Precision Melt Analysis Software from Bio-Rad

Product Specs
ItemPrecision Melt Analysis Software
CompanyBio-Rad
Price $5,343.00Supplier Page View Company Product Page
Catalog Number1845025
SystemMinimum: Windows 7
Recommended: Windows 10

Specifications/Features

Operating system: Minimum: Windows 7
Recommended: Windows 10

Software: Microsoft Office Suite

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Multiplex high resolution melting using MADC2 and SCAR markers for sex identification of Cannabis sativa L. cultivationSci RepApril 1, 2026Maturada Siengthong, Puntitra Kamol, Apitssamai Yodkham, Phuping Sucharitakul, Siriwadee Chomdej, Kawee Sujipuli, Kittisak Buddhachatfor males and females were analyzed to identify appropriate DNA markers for sex determination through Precision Melt Analysis™ Software 1.3 (Bio-Rad, USA). Two key criteria were applied to choose suitable primers: (i) distinct differences

(2) Genetic and functional characterization of AMH Signaling in Zebrafish - Evidence for Roles of Amh-Bmpr2a-Bmpr1bb Pathway in Controlling Gonadal HomeostasisPLOS GeneticsMarch 23, 2026Yiming Yue, Chu Zeng, Xin Zhang, Chao Bian, Zhiwei Zhang, Kun Wu, Weiting Chen, Xianqing Zhou, Ling Lu, Nana Ai, et al.wild-type and mutant alleles based on their distinct melting profiles. The data was analyzed using Precision Melt Analysis software (Bio-Rad). The primers used for HRMA are listed in S1 Table. Genotyping by heteroduplex motility assay

(3) Multiplex real-time PCR with high-resolution melting analysis for rapid identification of carbapenem and colistin resistance genes in clinical Enterobacterales isolatesScientific ReportsMarch 3, 2026Sirirat Luk-In, Kamonrat Phopin, Sasina Bangmuangngam, Tanittha Chatsuwan, Dhammika Leshan Wannigama, Aye Mya Sithu Shein, Ratana Lawung, Tanawut Tantimongcolwatsoftware-assisted analysis and a robust positive-control framework to achieve optimal diagnostic accuracy. We used Precision Melt Analysis™ software version 1.2 (Bio-Rad Laboratories, Inc., USA) to normalise and cluster the melt curves, and then difference

(4) Rapid Authentication of Flowers of Panax ginseng and Panax notoginseng Using High-Resolution Melting (HRM) AnalysisMoleculesJanuary 27, 2026Menghu Wang, Wenpei Li, Yafeng Zuo, Qianqian Jiang, Jincai Li, Wenhai Zhang, Xiangsong MengBio-Rad CFX Connect Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA), integrated with Precision Melt Analysis™ software (v3.1). To ensure thermal uniformity and optimal fluorescence sensitivity, the instrument was pre-calibrated

(5) High-Resolution Melting Analysis for Differentiation of Sclerotium (= Agroathelia ) rolfsii , S. delphinii , and Two Intraspecific Groups of S. rolfsiiMycobiologyNovember 2, 2025Dong Jae Lee, Young-Joon ChoiFluorescence data were automatically acquired on the HRM channel, and all data were analyzed using the Precision Melt Analysis software (Bio-Rad, Hercules, CA). 2.6. Specificity and sensitivity Specificity of the HRM primers was tested

(6) DKK2 contributes to context discrimination and adult hippocampal neurogenesis by suppressing Wnt/PCP signalingNeuropsychopharmacologyOctober 22, 2025Woo Seok Song, Hyochul Lee, Jae Min Lim, Sang Ho Yoon, Young Sook Kim, Hyeonjin Kim, Myoung-Hwan Kimannealing/extension at the primer-specific melting temperature for 1 min. Melting curve analysis was conducted using Precision Melt Analysis Software (Bio-Rad). GAPDH was used as a reference gene. Primer specificity and efficiency were validated by RT-PCR

(7) FCRLs and atypical transcriptional pattern in tumor infiltrating B cells from lung and renal cancerFrontiers in ImmunologySeptember 8, 2025Ekaterina A. Bryushkova, Evgenii V. Shchoka, Daniil K. Lukyanov, Marina B. Gikalo, Natalya Mushenkova, Konstantin K. Laktionov, Alexey M. Kazakov, Oybek A. Khalmurzaev, Vsevolod B. Matveev, Dmitriy M. Chudakov, et al.CFX96 Touch Real-Time PCR Detection System (BioRad) equipped with CFX Manager™ Software (BioRad) and Precision Melt Analysis™ Software (BioRad) were used to ensure that the primers amplified the desired amplicons without forming dimers,

(8) Frequencies and subtypes of glycophorin GYP(B-A-B) hybrids among northern Thais, Burmese, and Karen with a previous history of malaria infection: a study in the Thailand-Myanmar border areaPeerJJune 23, 2025Pornsawan Srichankhot, Arissara Nakapong, Anocha Sukhanpob, Panadda Chapandoong, Amonrat Jumnainsong, Chanvit Leelayuwat, Piyapong Simtongdecrease in fluorescence intensity. The fluorescence change rate was monitored and analyzed using Bio-Rad Precision Melt Analysis software (Bio-Rad Laboratories Inc.). The melting curve profiles of unknown samples were compared to the DNA

(9) Unraveling the Role of Ctla-4 in Intestinal Immune Homeostasis: Insights from a novel Zebrafish Model of Inflammatory Bowel DiseaseElifeMay 20, 2025Lulu Qin, Chongbin Hu, Qiong Zhao, Yong Wang, Dongdong Fan, Aifu Lin, Lixin Xiang, Ye Chen, Jianzhong Shaoquantified using quantitative real-time PCR on a CFX Connect Real-Time PCR Detection System equipped with Precision Melt Analysis Software (Bio-Rad, Cat. No. 1855200EM1). Total RNA from intestines was extracted using TRIzol reagent (Takara

(10) Metabolic Reprogramming in HIV+ CD4+ T-Cells: Implications for Immune Dysfunction and Therapeutic Targets in M. tuberculosis Co-InfectionMetabolitesApril 22, 2025Suheena Ayrga, Gerrit KoorsenDNA standards to quantify methylation levels. qPCR was performed, and melt curves were analysed using Precision Melt Analysis™ software (v 4.0.52.0602, Bio-Rad, Johannesburg, South Africa). Statistical analysis involved non-parametric tests,

(11) Vitellogenin receptor mediates heat adaptability of oocyte development in mud crabs and zebrafishNat CommunApril 19, 2025Long Zhang, Kun Wu, Haoyang Li, Minwen Zhan, Sheng Wang, Enhui Song, Qi Zhang, Jian He, Xinyi He, Menghuang Xu, et al.for neutralization. The extract was used for HRMA assay, and the melt curves were analyzed with the Precision Melt Analysis software (Bio-Rad, Hercules, CA). The primers used for genotyping were listed in Supplementary Table 15. Sampling

(12) The Clinical and Laboratory Landscape of COVID-19 During the Initial Period of the Pandemic and at the Beginning of the Omicron EraVirusesMarch 27, 2025Yulia A. Desheva, Tamara N. Shvedova, Olga S. Kopteva, Danila S. Guzenkov, Polina A. Kudar, Tatiana S. Kotomina, Daria S. Petrachkova, Elena P. Grigorieva, Anna A. Lerner, Stanislav V. PonkratovSsoFastEvaGreen mixtures on a CF X96 (Biorad, Hercules, CA, USA) device. Melting curves were analyzed using the Precision Melt analysis software (version 1.3) as described previously [28]. 2.4. Laboratory Data Serum C-reactive protein (CRP) and

(13) Disrupting Amh and androgen signaling reveals their distinct roles in zebrafish gonadal differentiation and gametogenesisCommun BiolMarch 5, 2025Kun Wu, Yiming Yue, Lingling Zhou, Zhiwei Zhang, Hong Shan, Huanhuan He, Wei Geneutralization. The DNA extract was then used for HRMA, and the melt curves were analyzed using the Precision Melt Analysis software (Bio-Rad, Hercules, CA) according to our previous studies51. Primers used for genotyping are listed

(14) Rapid Detection of Epinephelus Species Substitution in the Greek Market Using High-Resolution Melting AnalysisGenes (Basel)February 22, 2025Evanthia Chatzoglou, Nefeli Tsaousi, Ariadni Spetsieri, Emmanouil E. Malandrakis, Helen Miliouincrements from 65 °C to 95 °C as previously described [24]. Curve data analysis was performed with Bio-Rad Precision Melt Analysis Software, version 1.2 (Bio-Rad Laboratories Inc. Hercules, CA, USA) [47]. To enhance the accuracy of identifying

(15) Differential expression of CCD4(4B) drives natural variation in fruit carotenoid content in strawberry (Fragaria spp.)Plant Biotechnology JournalJanuary 22, 2025Iraida Amaya, F. Javier Roldán‐Guerra, José L. Ordóñez‐Díaz, Rocío Torreblanca, Henning Wagner, Veronika Waurich, Klaus Olbricht, José M. Moreno‐Rojas, José F. Sánchez‐Sevilla, Cristina Castillejofor annealing/extension and 45 cycles of amplification. HRM variants were analysed with the Bio‐Rad Precision Melt Analysis™ Software. Statistical analysis GraphPad Prism 9.4.1 was used for statistical analysis. Pairwise comparisons

(16) Association of Single-Nucleotide Variants in ACE2 with the Persistence of Positive qPCR Test for SARS-CoV-2 in Healthcare Professionals During the First Wave of the COVID-19 PandemicMicroorganismsDecember 12, 2024Karina Jiménez-Gil, Jorge Alberto Cerón-Albarrán, Melissa Daniella Gonzalez-Fernandez, Rosalba Sevilla-Montoya, Alberto Hidalgo-Bravo, Javier Angeles-Martínez, Daniel Montes-Herrera, Oscar Villavicencio-Carrisoza, Carmen Selene García-Romero, José Esteban Muñoz-Medina, et al.standardized, and all samples were analyzed in triplicate. Melting curve analysis was performed with Bio-Rad Precision Melt Analysis Software v.1.3 (Hercules, CA, USA). Samples displaying melting curve deviations from the average (the most frequently

(17) Engineering an fgfr4 knockout zebrafish to study its role in development and diseasePLOS ONENovember 22, 2024Emma N. Harrison, Amanda N. Jay, Matthew R. Kent, Talia P. Sukienik, Collette A. LaVigne, Genevieve C. Kendallcycles; 65°C for 30 s; melt 65°C–95°C, 0.2°C/step hold 5 s; 95°C for 15 s. Bio-Rad Maestro and Bio-Rad Precision Melt Analysis software were used for data analysis, with known wildtype samples as the reference group. A-tail cloning To

(18) High-Resolution Melting Analysis Potential for Saccharomyces cerevisiae var. boulardii Authentication in Probiotic-Enriched Food MatricesBioTech (Basel)November 14, 2024Monika Borkowska, Michał Kułakowski, Kamila Myszkareagents, No Template Control (NTC) for each primer pair was run in parallel. 2.5.3. HRM Analysis Precision Melt Analysis Software (PMAS) (Bio-Rad Laboratories, Inc.) was used to analyze the generated qPCR amplicons. The pre- and post-melt

(19) Detection and molecular characterization of lumpy skin disease and bovine papular stomatitis viruses in lumpy skin disease-suspected outbreaks in TanzaniaVirology JournalNovember 5, 2024Fredy T. Makoga, Jelly S. Chang’a, Irene K. Meki, Charles Mayenga, Tirumala B. K. Settypalli, Stella Bitanyi, Bishop Magidanga, Emma Peter, Augustino Chengula, Giovanni Cattoli, et al.were analyzed on Bio-Rad CFX Maestro™ Software version 1.1, and the HRM profiles analyzed using the Precision Melt Analysis™ Software version 1.2 (Bio-Rad, Hercules, CA, USA). Targeted gene sequencing and characterization of Tanzanian

(20) Assessing pyrethroid resistance in Aedes aegypti from Cordoba Colombia: Implications of kdr mutationsPLOS ONEAugust 22, 2024María Claudia Atencia–Pineda, Diana Diaz-Ortiz, Paula Pareja–Loaiza, Javier García–Leal, Richard Hoyos–López, Alfonso Calderón–Rangel, Pedro Fragozo-Castilla, Lisandro Pacheco-Lugo, Adriana E. Flores, Ronald Maestre–Serranoof 0.2°C every 10 s. The interpretation of the calibration curves was carried out with the Bio-Rad Precision Melt Analysis software as follows: for the V1016I mutation, a peak at 79 °C corresponds to a homozygous mutant (I/I), at 86

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