Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemProtease Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price $149.00 Inquire
Catalog Number5871S
Quantity1 ml
Research AreaRelated Products
Clone100X
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Osteoblastic sclerostin loop3-LRP4 interaction required by sclerostin to inhibit bone formationBone ResApril 24, 2026Luyao Wang, Xiaohui Tao, Hewen Jiang, Shijian Ding, Ning Zhang, Xin Yang, Shenghang Wang, Yihao Zhang, Nanxi Li, Haitian Li, et al.RIPA Lysis and Extraction Buffer (Thermo Scientific, 89901) supplemented with Protease Inhibitor Cocktail (CST, 5871) and centrifuged at 4 000 r/min for 30 min at 4 °C and replaced with binding buffer (PBS buffer, 20

(2) Circ72688 Drives Breast Cancer Invasion and Metastasis via the miR-654-5p/ORAI2 AxisOncology ResearchApril 22, 2026Haojie Yang, Zicong Tan, Zihao Liu, Kang Chen, Ning Liufu, Fengtao Jion ice using RIPA buffer (P0013B, Beyotime, Shanghai, China) supplemented with protease inhibitors (5871S, Cell Signaling Technology, Massachusetts, USA) for 30 min. Lysates were centrifuged at high speed,

(3) Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancerNat CommunApril 15, 2026Yapeng Su, Chunmei Liu, Xiang Lu, Hui-Yu Chuang, Guideng Li, Shiqun Shao, Yan Kong, Jihoon W. Lee, Rachel H. Ng, Stephanie Wong, et al.1 mL ice-cold PBS supplemented with 1X protease inhibitor cocktail (Cell Signaling Technology, Cat# 5871) and centrifuged. The cell pellets were resuspended in a cell lysis buffer containing 50 mM Tris-HCl

(4) Transcriptomic Profiling Identifies TALAM1 and LINC00702 as HIV-1-Responsive lncRNAs in MicrogliaInt J Mol SciApril 4, 2026Victoria Rojas-Celis, Catalina Millan-Hidalgo, Izabela Mamede, Isidora Morales-Vejar, Isidora Pittet-Díaz, Diego Fernández-Rodríguez, Paulo P. Amaral, Helder I. Nakaya, Sebastián Reyes-Cerpa, Fernando Valiente-Echeverría, et al.TNF-α-stimulated, and HIV-1-infected microglia were resuspended in RIPA buffer (Cell Signaling, Danvers, MA, USA, #5871S). Protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific,

(5) Alzheimer’s disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuitsNeuronMarch 30, 2026Robert Ellingford, Samuel S. Harris, Marten Kehring, Rikesh M. Rajani, Francesca Kar Wey Lam, David Graykowski, Dorothea Bӧken, Lindsay A. Welikovitch, Anita Khasnavis, Rhiannon Laban, et al.Optics,Cat#36-429 LED UV Spot Lamp,Intertronics,Part#IUV101 Protease Inhibitor cocktail,Cell Signaling,Cat#5871S cOmplete ULTRA protease inhibitor cocktail,Roche,Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail,Roche,Cat#4906845001 Zirconium

(6) Vinorelbine enhances the efficacy of oncolytic vaccinia virus in a preclinical model of ovarian high-grade serous carcinomaMolecular Therapy OncologyMarch 19, 2026Stephanie Drymiotou, Christophe J. Queval, Katherine E. Tyson, Lesley A. Sheach, Antonio Postigo, Ilaria Dalla Rosa, Darren P. Ennis, Michael Howell, Iain A. McNeish, Michael Waycollected and lysed in 21 μL of PBS supplemented with protease/phosphatase inhibitors (Cell Signaling, #5871) and 5U benzonase nuclease (Millipore, #E1014). SDS was added at a final concentration of 1% followed

(7) Targeting Activated Pathways in Doxorubicin-Resistant TNBC Alters Signaling, Survival and EMT: A Double-Edged SwordInt J Mol SciMarch 19, 2026Irem Dogan Turacli, Sahika Cingir Koker, Kubra Paspal Eroglu, Banu Yalcinpellets and the newly made RIPA buffer (NaCl, TrisHCl, NP-40, 10% SDS), protease inhibitor cocktail (CST, 5871S), and phosphatase inhibitor cocktail (CST, 5870S), proteins were extracted. The BCA method was used

(8) The Effect of Mechanical Loading on Mitophagy in Aged MyoblastsCellsMarch 15, 2026Evangelos Tolis, Eirini Chatzinikita, Athanasios Moustogiannis, Antonios Giannopoulos, Maria Maridaki, Michael Koutsilieris, Anastassios Philippou(Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride

(9) Aberrant nuclear pore complex degradation contributes to neurodegeneration in VCP diseaseNeuronMarch 4, 2026Sandeep Kumar Dubey, Divya Chaubey, Chiseko Ikenaga, Wen-Wen Lin, Hugo J. Bellen, Thomas E. LloydSodium acrylate,Santa Cruz Biotechnology,Cat# SC-236893 Protease Inhibitor Cocktail,Cell Signaling Technology,Cat# 5871 DAPI,Thermo Fisher,Cat# P36966 Phalloidin,Invitrogen,Cat# A12381 Immun-Blot PVDF Membrane,Biorad,Cat#

(10) Loss of miR-29a/b1 cluster reprograms the tumor microenvironment and contributes to immunosuppression in lung cancerCancer Immunology ResearchFebruary 25, 2026Natalie K. Horvat, Myritney Saint-Cloud, Raihaanah Bint Abdullah Bint Abdullah Muslim, Yanhua Tian, B. Leticia Rodriguez, Margaret A. Hall, Alireza Labani-Motlagh, Lixia Diao, Jing Wang, Gregory B. Lesinski, et al.RIPA buffer (10x from Cell Signaling) containing protease cocktail at 1X (100x from Cell Signaling, #5871S) and phosphatase inhibitors at 1X (100X cocktails II and III from Sigma, # P5726 and P0044, respectively).

(11) Targeting oxidative stress and mitochondrial dysfunction via URG7 overexpression in an in vitro Parkinson’s disease neuronal modelScientific ReportsFebruary 19, 2026Ilaria Nigro, Rocchina Miglionico, Ludovica Lela, Luigi Milella, Faustino Bisaccia, Maria Francesca Armentanofrom Sigma Aldrich-Merck (Saint Louis, MO, USA). RIPA buffer (#9806) and Protease Inhibitor Cocktail (#5871) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Quick-RNA™ MiniPrep kit was

(12) Stiff to Soft: A Protein‐Based Buffer Layer for Improving the Long‐Term Performance of Microneedle SensorsAdvanced MaterialsFebruary 8, 2026Lihao Guo, Youbin Zheng, Shuxiang Xu, Bingbing Feng, Kan Wang, Rou Huang, Gandong Zhou, Chutong Liu, Rawan Omar, Danyao Qu, et al.RIPA buffer (Thermo Fisher, 89900), containing protease inhibitor cocktail (Cell Signaling Technology, 5871). Protein was separated on 10% SDS PAGE and transferred to 0.45 µm nitrocellulose membranes (Merck Millipore

(13) Temporal photoproximity labeling of ligand-activated EGFR neighborhoods using MultiMapNature Chemical BiologyFebruary 1, 2026Zhi Lin, Wayne Ngo, Yu-Ting Chou, Harry Wu, Katherine J. Susa, Young-wook Jun, Trever G. Bivona, Jennifer A. Doudna, James A. Wellssupplemented with protease inhibitor (protease inhibitor cocktail 100×; Cell Signaling Technology, 5871). After 15 min of incubation on ice, cells were sonicated for 15 s (5 s on, 5 s off, 20%). Cell lysates

(14) Varietal Differences in Kidney Beans Modulate Gut Microbiota and Inflammation During High-Fat Diet-Induced Obesity in Male MiceNutrientsJanuary 30, 2026Alexane F. Rodrigue, Bruna B. Pereira, Giorgio Freije, Allison Sweet, Laili Mahmoudian, Mahmoud Aly, Salma Mahmoodianfard, Lalit Kishore, Marie-Claude Audet, Marcos F. Minicucci, et al.Danvers, MA, USA; Cat. # 9806) supplemented with protease inhibitors (Cell Signaling Technology; Cat. # 5871) and PMSF (Cell Signaling Technology; Cat. # 8553) using a Bead Mill 24 Homogenizer (Fisherbrand™, Thermo

(15) Nutrient requirements of organ-specific metastasis in breast cancerNatureJanuary 29, 2026Keene L. Abbott, Sonu Subudhi, Raphael Ferreira, Yetiş Gültekin, Sophie C. Steinbuch, Muhammad Bin Munim, Diya L. Ramesh, Sophie E. Honeder, Ashwin S. Kumar, Michelle Wu, et al.washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation

(16) Myo-Inositol modulates AKT signalling, mitochondrial protein expression and intracellular Ca²⁺dynamics in human dermal fibroblastsScientific ReportsJanuary 28, 2026Paola Zanfardino, Alessandro Amati, Domenico Iacobellis, Matilde Colella, Vittoria Petruzzella(Thermo Fisher Scientific, 89900) supplemented with protease and phosphatase inhibitor cocktails (Cell Signaling Technology #5871 and #5870S). Lysates were sonicated on ice and centrifuged at 16,000 × g for 10 min at 4 °C. Protein

(17) Aromatic microbial metabolite hippuric acid enhances inflammatory responses in macrophages via TLR-MyD88 signaling and lipid remodelingCell ReportsJanuary 27, 2026Gauri Mirji, Sajad Ahmad Bhat, Mohamed El El Sayed, Sarah Kim Reiser, Siva Pushpa Gavara, Ying Ye, Taito Miyamoto, Wujuan Zhang, Peter Vogel, Joel Cassel, et al.10010334 Simvastatin,Cayman chemical,Cat# 10010344 Protease inhibitor cocktail,Cell signaling technology,Cat#5871S ACK lysis buffer,Quality Biological,Cat# 118-156-101 DNase I,Sigma,Cat# 10104159001 Collagenase IV,Sigma,Cat#

(18) Environmental enrichment and physical exercise prevent stress-induced social avoidance and blood-brain barrier alterations via Fgf2Nature CommunicationsJanuary 16, 2026Sam E. J. Paton, José L. Solano, Alice Cadoret, Adeline Collignon, Luisa Bandeira Binder, Béatrice Daigle, Laura Menegatti Bevilacqua, Émanuelle Richer, François Coulombe-Rozon, Laurence Dion-Albert, et al.(Cell Signaling Cat. No. 9803) supplemented with protease inhibitor cocktail (Cell Signaling Cat. No. 5871). Samples were sonicated in ice-cold water, centrifuged for 10 min (17,949 × g, 4 °C), and supernatant

(19) A TGFB2/TNF-induced in vitro model of proliferative vitreoretinopathy (PVR) using ARPE-19 cells confirms nicotinamide as an inhibitor of EMT and VEGFA secretionPLOS OneJanuary 13, 2026Yuqing Huang, Roland Meister, Migle Lindziute, Maximilian Binter, Jan Tode, Carsten Framme, Heiko FuchsLaemmli Sample Buffer (Bio-Rad #1610737) supplemented with a 1 × protease inhibitor cocktail (Cell Signaling #5871S). The lysates were mixed with 2-mercaptoethanol (Sigma-Aldrich #60-24-2), denatured at 95°C for 5 min,

(20) Binding of Glycyl-tRNA synthetase to Mengovirus RNA stimulates translationNucleic Acids ResearchJanuary 8, 2026Fabian Droß, Tim Gödert, Selena A Fuchshuber, Dimitar V Nachev, Dmitri E Andreev, Markus Fricke, Muriel Ritsch, Gesche K Gerresheim, Simeon Repp, Yannic Noe, et al.NaCl, 20 mM Tris HCl pH 7.5, 1.5 mM MgCl2, 0.5% Triton X100, Protease inhibitor (Cell Signalling # 5871S) and 1 mM DTT). The lysate was split into aliquots and stored at -80°C. The GARS binding assay was performed

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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