RsaI from New England Biolabs

Product Specs
ItemRsaI
CompanyNew England Biolabs
Price $273.00 Inquire
Catalog NumberR0167L
Quantity5000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GT/AC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Leptin receptor deficiency induces early, transient and hyperglycaemia-independent blood-brain barrier dysfunctionScientific reportsFebruary 27, 2019Corem N, Anzi S, Gelb S, Ben-Zvi A.CAT AGT TTA GGT TTG TGT-3′. PCR products were restricted with RsaI restriction enzyme (NEB, cat no. R0167). DNA fragments were separated on 3% agarose gel. All mice were normoglycemic at 3 weeks. Only hyperglycemic

(42) Association between antidiabetic agents use and leukocyte telomere shortening rates in patients with type 2 diabetesAging (Albany NY)January 28, 2019Juanhong Liu, Yuanlong Ge, Shu Wu, Delin Ma, Weijie Xu, Ye Zhang, Yan Yang(Axygen, Corning, Inc., NY, USA). Hinf I (R0155L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167L, New England BioLabs) were used to digest the genomic DNA at 37°C overnight. Then, 0.7% agarose gel electrophoresis

(43) Divergent patterns of telomere shortening in tropical compared to temperate stonechatsEcology and EvolutionJanuary 1, 2019Beate Apfelbeck, Mark F. Haussmann, Winnie Boner, Heiner Flinks, Kate Griffiths, Juan Carlos Illera, Kim G. Mortega, Zachary Sisson, Patrick Smiddy, Barbara Helmusing the TRF assay. A 10 µg quantity of DNA was digested using 1.0 ml of RsaI (New England Biolabs, R0167L) and 0.2 ml of HinfI (New England Biolabs, R0155M) in CutSmart Buffer (New England Biolabs, B7204S)

(44) HFE GENE MUTATIONSThe Professional Medical JournalJanuary 12, 2018Nadir Ali, Bashir Ahmed, Humaira Akram, Junaid Akhtar, Ross Williams, Ron DixonPCR product of 294 bp (H63D) and 355 bp (C282Y) was digested using 4U of MboI (NEB, R0147S) or 2U RsaI (NEB, R0167S), respectively, in 2.5 μl NEBuffer 4 in a final volume of 25 μl, and incubating at 37°C 6 hours or overnight.

(45) Prenatal stress and newborn telomere lengthAmerican Journal of Obstetrics and GynecologyJuly 1, 2016Nicole M. Marchetto, Rebecca A. Glynn, Mackenzie L. Ferry, Maja Ostojic, Sandra M. Wolff, Ruofan Yao, Mark F. Haussmannrestriction fragment assay (34). Ten μg of DNA was digested using 1.0 μl of RsaI (New England Biolabs, R0167L) and 0.2 μL of HinfI (New England Biolabs, R0155M) in CutSmart Buffer (New England Biolabs, B7204S)

(46) Telomere Restriction Fragment (TRF) AnalysisBio-protocolNovember 20, 2015Mender I, Shay JW.(New England BioLabs, catalog number: R0108L) e. RsaI 10,000 units/ml (New England BioLabs, catalog number: R0167L) f. AluI 10,000 units/ml (New England BioLabs, catalog number: R0137L) g. NE Buffer2 10x concentrate

(47) The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assayJournal of immunological methodsOctober 1, 2015Hilton HG, Moesta AK, Guethlein LA, Blokhuis J, Parham P, Norman PJ.large size of the pAcGP67a vector (9761bp). Following the PCR, add 1μl DpnI (New England BioLabs, Cat. #R0167S) to the reaction mixture and incubate for 30min at 37°C. DpnI digests the methylated template DNA and

(48) Flexible and scalable genotyping-by-sequencing strategies for population studiesBMC GenomicsDecember 1, 2014Christopher Heffelfinger, Christopher A Fragoso, Maria A Moreno, John D Overton, John P Mottinger, Hongyu Zhao, Joe Tohme, Stephen L Dellaportahours. Genomic DNA from B73 and the Nipponbarre was digested with MlyI (R0610), AluI (R0137), RsaI (R0167), EcoRV (R0195), StuI (R0187), HaeIII (R0108), and HincII (R0103, New England Biolabs). For the F2 mapping

(49) Single-Cell Semiconductor SequencingMethods in Molecular BiologyMay 1, 2014Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, Leonid L. Moroz(Clontech Cat # 634926). • Alu I and NEB buffer 4 (Cat # R0137S, New England BioLabs). • Rsa I (Cat # R0167S, New England BioLabs). • Ligate-IT Rapid Ligation Kit (Cat # 78400, Affymetrix). • 5× Quick Ligation

(50) Oncogenic HPV Types Infection in Adolescents and University Women from North Portugal: From Self-Sampling to Cancer PreventionJournal of OncologyNovember 28, 2011Jani Silva, Joana Ribeiro, Hugo Sousa, Fátima Cerqueira, Ana Luisa Teixeira, Ines Baldaque, Teresa Osório, Rui Medeiros(Fermentas Inc., #ER0151, Canada), DdeI (New England BioLabs, R0175L), and RsaI (New England BioLabs, R0167S). Digestion was performed at 37◦ C for 5 hours. The restricted fragments were separated by electrophoresis

(51) Spatial distribution of ammonia-oxidizing bacteria and archaea across a 44-hectare farm related to ecosystem functioningThe ISME JournalJuly 1, 2011Ella WessÃ©n, Mats SÃ¶derstrÃ¶m, Maria Stenberg, David Bru, Maria Hellman, Allana Welsh, Frida Thomsen, Leif Klemedtson, Laurent Philippot, Sara Hallin

(52) Mycoplasma Bovis Outbreak in a Herd of North American Bison (Bison Bison)Journal of Veterinary Diagnostic InvestigationSeptember 3, 2010Kyathanahalli S. Janardhan, Mike Hays, Neil Dyer, Richard D. Oberst, Brad M. DeBeyNew England Biolabs Inc., Ipswich, MA. g. Catalog no. R0129S, New England Biolabs Inc., Ipswich, MA. h. Catalog no. R0167S, New England Biolabs Inc., Ipswich, MA. i. Catalog no. 1161-1127, Bio-Rad Laboratories, Hercules, CA. j.

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RsaI

RsaI from New England Biolabs

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RsaI from New England Biolabs

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