MidiMACS™ Starting Kit (LS) from Miltenyi Biotec

(1) Protocol to generate immunocompetent human heart-macrophage assembloids for the study of immune-cardiac interactionsSTAR ProtocMarch 20, 2026Colin O'Hern, Mia Dionise, Sammantha Caywood, Merlinda-Loraine Sewavi, Hussain Basrai, Priyadharshni Muniyandi, Brett Volmert, Aleksandra Kostina, Amanda Huang, Nagib Chalfoun, et al.assays ,, Mycoplasma Kit,MycoFluor Mycoplasma Detection Kit,Cat #M7006 MidiMACS Starting Kit,Miltenyi Biotec,Cat #130-042-301 ZymoPURE II Plasmid Midiprep Kit,Zymo Reseach,Cat #D4200 ,, Experimental models: Cell lines,Experimental models

(2) Lineage specification into GABAergic, glutamatergic, dopaminergic, and astrocytic phenotypes using MUSE stem cells: a novel approach for modeling neurodegenerative and psychiatric disordersMolecular PsychiatryMarch 1, 2026Domenico Aprile, Deanira Patrone, Sura Hilal Ahmed Al Sammarraie, Nicola Alessio, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisimm; Corning, cat. no. CLS294875X25) MidiMACS™ Separator and Starting Kits (Miltenyi biotec, cat. no 130-042-301) MS Columns (Miltenyi biotec, cat. no.130-042-201) Multidish (24 well; Falcon, cat. no. 353047) NanoDrop

(3) NG25 Enhances Anti-Tumor Immunity in KRAS-Mutant Colorectal CancerOncoTargets and TherapyJanuary 8, 2026Qi Xiang, Zhigang Mao, Qizhao Ma, Mi Su, Deng Tang, Siqi Lan, Yufei Tang, Ji Zhang, Yufang WangBuffer (Cat. No. C3702) was obtained from Beyotime (Shanghai, China). MidiMACS™ Starting Kits (Cat. No. 130–042-301), LS Columns (Cat. No. 130–041-306), and the Pan T Cell Isolation Kit II (Cat. No. 130–095-130) were

(4) GABA produced by multiple bone marrow cell types regulates hematopoietic stem and progenitor cellsStem Cell ReportsNovember 11, 2025Cesi Deng, Adedamola Elujoba-Bridenstine, Ai Tang Song, Rylie M. Ceplina, Casey J. Ostheimer, Molly C. Pellitteri Hahn, Cameron O. Scarlett, Sahitya Saka, Xuan Pan, Owen J. Tamplincells using c-Kit microBeads (Miltenyi Biotec, 130-091-224) and a Midi MACS separator (Miltenyi Biotec, 130-042-301). BM, PB, or c-Kit-enriched cells were then stained for 30 min at 4°C with combinations of antibodies

(5) Human heart-macrophage assembloids recapitulate immune-cardiac interactions and enable arrhythmia disease modelingCell Stem CellNovember 6, 2025Colin O’Hern, Sammantha Caywood, Shakhlo Aminova, Artem Kiselev, Brett Volmert, Weiheng Cao, Fei Wang, Mia Dionise, Merlinda-Loriane Sewavi, Milana Skoric, et al.#RS-NS96-SK Mycoplasma Kit,MycoFluor Mycoplasma Detection Kit,Cat #M7006 MidiMACS Starting Kit,Miltenyi Biotec,Cat #130-042-301 ZymoPURE II Plasmid Midiprep Kit,Zymo Reseach,Cat #D4200 Pico Green Kit,, Deposited data,, Human

(6) Multimodal glioma immunotherapy combining TLR9-targeted STAT3 antisense oligodeoxynucleotides with PD1 immune checkpoint blockadeNeuro-OncologyOctober 14, 2025Chia-Yang Hung, Elaine Y Kang, Karol Jacek, Chunsong Yu, Xiaowei Zhang, Yicheng Zhu, Maryam Aftabizadeh, Robyn A Wong, Benham Badie, Piotr Świderski, et al.mechanical dispersion and enzymatic digestion using Multi-Tissue Dissociation Kit-1 (Miltenyi Biotec—#130-042-301). Extracellular staining was performed using fluorochrome-labeled antibodies to mouse CD3 (#145-2C11),

(7) Multi-omic integration with human dorsal root ganglia proteomics highlights TNFα signalling as a relevant sexually dimorphic pathwayPainOctober 1, 2025Allison M. Barry, Julia R. Sondermann, Joseph B. Lesnak, Feng Xian, Úrzula Franco-Enzástiga, Jayden A. O'Brien, David Gomez-Varela, Morgan K. Schackmuth, Stephanie Shiers, Theodore J. Price, et al.serum albumin in PBS, and passed through an LS column (Miltenyi, 130-042-401) on a MidiMACS separator (130-042-301) according to manufacturer's protocol. Samples were then spun down and resuspended in PBS and proceeded

(8) CD103+CD8+ tissue‐resident memory T lymphocytes of melanoma boost anti‐tumour immunity and predict immunotherapy outcomesClinical and Translational MedicineSeptember 23, 2025Tianyi Zhang, Junquan Song, Yinlam Li, Kangjie Shen, Jiangying Xuan, Yuan Gao, Lili Lu, Zhi Pang, Lu Wang, Yang Yang, et al.(Cat#130‐121‐223; Cat#130‐124‐089; Cat#130‐111‐684; Cat#130‐090‐485, Miltenyi) for sorting (Cat#130‐042‐401, Cat#130‐042‐301, Miltenyi). For T lymphocytes requiring reactivation, cells were activated using magnetic microbeads

(9) A Protocol for Modeling Human Bone Inflammation: Co-Culture of Osteoblasts and Osteoclasts Exposed to Different Inflammatory MicroenvironmentsMethods and ProtocolsSeptember 1, 2025Araceli Valverde, Afsar Raza NaqviNo.: 130-042-401)MidiMACS™ Starting Kit (LS) (Miltenyi Biotec, Bergisch Gladbach, Germany Cat. No.: 130-042-301) 2.3. Cell Viability Assay Cell viability assays were performed using the CellTiter 96 AQueous Cell

(10) EBI2-oxysterol signalling regulates VE-cadherin expression and multiple sclerosis CD4+ T cell attachment to a human tri-cell spheroid blood-brain barrier modelBrain, Behavior, & Immunity - HealthAugust 1, 2025Fionä Caratis, Inez Mruk, Klaudia Konieczna-Wolska, Bartłomiej Rojek, Marek Hałas, Paulina Czaplewska, Bartosz Karaszewski, Tomomi Furihata, Aleksandra Rutkowskacells were then applied to a column (130-093-545, Miltenyi Biotec) and placed in a magnetic field (130-042-301). 4 ml of the elution buffer (130-093-545, Miltenyi Biotec) was used to flush out the labelled cells

(11) Mucosal Mixed Lymphocyte Reaction Assay Using Intestinal Lymphocytes as a Biomarker for Intestinal Transplant Tolerance DevelopmentTransplantationJuly 1, 2025M. Esad Gunes, Satyajit Patwardhan, Julie Hong, Elin Manell, Philip Jordache, Ishit Chauhan, Ahmed Almesallmy, Jianing Fu, Megan Sykes, Joshua Weiner130-042-401) and placed in a MidiMACS™ Magnetic Separator (Miltenyi Biotec, Gaithersburg, MD, Cat: 130-042-301) according to manufacturer guidelines. Positively selected CD25+ cells were discarded, and negatively

(12) Lipid‐associated macrophages are more abundant in subcutaneous than visceral adipose tissue in patients with obesityObesity (Silver Spring)June 29, 2025Marjorie Reyes‐Farias, Pablo Fernández‐García, Patricia Corrales, Lorena González, David Navarro‐Sanagustín, Andrea Soria‐Gondek, Silvia Pellitero, Jordi Tarascó, Pau Moreno, José M. Balibrea, et al.cells. Magnetic labeling and separation were performed using a MidiMACS kit with LS column (Miltenyi Biotec, 130‐042‐301) according to the manufacturer's instructions. The eluted fraction enriched with CD11b+ cells was cooled

(13) Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integritySci AdvMay 23, 2025Lata Adnani, Brian Meehan, Minjun Kim, Dongsic Choi, Christopher E. Rudd, Yasser Riazalhosseini, Janusz Rak(catalog no. 130-042-401, Miltenyi Biotec) on the MidiMACS separator attached to a multistand (catalog no. 130-042-301, Miltenyi Biotec). Afterward, 3 ml of buffer was used to wash the column three times before collecting

(14) Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesiclesSTAR ProtocApril 17, 2025Federica Zanotti, Ilaria Zanolla, Massimo Bonora, Claudia Morganti, Barbara Zavan, Letizia Ferroni, Paolo Pinton, Keisuke Itoround-bottom tube with cell-strainer cap,Falcon,Cat# 352235 MidiMACS Starting Kit (LS),Miltenyi Biotec,Cat# 130-042-301 SmartSEC Mini EV Isolation System,System Biosciences,Cat# SSEC100A-1 Formvar/carbon-supported copper

(15) Regulatory T cells inhibit FoxP3 to increase the population of tumor initiating cells in hepatocellular carcinomaJournal of Cancer Research and Clinical OncologyJuly 29, 2024Chang Liu, Yi-jun Tu, Hong-Yang Cai, Yan-yan Pan, Yuan-yuan Wu, Li Zhangpositive selection of CD4+ CD25+ CD127dim/– Treg cells using MidiMACS™ Separator and Starting Kits (130-042-301, Miltenyi Biotec). Tregs were expanded in X-VIVO™ 15 medium (BE02-060 F, Lonza, Basel, Switzerland)

(16) Beneficial islet inflammation in health depends on pericytic TLR/MyD88 signalingJournal of Clinical InvestigationJuly 15, 2024Anat Schonblum, Dunia Ali Naser, Shai Ovadia, Mohammed Egbaria, Shani Puyesky, Alona Epshtein, Tomer Wald, Sophia Mercado-Medrez, Ruth Ashery-Padan, Limor Landsmanthen applied to a MACS column (Miltenyi Biotec, 130-042-401) in a MACS separator (Miltenyi Biotec, 130-042-301). After removal from the separator, the columns were washed, and the magnetically labeled cells were

(17) High-Resolution Fluorespirometry to Assess Dynamic Changes in Mitochondrial Membrane Potential in Human Immune CellsJournal of Visualized ExperimentsMay 24, 2024Ana P. Valencia, Gavin Pharaoh, Arthur F. Brandao, David J. Marcinek,LS Columns,Miltenyi Biotec,130-042-401 Cell isolation,Multi-MACS stand and MidiMACS Separator,Miltenyi Biotec,130-042-301 Mir05 buffer,"Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)",Sigma-Aldrich,E4378 Mir05

(18) Sinomenine Hydrochloride Protects IgA Nephropathy Through Regulating Cell Growth and Apoptosis of T and B LymphocytesDrug design, development and therapyJanuary 1, 2024Li JJ, Li L, Li S, Tang XY, Sun HF, Liu JX.(CW0130S) was purchased from Beijing Kangwei Century Biotechnology Co., Ltd. MidiMACS™ Starting Kits (LS: 130–042-301, LD: 130–090–329), Pan T Cell MicroBeads (130–090-320), and CD45RA MicroBeads (130–090-494) were all

(19) Protocol for acquiring samples to assess the impact of microplastics on immune microenvironments in the mouse intestineSTAR ProtocolsDecember 1, 2023Akihito Harusato, Wooseok Seo, Hirohito Abo, Yoshitaka Nakanishi, Hiroyoshi Nishikawa, Yoshito Itohtubes,N/A,N/A 15 mL tubes,N/A,N/A MACS MultiStand,Miltenyi,#130-042-303 MidiMACS separator,Miltenyi,#130-042-301 LS columns,Miltenyi,#130-042-401 NanoDrop 2000,Thermo Scientific,N/A Orbital shaker,WakenBtech,StackShakeC Centrifuge,TOMY,AX-310 Vortex,Scientific

(20) Reprogramming Cancer Cells to Antigen-presenting CellsBio-protocolNovember 20, 2023Ferreira AG, Zimmermannova O, Kurochkin I, Ascic E, Åkerström F, Pereira CF.(Miltenyi Biotec, catalog number: 130-042-303)MidiMACSTM separator (Miltenyi Biotec, catalog number: 130-042-301)4–16K refrigerated centrifuge (Sigma, catalog number: 10474)Automatic cell counter (Corning, CytoSmart,

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MidiMACS™ Starting Kit (LS)

MidiMACS™ Starting Kit (LS) from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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MidiMACS™ Starting Kit (LS) from Miltenyi Biotec

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