Fig 1: DNA damage and repair after exposure to X-ray. FaDu derivatives. ((a) control, (b) shAPE1 + shPRMT5, (c) shAPE1, and (d) shPRMT5) were irradiated at 4.5 Gy X-ray and incubated for the indicated time. The extents of DSBs were then analyzed with a comet assay in neutral pH conditions, and their tail moment values were calculated using CometAssay software (R&D system/Trevigen). (e–p) Fluorescent microscope images of the comet assay samples. (e–g) FaDu wild-type, (h–j) shAPE1 + shPRMT5, (k–m) shAPE1, (o,p) shPRMT5. (e,h,k,n) no X-ray, (f,i,l,o) 30 min after 4.5 Gy X-ray exposure, and (g,j,m,p) 24 h after 4.5 Gy X-ray.