Description
Custom cGMP Oligos produced in our suite with three laboratories: Synthesis, Purification, and Quality Control.
The following applies:
- Manufactured in accordance with US GMP (21 CFR 820)
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Filled in classified cleanroom ISO 14644-1:2015 Class 8
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Site maintains ISO 13485 Quality Management System certificate
Benefits of oligos made under these quality and manufacturing systems include:
- Controlled & validated processes
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Extensive materials traceability
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Batch-to-batch consistency
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Reproducible results
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Bulk amounts.
Quality Control:
- Sequence identity verification by mass spectrometry.
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Quantification by UV spectroscopy.
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Appearance check by inspection.
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Optional Quality Controls:
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Purity verification by analytical HPLC.
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DNase/RNase measurement.
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Endotoxin testing.
Custom cGMP oligonucleotides offer high consistency and quality, suitable for commercialization or assay development across various technologies, including qPCR, isothermal amplification, and NGS.
Custom DNA Oligos:
- Types: Basic PCR primers, SYBR® Green Primers, isothermal amplification primers, sequencing primers.
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Amounts: µg to multi-g
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Purifications: Desalt & HPLC
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Sequence Lengths: 7 – 100 bases
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Modifications: Most commercially available
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Formats: Pooling & aliquoting, dry or in solution, tubes.
Custom qPCR Probes:
- Types: Dual-Labeled Probes, Molecular Beacons, LightCycler® Probes.
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Amounts: µg to multi-g
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Purifications: HPLC
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Sequence Lengths: 7 – 50 bases
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Modifications: Common reporters, BHQ™, MGB:EDQ, Locked Nucleic Acid.
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Formats: Pooling & aliquoting, dry or in solution, tubes.
Next-Gen Sequencing Oligos:
- Types: Custom next-generation sequencing adapters.
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Amounts: g to multi-g
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Purifications: NGSO-Gold (proprietary): <0.25%>
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Sequence Lengths: 10 – 80 bases
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Modifications: 5'-Phosphate, Phosphorothioate, 5-Me-dC.
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Formats: Pooling & aliquoting, dry or in solution, tubes.