Forecyt® software from Sartorius

Product Specs
ItemForecyt® software
CompanySartorius
Price Get Pricing Get Quote for this Product
Catalog NumberiQue Forecyt® Software
ApplicationsHigh-Throughput Screening (HTS) by Cytometry
Quantity1 pack

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Local Office

Sartorius

5 Orville Drive

Bohemia, NY 11716

United States

Company Profile

International Office

Sartorius

Otto-Brenner-Straße 20

37079 Göttingen

Germany

Phone: +49 551 308 0

Company Profile

Website: www.sartorius.com

(1) Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullinationCell Reports MethodsApril 20, 2026Sophia Laposchan, Erik Riedel, Andrew Flatley, Selina Pasquero, Regina Feederle, Chien-Yun Leearginine instead of citrulline was used for negative screening. Antibody binding was analyzed using ForeCyt software (Sartorius). Hybridoma cells of clones CIT 12A2 (rat IgG2c) and CIT 21F10 (rat IgG2b) were subcloned

(2) Breast milk antibody Fc signatures track with HIV transmission during breastfeeding in ART-naïve mothersiScienceApril 17, 2026Nadege Nziza, Wonyeong Jung, Yunjoo Lee, Jeff C. Hsiao, Tina Chen, Fan Li, Caroline Atyeo, Douglas A. Lauffenburger, Louise Kuhn, Nicole H. Tobin, et al.cells,, ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Intellicyt ForeCyt Software,Sartorius,https://intellicyt.com/products/software/ Python programming language,Version 3.13.1,https://www.python.org/ R

(3) Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cellsiScienceApril 17, 2026Laura Unmuth, Britta Lipinski, Alicia Hoerr, Julia Harwardt, Enrico Guarnera, Michal Szczepek, Stefan Becker, Andreas Menrad, Patrick Scheerer, Andreas Evers, et al.v10.10,Tree Star,RRID:SCR_008520 ForteBio Data Analysis Software 8.0,ForteBio,RRID:SCR_023267 IntelliCyt ForeCyt Software 10.1,Sartorius,N/A Gen5 Software 3.15.15,BioTek,RRID:SCR_017317 Incucyte Software v2025A,Sartorius,RRID:SCR_026298 molecular

(4) Epitope-focused discovery of SARS-CoV-2 antibodies that potently neutralize Omicron variantsNature MicrobiologyMarch 12, 2026Seth J. Zost, Naveenchandra Suryadevara, Lauren E. Williamson, Suzanne M. Scheaffer, Elad Binshtein, Cameron D. Buchman, Nicole V. Johnson, Nicholas J. Catanzaro, Silvia Ravera, Nathaniel S. Chapman, et al.competition signal relative to a standard curve of a control human IgG. Data were analysed using the ForeCyt software v.6.2 (Sartorius). ELISA dose–response binding assays Microtitre plates (384-well) were coated with

(5) Liver metastases dampen IL18-driven γδ T cell activity and immunotherapy responsiveness in colorectal cancerCell Reports MedicineFebruary 17, 2026Allard W.J. van Renterghem, Miguel Parra-Martinez, Maartje Witsen, Karlijn Verkerk, Marit Steur, Laurien J. Zeverijn, Krijn K. Dijkstra, Leila Akkari, Emile E. Voestfrom whole blood samples (Streck). Readouts were perfomred using a iQue 3 (Sartorius) with standard Forecyt software. Analyses of flow cytometry data was performed in the provided LEGENDplex Data Analysis Software Suite. For

(6) Epstein-Barr-virus-specific functional antibody signatures in the context of nasopharyngeal carcinoma developmentMedJanuary 9, 2026Vicky Roy, Benjamin P. Kellman, Wan-Lun Hsu, Nadege Nziza, Lily Parker, Daritza Germosen, Riley Bonifer, Ruth M. Pfeiffer, Kelly J. Yu, Birgitta Michels, et al.RRID: CVCL_0006 Software and algorithms,Software and algorithms,Software and algorithms Intellicyt ForeCyt Software,Sartorius,https://intellicyt.com/products/software/ Python programming language,Version 3.13.1,https://www.python.org/ R

(7) Fine-tuning affinity and spacer design enhances T cell potency in DLL3 and BCMA CAR T cellsMAbsDecember 12, 2025Nicholas Mazzanti, Ninkka Tamot, Andrea Francese, Jinquan Luo, M. Jack Borrok, Julie Rossillo, Joseph Plummer, Gauri Anand Patwardhan, Chi Shing Sum, Michael Ports, et al.binding to live tumor cells was measured and reported as the mean fluorescent intensity (MFI) using Forecyt software. CAR design Affinity variant scFv constructs were paired with one of three spacer domains for CAR

(8) The CHCHD2-CHCHD10 protein complex is modulated by mitochondrial dysfunction and alters lipid homeostasis in the mouse brainCell Death DisOctober 6, 2025Jule Gerlach, Paola Pireddu, Xiaoqun Zhang, Simon Wetzel, Mara Mennuni, Dusanka Milenkovic, Hendrik Nolte, Fernanda da Silva Rodrigues, Niclas Branzell, Ibrahim Kaya, et al.CHCHD2 protein captured on beads (3D-Carboxy, PolyAn, Berlin). Antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were further validated by Western blot on mouse wildtype and Chchd2

(9) Design, Synthesis and Preclinical Evaluation of a Brain-Permeable PET Tracer for P2Y12 Receptor Imaging in the BrainJournal of Medicinal ChemistryAugust 14, 2025Emanuel Joseph, Lea H. Kunze, Rebecca Schaefer, Giovanna Palumbo, Benjamin Kugelmann, Stephan Wagner, Sven Lammich, Regina Feederle, Michael Willem, Rudolf A. Werner, et al.isotype-specific monoclonal mouse-antirat IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were further validated in capture ELISA, Western blot and immunohistochemistry.

(10) A niche driven mechanism determines response and a mutation-independent therapeutic approach for myeloid malignanciesCancer cellJune 9, 2025Mosialou I, Ali AM, Labella R, Bisikirska B, Cuesta-Dominguez A, Vgenopoulou P, Reyes I, Rao SM, Wang A, Luo N, et al.to detect bound D1. Bound APC fluorescence was analyzed using an IntelliCyt® iQue Screener PLUS and ForeCyt Software (Sartorius) and curve fitting and apparent KD values determined using GraphPad software. The level of

(11) The VLDLR entry receptor is required for the pathogenesis of multiple encephalitic alphavirusesCell ReportsOctober 22, 2024Sathvik Palakurty, Saravanan Raju, Alan Sariol, Zhenlu Chong, Ngan Wagoner, Hongming Ma, Ofer Zimmerman, Lucas J. Adams, Camille Carmona, Zhuoming Liu, et al.min at 4°C. Following washing steps, cells were analyzed on an iQue3 flow cytometer (Sartorius) and Forecyt software. For some experiments, cells stained with anti-Flag-A647 were sorted equivalent expression on a MACSQuant Tyto

(12) ISB 2001 trispecific T cell engager shows strong tumor cytotoxicity and overcomes immune escape mechanisms of multiple myeloma cellsNature CancerSeptember 11, 2024Laura Carretero-Iglesia, Olivia J. Hall, Jérémy Berret, Daniela Pais, Carole Estoppey, Myriam Chimen, Thierry Monney, Jeremy Loyau, Cyrille Dreyfus, Julie Macoin, et al.performed by flow cytometry (IntelliCyt iQueScreenerPlus, Sartorius) and analysis was performed using ForeCyt Software (Sartorius). T cell activation was measured as the percentage of live CD4+ or CD8+ T cells expressing

(13) Designing patient-oriented combination therapies for acute myeloid leukemia based on efficacy/toxicity integration and bipartite network modelingOncogenesisMarch 1, 2024Mehdi Mirzaie, Elham Gholizadeh, Juho J. Miettinen, Filipp Ianevski, Tanja Ruokoranta, Jani Saarela, Mikko Manninen, Susanna Miettinen, Caroline A. Heckman, Mohieddin Jafarimin at room temperature (Table S3). Cells were analyzed with the iQue3 screener (Sartorius, Germany). ForeCyt software (Sartorius) was used to analyze the remaining viable cells and data normalized to the number of viable

(14) Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritisiScienceFebruary 16, 2024Bowman KA, Wiggins CD, DeRiso E, Paul S, Strle K, Branda JA, Steere AC, Lauffenburger DA, Alter G.algorithms,Software and algorithms ,, R programming language,Version 4.0.3,https://www.r-project.org/ ForeCyt software,Sartorius,https://www.sartorius.com/en/products/flow-cytometry/flow-cytometry-software ,, Other,Other,Other ,, "Fluospheres™ NeutrAvidin™-Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids

(15) Interactions of the anti-FcRn monoclonal antibody, rozanolixizumab, with Fcγ receptors and functional impact on immune cells in vitromAbsJanuary 1, 2024Qureshi OS, Sutton EJ, Bithell RF, West SM, Cutler RM, McCluskey G, Craggs G, Maroof A, Barnes NM, Humphreys DP, et al.antibodies and Fcs was quantified using an iQUE3 (Sartorius) flow cytometer. Data were analyzed using Forecyt software (Sartorius) and mean fluorescence intensity (MFI) was used to represent binding of the test antibodies

(16) Refinement of an ovine-based immunoglobulin therapy against SARS-CoV-2, with comparison of whole IgG versus F(ab')2 fragmentsSci RepAugust 25, 2023Stephen Findlay-Wilson, Linda Easterbrook, Sandra Smith, Neville Pope, Matthew Aldridge, Gareth Humphries, Holger Schuhmann, Didier Ngabo, Emma Rayner, Ashley Otter, et al.buffer, the samples were resuspended in 40 µl HBSS and analysed using an iQue Screener Plus® with iQue Forecyt® software (Sartorius, Germany). For each sample, a minimum of 100 beads were collected. Conjugated beads were

(17) Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivoActa neuropathologica communicationsJuly 11, 2023Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, et al.isotype-specific monoclonal rat anti-mouse IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius, Germany). Clones were further validated and selected for up-scaling by sandwich ELISA. Briefly,

(18) Ex vivo venetoclax sensitivity testing predicts treatment response in acute myeloid leukemiaHaematologicaJuly 1, 2023Heikki Kuusanmäki, Sari Kytölä, Ida Vänttinen, Tanja Ruokoranta, Amanda Ranta, Jani Huuhtanen, Minna Suvela, Alun Parsons, Annasofia Holopainen, Anu Partanen, et al.Flow cytometry data were acquired using an iQue Screener PLUS instrument (Sartorius, Germany), and ForeCyt software (Sartorius) was used to analyze the cells. The gating strategy is presented in Online Supplementary

(19) Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing NeuritinAntibodies (Basel)April 7, 2023Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Andrew Flatley, Regina Feederle, Günter Bernhardt, Hristo Georgievantibodies (anti-IgG3 (clone 2E.6, HB-128, ATCC), all other in house), antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were further analyzed by flow cytometry analysis and selected hybridoma

(20) Syrian hamster convalescence from prototype SARS-CoV-2 confers measurable protection against the attenuated disease caused by the Omicron variantPLoS pathogensApril 1, 2023Ryan KA, Bewley KR, Watson RJ, Burton C, Carnell O, Cavell BE, Challis A, Coombes NS, Davies ER, Edun-Huges J, et al.buffer, the samples were resuspended in 40 μL HBSS and analysed using an iQue Screener Plus with iQue Forecyt software (Sartorius, Germany). For each sample, a minimum of 100 beads were collected. Conjugated beads were

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Forecyt® software from Sartorius

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Forecyt® software from Sartorius

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