RPMI 1640 Medium, GlutaMAX Supplement, HEPES from Thermo Fisher Scientific

Product Specs
ItemRPMI 1640 Medium, GlutaMAX Supplement, HEPES
CompanyThermo Fisher Scientific
Price $354.00Get Pricing Get Quote for this Product
Catalog Number72400120
Quantity500 ml
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals
TypeMedium

Specifications/Features

Contains:
Contains 25 mM HEPES buffer.

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its ParalogsJournal of Molecular BiologyApril 15, 2026Sofia Gotthold, Keile R. Hansen, Andrew N. Brown, Soham P. Chowdhury, Christine M. Joyce, Viraj Vinish, Sofie I. Rodriguez, Sambhav Jain, Hannah I. Ghasemi, Amanda C. Yoon, et al.(P/S; Gibco, Cat#15140122). K-562 cells (female; ATCC) were maintained in RPMI 1640 medium (Gibco, Cat#72400120) containing 10% FBS and 1% P/S. U2OS DR-GFP cells (female; provided by Jeremy Stark’s laboratory, City

(2) The Plasmodium falciparum PPCS is a unique heteromeric complex with prokaryote-like activity and is a target of pantothenate analogsSci AdvMarch 13, 2026Xiangning Liu, Ruitao Jin, Riyad Domingo, Timothy J. Kotzé, Jinming Guan, Anton R. Hamann, Annica Chu, Gene Hart-Smith, Christina Spry, Anna E. Sexton, et al.(typically Group O+, Rh+, 4% hematocrit) in RPMI 1640 medium (Thermo Fisher Scientific, catalog number 72400120) supplemented with 25 mM Hepes, 11 mM glucose, 200 μM hypoxanthine, gentamicin (24 μg/ml), and Albumax

(3) The FANCD2-FANCI heterodimer coordinates chromatin openness and cell cycle progression throughout DNA double-strand break repairCell ReportsJanuary 27, 2026Christine M. Joyce, Julien Bacal, Soham P. Chowdhury, Andrew N. Brown, Amy K. Wang, Carmen Cruz, Kameron Bains, Zachary N. Rodriguez, Nathan J. McCormick, Yaara Tzadikario, et al.with 10% FBS and 1% P/S. K-562 (female) cells were obtained from ATCC, and cultured in RPMI 1640 (Cat#72400120; Gibco) with 10% FBS and 1% P/S. U2OS DR-GFP (female) cells (a gift from Jeremy Stark’s lab, City of

(4) Biomimetic fractal topography enhances podocyte maturation in vitroNat CommunDecember 15, 2025Chuan Liu, Praful Aggarwal, Karl T. Wagner, Shira Landau, Teng Cui, Xin Song, Laleh Shamaei, Naimeh Rafatian, Yimu Zhao, Sonia Rodriguez-Ramirez, et al.(Thermo Scientific, PMC4031) supplemented in fresh RPMI 1640 basal media containing HEPES and GlutaMAX (Gibco, #72400120) with 10% FBS and 1% pen-strep. To induce differentiation, cells were cultured under the differentiation

(5) CD47 inhibits phagocytosis through Vav dephosphorylationJournal of Cell BiologyDecember 1, 2025Wyatt D. Miller, Andrew Manion, Abhinava K. Mishra, Connor J. Sheedy, Annalise Bond, Brooke M. Gardner, Denise J. Montell, Meghan A. Morrisseyconditions. Macrophage progenitors were differentiated for seven days in RPMI-1640 (GIBCO, Catalog # 72400120) supplemented with 10% heat-inactivated fetal bovine serum, 1% Pen-Strep-Glutamine, and 20% L929-conditioned

(6) Target cell adhesion limits macrophage phagocytosis and promotes trogocytosisJournal of Cell BiologyNovember 3, 2025Kirstin R. Rollins, Sareen Fiaz, Ishwaree Datta, Meghan A. Morrisseycultured in IMDM (Fisher 12440061). Raji, and Jurkat cells were cultured in RPMI with 1% Glutimax (Fisher 72400120), 10% FBS, 1% PSG. Cells were routinely tested for mycoplasma. Cells were used for <20 passages. Lentivirus

(7) PfATP2 is a flippase on the Plasmodium falciparum surface that is important for growth and influences parasite sensitivity to antiplasmodial compoundsPLOS PathogensOctober 27, 2025Deyun Qiu, Eleanor England, Adele M. Lehanehaematocrit) in RPMI 1640 medium containing 25 mM HEPES and GlutaMAX (ThermoFisher Scientific Cat. # 72400120), and supplemented with 11 mM (additional) glucose, 200 μM hypoxanthine, 24 μg/mL gentamicin and 6 g/L

(8) SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its ParalogsbioRxivSeptember 16, 2025Sofia Gotthold, Keile R. Hansen, Andrew N. Brown, Soham P. Chowdhury, Hannah I. Ghasemi, Amanda C. Yoon, Christine M. Joyce, Julien Bacal, Brooke M. Gardner, Chris D. Richardson(P/S; Gibco, Cat#15140122). K-562 cells (female; ATCC) were maintained in RPMI 1640 medium (Gibco, Cat#72400120) containing 10% FBS and 1% P/S. U2OS DR-GFP cells (female; provided by Jeremy Stark’s laboratory, City

(9) Thiazole substitution of a labile amide bond—a new option toward antiplasmodial pantothenamide-mimicsAntimicrobial Agents and ChemotherapySeptember 3, 2025Xiangning Liu, Annica Chu, Mina Nekouei, Chunling Blue Lan, Alexandre Pierret, Karine Auclair, Kevin J. Saliba(typically Group O+, Rh+, 4% hematocrit) in RPMI-1640 medium (Thermo Fisher Scientific, catalogue number 72400120) supplemented with 25 mM HEPES, 11 mM glucose, 200 µM hypoxanthine, 24 µg/mL gentamicin and 6 g/L Albumax

(10) Single-cell transcriptomic landscape of the neuroimmune compartment in amyotrophic lateral sclerosis brain and spinal cordActa NeuropatholJuly 29, 2025John F. Tuddenham, Masashi Fujita, Emily Lee, Nivedita Nimmagadda, Anthony Khairallah, Claire Harbison, Xena E. Flowers, Guillermo Coronas-Samano, Silas Maniatis, Aidan Daly, et al.spun down at 300 rcf for 10 min. The pellet was resuspended in 2 ml staining buffer (RPMI (Fisher, 72400120) containing 1% B27) per 0.5 g of initial tissue and incubated with anti-myelin magnetic beads (Miltenyi,

(11) Aberrant Mitochondrial Metabolism in Alzheimer's Disease Links Energy Stress with FerroptosisAdvanced ScienceJuly 8, 2025Francesca Alves, Darius Lane, Adam Wahida, Md. Jakaria, Pawel Kalinowski, Adam Southon, Abdel Ali Belaidi, Teresa Samperi‐Esteve, Triet Phu Minh Nguyen, Peng Lei, et al.cells, derived from mouse hippocampus (Cat#SCC129, Merck), were cultured in RPMI 1640 growth media (Cat#72400120, Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Bovogen biologicals), penicillin and streptomycin

(12) A factor-based analysis of individual human microglia uncovers regulators of an Alzheimer-related transcriptional signaturebioRxivMarch 31, 2025Victoria S. Marshe, John F. Tuddenham, Kevin Chen, Rebecca Chiu, Verena C. Haage, Yiyi Ma, Annie J. Lee, Neil A. Shneider, Julian P. Agin-Liebes, Roy N. Alcalay, et al.spun down at 300 RCF for 10 mins. The pellet was resuspended in 2 mL staining buffer (RPMI (Fisher, 72400120) containing 1% B27) per 0.5 g of initial tissue and incubated with anti-myelin magnetic beads (Miltenyi,

(13) Intradermal delivery of SARS-CoV-2 RBD3-Fc mRNA vaccines via a needle-free injection system induces robust immune responses in ratsFrontiers in ImmunologyFebruary 17, 2025Cenrong Wang, Xin Tang, Chenghan Jiang, Yu Zhang, Bo Han, Yi Sun, Jianfeng Guo, Hanyu Peng, Zihan Wang, Yipeng Wang, et al.aseptically extracted 4 weeks after the boost (n = 3). The spleens were washed with RPMI 1640 medium (Gibco, 72400120) and lysed with an ammonium-chloride-potassium (ACK) lysis buffer (Leagene, China; CS0001). Complete

(14) Target cell adhesion limits macrophage phagocytosis and promotes trogocytosisbioRxivFebruary 8, 2025Kirstin Rollins, Sareen Fiaz, Meghan Morrisseywere cultured in IMDM (Fisher 12440061). Raji cells were cultured in RPMI with 1% Glutimax (Fisher 72400120), 10% FBS, 1% PSG. Cells were routinely tested for mycoplasma. Cells were used for <20 passages. Lentivirus

(15) High‐Throughput Single‐Nucleus RNA Profiling of Minimal Puncture FFPE Samples Reveals Spatiotemporal Heterogeneity of CancerAdvanced ScienceJanuary 1, 2025Weiqin Jiang, Xiang Zhang, Ziye Xu, Qing Cheng, Xiaohan Li, Yuyi Zhu, Fangru Lu, Ling Dong, Linghui Zeng, Weixiang Zhong, et al.in digestion media consisting of Liberase DL (400 µg mL−1) and elastase (100 µg mL−1) in RPMI (Gibco 72400120). The samples were partially dissociated using a gentleMACS Dissociator, followed by a 30‐minute incubation

(16) A cross-disease resource of living human microglia identifies disease-enriched subsets and tool compounds recapitulating microglial statesNature NeuroscienceDecember 1, 2024John F. Tuddenham, Mariko Taga, Verena Haage, Victoria S. Marshe, Tina Roostaei, Charles White, Annie J. Lee, Masashi Fujita, Anthony Khairallah, Ya Zhang, et al.spun down at 300 rcf for 10 min. The pellet was resuspended in 2 ml staining buffer (RPMI (Fisher, 72400120) containing 1% B27) per 0.5 g of initial tissue and incubated with anti-myelin magnetic beads (Miltenyi,

(17) A biopsy-sized 3D skin model with a perifollicular vascular plexus enables studying immune cell trafficking in the skinBiofabricationOctober 1, 2024Krutav Rakesh Shah, Laura Garriga-Cerda, Alberto Pappalardo, Leila Sorrells, Hun Jin Jeong, Chang H Lee, Hasan Erbil AbaciHuman naïve and Th1 cells were cultured in T cell medium (RPMI media with GlutaMax and HEPES, Gibco #72400120 supplemented with 10% heat inactivated FBS, Gibco #16000069, 1% 100X MEM amino acids Gibco # 11130051,

(18) Rapid profiling of transcription factor–cofactor interaction networks reveals principles of epigenetic regulationNucleic Acids ResearchSeptember 23, 2024Melissa M Inge, Rebekah Miller, Heather Hook, David Bray, Jessica L Keenan, Rose Zhao, Thomas D Gilmore, Trevor SiggersJurkat cells were grown in suspension RPMI 1640 GlutaMAX media (Thermo Fisher Scientific, catalog # 72400120) with 10% heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific, catalog # 132903) and

(19) Discovery of antiplasmodial pyridine carboxamides and thiocarboxamidesInternational Journal for Parasitology: Drugs and Drug ResistanceAugust 1, 2024Alexa Redway, Christina Spry, Ainka Brown, Ursula Wiedemann, Imam Fathoni, Larnelle F. Garnie, Deyun Qiu, Timothy J. Egan, Adele M. Lehane, Yvette Jackson, et al.erythrocytes, suspended in HEPES- and Glutamax™-supplemented RPMI 1640 (Thermo Fisher Scientific, order number 72400120) to which 11 mM D-glucose, 200 μM hypoxanthine, 24 mg/L gentamicin and 0.6% (w/v) Albumax II were added.

(20) LncR-GAS5 decrease in adenine phosphoribosyltransferase expresssion via binding TAF1 to increase kidney damage created by CIHHeliyonJune 30, 2024Liu W, Liang W, Zhang C, Liu H, Li H, Zhou L, Zhou L.(HK-2), purchased from Shanghai Cell Bank of Chinese Academy of Sciences, were cultured in RPMI1640 (72400120, Gibco, New York, USA) with 10 % FBS (16140071, Gibco) at 37 °C in an incubator supplemented with 5

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RPMI 1640 Medium, GlutaMAX Supplement, HEPES from Thermo Fisher Scientific

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Thermo Fisher Scientific

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RPMI 1640 Medium, GlutaMAX Supplement, HEPES from Thermo Fisher Scientific

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