Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemPhosphatase Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price $285.00 Inquire
Catalog Number5870S
Quantity1 ml
Research AreaRelated Products
ApplicationsW
Clone100X

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Quantitative profiling of basal and stress-induced ribosome collisionsbioRxivJuly 22, 2026James Marks, Pedro H Ayres-Galhardo, Bavavarshini Tamilselvam, Alexis Roberson, Ruby Pitts, Nicholas Guydosh, Sezen Meydanprotease inhibitor (Sigma Aldrich, 4693159001) and phosphatase inhibitor (Cell Signaling Technologies, 5870) on ice for 10 minutes and clarified via centrifugation at 12,000 rpm for 10 minutes at 4°C. Total protein

(2) β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic interventionCell Death DisJune 13, 2026Geetika Kumari, Amit Kumar, Rasmiranjan Muduli, Mayami Das, Prithwik Bhowmik, Biplab Singha, Ankita Namdeo, Criss Dcosta, Neerja Wadhwa, Jaswinder Singh Maras, et al.inhibitor cocktails and for phospho-antibody, phosphatase inhibitors cocktail (Cell Signaling Technology, 5870) was additionally used. After sonication, samples were heated at 95 °C, and an equal amount of proteins

(3) Multi‐Omics Reveals Early Pregnancy Placental Dysfunction Associated With Preterm and Term PreeclampsiaMedComm (2020)June 1, 2026Ellen Menkhorst, Guannan Yang, Yimiao Yu, Leilani L. Santos, Wei Zhou, Argyro Syngelaki, Swati Varshney, Nicholas A. Williamson, Kaori Koga, Daniel Lorber Rolnik, et al.RIPA buffer (#89900, Thermo) plus protease inhibitor (#539134, Merck Millipore) and phosphatase inhibitor (#5870S, Cell Signaling Technologies) by mechanical homogenization (QIAGEN TissueLyser; RIPA buffer). A total

(4) Tumor sialylation regulates G-CSF stability and promotes neutrophil-mediated immunosuppression in breast cancerNature CommunicationsMay 22, 2026Stefan Mereiter, Gustav Jonsson, Tiago Oliveira, Ingrid-Judith Garberis, Johannes Helm, Markus Abeln, Ann-Kristin Jochum, Wolfram Jochum, Max J. Kellner, Marek Feith, et al.mM NaCl (Merck, Cat#106404), pH 8, containing phosphatase inhibitors (Cell Signaling Technology, Cat#5870). Samples were kept on ice for 30 min before being sonicated using a water bath sonicator for 1 min

(5) Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stressCell Chemical BiologyMay 21, 2026Alex E. Ekvik, Megan M. Kober, Denis V. Titov"PTMScan Trypsin, TPCK-treated",Cell Signaling Technology,Cat#56296 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat#5870S Roche cOmplete protease inhibitor cocktail,Sigma-Aldrich,Cat#11836170001 X-tremeGENE™ 9 DNA Transfection Reagent

(6) Incidence of B-cell Malignancies in Patients with Lung Cancer Receiving PD-1 Blockade TherapyClin Cancer ResMay 19, 2026Toshifumi Ninomiya, Caiyang Fang, Hirofumi Hamano, Teruya Morinaga, Wenhao Zhou, Toshihiro Koyama, Sakura Miki, Li Zhu, Yusuke Naoi, Daisuke Ennishi, et al.(Cell Signaling Technology; cat. #5871), and phosphatase inhibitor cocktail (Cell Signaling Technology; cat. #5870). Equal amounts of proteins from whole-cell lysates were separated via SDS-PAGE and blotted onto a transfer

(7) Complexity of PEDF multifunctional signaling in retinal development and diseasesCommunications BiologyMay 9, 2026Zihao Yu, Mingliang Zhang, Changjie Ren, Xiaomin Zhang, Colin J. Barnstable, Joyce Tombran-Tink, Xiaorong Li(Solarbio, Cat. No.R0020) containing PMSF (Solarbio, Cat. No.P0100) and phosphatase inhibitor (CST, Cat. No.5870) for 20 minutes, and total protein concentrations were measured using Bicinchoninic Acid protein assay

(8) Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid – TGR5 pathwayMolecular MedicineMay 9, 2026Maria Stefania Spagnuolo, Natasha Petecca, Francesca De Palma, Antonio Dario Troise, Angela Di Porzio, Valentina Barrella, Anella Saggese, Sabrina De Pascale, Marina De Stefano, Andrea Scaloni, et al.containing 1% w/v Protease Inhibitor Cocktail and 1% w/v Phosphatase Inhibitor Cocktail (cat 5871 and cat 5870, respectively; Cell Signalling distributed by Euroclone, Milan, Italy). Homogenates were incubated (30

(9) Blood levels of D-aspartate oxidase, D-amino acid oxidase, serine racemase, and pLG72 are influenced by diagnoses of schizophrenia and autism spectrum disorderSchizophrenia (Heidelb)April 25, 2026Elisa Maffioli, Francesco Errico, Zoraide Motta, Raffaella di Vito, Joshua Grana, Elisa De Grandis, Silvia Boeri, Claudio Bruno, Maria Pia Riccio, Felice Iasevoli, et al.Triton X-100 (93426, Fluka), supplemented with complete protease- (11836153001, Roche) and phosphatase- (5870, Cell Signaling) inhibitors cocktails. Samples were subsequently centrifuged at 16,000 xg for 30 min

(10) Expanding the Global Map of Protein Post Translational Modifications with Immunoaffinity Enrichment and nDIA Analysis on the Orbitrap Astral Mass SpectrometerMolecular & Cellular ProteomicsApril 6, 2026Mukesh Kumar, Anthony P. Possemato, Barry M. Zee, Srikanth Subramanian, Jian Min Ren, Alissa J. Nelson, Bin Zhang, Sean D. Landry, Jeffrey C. Silva, Brett Larsen, et al.samples were resuspended in 8M urea, 20 mM HEPES pH 8.5, with 2X Phosphatase Inhibitor Cocktail (CST #5870). Resuspended samples were homogenized with a polytron probe homogenizer and sonicated 2 times for 30

(11) FGF2 Deficiency Modulates Early Microglial Responses Without Affecting Photoreceptor Survival in a Retinitis Pigmentosa Mouse ModelCellsApril 2, 2026Felia C. Haffelder, Nundehui Díaz-Lezama, Zeynep Okutan, Claudia Grothe, Susanne F. Kochinhibitor (Sigma #11697498001) and Phosphatase Inhibitor Cocktail (Cell Signaling, Danvers, MA, USA #5870) with a Branson Sonifier W-450D at 40% amplitude. Proteins (30 μg per sample) were separated by a gradient

(12) Autoinhibitory control of MLKL governs pseudokinase domain phosphorylation and oligomerization during necroptosisCell ReportsApril 1, 2026Michelle J. Pan, Derek W. Abbott208851 Okadaic acid,MedChemExpress,Cat# HY-N6785 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat# 5870 Pierce™ NEM (N-ethylmaleimide),Thermo Scientific,Cat# 23030 Protease Inhibitor Cocktail,Sigma-Aldrich,Cat#

(13) 5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisionsbioRxivMarch 31, 2026Alexis B Roberson, James Marks, Ruby Pitts, Bavavarshini Tamilselvam, Brian Grieb, William P Tansey, Sezen MeydanAldrich, 4693159001) and phosphatase inhibitor (Selleck Chemicals, B15001 or Cell Signaling Technologies, 5870) on ice for 10 minutes and clarified via centrifugation at 12,000 rpm for 10 minutes at 4°C. Total protein

(14) Sex-specific APOE4-dependent innate immunity regulates meningeal lymphatics, brain lipids, neuroinflammation, and cognitionNeuronMarch 26, 2026Nickoleta Delivanoglou, Kennedi T. Todd, Francisco Almeida, Shanon Rego, Amogh Changavi, Myriam Spajer, Virginia Estades Ayuso, Liliana M. Pinho-Correia, Guadalupe Sanchez, Sofia P. das Neves, et al.cocktail,Thermo Fisher Scientific,Cat#78430 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat#5870 Phenylmethylsulfonyl fluoride,Cell Signaling Technology,Cat#8553 Ethanol,DeconLabs,Cat#2701 Pierce

(15) Targeting Activated Pathways in Doxorubicin-Resistant TNBC Alters Signaling, Survival and EMT: A Double-Edged SwordInt J Mol SciMarch 19, 2026Irem Dogan Turacli, Sahika Cingir Koker, Kubra Paspal Eroglu, Banu YalcinNP-40, 10% SDS), protease inhibitor cocktail (CST, 5871S), and phosphatase inhibitor cocktail (CST, 5870S), proteins were extracted. The BCA method was used to evaluate the total protein concentration using

(16) Glycogen phosphorylase L confers metabolic flexibility in neutrophils to fight fungal infections in nutrient-deprived tissuesCell Host MicrobeMarch 18, 2026Wonseok Choi, De-Dong Li, Colin T. McLaughlin, Doureradjou Peroumal, Kiyoshi P. Shiomitsu, Gillian A. Moschetta, Hossein Rahimi, Shuxia Wang, Kiaan Biswas, Danielle Xie, et al.inhibitor cocktail,Roche,Cat# 11697498001 Phosphatase inhibitor cocktail,Cell Signaling Technology,Cat# 5870 VECTASTATIN ABC-HRP Kit,Vector Lab,Cat# P15-4001 DAB Substrate Kit,Vector Lab,Cat# S15-4100 GLUCATELL

(17) The Effect of Mechanical Loading on Mitophagy in Aged MyoblastsCellsMarch 15, 2026Evangelos Tolis, Eirini Chatzinikita, Athanasios Moustogiannis, Antonios Giannopoulos, Maria Maridaki, Michael Koutsilieris, Anastassios PhilippouMO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. The extracts underwent electrophoresis

(18) Adipocyte‐Derived Exosomal miR‐5099 Mitigates M1 Macrophage Polarization and Adipose Inflammation via c‐Met/NF‐κB Axis to Improve Metabolic HealthAdvanced ScienceFebruary 21, 2026Ping Tang, Lixin Tai, Hongxia Xu, Dongliang Zhu, Jiajia Li, Hao Feng, Jinghui Cui, Shuhai Lin, Li‐jun Di, Li Wang(Sigma–Aldrich, P8340). In the case of phosphorylated proteins, a phosphatase inhibitor cocktail (Cell Signaling, 5870) was additionally incorporated into the lysis buffer. The proteins were fractionated using 10% SDS‐PAGE

(19) ZIP3 is essential for the encephalitogenic function of pTh17 cells by regulating intracellular zinc levelsJournal of Trace Elements in Medicine and BiologyFebruary 1, 2026Dominik Drmić, Liwei Wang, Lucile Noyer, Li Zhong, Stefan Feskeprotease inhibitor cocktail (Sigma-Aldrich; P8340) and phosphatase inhibitor cocktail (Cell Signaling; 5870). Cell lysates were precleared by centrifugation at 16,000 x g for 10 min at 4 °C. Cleared lysates were

(20) Mechanistic Language Modeling and Oxygenated 3D Screening Reveal Berberine and Enzalutamide Synergy in Resistant Prostate CancerbioRxivJanuary 30, 2026Chih-Hui Lo, Katie Shi, Lina Kafadarian, Alexandra Bermudez, Johnny Diaz, Liam Edwards, Yunqi Hong, Ziyi Chen, Hyeonji Hwang, Weihong Yan, et al.fixed in 4% paraformaldehyde containing phosphatase inhibitor cocktail (Cell Signaling Technology, Cat# 5870S) for 30 min on ice, then permeabilized and blocked in PBS containing 2% donkey serum and 0.25% Triton

Be the First to Write a Review!

Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Cell Signaling Technology

Citations (177)

Reviews

Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map