RsaI from New England Biolabs

Product Specs
ItemRsaI
CompanyNew England Biolabs
Price $273.00 Inquire
Catalog NumberR0167L
Quantity5000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GT/AC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeneyno. R0108T), NdeI (NEB, catalog no. R0111S), SwaI (NEB, catalog no. R0604S), RsaI (NEB, catalog no. R0167S). Restriction enzymes digestions were prepared by combining 10 × NEB reaction buffer, 500 ng of MiCath

(22) Genomic analyses reveal three phylogenetic species and their evolutionary histories in the big-headed turtleiScienceAugust 18, 2023Shiping Gong, Chatmongkon Suwannapoom, Minh Le, Truong Quang Nguyen, Yan Ge, Yufeng Wei, Yangchun GaoWHP-1.17113.023 Premix Taq™,TaKaRa,Cat# RR901A Agarose G-10,Biowest,Cat# BY-R0100 RsaI,NEB,Cat# R0167L ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, DNeasy Blood

(23) Break-induced replication orchestrates resection-dependent template switchingNatureJuly 1, 2023Zhang T, Rawal Y, Jiang H, Kwon Y, Sung P, Greenberg RA.genomic DNA was digested with Hinf I (New England Biolabs, R0155S) and Rsa I (New England Biolabs, R0167L). For constant-field gel electrophoresis, the digested DNA was separated using the Owl™ A5 Large Gel

(24) Nanopore-Based Fingerprint Immunoassay Based on Rolling Circle Amplification and DNA FragmentationACS NanoMarch 28, 2023Xinqi Kang, Connie Wu, Mohammad Amin Alibakhshi, Xingyan Liu, Luning Yu, David R. Walt, Meni WanunuBeads Phi29 polymerase was purchased from Enzymatics (P7020-HC-L); restriction enzymes AluI (R0137S), RsaI (R0167S), ExoI (M0293S), ExoIII (M0206S), and dNTPs (N0447L) were purchased from New England Biolabs (NEB);

(25) Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysisScientific ReportsDecember 1, 2022Christian Warmt, Ceren Yaslanmaz, Jörg Henkelby using 20 µl purified LAMP product (approx. 2 µg DNA), 2 µl enzyme (10 U/µl; New England Biolabs; R0167S) and 5 µl associated buffer (New England Biolabs; B6004S). Reaction mixture was filled up with ddH2O

(26) Arsenic Pollution and Anaerobic Arsenic Metabolizing Bacteria in Lake Van, the World’s Largest Soda LakeLife (Basel)November 16, 2022Esra Ersoy Omeroglu, Mert Sudagidan, Erdal OgunBiolabs, New England Biolabs, Ipswich, MA, USA, R3101S), RsaI (CutSmart Biolabs, New England Biolabs, R0167S), and HaeIII (CutSmart Biolabs, New England Biolabs, R01108S) restriction enzymes according to manufacturer’s

(27) METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stabilityNucleic acids researchNovember 11, 2022Chen L, Zhang C, Ma W, Huang J, Zhao Y, Liu H.METTL3-deficient or control U2OS cells using phenol–chloroform. A 3 μg aliquot of DNAs was digested with RsaI (R0167L, New England BioLabs), HinfI (R0155L, New England BioLabs) and RNase A, and separated on a 0.7% agarose

(28) A missense variant in the nuclear localization signal of DKC1 causes Hoyeraal-Hreidarsson syndromeNPJ genomic medicineOctober 30, 2022Chu CM, Yu HH, Kao TL, Chen YH, Lu HH, Wu ET, Yang YL, Lin CH, Lin SY, Tsai MM, et al.digested with Hinf I (New England Biolabs, Cat. No: #R0155S) and Rsa I (New England Biolabs, Cat. No: #R0167L) restriction enzyme in 10X CutSmart® Buffer (NEB, Cat. No: #B7204S) at 37 °C overnight. The digested

(29) The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1Nature CommunicationsDecember 1, 2021Sabrina Dietz et al.in 80 µl total volume with 40 U HinfI (New England BioLabs, #R0155) and RsaI (New England BioLabs, #R0167), respectively. The digestion was incubated at 37 °C overnight and the next day additional 10 U of each

(30) Association of leukocyte telomere length with metabolic syndrome in type 2 diabetes mellitusJournal of Research in Medical SciencesJuly 31, 2021Xuemin Dong, Jiaojiao Huang, Jiaojiao Huang, Sanshan Yang, Yan YangUSA). Genomic DNA was digested by Hinf I (R0155 L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167 L, New England BioLabs). Agarose gel electrophoresis was used to isolate the digested DNA. Then, the

(31) Establishment and Maintenance of Gnotobiotic American Cockroaches (Periplaneta americana)Journal of Visualized ExperimentsJune 14, 2021Helen E. Dukes, Josey E. Dyer, Elizabeth A. Ottesenstock solution (32%),Sigma-Aldrich,269336, Petroleum jelly,Vaseline,n/a, RsaI,New England BioLabs,R0167,Includes CutSmart (digestion) buffer Secondary container,n/a,n/a,a plastic container with a lid (such

(32) Telomere erosion in human pluripotent stem cells leads to ATR-mediated mitotic catastropheThe Journal of cell biologyJune 7, 2021Vessoni AT, Zhang T, Quinet A, Jeong HC, Munroe M, Wood M, Tedone E, Vindigni A, Shay JW, Greenberg RA, et al.(10 mM Tris and 1 mM EDTA, pH 8.0). 10 µg extracted DNA was digested overnight with 30 U each RsaI (#R0167; New England Biolabs) and HinfI (#R0155; New England Biolabs) restriction enzymes and resolved on a

(33) Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shorteningNature communicationsMarch 12, 2021Baxley RM, Leung W, Schmit MM, Matson JP, Yin L, Oram MK, Wang L, Taylor J, Hedberg J, Rogers CB, et al.were embedded per plug and digested as described above. Plugs were next digested with either RsaI (NEB R0167) or HinfI (NEB R0155) restriction enzyme in 1x NEB CutSmart Buffer to degrade non-telomeric DNA. Samples

(34) Novel and Efficient Protocol for DNA Coating- Based Identification of DNA-Protein Interaction by Antibody-Mediated ImmunodetectionReports of Biochemistry and Molecular BiologyMarch 2, 2021Niranjan Chellathurai Vasantha, Johnson Retnaraj Samuel Selvan, Saravanakumar Venkatachalam, Jackson Durairaj Selvan Christyraj, Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India.KH2PO4. DNA Coating solution (Thermo Scientific - Cat. No. 17250). Restriction enzymes (Rsa I [NEB - Cat. No. R0167S] and Hinf I [NEB - Cat. No. R0155S]). Primary Antibodies [anti-lamin A antibody (Sigma- Cat. No. L1293)

(35) Single cell imaging of human cancer xenografts using adult immune-deficient zebrafishNature ProtocolsSeptember 1, 2020Chuan Yan, Daniel Do, Qiqi Yang, Dalton C. Brunson, John F. Rawls, David M. LangenauNuclease-free H2O (Life Technologies, cat. no. AM9939) HinfI (NEB, cat. no. R0155S) RsaI (NEB, cat. no. R0167S) Cutsmart buffer (NEB, cat. no. B7204S) Ultrapure Agarose (Life Technologies, cat. no. 165005001)

(36) Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering ToolkitACS Synthetic BiologyMay 15, 2020Binhui Zhao, Pankaj Chaturvedi, David L. Zimmerman, Andrew S. BelmontDpnI, HaeIII, MseI, MspI, RsaI (New England Biolabs, Cat. # R0137S, R0176S, R0108S, R0525S, R0106S, R0167S, respectively) and CutSmart Buffer (New England Biolabs); Terminal Deoxynucleotidyl Transferase and

(37) Shorter leucocyte telomere length as a potential biomarker for nonalcoholic fatty liver disease-related advanced fibrosis in T2DM patientsAnnals of Translational MedicineMay 2, 2020Kun Dong, Ye Zhang, Jiao-Jiao Huang, San-Shan Xia, Yan YangNanodrop. Genomic DNA were digested by Hinf I (R0155L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167L, New England BioLabs) at 37 °C overnight. Then, agarose gel electrophoresis was applied to separate

(38) FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loopsScientific reportsDecember 13, 2019Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, Huang J, Mohan N, Klosek S, Parsa K, et al.Blood Mini Kit (Qiagen, 51106). 2 μg genomic DNA were digested with Hinf I (NEB, R0155) and Rsa I (NEB, R0167) at 37 °C overnight and then run on a 0.8% agarose gel in 1X TAE. After DNA was transferred to an Amersham

(39) The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a MitochondrionMolecular Biology and EvolutionOctober 1, 2019Anna Karnkowska et al.in total volume 200 µl containing five units of the HinfI (NEB, R0155S) and five units of RsaI (NEB, R0167S) restriction enzymes. DNA was purified by ethanol precipitation and resuspended in 15 µl of nuclease-free

(40) Nej1 Interacts with Mre11 to Regulate Tethering and Dna2 Binding at DNA Double-Strand BreaksCell reportsAugust 6, 2019Mojumdar A, Sorenson K, Hohl M, Toulouze M, Lees-Miller SP, Dubrana K, Petrini JHJ, Cobb JA.K,Invitrogen,25530031 Raffinose,US Biological,R1030 RNase A,Sigma,R6513 RsaI,New England Biolabs,R0167S SDS,Avantor,409502 Sodium Acetate,VWR,BDH9278 Sodium Chloride,Fisher Chemical,S64212 Tris base,Fisher

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RsaI

RsaI from New England Biolabs

Sequence

New England Biolabs

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RsaI from New England Biolabs

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