T7 RiboMAX Express RNAi System from Promega

Product Specs
ItemT7 RiboMAX Express RNAi System
CompanyPromega
Price $335.00Supplier Page View Company Product Page
Catalog NumberP1700
Quantity50 x 20µl reactions
PromoterT7

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cellseLifeMarch 25, 2026Qi Zhang, Yunfeng Wang, Xueli Fu, Ziguang Wang, Yang Zhang, Lizhong Yan, Yuejia Wang, Muhan Yang, Dongze Song, Ruixing Zhang, et al.kit,ACCURATE BIOLOGY,Cat# AG11701-S, Commercial assay or kit,T7 RiboMAX Express RNAi System,Promega,Cat# P1700, Commercial assay or kit,TriQuick Reagent kit,Solarbio,Cat# R1100, "Chemical compound, drug",Chloroquine

(2) Thermal Plasticity is Regulated by a Key MicroRNA During Range Expansion of an Invasive Fruit FlyAdvanced ScienceFebruary 24, 2026Yan Zhao, Juntao Hu, Juan Du, Shaokun Guo, Youming Hou, Zhihong LiFor RNAi, dsRNA (dsthw and dsGFP) was synthesized in vitro using the T7 RiboMAX Express RNAi System (P1700, Promega, USA). Primers for dsRNA synthesis are listed in Table S7. Adults were injected with 1 µg of

(3) Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogrammingNature CommunicationsFebruary 2, 2026Jiaming Liu, Yashuai Wu, Zhenfang Li, Junbo Tang, Xin Zhou, Shiqi LuoSupplementary Table 2. The dsRNA was synthesized using the T7 RiboMAX™ Express RNAi System Kit (Cat#P1700, Promega, USA) and T7 promoter-containing primers. The synthesized dsRNA was mixed with star polycation

(4) A Cold Stress‐Activated Endocrine Sentinel Chemical Hormone Promotes Insect Survival via Mitochondrial Adaptations Through the Adipokinetic Hormone ReceptorAdvanced ScienceFebruary 1, 2026Jiao Zhou, Junxian Chen, Hongxia Zhang, Lu Guo, Kevin Mai, Elijah Abraham, Dilip V. Prajapati, Yue Chang, Jing Ning, Xinchen Wang, et al.manufacturer's instructions. 4.8 RNAi Experiments dsRNA was prepared using a T7 RNAi Transcription Kit (P1700, Promega) following the manufacturer's instructions. Using a Hamilton Glass Syringes (1700 Series microliter

(5) The insect Toll pathway activates antibacterial immunity against the citrus Huanglongbing pathogenNature CommunicationsJanuary 23, 2026Yu Du, Mengle Sun, Yuqing Xiao, Jinhua Yang, Manman Hu, Qian Chen, You Li, Taiyun WeiIKKE, NFAT, Reeler, NOS, and GFP were synthesized using the T7 RiboMAX Express RNAi system (Promega, P1700). Approximately 600 CLas-free or CLas-positive adults (8 days padp) were microinjected with 46 nL dsRNAs

(6) A secreted C-type lectin in Periplaneta americana functioning in antibacterial activity, innate immune signaling and leg regenerationFrontiers in ImmunologyJanuary 20, 2026Xiaoxuan Liu, Nan Sun, Shuang Geng, Shuqi Xian, Xiaojuan Wu, Ying Huang, Yechun Peitreatment Double-stranded RNA (dsRNA) was synthesized using the T7 RiboMAX Express RNA interference (RNAi) System (Promega, P1700). Following purification, dsRNA was prepared at a concentration of 2 μg/μL, and 2μg was microinjected

(7) Plant Virus‐Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental TransmissionAdvanced ScienceJanuary 1, 2026Haibo Wu, Wenqiang Wan, Qingquan Liang, Hengsong Yang, Chengcong Lu, Taiyun Wei, Qian Chenregions of ≈1200, 750, and 475 bp, respectively. Subsequently, a T7 RiboMAX Express RNAi System (Promega, P1700) was used for in vitro synthesis of dsRNAs targeting caspase‐8 (dscaspase‐8), IAP (dsIAP), and GFP (dsGFP). At

(8) Unveiling the conserved mechanism of microsporidian vertical transmission: A comparative study of nosema infection across Host speciesVirulenceDecember 24, 2025Chunxia Wang, Yongzhi Kong, Zishen Tang, Tongyu Luo, Xuanang Yang, Yongjun Zhang, Tian Li, Zeyang Zhouand green fluorescent protein (GFP) genes were synthesized using the T7 RiboMAX Express RNAi System (P1700, Promega, USA) according to the manufacturer’s instructions. The specific primers used to generate the

(9) High-resolution genetic maps and QTL mapping applications reveal polycystin gene involvement in oyster shell formationiScienceDecember 19, 2025Haitao Ma, Yanping Qin, Dongmei Yu, Bingke Jiao, Qingliang Liao, Yang Zhang, Yinjie Zhang, Jingyue Huang, Jun Li, Ziniu Yu, et al.Synthesis Kit,"TAKARA, Kusatsu, Japan",Cat#6210B T7 RiboMAX™ Express RNAi System,"Promega, USA",Cat#P1700 DIG RNA Labeling Kit (SP6/T7),"Roche, Basel, Switzerland",Cat#11175025910 pMD19-T Vector Cloning Kit,"TAKARA,

(10) Polo-like kinase 1 regulates growth in juvenile Fasciola hepaticaPLoS PathogDecember 2, 2025Paul McCusker, Nathan G. Clarke, Rebecca Armstrong, Duncan Wells, Emily Robb, Paul McVeigh, Andreas Krasky, John Harrington, Paul M. Selzer, Nikki J. Marks, et al.(#CS12000, Thermo Fisher Scientific) and dsRNA generation using the T7 RiboMAX Express RNA System (#P1700, Promega). All dsRNAs were resuspended in ddH2O with concentrations/purities checked on a DeNovix DS-11 FX

(11) Temporal changes in amino acid profiles coordinate mosquito physiology and egg maturation in Aedes aegyptiBMC BiologyNovember 27, 2025Yusuke Kato, Ayano Oi, Naoshi Dohmae, Fumiaki Obata, Chisako Sakumaextraction (FG-91302, NIPPON Genetics). dsRNA was synthesized using the T7 RiboMAX™ Express RNAi System (P1700, Promega). Briefly, purified PCR products were used as templates for in vitro transcription with T7 RNA polymerase

(12) Insect I‐Type Lysozymes Function as Antiviral Proteases by Forming Biomolecular CondensatesAdvanced ScienceOctober 27, 2025Yu Du, Yuqing Xiao, Manman Hu, Jinhua Yang, You Li, Taiyun WeiRdToll1, RdMyD88, RdDorsal, and GFP were synthesized using the T7 RiboMAX Express RNAi system (Promega, P1700). The primers used for dsRNA synthesis are listed in Table S1 of the Supporting Information. Approximately

(13) Reoviruses hijack the SMARCB1-MYC transcriptional regulation complex to activate autophagy for persistent viral infection in leafhopper vectorPLoS PathogOctober 9, 2025Hui Wang, Runfa Liu, Guangming Xiao, Yanan Li, Bozhong Li, Qian Chen, Taiyun Wei(dsGFP) were synthesized according to the protocol for the T7 RiboMAX Express RNAi System kit (Promega, P1700). To test the suppression of NcSMARCB1 or NcMYC expression on RDV infection in N. cincticeps, viruliferous

(14) Systemic identification and characterization of the conserved core NuRD complex in planarianFrontiers in AgingSeptember 30, 2025Lei Huang, Hao Wang, Shuang Wu, Jiangnan Chai, Xiaopeng Zou, Hongfei Liu, Zhengwei Guo, Yanming Wang, Yunchao KanRNA interference All the dsRNAs were synthesized using an in vitro transcription reaction kit (Promega, P1700) from PCR-generated DNA templates with flanking T7 promoters, ethanol precipitated, and annealed after

(15) Locusts adopt IP 3 as a second messenger for olfactory signal transductionScience AdvancesSeptember 12, 2025Jing Yang, Helen He, Shijie Dong, Jing Lv, Lili Cheng, Qiaoqiao Yu, Le Kang, Xiaojiao Guoin this assay are listed in table S3. RNAi assay The T7 RiboMAX Express RNAi system (Promega, WI, P1700) was used to synthesize dsRNA molecules targeting the green fluorescent protein (GFP), Clvs2, OBP13,

(16) Comparative Transcriptome Analysis Reveals Epithelial Growth Factor Receptor (EGFR) Pathway and Secreted C-Type Lectins as Essential Drivers of Leg Regeneration in Periplaneta americanaInsectsSeptember 5, 2025Xiaoxuan Liu, Nan Sun, Xiaojuan Wu, Jiajia Wu, Shuqi Xian, Dayong Wang, Yechun Peiwas synthesized using a T7 RiboMAX Express RNA interference (RNAi) System (Promega, Madison, WI, USA, P1700). Following purification, a dsRNA solution of 2 μg/μL was microinjected into the coxa of each nymph,

(17) Comprehensive characterization of the impairing effects of Nosema bombycis on the host digestive integrity and functionmSphereAugust 26, 2025Lu Cao, Siying Yin, Dexu Liu, Yunlin Tang, Wenxin Yang, Maoshuang Ran, Jie Chen, Guoqing Pan, Zeyang Zhou, Jialing Baoprotein (EGFP) genes were synthesized by specific primers (Table S1). The T7 RiboMAX Express RNAi System (P1700, Promega, USA) was applied according to the manufacturer’s instructions. Purified dsRNAs were assessed

(18) Transcriptome-wide regulation of folate on neural mRNA m6A methylome via carbon metabolism in Drosophila and mammalsCommun BiolAugust 4, 2025Anrui Liu, Zhengyi Huo, Zihe Xu, Sisi Liu, Yuting Jiao, Rui Li, Yi Lu, Meng Yang, Jia Huang, Chen Huang, et al.shown in Supplementary Table S2. RNAi using dsRNA in S2 cells The T7 RiboMAX™ Express RNAi System (#P1700, Promega, WI, USA) was used to synthesize dsRNA molecules following the manufacturer’s protocol. The

(19) Lipid metabolites affected by deficient autophagy antagonize the occurrence of autophagy through AMPK signaling in insectsBMC BiolJuly 1, 2025Ling Tian, Qien Zhong, Yubei Yang, Wenmei Wu, Yang Xiao, Sheng Li, Kang LiEgfp, BmAtg1, BmAtg5, and BmAtg8 was generated using the T7 RiboMAX™ Express RNAi system (Promega, P1700) according to the manufacturer’s instructions. Each individual larva was injected with 30 μg dsRNA at

(20) Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae)STAR ProtocolsJune 20, 2025Lourdes E. Córdoba, Alicia R. Pérez de Rosas, Beatriz A. García, María M. StroppaPlatinum Taq DNA Polymerase,Invitrogen,Cat#10966018 QIAquick PCR Purification Kit,QIAGEN,Cat#28104 T7 RiboMAX Express RNAi System,Promega,Cat#P1700 Qubit RNA HS Assay Kit,Invitrogen,Cat#Q32852 SensiFAST SYBR Lo-ROX One-Step Kit,Bioline,Cat#BIO-74005 "HOT

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