SbfI-HF from New England Biolabs

Product Specs
ItemSbfI-HF
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR3642S
Concentration20,000 units/ml
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CCTGCA⇓GG
GG⇑ACGTCC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) RASP: rapid antibody functional screening by pentavalent phage displaymAbsDecember 31, 2026Manpreet Kaur, Abhishek Dubey, Kartik Chandrangene ccdB to get minimal self-ligation,13 and 2) added two new restriction enzyme sites; SbfI (NEB, #R3642S) at 5’ and PspXI (NEB, #R0656S) at 3’end of ccdB (named as pDL23c-ccdB phagemid) (Supplementary Figure

(2) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapatwas achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S); Δ TATA promoter using SbfI-HF (NEB, #R3642S) and BglII. For insertion of RMND5A and ANAPC1 sequences into the pGL3 vector, digestion was performed

(3) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.extension. The final assembled amplicon was digested using BsiWI-HF (NEB, R3553S) and SbfI-HF (NEB, R3642S) and ligated into the pITR2-Rep2-dead(GFP)Cap6 backbone using T4 DNA ligase (NEB, M0202S). The pITR2-Rep2-dead(GFP)Cap6contains

(4) Florigen and antiflorigen gene expression correlates with reproductive state in a marine angiosperm, Zostera marinaiScienceAugust 15, 2025Christine T. Nolan, Ian Campbell, Anna Farrell-Sherman, Bryan A. Briones Ortiz, Chansie Yang, Kerry A. Naish, Verónica S. Di Stilio, James E. Kaldy, Cinde Donoghue, Jennifer L. Ruesink, et al.DNA Library Prep Kit,NEB,Cat# E7645S/L T4 DNA Ligase & Buffer,NEB,Cat# M0202M Sbf1-HF restriction enzyme,NEB,R3642S Gateway™ LR Clonase™ II Enzyme Mix,ThermoFisher,Cat# 11791020 GeneJET Plasmid Mini Prep Kit,ThermoFisher,FERK0503 Phusion

(5) Enhancing the stability of continuous fermentations for platform chemical productioniScienceMarch 21, 2025Victoria Outram, Andrew Yiakoumetti, Charlotte Green, Rebekah King, John M. Ward, Alex Conradie(BamHI-HF variant),New England Biolabs UK Ltd,R3136S SbfI (SbfI-HF variant),New England Biolabs UK Ltd,R3642S BglII,New England Biolabs UK Ltd,R0144S SapI,New England Biolabs UK Ltd,R0569S T4 DNA Ligase,New England Biolabs

(6) Optimizing Yeast Homologous Recombination for Splicing Large Coronavirus Genome FragmentsInternational Journal of Molecular SciencesDecember 23, 2024Guoqing Xiong, Xuan Huang, Ao Hu, Zhixin Meng, Jiazhen Cui, Yuzhong Feng, Zhili Chen, Yuanyuan Lu, Qi Yang, Gang LiuIpswich, USA) was utilized for the extraction of the YAC plasmid. Quantities of 1 μL of Sbf I-HF (NEB, R3642S, Ipswich, USA) each, 5 μL of rCutSmart, and 2 μg of YAC plasmid were combined with 50 μL of nuclease-free

(7) Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in S. cerevisiaebioRxivDecember 22, 2024Rachel Baum, Jinyoung Kim, Ryan Y. Muller, Nicholas T. IngoliarCutSmart, one digested with 1.0 μl EagI-HF (NEB # R3505S) and the other with 1.0 μl SbfI-HF (NEB #R3642S). Digests were incubated for 2.5 hours at 37 °C and DNA was purified using a Monarch PCR & DNA Cleanup

(8) A genome-wide screen links peroxisome regulation with Wnt signaling through RNF146 and TNKS/2Journal of Cell BiologyOctober 7, 2024Jonathan T. Vu, Katherine U. Tavasoli, Connor J. Sheedy, Soham P. Chowdhury, Lori Mandjikian, Julien Bacal, Meghan A. Morrissey, Chris D. Richardson, Brooke M. GardnerMacherey-Nagel) and prepared as previously described (Kampmann et al., 2014) with modifications: Sbf1 (R3642S; NEB) was used instead of PvuII for the restriction digest. Next-generation sequencing (NGS) was performed

(9) The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle traffickingCell ReportsAugust 27, 2024Khavong Pha, Kathleen Mirrashidi, Jessica Sherry, Cuong Joseph Tran, Clara M. Herrera, Eleanor McMahon, Cherilyn A. Elwell, Joanne N. Engel,Vector Laboratories,H-1500–10 Ampicillin,Sigma,A9518 Cycloheximide,Sigma,239765 Spectinomycin,Sigma,S4014 Sbf1-HF,New England Biolabs,R3642S EagI-HF,New England Biolabs,R3505S EcoR1-HF,New England Biolabs,R3101S Not-HF,New England Biolabs,R3189S HindIII-HF,New England Biolabs

(10) Detecting material state changes in the nucleolus by label-free digital holographic microscopyEMBO ReportsApril 23, 2024Christiane Zorbas, Aynur Soenmez, Jean Léger, Christophe De Vleeschouwer, Denis LJ LafontaineLatrunculin A,Sigma-Aldrich,428021 Lipofectamine 3000,Thermo Fisher Scientific,L3000001 InFusion Snap assembly,Takara,638947 SbfI-HF,NEB,R3642S XhoI,NEB,R0146S Lab-Tek chambered coverglass slides (8 well),Thermo Fisher Scientific,155411 Ibidi

(11) Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factorRetrovirologyAugust 22, 2023Joy Twentyman, Anthony Khalifeh, Abby L. Felton, Michael Emerman, Molly Ohainleconstructs were cloned in pQCXIP (TaKaRa Bio, San Jose, CA, #631516) by SbfI (New England BioLabs #R3642S) and NotI (New England Biolabs #R3189S) restriction digest. pQCXIP-TRIM34ΔSPRY constructs were transfected

(12) Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factorbioRxivMay 7, 2023Joy Twentyman, Anthony Khalifeh, Abby L. Felton, Michael Emerman, Molly OhAinleconstructs were cloned in pQCXIP (TaKaRa Bio, San Jose, CA, #631516) by SbfI (New England BioLabs #R3642S) and NotI (New England Biolabs #R3189S) restriction digest. pQCXIP-TRIM34ΔSPRY constructs were transfected

(13) The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosisNature communicationsJuly 16, 2022De Marco Zompit M, Esteban MT, Mooser C, Adam S, Rossi SE, Jeanrenaud A, Leimbacher PA, Fink D, Shorrocks AK, Blackford AN, et al.lacking the codons of the indicated amino acids, into pIRESneo2-EGFP-TOPBP1-WT using SbfI-HF® (NEB, R3642S) and StuI (NEB, R0187S) restriction enzymes. pcDNA3.1(+)-N-HA-TOPBP1(740-899) was custom-synthesized

(14) FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expressionCell reportsFebruary 15, 2022Malikov V, Meade N, Simons LM, Hultquist JF, Naghavi MH.,Thermo Fisher Scientific,cat #78833 EcoRI-HF,New England BioLabs,cat #R3101S SbfI-HF,New England BioLabs,cat # R3642S NotI-HF,New England BioLabs,cat #R3189S "Pierce Protease Inhibitor Mini Tablets, EDTA-free",Thermo Fisher Scientific

(15) Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiomeCellFebruary 1, 2022Wen-Bing Jin, Ting-Ting Li, Da Huo, Sophia Qu, Xin V. Li, Mohammad Arifuzzaman, Svetlana F. Lima, Hui-Qing Shi, Aolin Wang, Gregory G. Putzel, et al.,Zymo Research,Cat# D6005 Quick RNA fungal/bacterial kit,Zymo Research,Cat# R2010 SbfI restriction enzyme,NEB,Cat# R3642S SYBR green chemistry,Applied Biosystems,Cat# 4368706 T4 DNA Ligase (HC),Promega,Cat# M1794 XbaI restriction

(16) Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systemsNature CommunicationsDecember 1, 2021Timothy C. Yu et al.(#M0543S) for 10 cycles using primers GU 132 and GU 134. Library ends were then digested with SbfI-HF (NEB #R3642S) and XhoI (NEB #R0146S) by incubating at 37 °C for 1.5 h. The plasmid vector, pLibAcceptorV2, was first

(17) DNA methylation patterns in juvenile systemic sclerosis and localized sclerodermaClinical ImmunologyJuly 1, 2021Patrick Coit, Kaila L. Schollaert, Emily M. Mirizio, Kathryn S. Torok, Amr H. Sawalhawas used as a backbone for the in vitro patch methylation experiments. Restriction enzymes SbfI-HF (R3642S; New England BioLabs; Ipswich, MA, USA) and SpeI-HF (R3133S; New England BioLabs; Ipswich, MA, USA)

(18) Increased expression of PolÎ´ does not alter the canonical replication program in vivoWellcome Open ResearchMay 4, 2021RÃ³bert Zach, Antony M. CarrApaI (NEB, R0114S); XhoI (NEB, R0146S); SacI-HF (NEB, R3156S); SpeI-HF (NEB, R3133S); SbfI-HF (NEB, R3642S); SalI-HF (NEB, R3138S); BamHI-HF (NEB, R3136S). Ligation products were transformed into DH5-Alpha E. coli

(19) A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoirCell reports. MedicineApril 20, 2021Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, et al.,New England BioLabs,Cat#R0547S SalI-HF,New England BioLabs,Cat#R3138S SbfI-HF,New England BioLabs,Cat#R3642S XmaI,New England BioLabs,Cat#R0180S BssSαI,New England BioLabs,Cat#R0680 XhoI,New England BioLabs,Cat#R0146S PspXI,New England BioLabs

(20) Increased expression of Polδ does not alter the canonical replication program in vivoWellcome Open ResearchFebruary 26, 2021Róbert Zach, Antony M. CarrApaI (NEB, R0114S); XhoI (NEB, R0146S); SacI-HF (NEB, R3156S); SpeI-HF (NEB, R3133S); SbfI-HF (NEB, R3642S); SalI-HF (NEB, R3138S); BamHI-HF (NEB, R3136S). Ligation products were transformed into DH5-Alpha E. coli

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SbfI-HF

SbfI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (29)

Reviews

SbfI-HF from New England Biolabs

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