J. Andersen, Roy M. Golsteyneach sample was measured from 300 to 700 nm using a Biotek Epoch microplate spectrophotometer using Gen5 software (Agilent Technologies), with a step of 2 nm between reads. Absorbance data were blanked to the MeOH

(3) High-throughput analyses of Phocaeicola vulgatus reveal fitness determinants for gut colonization and during colitisGut MicrobesDecember 31, 2026Nazik M. Elmekki, Michael J. Coyne, Katia Flores, Leonor García-Bayona, Laurie E. Comstockmeasured every 30 min (shaking for 10 s prior to reading) using a Biotek Epoch 2 plate reader with Gen5 software (Agilent, Santa Clara, CA). P. vulgatus CL10T00C06 RB-TnSeq library construction E. coli AMD776 was

(4) Habitual coffee intake shapes the gut microbiome and modifies host physiology and cognitionNat CommunApril 21, 2026Serena Boscaini, Thomaz F. S. Bastiaanssen, Gerard M. Moloney, Federica Bergamo, Laila Zeraik, Caroline O'Leary, Aimone Ferri, Maha Irfan, Maaike van der Rhee, Thaïs I. F. Lindemann, et al.Buffer + 100 μL of saliva sample). The absorbance was measured with Synergy (BioTek) plate reader and Gen5 software, with a lower detection limit of 57 pg/mL. Each saliva sample was tested in duplicate, and the average

(5) Nanostructured Lipid Carriers Enhance Ciprofloxacin Antibacterial Activity Through Diffusion-Controlled Release and Modulation of Bacterial Growth KineticsPharmaceuticsApril 17, 2026Javiera Carrasco-Rojas, Felipe I. Sandoval, Javiera Solas-Soto, Christina M. A. P. Schuh, Lorena Rubio-Quiroz, Carlos F. Lagos, Francisco Arriagada, Andrea C. Ortizwas recorded at 570 nm using a SYNERGY H1 microplate reader (BioTek, Winooski, VT, USA) operated with Gen5 software. 2.12. Statistical Analysis All experiments were performed with at least three independent replicates

(6) Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cellsiScienceApril 17, 2026Laura Unmuth, Britta Lipinski, Alicia Hoerr, Julia Harwardt, Enrico Guarnera, Michal Szczepek, Stefan Becker, Andreas Menrad, Patrick Scheerer, Andreas Evers, et al.Data Analysis Software 8.0,ForteBio,RRID:SCR_023267 IntelliCyt ForeCyt Software 10.1,Sartorius,N/A Gen5 Software 3.15.15,BioTek,RRID:SCR_017317 Incucyte Software v2025A,Sartorius,RRID:SCR_026298 molecular modeling

(7) Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA®) platform elicits enhanced humoral and cellular immune responsesFront ImmunolApril 15, 2026Daria L. Ivanova, Matthew S. Lewis, Annie B. Caplan, Isabella R. Walters, Emma M. Snyder, Keith A. Strand, Lorena R. Antunez, Sineenart Sengyee, Sarah B. Weiby, Federico Urbano-Munoz, et al.nm. The standard curves and interpolated data for each plate were independently generated using the Gen5 software (Agilent, Santa Clara, CA, USA). 2.3.2 Anti-CPS and anti-Hcp1 Serum from terminally bled mice was

(8) Klebsiella pneumoniae factors enhancing bacteremia have distinct contributions to macrophage-mediated, oxidative, and nitrosative stress resistanceInfection and ImmunityApril 15, 2026Alexis E. Wilcox, Catherine J. Andres, Michael A. Bachman, Caitlyn L. Holmes107 CFU/mL in LB broth or M9 salts (Gibco) containing 0.4% mannitol. An Eon microplate reader with Gen5 software (Version 2.0, BioTek, Winooski, VT, USA) measured OD600 every 15 min. Plates were incubated at 37°C

(9) Abnormal intestinal microbial colonization in prenatally stressed offspring is related to lung and intestinal cytokine expressionFront MicrobiolApril 9, 2026Audrey F. Duff, Michael T. Baileyquality (A260/A280) were verified using the Take3 application “Nucleic Acid Quantification” within the Gen5 software on the Synergy HTX Multi-mode Microplate Reader (Agilent BioTek, Santa Clara, CA), and cDNA was synthesized

(10) Aortic carboxypeptidase-like protein potentiates β1 integrin signaling in mesenchymal progenitorsLife Sci AllianceApril 8, 2026Cheyanne L Frosti, Diana Yeritsyan, Matthew D Laynefrom assembling collagen fibrils. Measurements were taken at 410 nm every 30 s for 45 min using BioTek Gen5 software (v3.11). Adhesion assays A 96-well plate (12565501; Fisherbrand) was coated with 0.05 mg/ml type I

(11) Proviral NUP153 binding to viral proteins and RNA regulates structural-nonstructural protein ratios in orthoflavivirus infectionNat CommunApril 8, 2026Marie B. A. Peters, Richard Lindqvist, Eszter Kassa, Wai-Lok Yau, Pallabi Sengupta, Isabell Niedermoser, Gisa Gerold, Nasim Sabouri, Ylva Ivarsson, Richard Lundmark, et al.dilution buffer. Cells were washed three times with PBS and imaged and analyzed with Cytation 5 and BioTek Gen5 software (Agilent). Affinity purification-mass spectrometry HEK293T cells were seeded in T-175 flasks and transfected

(12) Novel Palmitoyl Pentapeptides: Self-Assembly, Collagen Stimulation, Antioxidant Activity, and Influence of Skin Microbiota HealthACS OmegaApril 7, 2026Juliane N. B. D. Pelin, Lucas R. de de Mello, Raquel Allen Garcia Barbeto Siqueira, Matheus de de Souza Alves, Valeria Castelletto, João Francisco Almeida, Jani Seitsonen, Patricia Santos Lopes, Vânia Leite-Silva, Newton Andreo-Filho, et al.ensure homogenization. Fluorescence was measured using a microplate reader (BioTek Synergy HT Multimode, Gen5 software) with an excitation at 485 nm and emission at 538 nm. The fluorescence intensity of DCF was used as

(13) High stability of the genome of Akkermansia muciniphila Muc T under long-term culturing conditionsMicrobiology SpectrumApril 7, 2026Kate Ligthart, Janneke Elzinga, Anneleen Segers, Hauke Smidt, Willem M. de Vosstopped with an equal volume of 0.5 M H2SO4. The absorbance at 450 nm was measured (Agilent BioTek, Gen5 Software). Dunnett’s multiple comparisons tests were used to determine statistical significance. To further investigate

(14) Mitochondrial presequences harbor variable strengths to maintain organellar functionJournal of Cell BiologyApril 6, 2026Youmian Yan, Baigalmaa Erdenepurev, Thiago N. Menezes, Ian Collinson, Natalie M. NiemiBioTek Synergy LX Multimode Reader (Agilent Technologies) using the luminescence filter and BioTek Gen5 software (Agilent Technologies, version 3.11). Measurements were taken every 8 seconds, and the plate was shaken

(15) Yeast Phenomic Analysis Reveals DNA Repair, pH Homeostasis, and Ribosomal Biogenesis as Modulators of Anticancer Ruthenium Complex KP1019Int J Mol SciApril 4, 2026Amanda F. Bible, Jackson S. Blackman, John W. Rodgers, Samuel R. Gary, Megan Rainey, Mary E. Miller, Alexander Stepanov, John L. Hartman 4th, Laura K. Stultz, Pamela K. Hansonpre-treatment of microtiter plate lids [121]. A BioTek Synergy LX plate reader controlled by Biotek Gen5 software version 3.10.06 (Agilent Technologies, Santa Clara, CA, USA) held the plates at room temperature and

(16) The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37Cell ReportsApril 1, 2026Joana R. Loureiro, Ana F. Castro, Ana S. Figueiredo, Ana Eufrásio, Ashutosh Dhingra, Mafalda Galhardo, Hugo Marcelino, Catarina C. Rodrigues, Paula Sampaio, Maria Azevedo, et al.activity measurement. Luminescence produced was measured in the Synergy 2 plate reader (BioTek), using the Gen5 software. In vitro silencer assays The thymidine kinase (TK) promoter was cloned in pGL4.23 GW[luc2/minP] (#60323;

(17) UVA Irradiation Promotes ROS-Mediated Formation of the Common Deletion in Mitochondrial DNALife (Basel)April 1, 2026Gabriele A. Fontana, Navnit K. Singh, Nadezhda Rotankova, Antonia Eichelberg, Michela Di Filippo, Michael R. MacArthur, Susanne Heldmaier, Franziska Wandrey, Hans-Dietmar Beer, Shana J. Sturla, et al.automated cell counting using a Cytation 5 Cell Imaging Reader (BioTek, Agilent Technologies, Winooski, VT, USA) and Gen5 software (BioTek). 2.13. Dot Blot Dot blots were performed on mtDNA extracted from UV-irradiated and unexposed

(18) From grass to legume: employing Poa pratensis-associated bacteria to improve soybean performance under salinity stressFront Plant SciApril 1, 2026Nadia Monjezi, Hamid Reza Eisvand, Sima Abdoli, Donald L. Smithtolerance of the bacterial isolates was assessed using a microplate reader (Cytation 5, BioTek, USA) with Gen5 software. A 96-well microplate was prepared, with each well containing 200 µL of culture composed of 20 µL bacterial

(19) Ultracentrifugation and Ultrafiltration Differentially Alter the Composition and Functionality of the Biomolecular Corona of Extracellular VesiclesJ Extracell BiolApril 1, 2026Frederic St-Denis-Bissonnette, Hala Halabi, Marybeth Creskey, Melanie Kirkby, Xu Zhang, Dylan Burger, Lisheng Wang, Jessie R. Lavoiewas measured after 90 min using a plate reader (BioTek Synergy H1 Multimode Reader; cat#SH1M2G‐SN; Gen5 software V3.14). Results were averaged and corrected for background. 2.12 Data Availability (EV‐TRACK) All

(20) Protocol for automated quantification of neuronal cells using the Agilent BioTek Cytation system and Gen5 neurite outgrowth moduleSTAR ProtocApril 1, 2026Aline Broeglin, Olivier Buetzberger, Rebecca Mongeon, Aurélien Riou, Anne Eckert, Amandine Grimmtreatments and cell models were selected to demonstrate the sensitivity and accuracy of the Agilent BioTek Gen5 software Neurite Outgrowth module in detecting subtle alterations in neurite outgrowth parameters induced by

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