Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemPhosphatase Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price $285.00 Inquire
Catalog Number5870S
Quantity1 ml
Research AreaRelated Products
ApplicationsW
Clone100X

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stressCell Chemical BiologyMay 21, 2026Alex E. Ekvik, Megan M. Kober, Denis V. Titov"PTMScan Trypsin, TPCK-treated",Cell Signaling Technology,Cat#56296 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat#5870S Roche cOmplete protease inhibitor cocktail,Sigma-Aldrich,Cat#11836170001 X-tremeGENE™ 9 DNA Transfection Reagent

(2) Expanding the Global Map of Protein Post Translational Modifications with Immunoaffinity Enrichment and nDIA Analysis on the Orbitrap Astral Mass SpectrometerMolecular & Cellular ProteomicsApril 6, 2026Mukesh Kumar, Anthony P. Possemato, Barry M. Zee, Srikanth Subramanian, Jian Min Ren, Alissa J. Nelson, Bin Zhang, Sean D. Landry, Jeffrey C. Silva, Brett Larsen, et al.samples were resuspended in 8M urea, 20 mM HEPES pH 8.5, with 2X Phosphatase Inhibitor Cocktail (CST #5870). Resuspended samples were homogenized with a polytron probe homogenizer and sonicated 2 times for 30

(3) FGF2 Deficiency Modulates Early Microglial Responses Without Affecting Photoreceptor Survival in a Retinitis Pigmentosa Mouse ModelCellsApril 2, 2026Felia C. Haffelder, Nundehui Díaz-Lezama, Zeynep Okutan, Claudia Grothe, Susanne F. Kochinhibitor (Sigma #11697498001) and Phosphatase Inhibitor Cocktail (Cell Signaling, Danvers, MA, USA #5870) with a Branson Sonifier W-450D at 40% amplitude. Proteins (30 μg per sample) were separated by a gradient

(4) Autoinhibitory control of MLKL governs pseudokinase domain phosphorylation and oligomerization during necroptosisCell ReportsApril 1, 2026Michelle J. Pan, Derek W. Abbott208851 Okadaic acid,MedChemExpress,Cat# HY-N6785 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat# 5870 Pierce™ NEM (N-ethylmaleimide),Thermo Scientific,Cat# 23030 Protease Inhibitor Cocktail,Sigma-Aldrich,Cat#

(5) 5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisionsbioRxivMarch 31, 2026Alexis B Roberson, James Marks, Ruby Pitts, Bavavarshini Tamilselvam, Brian Grieb, William P Tansey, Sezen MeydanAldrich, 4693159001) and phosphatase inhibitor (Selleck Chemicals, B15001 or Cell Signaling Technologies, 5870) on ice for 10 minutes and clarified via centrifugation at 12,000 rpm for 10 minutes at 4°C. Total protein

(6) Sex-specific APOE4-dependent innate immunity regulates meningeal lymphatics, brain lipids, neuroinflammation, and cognitionNeuronMarch 26, 2026Nickoleta Delivanoglou, Kennedi T. Todd, Francisco Almeida, Shanon Rego, Amogh Changavi, Myriam Spajer, Virginia Estades Ayuso, Liliana M. Pinho-Correia, Guadalupe Sanchez, Sofia P. das Neves, et al.cocktail,Thermo Fisher Scientific,Cat#78430 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat#5870 Phenylmethylsulfonyl fluoride,Cell Signaling Technology,Cat#8553 Ethanol,DeconLabs,Cat#2701 Pierce

(7) Targeting Activated Pathways in Doxorubicin-Resistant TNBC Alters Signaling, Survival and EMT: A Double-Edged SwordInt J Mol SciMarch 19, 2026Irem Dogan Turacli, Sahika Cingir Koker, Kubra Paspal Eroglu, Banu YalcinNP-40, 10% SDS), protease inhibitor cocktail (CST, 5871S), and phosphatase inhibitor cocktail (CST, 5870S), proteins were extracted. The BCA method was used to evaluate the total protein concentration using

(8) Glycogen phosphorylase L confers metabolic flexibility in neutrophils to fight fungal infections in nutrient-deprived tissuesCell Host MicrobeMarch 18, 2026Wonseok Choi, De-Dong Li, Colin T. McLaughlin, Doureradjou Peroumal, Kiyoshi P. Shiomitsu, Gillian A. Moschetta, Hossein Rahimi, Shuxia Wang, Kiaan Biswas, Danielle Xie, et al.inhibitor cocktail,Roche,Cat# 11697498001 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat# 5870 VECTASTATIN ABC-HRP Kit,Vector Lab,Cat# P15-4001 DAB Substrate Kit,Vector Lab,Cat# S15-4100 GLUCATELL

(9) The Effect of Mechanical Loading on Mitophagy in Aged MyoblastsCellsMarch 15, 2026Evangelos Tolis, Eirini Chatzinikita, Athanasios Moustogiannis, Antonios Giannopoulos, Maria Maridaki, Michael Koutsilieris, Anastassios PhilippouMO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. The extracts underwent electrophoresis

(10) Adipocyte‐Derived Exosomal miR‐5099 Mitigates M1 Macrophage Polarization and Adipose Inflammation via c‐Met/NF‐κB Axis to Improve Metabolic HealthAdvanced ScienceFebruary 21, 2026Ping Tang, Lixin Tai, Hongxia Xu, Dongliang Zhu, Jiajia Li, Hao Feng, Jinghui Cui, Shuhai Lin, Li‐jun Di, Li Wang(Sigma–Aldrich, P8340). In the case of phosphorylated proteins, a phosphatase inhibitor cocktail (Cell Signaling, 5870) was additionally incorporated into the lysis buffer. The proteins were fractionated using 10% SDS‐PAGE

(11) Mechanistic Language Modeling and Oxygenated 3D Screening Reveal Berberine and Enzalutamide Synergy in Resistant Prostate CancerbioRxivJanuary 30, 2026Chih-Hui Lo, Katie Shi, Lina Kafadarian, Alexandra Bermudez, Johnny Diaz, Liam Edwards, Yunqi Hong, Ziyi Chen, Hyeonji Hwang, Weihong Yan, et al.fixed in 4% paraformaldehyde containing phosphatase inhibitor cocktail (Cell Signaling Technology, Cat# 5870S) for 30 min on ice, then permeabilized and blocked in PBS containing 2% donkey serum and 0.25% Triton

(12) Myo-Inositol modulates AKT signalling, mitochondrial protein expression and intracellular Ca²⁺dynamics in human dermal fibroblastsScientific ReportsJanuary 28, 2026Paola Zanfardino, Alessandro Amati, Domenico Iacobellis, Matilde Colella, Vittoria Petruzzellasupplemented with protease and phosphatase inhibitor cocktails (Cell Signaling Technology #5871 and #5870S). Lysates were sonicated on ice and centrifuged at 16,000 × g for 10 min at 4 °C. Protein concentration

(13) Antibody-lectin chimeras for glyco-immune checkpoint blockadeNature BiotechnologyDecember 16, 2025Jessica C. Stark, Melissa A. Gray, Itziar Ibarlucea-Benitez, Marta Lustig, Annalise Bond, Brian Cho, Ishika Govil, Tran Luu, Megan J. Priestley, Tim S. Veth, et al.flask) (Thermo Fisher, 89900) supplemented with phosphatase inhibitor (1:100; Cell Signaling Technology, 5870S) and HALT protease inhibitor (1:100; Thermo Fisher, 78429), per the manufacturer’s protocol. Macrophage

(14) Combined protection against UVB-induced photoaging by oleuropein, hydroxytyrosol, and verbascoside through modulation of inflammation, oxidative stress, and collagen homeostasisScientific ReportsNovember 20, 2025Jing Wang, Minjia Yuan, Qi Li, Chenye Shen, Xinyi Zhang, Cuicui Zhu, Qingqing Cen(Cell Signaling Technology, 5871#) and 100× phosphatase inhibitor cocktail (Cell Signaling Technology, 5870#). Lysates were centrifuged at 12,000 ×g for 15 min at 4 °C, and protein concentrations in the supernatants

(15) Oligoclonal tumor-specific CD8 T-cell revival and IRE1α/XBP1-GDF15-mediated immunosuppressive niches determine neoadjuvant chemoimmunotherapy efficacy in cervical cancerJournal for ImmunoTherapy of CancerNovember 19, 2025Guangxu Cao, Yuhan Wang, Huimin Zeng, Yong Zhi, Yi Guo, Mengting Xu, Yetian Ruan, Ying Wang, Yuhang Xiao, Jianqiao Lu, et al.protease inhibitor cocktail (MCE, cat No. HY-K0010) and phosphatase inhibitor cocktail (CST, cat No. 5870). Protein concentrations were quantified via a BCA assay (Beyotime, cat No. P0398M), and 20 µg of total

(16) Elevated DNA damage without signs of aging in the short-sleeping Mexican cavefishElifeNovember 14, 2025Evan Lloyd, Fanning Xia, Kinsley Moore, Carolina Zertuche Mery, Aakriti Rastogi, Robert A Kozol, Olga Kenzor, Wesley Warren, Lior Appelbaum, Rachel L Moran, et al.inhibitor (25 X stock, Sigma, #11873580001) and 1 X phosphatase inhibitor (100 X stock, Cell Signaling, #5870 S) in RIPA buffer (Thermo Fisher Scientific, #89900). Next, 100 μl lysis buffer was added to each well,

(17) Elevated DNA damage without signs of aging in the short-sleeping Mexican cavefisheLifeNovember 14, 2025Evan Lloyd, Fanning Xia, Kinsley Moore, Carolina Zertuche Mery, Aakriti Rastogi, Robert A Kozol, Olga Kenzor, Wesley Warren, Lior Appelbaum, Rachel L Moran, et al.inhibitor (25 X stock, Sigma, #11873580001) and 1 X phosphatase inhibitor (100 X stock, Cell Signaling, #5870 S) in RIPA buffer (Thermo Fisher Scientific, #89900). Next, 100 μl lysis buffer was added to each well,

(18) A new biological enhancement therapy for anterior cruciate ligament reconstruction: the preclinical proof of anterior cruciate ligament reconstruction with tendon graft reseeded with autologous anterior cruciate ligament-derived cellsStem Cell Research & TherapyNovember 14, 2025Jinsung Park, Hyunsoo Soh, Yoon-Kwan Jang, Young Seok Lee, Myung-Kyu Lee, Hyunsung Kim, Tae-Hwan Kim, Khalid Alkhelaifi, Tae-Jin Kim, Jin Kyu Leestandard procedures involving a 1× RIPA buffer containing a phosphatase inhibitor (Cell Signaling, 5870 S) and protease inhibitor (Calbiochem, 535140). Quantification of the extracted proteins was carried

(19) Aberrant coupling of glutamate and tyrosine kinase receptors enables neuronal control of brain-tumor growthNeuronNovember 5, 2025Corina Anastasaki, Rui Mu, Chloe M. Kernan, Xuanwei Li, Rasha Barakat, Joshua P. Koleske, Yunqing Gao, Olivia M. Cobb, Xinguo Lu, Charles G. Eberhart, et al.11074440001 Perampanel,Cayman Chemical Company,Cat# 23003 Phosphatase inhibitor cocktail,Cell Signaling Technologies,Cat# 5870S Phosphate buffered saline,Gibco,Cat# 10010023 PowerLoad,ThermoFisher,Cat# P10020 Probenecid,ThermoFisher,Cat#

(20) Mitochondrial damage drives T-cell immunometabolic paralysis after major surgeryEMBO Molecular MedicineNovember 3, 2025Simon Hirschberger, Martin B Müller, Hannah Mascolo, Melissa Seitz, Stefan Nibler, David Effinger, Kun Lu, Joscha Büch, Martin Bender, Tobias Kammerer, et al.(#9803, Cell Signaling Technology) supplemented with a protease and phosphatase inhibitor (#5871 | #5870, Cell Signaling Technology). Equivalent protein concentrations were loaded onto polyacrylamide stacking

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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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