RPMI 1640 Medium, powder from Thermo Fisher Scientific

Product Specs
ItemRPMI 1640 Medium, powder
CompanyThermo Fisher Scientific
Price $28.65Get Pricing Get Quote for this Product
Catalog Number31800089
Quantity10L
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals
TypeMedium

Specifications/Features

Contains:
Contains L-glutamine, but no sodium bicarbonate

Makes:
1 x 10L

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Epigenetic silencing L3MBTL4 sensitizes esophageal cancer to DNA-PKcs inhibitorCancer Biol TherDecember 31, 2026Yuan Li, Aiai Gao, Yuanxin Yao, Meiying Zhang, Lianlian Jin, Qian Tao, Mingzhou Guodetection kit (CYTOCH, #F250003BD, China). ESCC cell lines were cultured in RPMI 1640 medium (Gibco, #31800089, USA) supplemented with 10% fetal bovine serum (Gemini, #900-108, USA) and 1% penicillin/streptomycin

(2) L3MBTL4 methylation is a sensitive marker of DNA-PK inhibitor in pancreatic cancerExplor Target Antitumor TherJuly 23, 2026Yuanxin Yao, Yuan Li, Aiai Gao, Cheng Zhu, Ruijie Wang, Yazhuo Li, Xiaomo Su, Meiying Zhang, Mingzhou GuoLipofectamine 3000 Reagent (Invitrogen, USA). Lentiviral supernatant was then added to the RPMI-1640 medium (31800089, Gibco, USA) containing 10% fetal bovine serum (900-108, GEMINI, USA) and 1% penicillin/streptomycin

(3) Z-DNA binding protein 1 mediates necroptotic cell death in primary murine microglia following herpes simplex virus-1 infectionJ NeurovirolMarch 2, 2026Alexander J. Suptela, Ian Marriottthrough a wire screen in the presence of 0.25% trypsin prior to washing with RPMI 1640 (Gibco, Cat# 31800089) containing 10% fetal bovine serum (FBS; Gibco, Cat# A5256701) and subsequent resuspension in fresh

(4) Peritoneal exosomes mediate activation of CD4+ T cells and enhancement of vaccine efficacy in teleostCell Communication and SignalingFebruary 21, 2026Xueyan Shi, Lixia Jiang, Miao Ye, Arat Yanglang, Marie Kristin Mikkelborg, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan, Roy Ambli Dalmo, et al.a 100 μm sterile nylon gauze filter atop a 50 mL centrifuge tube, moistened with RPMI-1640 medium (31800089, Gibco), and gently pressed and triturated with a pestle to obtain a single-cell suspension. The suspension

(5) Multimodal single-cell protein and RNA profiling unveils dysregulated immature neutrophil dynamics in gestational diabetes mellitusCommunications BiologyJanuary 7, 2026Junyu Xu, Caixia Zhu, Lepei Xie, Yiping Liu, Jinheng Li, Lu Wang, Lisha Ye, Yannan Zhang, Zilian Wang, Limin Zheng, et al.collagenase type IV (C5138; Sigma-Aldrich) and 0.01 mg/mL DNase I (D5025; Sigma-Aldrich) in RPMI medium 1640 (31800089; Thermo Fisher Scientific) for 40 min at 37 °C. The suspensions were strained through nylon mesh and

(6) Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosisFrontiers in ImmunologyJanuary 6, 2026Jin-Sil Park, Hye Yeon Kang, JeongWon Choi, Wonjong Lee, Hyunbin Seong, Sang Hee Cho, Su Beom Lee, Nam Soo Han, Jaekwon Choi, Bo-In Lee, et al.strainer (#93040, SPL, Korea) and maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (#31800089, Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (#16000044, Thermo Fisher Scientific).

(7) Repurposing Antimalarials for Oral Cancer: Selective Efficacy of Hydroxychloroquine on Gingival Squamous Cell CarcinomaInternational journal of molecular sciencesNovember 13, 2025Baroudi S, González Poleo DA, Issa H, Almutairi MH, Semlali A.cells (GC) were routinely grown in RPMI-1640 medium (Gibco, Saint-Laurent, Quebec, QC, Canada, Cat# 31800089), while SCC-9(TC), CAL-27(TC), and GMSM-K cells were maintained in Dulbecco’s modified Eagle’s medium

(8) Nanoliposome loaded with 5-fluorouracil and Dorema aucheri extract reduces tumor growth in a mouse model of colorectal cancerSci RepOctober 7, 2025Fatemeh Keshavarz, Firouzeh Nasimi, Hassan Bardania, Mohsen Soltanshahi, Kamran Goudarzi, Erfan Azadifar, Niloufar Mohamadi, Parisa Ramiyan, Ghasem GhalamfarsaDPPC (850355P) were obtained from Sigma-Aldrich (Germany). Pen/Strep (15070063), RPMI 1640 medium (31800089), FBS (26140079) and PBS (18912014) were obtained from Gibco (USA). Chloroform (1024441000) And 5-fluorouracil

(9) Development of a Modular Ribonucleoprotein Complex as a General Strategy to Deliver RNAi TherapeuticsAdvanced Healthcare MaterialsSeptember 1, 2025Nok Yin Tam, Xiaoqi Wang, Grace Chung Yan Chan, Wai Po Kong, Wai Yin Chau, Xiuqiong Fu, Kwok‐Yin Wong, Hong Lok Lung, Zhi‐Ling Yu, Wei Shen AikMedium (DMEM) medium (12100061, Gibco), and the Panc 02.03 cells were cultured in RPMI 1640 medium (31800089, Gibco). All the media were supplemented with 10% fetal bovine serum (FBS, A5256701, Gibco) and 1x Antibiotic‐Antimycotic

(10) Lipid nanoparticle-delivered intrabodies for inhibiting necroptosis and pyroptosisBiochemical JournalAugust 21, 2025Veerasikku Gopal Deepagan, Xiuquan Ma, Farzaneh Bazregari, Jiyi Pang, Jan Schaefer, Joanne M. Hildebrand, Ruby K. Dempsey, Marcel Doerflinger, Christopher A. Baldwin, Florian I. Schmidt, et al.humidified incubator with 10% CO₂. Colo205 and SW620 cells were maintained in RPMI-1640 (Gibco, Cat# 31800089) supplemented with the 10% (v/v) FBS under identical conditions. While the cell lines were not formally

(11) Lipid nanoparticle-delivered intrabodies for inhibiting necroptosis and pyroptosisBiochem JAugust 20, 2025Veerasikku Gopal Deepagan, Xiuquan Ma, Farzaneh Bazregari, Jiyi Pang, Jan Schaefer, Joanne M. Hildebrand, Ruby K. Dempsey, Marcel Doerflinger, Christopher A. Baldwin, Florian I. Schmidt, et al.humidified incubator with 10% CO₂. Colo205 and SW620 cells were maintained in RPMI-1640 (Gibco, Cat# 31800089) supplemented with the 10% (v/v) FBS under identical conditions. While the cell lines were not formally

(12) Monoclonal Antibodies to Thyrotropin Receptor With Thyroid-Stimulating Activity Activate the NF-κB Pathway to Induce Chemokine ExpressionJ Cell Mol MedJune 11, 2025Yang Yang, Chen HuiChina). SP2/0 mouse myeloma cells and the Nthy‐ori 3‐1 cell line were cultured in RPMI‐1640 medium (31800089, Thermo Fisher Scientific) plus 10% fetal bovine serum (FBS) (10099141C, Thermo Fisher Scientific) and

(13) A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosomeNatureApril 24, 2025Manoj Jangra, Dmitrii Y. Travin, Elena V. Aleksandrova, Manpreet Kaur, Lena Darwish, Kalinka Koteva, Dorota Klepacki, Wenliang Wang, Maya Tiffany, Akosiererem Sokaribo, et al.medium with 0.4% glucose (M2106 Teknova), and RPMI-1640 medium (ThermoFisher Scientific; Catalog number 31800089) supplemented with sodium bicarbonate (2 g/l) and pH adjusted to 7.3-7.4 using 1N HCl. Serum effects

(14) DAP12-associated synthetic antigen receptors enable multi-targeting of T cells with independent chimeric receptors in a small genetic payloadiScienceApril 18, 2025Allyson E. Moore, Hayley Nault, Derek Cummings, Bonnie Bojovic, Nick Serniuck, Christopher L. Baker, Craig Aarts, Chitra Venugopal, Sheila K. Singh, Joanne A. Hammill, et al.Set,eBioscience,Cat# 00-5523-00 Alt-R™ S.p. HiFi Cas9 Nuclease V3,IDT,Cat# 1081061 RPMI 1640 Medium,Gibco,Cat# 31800089 Heat-inactivated fetal bovine serum,Gibco,Cat # 16140071 L-Glutamine,BioShop,Cat# GLU102.1 HEPES,Sigma-Aldrich,Cat#

(15) Discovery of the antifungal compound ilicicolin K through genetic activation of the ilicicolin biosynthetic pathway in Trichoderma reeseiBiotechnol Biofuels BioprodMarch 11, 2025Isabella Burger, Matthias Schmal, Kathrin Peikert, Lukas Fourtis, Christoph Suster, Christian Stanetty, Dominik Schnalzer, Barbara Hufnagel, Thomas Böttcher, Ruth Birner-Gruenberger, et al.Tween-20 (RPMI 1640 with L-glutamine without sodium bicarbonate [Thermo Scientific, Catalog number: 31800089], 20.8 g L−1; MOPS, 69.06 g L−1; glucose, 36 g L−1; Tween-20, 0.004% [v/v]; pH adjusted to 7.0 with

(16) Epigenetic silencing of KCTD8 promotes hepatocellular carcinoma growth by activating PI3K/AKT signalingEpigenomicsJuly 18, 2024Jing Zhou, Meiying Zhang, Aiai Gao, James G Herman, Mingzhou GuoSNU449 and Huh7 cell lines were utilized. All cell lines were cultured in RPMI-1640 medium (Gibco, #31800089) or DMEM (Gibco, #12100061) supplemented with 1% penicillin/streptomycin solution (Biosharp, #BL505A)

(17) A novel inhibitory BAK antibody enables assessment of non-activated BAK in cancer cellsCell Death & DifferentiationJune 1, 2024Hema Preethi Subas Satish, Sweta Iyer, Melissa X. Shi, Agnes W. Wong, Karla C. Fischer, Ahmad Z. Wardak, Daisy Lio, Jason M. Brouwer, Rachel T. Uren, Peter E. Czabotar, et al.Collection;VI USA). RS4;11, MOLM-13, KG-1 and HL-60 cell lines were cultured in RPMI-1640 medium (Gibco #31800089) supplemented with 10% heat-inactivated FCS, 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco #15140122).

(18) Epigenetic silencing schlafen-11 sensitizes esophageal cancer to ATM inhibitorWorld journal of gastrointestinal oncologyMay 15, 2024Zhou J, Zhang MY, Gao AA, Zhu C, He T, Herman JG, Guo MZ.and colo680n. These cells were derived from primary ESCC and cultured in RPMI-1640 medium (Gibco, No. 31800089) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. HEK293 cell line was used

(19) Eugenol as a potential adjuvant therapy for gingival squamous cell carcinomaScientific ReportsMay 13, 2024Hawraa Issa, Lionel Loubaki, Abdullah Al Amri, Kazem Zibara, Mikhlid H. Almutairi, Mahmoud Rouabhia, Abdelhabib SemlaliJapanese male patient with gingival squamous cell carcinoma, was cultured in RPMI 1640 medium (Gibco, 31800089) supplemented with 5% Fetal Bovine Serum (FBS) (Gibco, 12483-020), 0.2% Penicillin/Streptomycin (P/S)

(20) Exploring the anticancer potential of hydrogen sulfide and BAY‑876 on clear cell renal cell carcinoma cells: Uncovering novel mutations in VHL and KDR genes among ccRCC patientsMolecular and Clinical OncologyJanuary 18, 2024Peshraw Salih Hamadamin, Kalthum Asaf Mauloodno. CRL-1932). The cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc.; cat. no. 31800089) which was supplemented with 10% fetal bovine serum (FBS) and 1% of antibiotic (consisting of 10,000-unit

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RPMI 1640 Medium, powder from Thermo Fisher Scientific

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Specifications/Features

Thermo Fisher Scientific

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RPMI 1640 Medium, powder from Thermo Fisher Scientific

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