Pre-Separation Filters (30 µm) from Miltenyi Biotec

(1) Purification of murine cortex mitochondria using a modified magnetic bead isolation method☆MethodsXJune 1, 2026Eva Josic, Ana Ujevic, Borna Puljko, Nikolina Maček Hrvat, Adriana Lipovcic, Svjetlana Kalanj-Bognar, Kristina Mlinac-JerkovicCat#130–042–303) LS Columns (Miltenyi Biotec, Cat#130–042–401) Pre-Separation Filters (30 µm) (Miltenyi Biotec, Cat#130–041–407) Hettich Universal 32 R Centrifuge Biorad ChemiDoc™ MP Imaging System Olympus FV3000 confocal microscope Procedure Buffer

(2) Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cellsSTAR ProtocApril 24, 2026Christina Pfab, Jonas M. Holzinger, Sarah Hirsch, Katharina U. Roithmeier, André Gessner, Sigrid Bülow130-042-303 LS Columns,Miltenyi Biotec,Cat# 130-042-401 Pre-Separation Filters (30 μm),Miltenyi Biotec,Cat# 130-041-407 FACS Aria II,BD Biosciences,N/A BD FACSCanto II,BD Biosciences,N/A Luminex 100 system,Luminex corp.,N/A Falcon

(3) Mitochondrial transfer from glia to neurons protects against peripheral neuropathyNatureFebruary 26, 2026Jing Xu, Yize Li, Charles Novak, Min Lee, Zihan Yan, Sangsu Bang, Aidan McGinnis, Sharat Chandra, Vivian Zhang, Wei He, et al.debris were removed by centrifugation. The supernatant was filtered through a pre-separation filter (130-041-407; Miltenyi Biotec) to obtain the crude mitochondrial fraction. For immunocapture of mitochondria against

(4) Human hippocampal neurogenesis in adulthood, ageing and Alzheimer’s diseaseNatureFebruary 25, 2026Ahmed Disouky, Mark A. Sanborn, K. R. Sabitha, Mostafa M. Mostafa, Ivan Alejandro Ayala, David A. Bennett, Yisha Lu, Yi Zhou, C. Dirk Keene, Sandra Weintraub, et al.incubated on ice for 5 min. The homogenate was then filtered through 70 µm filters (Miltenyi Biotec 130-041-407) and 40 µm filters (Miltenyi Biotec 130-041-406), moved to 1.5 ml tubes and centrifuged at 4 °C for

(5) Vitamin D deficiency alters prostate epithelial differentiation and increases prostate cancer aggressiveness in ex vivo and in vivo modelsbioRxivFebruary 20, 2026Adriana Duraki, Kirsten D. Krieger, Sasha Celada, Robert A. Holt, Ryan M. Brown, Luyu Wang, Michael J. Schlicht, Maarten C. Bosland, Robert M. Sargis, Donald Vander Griend, et al.Dispase (STEMCELL 07923). Single cells were isolated through a 30-micron cell filter (Miltenyi Biotec 130–041-407) four times. Cell suspensions were then transferred to DNA LoBind Tubes (Eppendorf 022431021) and centrifuged

(6) Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatosNature NeuroscienceFebruary 17, 2026Jackson W. Mace, Sachin P. Gadani, Matthew D. Smith, Danny Galleguillos, Bong Gu Kang, Milton Roy, Meilian Liu, Benjamin Summers, Thomas Garton, Marjan Gharagozloo, et al.in water), pipette-mixed 20 times then passed over a 30 μM Pre-Separation Filter (Miltenyi, cat. no. 130-041-407). Nuclei were again counted with 5 μg ml−1 acridine orange. Next, all samples were equally pooled based

(7) Impaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imagingNature CommunicationsJanuary 28, 2026Liza Sarde, Gaëlle Letort, Hugo Varet, Vincent Laville, Julien Fernandes, Shahragim Tajbakhsh, Brendan Evano°C. MuSCs were then dissociated mechanically using a P1000 pipette, filtered through a 30 μm filter (130-041-407, Miltenyi) into a 15 mL falcon, centrifuged at 500 g for 15 min at 21 °C and resuspended in 500 μl differentiation

(8) Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEXResearch SquareDecember 23, 2025Matthew R. Skene, Zachary J. Thomson, Saransh N. Thomson, Saransh Ilcisin, Matthieu Landreau, Tyanna J. Landreau, Peter J. Stuckey, Micheal Wittig, Chase D. Keller, Troy R. Torgerson, et al.concentrations, washed in Post-Hyb resuspension buffer, and strained using 30 μM strainers (Miltenyi, 130-041-407) as noted in the 10x User Guide CG000673, Rev B. The single cell suspension pool was loaded following

(9) REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived ImmunitybioRxivDecember 2, 2025Matthew R. Hart, Zachary J. Thomson, Saransh N. Kaul, Saskia Ilcisin, Matthieu Landreau, Tyanna J. Stuckey, Peter J. Wittig, Micheal Keller, Chase D. McCann, Troy R. Torgerson, et al.concentrations, washed in Post-Hyb resuspension buffer, and strained using 30 μM strainers (Miltenyi, 130–041-407) as noted in the 10x User Guide CG000673, Rev B. The single cell suspension pool was loaded following

(10) IPSC-derived organoid-sourced skin cells enable functional 3D skin modeling of recessive dystrophic epidermolysis bullosaJ Tissue EngDecember 1, 2025Laura Garriga-Cerda, Alberto Pappalardo, Charlotte Y. Lee, Jeffrey Kysar, Kristin Myers, Hasan Erbil Abaciback in the incubator. After 30 min, the solution was strained with 30 µm filters (Miltenyi Biotec #130-041-407) and pelleted before starting MACS sorting. Briefly, the pellet was resuspended in 500 µl of MACS buffer

(11) Targeting SERPINB3–MAPK axis-mediated cuproptosis resistance enhances the response to antitumor immunotherapyMolecular CancerNovember 22, 2025Xiao-Xiao Huang, Cheng-Ke Xie, Yi-Chao Mo, Wei Li, Yong-Ding Wu, Zhi-Yuan Li, Hao-Xiang Zhang, Ge Li, Long Jin, Xin-Quan Lin, et al.instructions. The cells were resuspended in buffer and passed through preseparation filters (30 μm, Miltenyi, 130-041−407) to remove cell clumps that might clog the column. Dead cells were removed via a Dead Cell Removal Kit

(12) Circulating tumor cells as predictive biomarkers in the risk stratification of DCIS: Evidence of early disseminationSci AdvOctober 31, 2025Neha Nagpal, Brittany Rupp, Yan Hong, Jamie Wagner, Kelsey Larson, Christa Balanoff, Allison Aripoli, Ashley Huppe, Onalisa Winblad, Jessica Peterson, et al.sufficient cells were isolated. 500 μl of PBS was passed through the preseparation filter (Miltenyi Biotec, 130-041-407) and then the cell suspension was passed through the same filter while it was placed on a 15-ml conical.

(13) Advancing Esophageal Disease Modeling: Microfluidic Platforms for Adult Tissue-Resident Stem Cell Culture and DifferentiationGastro Hep AdvancesOctober 23, 2025Sara Hosseini-Farahabadi, Omar Abuhussein, Corina Stewart, Guang Gao, Tenanye Haglund, David Schaeffer, Fergal Donnellan, Frank McKeon, Wa Xian, Dermot Kelleher, et al.shaking. The resultant cell suspension was filtered through a 30 μm cell strainer (Miltenyi Biotec; cat#130-041-407) under centrifugation prior to resuspension in ASC culture media composed of c-FAD media8, 9, 10 modified

(14) EZH2-TTP-mTORC1 Axis Drives Phenotypic Plasticity and Therapeutic Vulnerability in Lethal Prostate CancerbioRxivAugust 11, 2025Beatriz German, Katherine L. Morel, Teia Noel, Nadia Boufaied, Deborah L. Burkhart, Sujun Chen, Felipe Dezem, Xintao Qui, Henry W. Long, Stefan DiFazio, et al.adding 1 mL of ST-SB. The sample was filtered through a pre-wetted 30 μm filter (Miltenyi Biotec, cat: 130–041-407) into a 15 mL conical tube. The homogenizer was rinsed 3× with 1 mL of ST-SB and this was transferred

(15) The gene regulatory landscape driving mouse gonadal supporting cell differentiationSci AdvJuly 25, 2025Isabelle Stévant, Elisheva Abberbock, Meshi Ridnik, Roni Weiss, Linoy Swisa, Christopher R. Futtner, Danielle M. Maatouk, Robin Lovell-Badge, Valeriya Malysheva, Nitzan Gonengentle pipetting. The cells were filtered through a 30-μm strainer (Miltenyi Biotec, catalog no. no. 130-041-407) and collected into a polypropylene fluorescence-activated cell sorting (FACS) tube (BD Falcon, catalog

(16) Senescent macrophages induce ferroptosis in skeletal muscle and accelerate osteoarthritis-related muscle atrophyNature AgingJune 27, 2025Wei Xiang, Tongyi Zhang, Bingfei Li, Song Li, Bin Zhang, Shunzheng Fang, Lifeng Chen, Yunquan Gong, Bo Huang, Daibo Feng, et al.equal volume of FBS. Then, the solution went through a 30-μm pre-separation filter (Miltenyi Biotec, 130-041-407) without pressure to move the debris and was centrifuged at 500g for 5 minutes. The precipitate in the

(17) Protocol for isolation of endothelial cells from adult mouse brainSTAR ProtocolsJune 20, 2025Audrey G. Miglietta, Jennifer David-Bercholz, Roeben N. Munji, Robert S. Pulido, Allison L. Soung, Niccolò Terrando, Richard Daneman, Ting Yang130-091-051), LS Columns (Miltenyi Biotec, 130-042-401), and 30 μm Pre-Separation Filters (Miltenyi Biotec, 130-041-407) in the configuration shown in Figure 1B.•Performing fluorescence-activated sorting requires a cell

(18) Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transportersSTAR ProtocolsJune 20, 2025Cheng Ma, Ramin Banan Sadeghian, Ryosuke Negoro, Kazuya Fujimoto, Toshikazu Araoka, Naoki Ishiguro, Minoru Takasato, Ryuji YokokawaONE,3-3321-02 MS columns,Miltenyi Biotec,130-042-201 Pre-separation filters (30 μm),Miltenyi Biotec,130-041-407

(19) Cholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafishNat CommunJune 6, 2025Sema Elif Eski, Jiarui Mi, Macarena Pozo-Morales, Gabriel Garnik Hovhannisyan, Camille Perazzolo, Rita Manco, Imane Ez-Zammoury, Dev Barbhaya, Anne Lefort, Frédérick Libert, et al.undissociated chunks and debris, the solution was passed through a 40 μm cell strainer (Miltenyi Biotec, 130-041-407). Cells were pelleted by centrifugation at 500 g for 5 min at 4 °C with soft-stop setting. The supernatant

(20) Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow MonocytesJournal of Visualized ExperimentsMarch 28, 2025Erin B. Taylor, Robert W. Spitz, Katherine R. O'Quinn, Alan J. Moutonreagent" OctoMACS Separator,Miltenyi Biotec,130-042-108, Pre-separation filters (30 μm),Miltenyi Biotec,130-041-407, QuadroMACS Separator,Miltenyi Biotec,130-091-051, Red Blood Cell Lysis Solution (10x),Miltenyi Biotec,130-094-183, Seahorse

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Pre-Separation Filters (30 µm)

Pre-Separation Filters (30 µm) from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Pre-Separation Filters (30 µm) from Miltenyi Biotec

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