RNase HII from New England Biolabs

Product Specs
ItemRNase HII
CompanyNew England Biolabs
Price $327.00 Inquire
Catalog NumberM0288L
Quantity1250 units
Concentration5,000 units/ml
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to yield a fluorescence signal consistent with the nicking of 100 picomol of synthetic double-stranded DNA substrate containing a single ribonucleotide near the quencher of a fluorophore/quencher pair in 30 minutes at 37°C in 1X ThermoPol Buffer.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Emergent ribozyme behaviors in oxychlorine brines indicate a unique niche for molecular evolution on MarsNature CommunicationsMay 20, 2024Tanner G. Hoog, Matthew R. Pawlak, Nathaniel J. Gaut, Gloria C. Baxter, Thomas A. Bethel, Katarzyna P. Adamala, Aaron E. Engelhartquantified using a 5′-fluorescein label and a 20% urea-PAGE gel. RNase HII (New England BioLabs Inc. Cat#M0288S) was assessed with the same protocol but in 1X ThermoPol Buffer (New England BioLabs Inc. Cat#B9004S). TaqI-v2

(2) CST–polymerase α-primase solves a second telomere end-replication problemNatureMarch 21, 2024Hiroyuki Takai, Valentina Aria, Pamela Borges, Joseph T. P. Yeeles, Titia de Langewere incubated for 45 min at 37°C in 1X ThermoPol buffer (NEB) with 15 units E. coli RNase HII (NEB #M0288). Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K

(3) Rapid and accurate detection of SARS-CoV-2 using the RHAM technologySci RepDecember 20, 2023Zhuo Xiao, Xiaoli Liu, Xiaolong Kang, Yaoheng Feng, Lijun Zheng, Chong Chenreaction RNase HII reporting system signal was verified by adding 5 U RNase HII nuclease (New England Biolabs, M0288), 5 pmol probe (6-FAM-dT or 6-FAM-dC is used as the quenching group and 3′-BHQ1 or BHQ1-dT as the fluorophore.),

(4) CRISPR/Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HIIScientific ReportsFebruary 15, 2023Shahin Eghbalsaied, Wilfried A. Kues(700 ng/ml) for 15 h were electrotransfected with a mixture of either of 0, 1, 5, or 10 µl RNase HII (#M0288, 5 units/µl, NEB) combined with 20 µg pSGD69/pSGD75 (2 µg/µl) and 2 million cells in 250 OptiMEM-GlutaMAX.

(5) Increased expression of PolÎ´ does not alter the canonical replication program in vivoWellcome Open ResearchMay 4, 2021RÃ³bert Zach, Antony M. Carrgenomic DNA containing increased quantities of misincorporated ribonucleotides treated by RNAse H2 (NEB, M0288S) and subsequently denatured at 95°C. Sequencing was Page 5 of 18 Wellcome Open Research 2021, 6:44

(6) Increased expression of Polδ does not alter the canonical replication program in vivoWellcome Open ResearchFebruary 26, 2021Róbert Zach, Antony M. Carrgenomic DNA containing increased quantities of misincorporated ribonucleotides treated by RNAse H2 (NEB, M0288S) and subsequently denatured at 95°C. Sequencing was Page 5 of 18 Wellcome Open Research 2021, 6:44

(7) Preparation and Analysis of GLOE-Seq Libraries for Genome-Wide Mapping of DNA Replication Patterns, Single-Strand Breaks, and LesionsSTAR ProtocolsOctober 28, 2020Giuseppe Petrosino, Nicola Zilio, Annie M. Sriramachandran, Helle D. UlrichFC-110-3002 RNase A from bovine pancreas,Sigma-Aldrich,Cat# 10109169001 RNase H,New England Biolabs,Cat# M0288S Sarkosyl,Sigma-Aldrich,Cat# 61743 Sodium chloride,Sigma-Aldrich,Cat# S3014 Sodium citrate,Sigma-Aldrich,Cat#

(8) Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 NucleaseMolecular cellJune 18, 2020Álvarez-Quilón A, Wojtaszek JL, Mathieu MC, Patel T, Appel CD, Hustedt N, Rossi SE, Wallace BD, Setiaputra D, Adam S, et al.nanobody resin,"(Schellenberg et al., 2018)",N/A Recombinant E. coli RNase HII,New England Biolabs,M0288S Recombinant human APE1,New England Biolabs,M0282S Recombinant human APE2 full-length,This paper,N/A Recombinant

(9) High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductaseNucleic acids researchMay 7, 2020Cerritelli SM, Iranzo J, Sharma S, Chabes A, Crouch RJ, Tollervey D, El Hage A.B9004S), either in absence or presence of 25 u of recombinant E. coli RNase HII (New England Biolabs M0288S), or in presence of both 25 u RNase HII and 0.1 μg μl−1 RNase A, in a total volume of 50 μl, at 37°C

(10) Large-Scale Photolithographic Synthesis of Chimeric DNA/RNA Hairpin Microarrays To Explore Sequence Specificity Landscapes of RNase HII CleavageBiochemistryNovember 5, 2019Jory Lietard, Masad J. Damha, Mark M. Somozawere incubated with a buffered solution of E. coli recombinant RNase HII (5 units, New England Biolabs M0288S) at 37 °C [10 mM KCl, 20 mM Tris-HCl, 10 mM (NH4)2SO4, 2 mM MgSO4, and 0.1% Triton X-100 (pH 8.8)],

(11) DNA Polymerase Delta Synthesizes Both Strands during Break-Induced ReplicationMolecular CellNovember 1, 2019Roberto A. Donnianni, Zhi-Xiong Zhou, Scott A. Lujan, Amr Al-Zain, Valerie Garcia, Eleanor Glancy, Adam B. Burkholder, Thomas A. Kunkel, Lorraine S. SymingtonBLU513H250 β Agarase I,NEB,Cat# M0392 Shrimp Alkaline Phosphatase,NEB,Cat# M0371 RNase HII,NEB,Cat# M0288 T4 RNA Ligase I,NEB,Cat# M0204 T7 DNA Polymerase,NEB,Cat# M0274 Zymolyase 20T,USBiological,Cat# 37340-57-1 Proteinase

(12) Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic DevelopmentCell reportsOctober 30, 2018Uehara R, Cerritelli SM, Hasin N, Sakhuja K, London M, Iranzo J, Chon H, Grinberg A, Crouch RJ.Peptides, and Recombinant Proteins",, Mouse RNase H2,This study,N/A E. coli RNase HII,New England Biolabs,M0288S T4 Polynucleotide Kinase,New England Biolabs,M0201S Phosphodiesterase I,Worthington Biochemical,LS003926 Streptavidin,Sigma-Aldrich,S4762 Pierce

(13) Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeqNature ProtocolsSeptember 1, 2015James Ding, Martin S. Taylor, Andrew P. Jackson, Martin A. M. ReijnsSequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described46) for emRiboSeq; other recombinant

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RNase HII

RNase HII from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (13)

Reviews

RNase HII from New England Biolabs

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