ET SSB from New England Biolabs

Product Specs
ItemET SSB
CompanyNew England Biolabs
Price $176.00 Inquire
Catalog NumberM2401S
Quantity50 µg
ApplicationsDNA Manipulation
TypeProteins
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The MIRO1-BAX Complex Dictates Life and Death at the Mitochondrial GatebioRxivJuly 1, 2026Alva G. Sainz, Chulhwan S. Kwak, Kwang Bog Cho, Sejal A. Sripadanna, Brandon H. Bergsneider, Zoe Zizzo, Aarooran S. Durairaj, Zehui Du, Ian Cooney, Anthony Venida, et al.Phusion polymerase (M0530S, NEB); 50 μL 40 U/μL Taq ligase (M0208L, NEB); 3.1 μL 500 μg/mL ET SSB (M2401S, NEB); 215.2 μL millipore water. For each Gibson assembly reaction, an equal volume of 2× Gibson Assembly Master Mix

(2) Genetic Diversity and Population Structure of the Black-Footed Cat: Insights into Felis's Deadliest PredatorbioRxivJune 2, 2026Victoria Belle Grant, Kelsie Hunnicutt, Michelle Schroeder, Martina Küsters, Jonas Oppenheimer, Shreya Banerjee, John J. Baczenas, Dmitri Petrov, Jacqueline M. Bishop, Nadine Lamberski, et al.designed for ancient DNA extractions32,33. We combined 10 μL of DNA extract with 1μL of 76 ng/μL SSB (NEB, M2401S) in PCR tubes. We denatured input DNA in the thermocycler at 95°C for 3 minutes with a lid temperature

(3) Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactionsNucleic Acids ResFebruary 16, 2026James M Robson, Alexander A Green(New England Biolabs M0530S), 4 U/µl Taq ligase (New England Biolabs M0208L), and 7 ng/µl ET SSB (New England Biolabs M2401S) in Gibson buffer [1 mM dNTPs (New England Biolabs N0446S), 5 mM NAD+ (New England Biolabs B9007S),

(4) Toehold-VISTA: A machine learning approach to decipher programmable RNA sensor-target interactionsbioRxivAugust 13, 2025James M. Robson, Alexander A. Green(New England Biolabs M0530S), 4 U/μL Taq ligase (New England Biolabs M0208L), and 7 ng/μL ET SSB (New England Biolabs M2401S) in Gibson buffer [1 mM dNTPs (New England Biolabs N0446S), 5 mM NAD+ (New England Biolabs B9007S),

(5) Long-read RNA sequencing of transposable elements from single cells using CELLO-seqNature ProtocolsJuly 16, 2025Sophie A. Marlow, Lauryn A. Deaville, Rebecca V. BerrensPCR Kit (Roche: KK2802)-Betaine solution (5M; Sigma-Aldrich: B0300-5vl)-ET SSB (New England Biolabs: M2401S)CRITICAL: we are not aware of a substitute item, but the ET SSB leads to an increase in cDNA length

(6) Wide-spectrum profiling of plasma cell-free RNA and the potential for health-monitoringRNA BiologyMarch 20, 2025Xinxin Wang, Shaogang Li, Rijing Ou, Wending Pang, Yingying Wang, Yifan Zhang, Yu Lin, Changlin Yang, Wei Chen, Changgui Lei, et al.LS-EZ-E-000150), 2 μL Tris (PH 8.0) (SIGMA, T2694–100 ML, 440 mm each), 0.6 μL ET SSB (New England Biolabs, M2401S), 2 μL USER™ Enzyme (New England Biolabs, M5505L) and 20.4 μL DEPC water (BBI, D1005–500) were added

(7) Atlas-scale single-cell DNA methylation profiling with sciMETv3Cell GenomicsJanuary 8, 2025Ruth V. Nichols, Lauren E. Rylaarsdam, Brendan L. O’Connell, Zohar Shipony, Nika Iremadze, Sonia N. Acharya, Andrew C. Adey,New England Biolabs,M0202M T4 Polynucleotide Kinase,New England Biolabs,M0201S ET-SSB,New England Biolabs,M2401S T4 RNA Ligase Buffer,New England Biolabs,B0216L PEG 8000 (50% w/v),New England Biolabs,B0216L "1,3-Propanediol",Merck,504-63-2 ATP

(8) Fluorescence-activated droplet sequencing (FAD-seq) directly provides sequences of screening hits in antibody discoveryProceedings of the National Academy of SciencesSeptember 10, 2024Alexis Autour, Christoph A. MertenM0314), 6 U/µL RT Maxima (Thermo Fisher Scientific, Cat. No. EP0742), 22.5 ng/µL ET SSB (NEB, Cat. No. M2401S), 0.15 U/µL Q5 HotStart DNA polymerase (NEB, Cat. No. M0493), and primer mix (SI Appendix, Table S5).

(9) Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of InterestAdvanced ScienceAugust 1, 2024Xiong Ding, Yaru Wang, Yuxin Gui, Chuankun Yang(Cat# N0447L), Bst 2.0 WarmStar DNA polymerase (Cat# M0538L), WarmStart RTx (Cat# M0380S), ET SSB (Cat# M2401S), and nuclease‐free water (Cat# B1500S) were purchased from New England Biolabs (NEB, USA). RNase H

(10) IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolutionSTAR ProtocolsDecember 1, 2023Monique A.J. van Eijndhoven, Chantal Scheepbouwer, Ernesto Aparicio-Puerta, Michael Hackenberg, D. Michiel Pegtelsingle-stranded DNA-specific exonuclease (RecJf) for 1 h at 30°C (all enzymes from New England Biolabs, Cat#M2401S, M0331S, M0264S). However, in the gel size selection process and after sequencing we did not observe

(11) H4K20me3 is important for Ash1-mediated H3K36me3 and transcriptional silencing in facultative heterochromatin in a fungal pathogenPLOS GeneticsSeptember 25, 2023Mareike Möller, John B. Ridenour, Devin F. Wright, Faith A. Martin, Michael Freitag#M0363], 6.25 μL Phusion DNA-Polymerase [2 U/μL ThermoFisher #F530L], 3.125 μL ET SSB [500 μg/ml NEB #M2401S], 50 μL Taq DNA-Ligase [40 U/μL stock NEB #M0208L]). Primers, plasmids, and strains used in this study

(12) Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencingNucleic acids researchAugust 25, 2023Chen YC, Chen CY, Chiang TW, Chan MH, Hsiao M, Ke HM, Tsai IJ, Chuang TJ.buffer (1 μl), RNaseOut (0.5 μl; Thermo Fisher Scientific, Cat. 107770179), and ET SSB (0.2 μl; NEB, M2401S). Hybridization was accomplished by initially subjecting the sample to thermal treatment at 90°C for

(13) A rapid cell-free expression and screening platform for antibody discoveryNature communicationsJuly 3, 2023Hunt AC, Vögeli B, Hassan AO, Guerrero L, Kightlinger W, Yoesep DJ, Krüger A, DeWinter M, Diamond MS, Karim AS, et al.backbone following the published protocol with the addition of 3.125 μg/mL of ET SSB (NEB, product no. M2401S)83,84. 20 ng of purified, linear pJL1 backbone, 20 ng of purified, linear sdFab VH or VL constant DNA,

(14) Locus-specific expression of transposable elements in single cells with CELLO-seqNature BiotechnologyApril 1, 2022Rebecca V. Berrens, Andrian Yang, Christopher E. Laumer, Aaron T. L. Lun, Florian Bieberich, Cheuk-Ting Law, Guocheng Lan, Maria Imaz, Joseph S. Bowness, Neil Brockdorff, et al.5M betaine, 0.1 μl of (100μM) template switch oligo (IDT), 0.5 μl of 100mM DTT, 0.1 μl ET SSB (NEB #M2401S) and 1.6 μl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049).

(15) An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 proteinPLOS ONEMarch 24, 2022Yi Heng Nai, Egan H. Doeven, Rosanne M. Guijtcombination with the LEM cocktail at 2 μg of RecA (M0249, New England Biolabs (NEB)), 80 ng ET SSB (M2401S, NEB) or gp32 (M0300, NEB) at a concentration range between 50–320 ng/μL per reaction respectively.

(16) CD20 as a gatekeeper of the resting state of human B cellsProceedings of the National Academy of Sciences of the United States of AmericaFebruary 16, 2021Kläsener K, Jellusova J, Andrieux G, Salzer U, Böhler C, Steiner SN, Albinus JB, Cavallari M, Süß B, Voll RE, et al.templates were mixed at fourfold molar excess with a single-strand DNA binding protein (ET SSB; NEB; M2401S) and heated at 95 °C for 10 min to achieve single-stranded donor oligonucleotides bound to ET SSB. Two

(17) A simple bead-based method for generating cost-effective co-barcoded sequence readsProtocol ExchangeOctober 11, 2018Brock Peters et al.EDTA \(0.5 M, pH 8.0) \(Sigma-Aldrich, cat. no. 03690100ML) ET SSB \(500 μg/ml) \(New England Biolabs, cat. no. M2401S) Exonuclease I \(20,000 units/mL) \(New England Biolabs, cat. no. M0293L) Exonuclease III \(100,000

(18) Translation complex profile sequencing to study the in vivo dynamics of mRNA–ribosome interactions during translation initiation, elongation and terminationNature ProtocolsApril 1, 2017Nikolay E Shirokikh, Stuart K Archer, Traude H Beilharz, David Powell, Thomas Preiss(extreme thermostable single-stranded DNA-binding protein), 0.5 μg/μl (New England BioLabs, cat. no. M2401S) • GRGreen DNA Loading Buffer, 6× (Excellgen, cat. no. EG-1008) • 100-bp DNA Ladder, 0.5 μg/μl (New England BioLabs

(19) Probe‐Directed Degradation (PDD) for Flexible Removal of Unwanted cDNA Sequences from RNA‐Seq LibrariesCurrent Protocols in Human GeneticsApril 1, 2015Stuart K. Archer, Nikolay E. Shirokikh, Thomas Preisswater) 0.5 μg/μl Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB protein; NEB, cat. no. M2401S) 2.5 U/μl Platinum Pfx DNA Polymerase (Invitrogen/Life Technologies, cat. no. 11708-013) UltraPure Agarose

(20) Normalizing cDNA LibrariesCurrent Protocols in Molecular BiologyApril 1, 2010Ekaterina A. , Irina , Ekaterina V. , Ilya , Dmitry A. , Sergey A.

Be the First to Write a Review!

ET SSB

ET SSB from New England Biolabs

Description

New England Biolabs

Citations (20)

Reviews

ET SSB from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map