Advanced RPMI 1640 Medium from Thermo Fisher Scientific

Product Specs
ItemAdvanced RPMI 1640 Medium
CompanyThermo Fisher Scientific
Price $350.00Get Pricing Get Quote for this Product
Catalog Number12633012
Quantity500 mL
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
TypeMedium

Specifications/Features

Contains:
Contains 2000 mg/L D-glucose non-essential amino acids (NEAA) and 110 mg/L sodium pyruvate but no L-glutamine.

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) The colloidal stability of albumin-based drug delivery systems has a profound effect on tumoricidal activityDrug DeliveryDecember 31, 2026Guojun Xiong, Chengwei Jiang, Andreas G. Schätzlein, Ijeoma F. Uchegbutissue culture flask with a vented cap using a complete medium composed of Advanced RPMI 1640 medium (#12633012, Thermo Fisher Scientific, UK) supplemented with heat-inactivated fetal bovine serum (FBS, 1% v/v, #F9665,

(2) Inhibition of STAT3-mediated glycolysis by bruceine D suppresses non-small-cell lung cancer progression in vitro and in vivoCancer Biol TherDecember 31, 2026Yu Zhu, Xuan Xu, Xiaofan Hong, Jiayi Zhang, Yi Zhou, Aofeng Sun, Yihan Ye, Yini Xu, Huanhai Xu, Haiyang Zhao, et al.cells, and SKMES-1 cells were grown in RPMI-1640 media (Thermo Fisher Scientific, Waltham, MA, USA, 12633020), Dulbecco’s modified Eagle's medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA, 11965092)(PC-9

(3) Optimising the method to develop spheroids from MDA-MB-468 human triple negative breast cancer cellsMolecular Biology ReportsDecember 1, 2026Shaza M. Mohamedahmed, Muhamad Noor Alfarizal Kamarudin, Premdass Ramdas, Usha Sundralingam, Ammu K. Radhakrishnanand fluorescence microscopy. Methods Cell line Roswell Park Memorial Institute (RPMI) (GIBCO, Cat. No.12633012, USA), foetal bovine serum [FBS] (GIBCO, Cat. No.16000044, USA), N-2-hydroxyethylpiperazine-N-2-ethane

(4) Tumor-Associated Mast Cells Drive Immunosuppression and Resistance to Anti-PD-1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4-mediated Regulatory T-Cell RecruitmentJ Biochem Mol ToxicolOctober 20, 2026Shuai Yang, Chuang YangBead Sorting Kit (11301D, Thermo Fisher Scientific). These cells were cultured in RPMI‐1640 medium (12633020, Gibco) supplemented with recombinant human SCF (20 ng/mL, 100‐1712, StemCell Technologies), IL‐3 (20

(5) VWA2 fuels colon cancer growth and immune evasion through Wnt/β-catenin signalingiScienceOctober 16, 2026Yanlin He, Jiang Chen, Huaqian Dong, Xiuya Ren, Tian Tian, Wei Xie, Tao Yangand recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, RPMI 1640,Gibco,Cat#12633020 DMEM,Gibco,Cat#11965118 Penstrep,Gibco,Cat#15140163 Amphotericin B,Gibco,Cat#15290026 Trypsin-0.05%

(6) Targeting the lactate/P300/H3K18la epigenetic pathway with a mechanoadaptive biomimetic hydrogel to suppress traumatic tendon heterotopic ossificationBioact MaterOctober 14, 2026Haoyang Feng, Kai Ang, Zengfa Deng, Chonghe Cui, Jialan Chen, Nanfeng Huang, Yimei Feng, Jimei Zhang, Danlin Li, Zhen Kong, et al.Gibco, USA) supplemented with 10% FBS and 1% ITS. Macrophages underwent maintenance in RPMI 1640 medium (12633012, Gibco, USA) supplemented with 10% FBS and 50 ng/mL M-CSF. Fibroblast cultivation utilized high-glucose

(7) One-Step Induction of Oogonia-Like and Pro-Spermatogonia-Like Cells From Porcine Pluripotent Stem CellsAdv Sci (Weinh)October 11, 2026Pengcheng He, Liang Zhong, Tianzhi Chen, Yaxuan Yan, Xinze Chen, Runbo Li, Yingjie Wang, Yadi Teng, Minglei Zhi, Meng Wang, et al.induction medium (aRB27 medium which is composed of Advanced RPMI 1640 Medium (Thermo Fisher Scientific, 12633012) supplemented with 1% B27 supplement, 0.1 mm NEAA, 1% penicillin‐streptomycin, 1% GlutaMAX supplemented

(8) Oncolytic adenovirus-mediated delivery of anti-CLDN18.2 IgG3 for the treatment of solid tumorsJ Immunother CancerOctober 9, 2026Lei Pei, Wanrong Zhang, Haoran Zhu, Xin Xu, Qingguo Guo, Yuan Meng, Wei Pang, Yifan Zhang, Wanyang Zeng, Mingrui Ma, et al.maintained in Dulbecco’s Modified Eagle Medium (#12491015; Gibco) or Roswell Park Memorial Institute 1640 (#12633020; Gibco) supplemented with 10% fetal bovine serum (DT500S; DearyTech), and 1% penicillin-streptomycin

(9) 53L10 T-cell engager tribody for immunotherapy of multiple solid tumorsFront ImmunolOctober 6, 2026Margherita Passariello, Martina Belardo, Toshikazu Inoue, Kentaro Kajiwara, Rosa Rapuano Lembo, Shu-Ichi Hashimoto, Claudia De LorenzoNCI-N87, PANC-1, and SKOV-3 cell lines were cultured in Roswell Park Memorial Institute 1640 Medium (12633012, RPMI 1640, Gibco, Life Technologies, Paisley, UK). The media were supplemented with 10% (vol/vol) heat-inactivated

(10) Tonicity drives collecting duct maturation in the mammalian kidneyNat CommunOctober 2, 2026Daisuke Inoue, Koichiro Miike, Shunsuke Tanigawa, Kei-Ichiro Yasunaga, Rae Maeda, Tomoko Ohmori, Sayoko Fujimura, Masashi Mukoyama, Tomomi Kamba, Jose Aramburu, et al.hormone-based protocol, from Day 6, CD organoids were cultured in advanced RPMI 1640 Medium (Gibco #12633012) supplemented with 1% GlutaMax, 0.1 µM RA, 100 ng/mL human R-spondin 1, 2 ng/mL human GDNF, 100 ng/mL

(11) Gymconopin C exhibits anti-non-small cell lung cancer effect by regulating miR-6777-5p/ADRB2 pathway to promote mitophagyJournal of advanced researchSeptember 1, 2026Li X, Han M, Zhang L, Xie X, Li C, Zhang H, An J, Yang J, Pu S, Duan Y, et al.USA) in a humidified environment with 5 % CO2 at 37°C. NCI-H1299 cells were grown in RPMI-1640 medium (12633020, Gibco, USA). Cell counting kit-8 assay The cells were digested and seeded into 96-well plates at

(12) Outer membrane vesicles from Fusobacterium necrophorum elicit protective immunity in mice and robust immune responses in sheepVirulenceSeptember 1, 2026Pengyu Zhao, Jiancheng Jiang, Ning Yang, Rongqi Liu, Kai Jiang, Lan Bi, Xianjing He, Donghua Guowith culture medium. After red blood cell lysis, bone marrow cells were cultured in RPMI 1640 medium (12633020, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; A15-101, PAA) and recombinant

(13) Lacticaseibacillus paracasei LPB27: Effectively Enhances the Immune System by Improving the Intestinal EnvironmentFoodsAugust 27, 2026Shaoshi Zhang, Yueyi Guo, Jiangyan Zhao, Jian Zhao, Yu Guo, Wenjie Yanlyophilized powder with a live bacterial count of 1.0 × 1011 CFU/g. RPMI 1640 culture medium (catalog no. 12633020, Gibco, Waltham, MA, USA) and fetal bovine serum (catalog no. 10099-141, Gibco/Thermo Fisher, Waltham,

(14) Endometriosis-derived iPSCs reveal conserved stromal maturation and endocrine responsivenessSci AdvAugust 21, 2026Hannah McDowell, Shiyang Sun, Ross McNally, Cassandra Huerta, Huma Asif, Julia Yoon, Angel Alvarez, Sule Yildiz, K. Grace Foley, Christina Boots, et al.supernatant was discarded, and the remaining iPSC pellet was resuspended in Advanced RPMI 1640 (Gibco, #12633012) and plated onto Corning Matrigel hESC-Qualified Matrix-coated tissue culture plates at various densities

(15) Laminin β4 is required for the development of human peripheral sensory neuronsDevelopmentAugust 15, 2026Kenyi Saito-Diaz, Tripti Saini, Archie Patel, Bhavik Dalal, Jayden Jackson, Christina James, Kimata Safi Thomas, Trinity Knight, Stephanie Beatrice Gogita, Kosuke Funato, et al.with Accutase for 20 min and seeded at a density of 100,000 cells/cm2 in RPMI medium (ThermoFisher, 12633012) with Glutamax (ThermoFisher, 35050061) and 100 ng/ml Activin A (R&D Systems, 338-AC-010). Cells were

(16) VAMP3 vesicles support host protein recruitment and Plasmodium liver stage developmentmBioAugust 12, 2026Chirgwin ME, Gan C, Godinez-Macias KP, Choe K, Shiau JC, Calla J, Kyle DE, Winzeler EA, Derbyshire ER.Resources (MRA-667, contributed by Mark F. Wiser). Cells were cultured in Advanced RPMI 1640 (Gibco, 12633012) with 4 mM L-glutamine (Gibco, 25030081) and gentamicin (50 μg/mL) supplementation. Hybridoma cells

(17) Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergyJCI InsightAugust 10, 2026Laila M. Rad, Michael N. Saunders, Laura A. Williams, Katarzyna W. Janczak, Chris L. Dorsett, Kate V. Griffin, Elizabeth J. Bealer, Jeffrey A. Ma, Sayre A. Tillery, Jyotirmoy Roy, et al.penicillin and 100 μg/mL streptomycin, and 100 μM 2-mercaptoethanol in advanced RPMI 1640 (Invitrogen, 12633012) culture medium. Intracellular cytokine staining was done using Cyto-Fast Fix/Perm buffer set (BioLegend,

(18) IGSF10 regulates osteogenesis and osteoclastogenesis via a noncanonical EGFR-STAT1 signaling axis to promote skeletal regenerationBone ResAugust 4, 2026Jin Wen, Yuwei Deng, Ruixue Jiang, Longwei Hu, Qianju Wu, Xiao Wang, Zheting Liao, Wenjing Ding, Fei Jiang, Xi Chen, et al.monocytic cell line (Sunncell, SNL-044) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA, 12633020) containing 10% FBS and 1% penicillin-streptomycin. All cells were maintained at 37 °C in a humidified

(19) Ligand-based directed differentiation to produce granulosa-like cells expressing steroidogenic enzyme genesStem Cell ResearchAugust 1, 2026Hana Kubo, Lina Lu, Maya VanZanten, Janavi Thyagraj, Diane C. Saunders, Ruli Gao, Monica M. Larondadifferentiated into the intermediate mesoderm lineage using Advanced RPMI 1640 Medium (Thermo Scientific, #12633012) supplemented with 1x Glutamax (Thermo Scientific, # 35050061) and 8 μM GSK-3 Inhibitor XVI CHIR99021

(20) The Legionella pneumophila effector SidL is an adenylyltransferase that modifies the glycolytic intermediate 3-phosphoglycerateMol CellJuly 24, 2026Joshua J. Black, A. Maxwell Burroughs, Edrees H. Rashan, Charles R. Nosal, Katerina A. Romanov, Marco A. Catipovic, Catherine Valadez, L. Aravind, Kevin G. Hicks, Caren L. Freel Meyers, et al.Biological Materials #G238). THP-1 cells (ATCC #TIB-202) were cultured in Advanced RPMI 1640 medium (Gibco #12633012) supplemented with 10% heat-inactivated FBS and 2 mM L-glutamine (Gibco #25030081) at 37°C in a 5% CO2

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Advanced RPMI 1640 Medium from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Advanced RPMI 1640 Medium from Thermo Fisher Scientific

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