RNase ONE Ribonuclease from Promega

Product Specs
ItemRNase ONE Ribonuclease
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM4261
Quantity1,000u
Concentration5–10u/µl
Molecular Weight~68kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to completely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2µg/µl RNA, 0.05% NP-40 and 2µg/µl BSA.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sequestration of growth cone surface proteins by cytoplasmic Lrrtm2 induces de novo amygdala innervation by cerebral cortex associative neuronsbioRxivApril 12, 2026Dustin E. Tillman, Omer Durak, Priya Veeraraghavan, John E. Froberg, Garrett Wheeler, Bogdan Budnik, Jeffrey D. Mackliswas rocked at 4 °C for 90 min with 1 μl 20% Triton (Sigma X100) and/or 1 μl 20% RNase ONE (Promega # M4261) or nothing. RNA was extracted from all samples (Zymo #R1050), and analyzed via TapeStation (Agilent

(2) Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategyMaterials Today BioApril 1, 2026Maojin Tian, Xiangwei Meng, Weiwei Song, Zhengyu Wu, Yang Liu, Xianing Dong, Shuai Ren, Juan Du, Peiqing ZhaoiRGD-NPs (si-FN1) to protect si-FN1 from enzymatic degradation was measured using RNase ONE™ Ribonuclease (M4261, Promega, Madison, USA). iRGD-NPs (si-FN1) were resuspended in 1 mL reaction buffer (ribonuclease-free).

(3) Targeted inhibition of RGS19 alleviates renal fibrosis by restoring autophagy and modulating immune cell infiltrationJournal of NanobiotechnologyFebruary 28, 2026Xinhao Niu, Yufeng Zhao, Long Li, Xiaoqing Xu, Cuidi Xu, Jiaheng Wu, Xiaohan Yu, Yanbo Chen, Ruiming Rong, Bin XuBriefly, free si-RGS19 and RDYH58-NPs@si-RGS19 NPs were incubated with RNase ONE™ ribonuclease (10 µg/mL; M4261, Promega, USA) at 37 °C for 0, 15, 30, 60, 120, or 240 min. After incubation, the NPs were collected

(4) Spliceosome inhibition induces Z-RNA and ZBP1-driven cell death in small cell lung cancerCell ReportsOctober 28, 2025Xinpei Jiang, Xueying Ma, Yunyun Zhou, Xiaodan Liu, Ting Zhang, William Kim, Siddharth Balachandran, Israel Cañadastransferred to a new Eppendorf tube, and the cell lysates were treated with or without 5U RNase One (Promega, M4261) for 30 min at room temperature. Sample RNA supernatants were extracted using Trizol at a ratio of 1

(5) Global analysis of excitotoxicity-induced alterations in RNA structure and RNA-protein binding in neuronsiScienceJune 20, 2025Elena Alvarez-Periel, Marianne C. Kramer, Cagla Akay-Espinoza, Daniel P. Jackson, Julia A. Tasca, Benjamin A. Garcia, Brian D. Gregory, Kelly L. Jordan-SciuttoV1,"Purified, tested, and validated in the Gregory lab with Protein Labs",N/A RNase ONE,Promega,Cat#M4261 Protease inhibitor cocktail,Sigma Aldrich,Cat#P8340 RNaseOUT™ Recombinant Ribonuclease Inhibitor,Invitrogen,Cat#10777019 Urea,Sigma

(6) True length of diverse capped RNA sequencing (TLDR-seq): 5'-3'-end sequencing of capped RNAs regardless of 3'-end statusNucleic Acids ResApril 4, 2025Jamie Auxillos, Arnaud Stigliani, Christian Skov Vaagensø, William Garland, Adnan Muhammed Niazi, Eivind Valen, Torben Heick Jensen, Albin Sandelin(Beckman Coulter, #A63881). Of note, as in the nAnT-iCAGE method [23], we incorporated an RNAse I (Promega, #M4261) treatment after reverse transcription but prior to cap trapping to cleave single-stranded RNAs which

(7) Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencingSTAR ProtocFebruary 6, 2025Diane Delobel, Hiromi Nishiyori-Sueki, Ilaria Nisoli, Hideya Kawaji, Pauline Robbe, Piero Carninci, Hazuki Takahashi,Thermo Fisher Scientific,18427013 Agencourt RNAClean XP,Beckman Coulter Life Sciences,A63987 RNase One Ribonuclease,Promega,M4261 Dynabeads M-270 Streptavidin,Thermo Fisher Scientific,65305 Ribonuclease H (RNase H) (60 U/μL),"Takara

(8) Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissuesPloS oneJanuary 1, 2025Li Y, Polychronopoulou M, Boonen I, Fikatas A, Gryseels S, Laudisoit A, de Bellocq JG, Vrancken B, Magiorkinis G, Lemey P, et al.Millipore, Merck, Germany) and nuclease digestion was performed using 25U RNase One Ribonuclease (catalog #M4265, Promega, the Netherlands) and 30U of Benzonase Nuclease (catalog #E1014-25KU, Millipore, Merck, Germany)

(9) Viral metagenomic investigation of two Caribbean echinoderms, Diadema antillarum (Echinoidea) and Holothuria floridana (Holothuria)PeerJNovember 26, 2024Ian Hewson, Marilyn Brandt, Kayla Budd, Mya Breitbart, Christopher DeRito, Samuel Gittens Jr, Michael W. Henson, Alwin Hylkema, Moriah Sevier, Matthew Souza, et al.AM2238), 5U Benzonase nuclease (Sigma Aldrich; part number E1014) and 20U RNase ONE (Promega; part number M4261) for 2 hours at 37 °C to digest extracellular and non-ribosome-bound nucleic acids. Following incubation,

(10) CapTrap-seq: a platform-agnostic and quantitative approach for high-fidelity full-length RNA sequencingNature CommunicationsJune 27, 2024Sílvia Carbonell-Sala, Tamara Perteghella, Julien Lagarde, Hiromi Nishiyori, Emilio Palumbo, Carme Arnan, Hazuki Takahashi, Piero Carninci, Barbara Uszczynska-Ratajczak, Roderic GuigóXP beads, and resuspended in 42 μl of nuclease-free water. The RNase ONE Ribonuclease (catalog num. M4261, Promega) was used at this point to eliminate the Biotin groups at the 3′ end by degrading only the

(11) Higher convergence of human-great ape enteric eukaryotic viromes in central African forest than in a European zoo: a One Health analysisNature CommunicationsJune 21, 2023Victor Narat, Maud Salmona, Mamadou Kampo, Thibaut Heyer, Abdeljalil Senhaji Rachik, Severine Mercier-Delarue, Noémie Ranger, Stephanie Rupp, Philippe Ambata, Richard Njouom, et al.technologies, #DB0715K); Benzonase® Nuclease (MERCK Millipore, # 70664-3); RNAse One TM Ribonucleas (Promega, # M4261) for 30 min, at 37 °C. Total nucleic acids were extracted using NucliSENS®easyMAG® (Biomerieux,#280130-280135)

(12) CoLoC-seq probes the global topology of organelle transcriptomesNucleic acids researchFebruary 22, 2023Jeandard D, Smirnova A, Fasemore AM, Coudray L, Entelis N, Förstner KU, Tarassov I, Smirnov A.U/ml; Thermo Fisher Scientific, Waltham, USA, Cat # EN0551), RNase One (Promega, Madison, USA, Cat# M4261), RNase T1 (Thermo Fisher Scientific, Waltham, USA, Cat # EN0541), SUPERase·In RNase Inhibitor (Thermo

(13) Polysome-CAGE of TCL1-driven chronic lymphocytic leukemia revealed multiple N-terminally altered epigenetic regulators and a translation stress signatureeLifeAugust 8, 2022Ogran A, Havkin-Solomon T, Becker-Herman S, David K, Shachar I, Dikstein R.SP1100) and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega, cat. M4261) digestion to release cDNAs corresponding to the 5′ ends of the original mRNA. 5′ linkers harboring

(14) Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeastSTAR protocolsDecember 17, 2021Matia-González AM, Jabre I, Gerber AP.RNasin® Ribonuclease inhibitor,Promega,N2511 Polyadenylic acid potassium salt (Poly(A)),Sigma,P9403 RNase ONE,Promega,M4265 peqGREEN RNA/DNA dye,Peqlab,732–3196 Precision Plus Protein Dual Color Standards,BIO-RAD,1610374 Tris,Thermo Fisher

(15) Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150Nature CommunicationsDecember 1, 2021Yun-Tao Liu, David Strugatsky, Wei Liu, Z. Hong Zhouinstructions. Virions were processed directly with miRNeasy or were pretreated with RNase One (Promega M4261) to hydrolyze RNA molecules outside of the virion. Total RNA was eluted in 50 µl of nuclease-free water

(16) G3BP1 inhibits Cul3^SPOP to amplify AR signaling and promote prostate cancerNature communicationsNovember 18, 2021Mukhopadhyay C, Yang C, Xu L, Liu D, Wang Y, Huang D, Deonarine LD, Cyrta J, Davicioni E, Sboner A, et al.Lysates of G3BP1 and G3BP1RRM transfected cells were treated without or with 100 U RNase ONE (Promega #M4261) for 1 h at 37 °C. Efficient RNase ONE treatment was confirmed by running samples into Agilent bioanalyzer.

(17) Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processingMolecular CellApril 1, 2021Tassa Saldi, Kent Riemondy, Benjamin Erickson, David L. BentleyDNAse I,NEB,M0303 ProteoGuard,Takara,635673 Formaldehyde 16% methanol free,ThermoFisher,28908 RNAseVI,Ambion,N/A RNAse One,Promega,M4261 Model cell lines,, HEK293 Flp-in T-Rex pcDNA5 Rpb1 αAmr WT,Fong et al 2014,N/A HEK293 Flp-in T-Rex

(18) Spliceosome-targeted therapies trigger an antiviral immune response in triple-negative breast cancerCellJanuary 14, 2021Elizabeth A. Bowling et al.supernatant was transferred to a new Eppendorf. Lysates were treated with or without 5U RNaseOne (Promega, M4261) for 30 minutes at room temperature. RNA was harvested using Trizol. Chloroform was added at a ratio

(19) Exogenously overexpressed intronic long noncoding RNAs activate host gene expression by affecting histone modification in ArabidopsisScientific ReportsDecember 1, 2020Zhang-Wei Liu, Nan Zhao, Yin-Na Su, Shan-Shan Chen, Xin-Jian HeRNase-treated RT-qPCR, 10 µg of total RNA was digested with RNase III (NEB, M0245S) or RNase one (Promega, M4261) according to the instruction manual. After chloroform purification, 1 µg of RNA was treated with DNase

(20) Multiplex detection of miRNAs based on aggregation-induced emission luminogen encoded microspheres†RSC AdvancesDecember 2, 2019Dan Zou et al.R-Phycoerythrin Conjugate (SAPE, 1 mg mL−1) was purchased from Thermo Fisher. RNase ONE™ ribonuclease (M4265, 10 U μL−1) was purchased from Promega. Sample preparation Preparation of AIEgens-encoded microspheres AIEgens-encoded

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