CFX Maestro™ Software from Bio-Rad

Product Specs
ItemCFX Maestro™ Software
CompanyBio-Rad
Price $1,289.00Supplier Page View Company Product Page
Catalog Number12004110

Specifications/Features

Operating system: Minimum: Windows 7, Mac OS X El Capitan
Recommended: Windows 10, Mac OS Sierra, Minimum: Windows 7
Recommended: Windows 10

Hard drive space: Minimum: 10 GB
Recommended: 20 GB

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Contrasting functions of CD73 and adenosine in CD8+ T-cell exhaustion during antitumor immunityOncoimmunologyDecember 31, 2026Juan Saavedra-Almarza, Solange Gouët, Felipe Malgue, Catalina Bascuñan, Andrés Hernández-Oliveras, Joaquín De la Rosa, Javiera Reyes-Alvarez, Camilo Villaman, José Corrales-Bermúdez, Justine Castañeda, et al.shown in Table 2. The reaction was performed using a Bio-Rad CFX Connect thermal cycler and Bio-Rad CFX Maestro software. Relative expression was calculated using the Pfaffl method. Adoptive transfer experiments To purify

(2) PARP7 inhibition and a STING agonist potentiate radiation-induced immunogenicity in glioblastomaOncoimmunologyDecember 31, 2026Luca Lippert, Ava Kleinwächter, Anna Ohradanova-Repic, Filip Horvat, Lisa-Marie Appel, Lena Walch, Niccolò Bragato, Jakob Wimmer, Hanna Wopfner, Anna Röhrer, et al.cDNA was diluted 1:5 in H2O. qPCR was performed on a Bio-Rad CFX Opus 96 cycler operated by Bio-Rad CFX Maestro software (Version 2.3) using iQTM Multiplex Powermix (Bio-Rad). IFNB1, CXCL10, PD-L1 (CD274), IL-6 (IL6), TNFα

(3) A naturalized gut microbiome interacts with dietary fibers to protect against colonic inflammationGut MicrobesDecember 31, 2026Signe Birkeland, Ingerid Rohde Mæhlum, Marte Senneset, Ingrid Wik Taxerås, Lars Snipen, Henriette Markov Arnesen, Preben Boysen, Harald Carlsenplate variability. Quantification cycles (Cq) were determined with CFX Maestro™ Software (Bio-Rad, #12004110). Primer sequences and annealing temperatures are listed in Supplementary Table S4. Relative gene expression analysis

(4) Comparison of pathogen and resistance profiles in bloodstream infections using conventional methods and multiplex RT-PCRMolecular Biology ReportsDecember 1, 2026Derya Çakır Erdoğan, Serpil Semiha Çuğlan, Gülşah Ece Özmerdiven, Faruk Çelik, Arzu İrvemtarget DNA regions using the Bio-Speedy® Sepsis RT-qPCR MX-30 S Panel, and results were analyzed with CFX Maestro software (Bio-Rad/Bioeksen, Türkiye). The PCR result was obtained 2–4 h after the positive blood culture signal. The

(5) Adenine base editor for knockout of proteins: a practical guide from design to analysis with updated MultiEditRbatchMolecular Therapy Nucleic AcidsJune 16, 2026Ella J. Eaton, Bryce J. Wick, Jeremy S. Chacón, Amy J. Wang, Mitchell Kluesner, Jackson T. Barnes, Bibekananda Kar, Minjing Wang, Matthew J. Johnson, Joseph G. Skeate, et al.(Bio-Techne). TaqMan gene expression assays were performed on a Bio-Rad CFX96 instrument and analyzed with CFX Maestro software v.5.3.022.1030, using probes listed in Table S5. Graphs were generated using GraphPad Prism (BD) v.10.4.1.

(6) Screening for novel chemical scaffolds targeting PCNA identifies the Hsp90alpha inhibitor SNX-2112Current Research in Structural BiologyJune 1, 2026Jennifer Jossart, Ning Ma, Pouya Haratipour, Caroline M. Li, Christopher Jang, Long Gu, Terrence E. O'Brien, Nagarajan Vaidehi, Linda H. Malkas, Robert J. Hickey, et al.each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound

(7) Characterization of Tiroler Bergkäse PDO cheese: A multimethodological approachFood Chemistry: Molecular SciencesJune 1, 2026Hannah Innerbichler, Alexander Trockenbacher, Alexander Höller, Sabine Scholl-Bürgi, Lorenzo Del Vecchio, Martina Cirlini, Jürgen König, Katrin BachqPCR assays were run on a CFX96 C1000 Touch Thermal Cycler (Bio Rad, Hercules, USA) and analyzed by CFX Maestro Software (Version 2.3–2021, Bio Rad, Hercules, USA). The primer pairs used in this study were purchased from

(8) Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathwayNeural Regeneration ResearchMay 1, 2026Yiming Huo, Bing Xiao, Haojie Yu, Yang Xu, Jiachen Zheng, Chao Huang, Ling Wang, Haiyan Lin, Jiajun Xu, Pengfei Yang, et al.instructions. U6 served as the reference gene. The cycle threshold (CT value) was obtained using Bio-Rad CFX Maestro software (version 4.0.2325.0418, Bio-Rad, Hercules, CA, USA). The 2–ΔΔCT method was used to calculate and analyze

(9) Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal LungCurr ProtocApril 30, 2026Evelyn Tran, Laurence St. Pierre, Diego Velarde, Bianca Dal Bó, Juan Ramon Alvarez, Kweku Ofosu-Asante, Nazarius S. Lamango, Yong Huang, Crystal N. Marconett, Zea Borok, et al.be found in Table 4. 13Run the qPCR samples on a Bio‐Rad CFX96 Real‐Time PCR thermocycler with CFX Maestro Software under the conditions tabulated in Table 5, based on the suggested cycling protocol from the manufacturer.PCR

(10) Purification and Transcriptomic Characterization of Hypertrophied Hepatic Stellate Cells From CDAHFD Mouse LiverFASEB JApril 30, 2026Marion Heckmann, Nour-El-Houda Djerir, Keola Greliche, Pierre-Henri Commere, Julien Fernandes, Guillaume Sarrabayrouse, Bernard Hainque, Pascal Bigey, Virginie Escriou, Céline Hoffmann95°C for 15 s and hybridization and elongation at 60°C for 1 min. Results were analyzed using Bio‐Rad CFX Maestro software. The stability of housekeeping genes was assessed on samples of sorted hypHSCs and qHSCs, using the

(11) Quantitative proteomic analysis of longissimus dorsi muscle in Chinese and Western pigs using DIA mass spectrometry with PRM validationPLoS OneApril 30, 2026Hanyu Cao, Xiaojin Li, Fei Xie, Changsheng Jiang, Mengmeng Jin, Ahmed H. Ghonaim, Man Ren, Qianqian Hu, Shenghe Lidemonstrated a linear dynamic range with R² > 0.99.Cq values were automatically determined by the Bio-Rad CFX Maestro™ software using baseline and threshold settings set to default. Technical replicates exhibiting a Cq standard

(12) Comprehensive genome characterization and expression analysis of the DUF4228 gene family in Sorghum (Sorghum bicolor L.) under salt stress conditionsPeerJApril 27, 2026Esra Alther, Awadalla Abdalla Abdelmula, Hind Abdelmonim Elsanosi, Salma Mostafa, Guisheng Zhoutemperature ramped from 65 °C to 95 °C at an increment rate of 0.5 °C/s. Data analysis We used the Bio-Rad CFX Maestro software (version 4.1) to obtain the raw quantification cycle (Cq) values for each reaction Table S2. The relative

(13) Astaxanthin suppresses hepatocellular carcinoma via targeting Wnt/Β-catenin pathway: Experimental study on chemically induced HCC in ratsSci RepApril 21, 2026Mona A. Kortam, Marwa Sh. Ismail, Maher A. Kamel, Noha A. El-Boghdadyannealing at 55 °C for 20 s, and extension at 72 °C for 15 s74. Data acquisition was performed using CFX Maestro™ Software (Bio-Rad, USA). The relative expression levels of cyclin D1, c-Myc and MDR1 were quantified in relation

(14) Acute exposure to cell-free mitochondrial DNA induces pregnancy-specific aortic endothelial dysfunction and organ-selective inflammation in ratsbioRxivApril 19, 2026Nataliia Hula, Reneé de Nazaré Oliveira de Nazaré Oliveira da Silva, Desirae Escalera, Leslie Lopez, Gabrielle Kelly, Isabelle K. Gorham, Megan Rowe, Taiming Liu, Arlin B Blood, Eugenia Mata-Greenwood, et al.described (19) using iQ SYBR Green Supermix (Bio-Rad) on a CFX96 Real-Time PCR Detection System with CFX Maestro Software v.2.3 (Bio-Rad). Cycling conditions consisted of an initial denaturation at 95°C for 3 min, followed

(15) Identification and detection of genetic markers associated with antimicrobial susceptibility and evaluation of efflux pump mechanisms in Mycoplasma iowaePLoS OneApril 17, 2026Dominika Buni, Áron Botond Kovács, Enikő Wehmann, Dénes Grózner, Krisztián Bányai, Eszter Zsófia Nagy, Janet Bradbury, Marco Bottinelli, Elisabetta Stefani, Salvatore Catania, et al.of 12 μl. The melt MAMA assay was optimized for the Bio-Rad CFX96 real-time PCR system using Bio-Rad CFX Maestro software version 1.1 (Bio-Rad Laboratories, Hercules, CA, USA). Thermocycling parameters were 95 °C for 1 min,

(16) Central Administration of Recombinant IGF1 Is Neuroprotective in a Rodent Model of Acute Liver FailureInt J Mol SciApril 16, 2026Yubo Wang, Matthew McMillin, Gabriel Frampton, Kathryn Rhodes, Elaina Williams, Juliet Venter, Jace Tyson, Esha Gupta, Mihika Patankar, Patrick Mireles, et al.factor-α (TNF-α), and GAPDH (Table 1). Relative gene expression was calculated using the ΔΔCt method with CFX Maestro software (version 2.3; Bio-Rad Laboratories, Hercules, CA, USA), using control cortical tissues as reference

(17) TH5487 specifically targets NLRP3 in FCAS patients resistant to MCC950Commun BiolApril 16, 2026Angela Lackner, Sofia I. Picucci, Wenjin Jiang, Janset Onyuru, Melissa Campos, Julia E. Cabral, Lemuel Leonidas, Alijah Macapagal, Hannah Lee, Valerie Henriquez, et al.from 65 °C to 95 °C with 0.5 °C steps and 5 s per step. The readout was visualized using the BioRad CFX Maestro Software. The relative amounts of mRNA of either NLRP3 or ASC were calculated using GAPDH as a control. The final

(18) Probing the Cancer Mutational Landscape of KMT2 Regulatory SubunitsFASEB JApril 15, 2026Sabrina Grégoire, Sara Chow, Monika Joshi, Pamela Zhang, Aws Almir Ahmad, Ashley Janna, Veronique Tremblay, Marcelo Muñoz, Arvind Mer, Jean-Francois Couturefrom 10°C to 95°C at a heating rate of 0.5°C per cycle, each lasting 1 s. Data were analyzed using CFX Maestro Software, and the second derivative of the fluorescence signal was used to determine the melting temperature

(19) Caspase-6 Controls Lipid and Energy Metabolism in Diet-Induced ObesityAdv Sci (Weinh)April 13, 2026Abhishek Gupta, Wenjing You, Linmeng Han, Jianfei Ji, Meixia Pan, Xianlin Han, Xiaoli Sun, Peng Zhaorecorded using the CFX Opus 384 Real‐Time PCR System (Bio‐Rad Laboratories) and analyzed with Bio‐Rad CFX Maestro software. Primer sequences used for gene expression analysis are as follows: Table 1. QPCR primer sequences 4.9

(20) Role of AtCPK5 and AtCPK6 in the regulation of the plant immune response triggered by rhamnolipids in ArabidopsisPLoS OneApril 13, 2026Juliette Stanek, Olivier Fernandez, Marie Boudsocq, Dina Aggad, Sandra Villaume, Laetitia Parent, Sandrine Dhondt-Cordelier, Jérôme Crouzet, Stéphan Dorey, Sylvain Cordeliertreated and untreated with RLs was assessed. The M-Score calculation was performed using the BioRad CFX Maestro software to select the three most stable genes under our experimental conditions. AtActin7 (GenBank NM_121018.4;

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