Ribonuclease H from Promega

Product Specs
ItemRibonuclease H
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM4281
Quantity50u
Concentration0.5–2u/µl
Molecular Weight~67kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to produce 1nmol acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20µM radiolabeled poly(rA):poly(dT).

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A multiparametric screen uncovers FDA-approved small molecules that potentiate the nuclear mechano-dysfunctions in ATR-defective cellsScientific ReportsDecember 28, 2024Maria Rosaria Cera, Giulia Bastianello, Divya Purushothaman, Adrian Andronache, Flora Ascione, Michela Robusto, Giovanni Fagà, Maurizio Pasi, Giuseppe Meroni, Qingsen Li, et al.according to manufacturer instructions. cDNA samples were treated with 1 μL RNAse H (Promega, Cat# M4281) for 20 min at 37˙C and stored at − 80 °C. Gene expression analysis was performed by the qPCR-Service

(2) Cancer cell histone density links global histone acetylation, mitochondrial proteome and histone acetylase inhibitor sensitivityCommunications biologyAugust 27, 2022Bruhn C, Bastianello G, Foiani M.according to manufacturer instructions. cDNA samples were treated with 1 µL RNAse H (Promega, Cat# M4281) for 20 min at 37 degrees and stored at −80 °C. Gene expression analysis was performed by the qPCR-Service

(3) Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of KeratoconusDisease markersJanuary 1, 2022Navel V, Malecaze J, Belville C, Choltus H, Henrioux F, Dutheil F, Malecaze F, Chiambaretta F, Blanchon L, Sapin V.incubated at 65°C for 5 min, 50°C for 10 min, 80°C for 10 min, and, after addition of RNase H (Promega, M4281), 37°C for 20 min. The primer sequences used for classic or quantitative RT-PCR are indicated in Table

(4) Comparison of Subgenomic and Total RNA in SARS-CoV-2-Challenged Rhesus MacaquesJournal of VirologyMarch 25, 2021Gabriel Dagotto, Noe B. Mercado, David R. Martinez, Yixuan J. Hou, Joseph P. Nkolola, Robert H. Carnahan, James E. Crowe, Ralph S. Baric, Dan H. Barouchof infection, SARS-CoV-2 infectious viral particles were treated with 25 units of RNase H (Promega M4281) for 1 h at 37°C. Cells were then infected in triplicate wells at a 0.1 or 1.0 multiplicity of infection

(5) RTP4 Is a Potent IFN-Inducible Anti-flavivirus Effector Engaged in a Host-Virus Arms Race in Bats and Other MammalsCell host & microbeNovember 11, 2020Boys IN, Xu E, Mar KB, De La Cruz-Rivera PC, Eitson JL, Moon B, Schoggins JW.Recombinant Proteins" Universal Type I IFN,PBL,11200 SCR7 pyrazine,Tocris,5342 Ribonuclease H,Promega,M4281 Micrococcal Nuclease,NEB,M0247S RNasin Plus,Promega,N2615 DNase I,NEB,M0303S Random Hexamers,ThermoFisher,N8080127 Pierce Protein A Magnetic Beads

(6) Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phaseeLifeFebruary 15, 2019Sven Schenk, Stephanie C Bannister, Fritz J Sedlazeck, Dorothea Anrather, Bui Quang Minh, Andrea Bileck, Markus Hartl, Arndt von Haeseler, Christopher Gerner, Florian Raible, et al.Invitrogen, #18010–025), 0.073 U E. coli DNA ligase (Invitrogen, #18052–019), and 0.015 U RNase H (Promega, #M4281); 20 µL 5X second strand buffer (Invitrogen, #10812–014), in a final volume of 100 µL. Reactions were

(7) The Exon Junction Complex Undergoes a Compositional Switch that Alters mRNP Structure and Nonsense-Mediated mRNA Decay ActivityCell reportsNovember 27, 2018Mabin JW, Woodward LA, Patton RD, Yi Z, Jia M, Wysocki VH, Bundschuh R, Singh G.JetPrime transfection reagent,PolyPlus,114-01 oligo-(dT)15 Primer,Promega,C1101 Superscript III,Invitrogen,18080044 Ribonuclease H,Promega,M4281 2X SYBR Green Master Mix,Applied Biosystems,4309155 RNase I,Promega,M4261 Ribo-Zero rRNA Removal

(8) Spt6 Association with RNA Polymerase II Directs mRNA Turnover During TranscriptionMolecular CellJune 21, 2018Raghuvar Dronamraju, Austin J. Hepperla, Yoichiro Shibata, Alexander T. Adams, Terry Magnuson, Ian J. Davis, Brian D. StrahlA1757 Benomyl,SIGMA (Roche),Catalog # 45339 Hydroxyurea,SIGMA (Roche),Catalog # H8627 RNAseH,Promega,Catalog # M4281 T4 Polynucleotide Kinase,NEB,Catalog # M0201S ULTRAhyb Oligo Buffer,Thermo Fisher Scientific,Catalog

(9) T Lymphocytes Influence the Mineralization Process of BoneFrontiers in immunologyJanuary 1, 2017El Khassawna T, Serra A, Bucher CH, Petersen A, Schlundt C, Könnecke I, Malhan D, Wendler S, Schell H, Volk HD, et al.N2511; M-MLV Reverse Transcriptase RNase H Minus, Point Mutant, Promega, M3683; Ribonuclease H, Promega, M4285]. Primers were tested for the annealing temperature and efficiency prior to use: mCOL1a1 ase: 5′-gttccaggcaatccacgag-3′,

(10) Protocol for using signal peptide differential display and representational difference analysis to isolate differentially expressed cDNAs from fetal mouse brainBrain Research ProtocolsSeptember 15, 2000Annika Eriksson, Katarina Nordqvist

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Ribonuclease H

Ribonuclease H from Promega

Description

Specifications/Features

Promega

Citations (10)

Reviews

Ribonuclease H from Promega

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