Fig 1: Aldh1l1 bridges the link between PGK1 and UNC5CL in the development of DKD(A) Aldh1l1 was identified to interact with PGK1.(B) Representative blots and quantitative analysis of Aldh1l1 (n = 4).(C–E) CoIP results showing the complex formation of PGK1/UNC5CL/Aldh1l1 in NG- or HG-incubated HK-2 cells (n = 4).(F–H) CoIP results showing the complex formation of PGK1/UNC5CL/Aldh1l1 in kidneys from control and T1D-DKD mice (n = 4).(I–K) CoIP results showing the complex formation of PGK1/UNC5CL/Aldh1l1 in kidneys from control and T2D-DKD mice (n = 4).(L) Effects of Aldh1l1 small interfering RNA (siRNA) on the complex formation of PGK1/UNC5CL (n = 4).(M and N) Effects of Aldh1l1 siRNA on the impact of PGK1 overexpression (OE) on the UNC5CL-mediated inflammation (n = 4).Data are represented as mean ± SD. Statistical significance was determined by unpaired Student’s t test.∗p < 0.05 vs. NG or control (Con). See also Figures S5 and S6.