Description
GUIDE-seq™ is a cell-based, orthogonal nomination assay that enables genome-wide, unbiased identification of nuclease-cleaved double-stranded breaks at on- and off-target sites. GUIDE-seq uses cotransfection of a dsODN tag with the gene-editing machinery to profile potential off-target effects directly in cells via tag-specific amplification and sequencing. GUIDE-seq has been widely used by academics and industry to support IND filling.