RPMI 1640 Medium, no folic acid from Thermo Fisher Scientific

Product Specs
ItemRPMI 1640 Medium, no folic acid
CompanyThermo Fisher Scientific
Price $46.60Get Pricing Get Quote for this Product
Catalog Number27016021
Quantity500 mL
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals
TypeMedium

Specifications/Features

Contains:
Contains L-glutamine, but no folic acid.

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi Chenrespectively inoculated into complete medium. (DMEM (Catalog No.: 11965092, Gibco)/RPMI1640 (Catalog No.: 27016021, Gibco) + 10% Fetal Bovine Serum (FBS, Catalog No.:10099158, Gibco) + 1% antibiotics (Catalog No.: 15140148,

(2) Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometryJournal of Biomedical OpticsJanuary 27, 2026Joshua S. Pace, Grace Matheson, Gauri Malankar, Lei Wang, Melissa H. Wong, Summer L. Gibbs, Mark NiedreSigma-Aldrich, St. Louis, MO). All cell lines were cultured in RPMI 1640 folic acid-deficient media (Gibco 27016021; ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gibco 16000044; ThermoFisher

(3) Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometryJ Biomed OptJanuary 27, 2026Joshua S. Pace, Grace Matheson, Gauri Malankar, Lei Wang, Melissa H. Wong, Summer L. Gibbs, Mark NiedreSigma-Aldrich, St. Louis, MO). All cell lines were cultured in RPMI 1640 folic acid-deficient media (Gibco 27016021; ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gibco 16000044; ThermoFisher

(4) Considerations for the use of contrast agents with diffuse in vivo flow cytometry to detect circulating cancer cell populationsbioRxivJuly 31, 2025Joshua S. Pace, Grace Matheson, Gauri Malankar, Lei Wang, Melissa H. Wong, Summer L. Gibbs, Mark NiedreSigma-Aldrich, St. Louis, MO). All cell lines were cultured in RPMI 1640 folic acid deficient media (Gibco 27016021; ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gibco 16000044; ThermoFisher

(5) pH-responsive bond as a linker for the release of chemical drugs from RNA–drug complexes in endosome or lysosomeRNA NanoMedMarch 14, 2025Markey Cancer Center and Department of Surgery, University of Kentucky, Lexington, KY 40536, USA, Piotr Rychahou, Yekaterina Y. Zaytseva, Heidi L. Weiss, B. Mark Evers, Markey Cancer Center and Department of Surgery, University of Kentucky, Lexington, KY 40536, USA;, Sijin Guo, Center for RNA Nanobiotechnology and Nanomedicine, The Ohio State University, Columbus, OH, USA, Yinan Zhang, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China, et al.#CLS3474–24EA); this method stimulated the formation of spheroids within 24 h. RPMI 1640 FA-free medium (#27016021) and dialyzed fetal bovine serum (26400044) were purchased from ThermoFisher Scientific (Waltham, MA).

(6) Use of a universal targeting CAR T cell to simultaneously kill cancer cells and cancer-associated fibroblastsFrontiers in ImmunologyFebruary 17, 2025Bo Huang, Suilan Zheng, Kasireddy Sudarshan, Ramesh Mukkamala, Madduri Srinivasarao, Tushar Sardesai, Xiaofei Yang, Haiyan Chu, Philip S. Low11995073), while MDA-MB-231 and KB cells were cultured in folate-free RPMI 1640 medium (Gibco, Cat# 27016021), all containing 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in 5% CO2 at

(7) In Vivo Labeling and Detection of Circulating Tumor Cells in Mice Using OTL38Molecular Imaging and BiologyAugust 1, 2024Joshua Pace, Jane J. Lee, Madduri Srinivasarao, Shivakrishna Kallepu, Philip S. Low, Mark Niedre(CRL-2974, ATCC, Manassas, VA). All cell lines were cultured in RPMI 1640 folic acid deficient media (Gibco 27016021; ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gibco 16000044; ThermoFisher

(8) Transcobalamin 2 orchestrates monocyte proliferation and TLR4-driven inflammation in systemic lupus erythematosus via folate one-carbon metabolismFrontiers in ImmunologyMay 31, 2024Baoyi Liu, Ang Li, Yi Liu, Xinzhu Zhou, Jingkai Xu, Xianbo Zuo, Ke Xue, Yong Cuidays in either normal RPMI-1640 medium (supplemented with 1 μg/ml FA) or FA-free RPMI-1640 medium (27016021, Thermo Fisher Scientific, USA) or by adding FA (with final concentration of 10 μg/ml FA) to normal

(9) Methotrexate-based PROTACs as DHFR-specific chemical probesCell Chemical BiologyFebruary 15, 2024Sandeep Rana, Patricia Dranchak, Jayme L. Dahlin, Laurence Lamy, Wenqing Li, Erin Oliphant, Jonathan H. Shrimp, Girish H. Rajacharya, Ravi Tharakan, David O. Holland, et al.mL−1 penicillin, 100 μg mL−1 streptomycin, and 1 mM HEPES, or (2) folate acid-free RPMI (Gibco, cat # 27016021) supplemented with 10% dialyzed FBS (v/v), 100 U mL−1 penicillin, 100 μg mL−1 streptomycin, and 1 mM

(10) Novel mutant KRAS addiction signature predicts response to the combination of ERBB and MEK inhibitors in lung and pancreatic cancersiScienceMarch 17, 2023Katarzyna M. Tyc, Aslamuzzaman Kazi, Alok Ranjan, Rui Wang, Said M. SebtiScientific, USA",catalog no. 11966025 RPMI 1640,"Gibco, ThermoFisher Scientific, USA",catalog no. 27016021 ,, Deposited data,Deposited data,Deposited data ,, CCLE mutationdata,Tsherniak et al.56,https://depmap.org/portal/download/(release

(11) Impact of Plastic-Related Compounds on P-Glycoprotein and Breast Cancer Resistance Protein In VitroMoleculesMarch 17, 2023Matteo Rosellini, Petri Turunen, Thomas Efferth(Department of Pediatrics, University of Jena, Jena, Germany) and grown in RPMI 1640 medium (RPMI, 27016021, Gibco™). The multidrug resistance phenotype of CEM/ADR5000 cells has been described [45,46,47]. The

(12) Aptamer-programmable adeno-associated viral vectors as a novel platform for cell-specific gene transferMolecular therapy. Nucleic acidsMarch 14, 2023Puzzo F, Zhang C, Powell Gray B, Zhang F, Sullenger BA, Kay MA.instructions. Cell lines The cells used for the folic acid experiments were grown in RPMI 1640 medium (Fisher, #27016021) supplemented with 10% fetal bovine serum (FBS) and 100 IU/mL penicillin/streptomycin. The rest of the

(13) Development of Adenovirus Containing Liposomes Produced by Extrusion vs. Homogenization: A Comparison for Scale-Up PurposesBioengineering (Basel)October 27, 2022Jaimin R. Shah, Tao Dong, Abraham T. Phung, Tony Reid, Christopher Larson, Ana B. Sanchez, Bryan Oronsky, Sarah L. Blair, Omonigho Aisagbonhi, William C. Trogler, et al.Institute (RPMI) 1640 (Gibco Catalog # 11875093) and RPMI 1640 medium no folic acid (Gibco Catalog # 27016021) were supplemented with 10% FBS and 1% of PSG to prepare the complete RPMI (RP-10) for 4T1 and CT26

(14) Near-infrared diffuse in vivo flow cytometryJournal of biomedical opticsSeptember 1, 2022Pace J, Ivich F, Marple E, Niedre M.human ovarian cancer cell lines. All cells were cultured in RPMI 1640 folic acid deficient media (Gibco 27016021; ThermoFisher Scientific, Waltham, Massachusetts) supplemented with 10% fetal bovine serum (Gibco 16000044;

(15) Multi-Omics Analysis of the Therapeutic Value of MAL2 Based on Data Mining in Human CancersFrontiers in Cell and Developmental BiologyJanuary 17, 2022Jing Yuan, Xiaoyan Jiang, Hua Lan, Xiaoyu Zhang, Tianyi Ding, Fan Yang, Da Zeng, Jiahui Yong, Beibei Niu, Songshu XiaoOVCAR3, and IOSE80 cells were cultured in RPMI 1640 medium (Invitrogen, New York, United States, Cat No. 27016021) supplemented with 10% certified heat-inactivated fetal bovine serum (FBS; Gibco, New York, United States,

(16) Oleic acid restores suppressive defects in tissue-resident FOXP3 Tregs from patients with multiple sclerosisThe Journal of clinical investigationJanuary 19, 2021Pompura SL, Wagner A, Kitz A, LaPerche J, Yosef N, Dominguez-Villar M, Hafler DA.were solubilized by DMSO and conjugated in 250 μM BSA–lipid-free RPMI (Gibco, Thermo Fisher Scientific, 27016021) containing oleic acid (MilliporeSigma, O1008), arachidonic acid (MilliporeSigma, A3611), elaidic acid

(17) Assessment of Intracellular Delivery Potential of Novel Sustainable Poly(δ-decalactone)-Based MicellesPharmaceuticsAugust 2, 2020Kuldeep Kumar Bansal, Ezgi Özliseli, Gaurav Kumar Saraogi, Jessica M. Rosenholmwere precultured for 24 h with Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Cat. No. 27016021) containing no folic acid and supplements mentioned above to induce the overexpression of the folate

(18) Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemiaPloS oneJanuary 1, 2020Driehuis E, Oosterom N, Heil SG, Muller IB, Lin M, Kolders S, Jansen G, de Jonge R, Pieters R, Clevers H, et al.acid usually present in regular media. We used RPMI 1640 without folic acid (Thermofisher, cat.no. 27016021) supplemented with the same supplements as in organoid medium supplemented with 5 nM folinic acid (Sigma-Aldrich,

(19) Characterization of Human Mucosal-associated Invariant T (MAIT) CellsCurrent protocols in immunologyDecember 1, 2019Souter MNT, Loh L, Li S, Meehan BS, Gherardin NA, Godfrey DI, Rossjohn J, Fairlie DP, Kedzierska K, Pellicci DG, et al.RF10 media per Appendix, Maintenance of cell lines, but using folate free RPMI medium, Gibco cat. no. 27016021) 96-well u-bottom plate (TPP, cat. no. 92697) Stock of PMA (Sigma, cat. no. P1585): Reconstitute stock

(20) A microfluidic approach for sequential assembly of siRNA polyplexes with a defined structure-activity relationshipPeerJ Materials ScienceOctober 15, 2019Dominik M. Loy, Philipp M. Klein, Rafał Krzysztoń, Ulrich Lächelt, Joachim O. Rädler, Ernst Wagner(Biochrom), GibcoTM fetal bovine serum, (FBS, Thermo), RPMI 1640 (R2405500ML, Sigma), RPMI 1640, folate free (27016021, Thermo), 10× trypsin/EDTA in PBS (Biochrom), collagen A, 0.1% in HCl, one mg/ml (Biochrom), heparin

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RPMI 1640 Medium, no folic acid from Thermo Fisher Scientific

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Thermo Fisher Scientific

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RPMI 1640 Medium, no folic acid from Thermo Fisher Scientific

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