ssRNA Ladder from New England Biolabs

Product Specs
ItemssRNA Ladder
CompanyNew England Biolabs
Price $78.00 Inquire
Catalog NumberN0362S
Range500bp-9000bp
Concentration500 µg/ml
Quantity25 gel lanes
ApplicationsFunctional Genomics
Nucleic Acid TypeRNA Ladder
Features (standardized)Unstained
Size Range500 - 9000 bp
TypeRNA Ladder
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol to annotate dendritic cell maturation types in vivo making use of lipid nanoparticle-based approachesSTAR ProtocMarch 20, 2026Karo Van Lil, Janne Swinnen, Greta R. Webb, Sofie Rennen, Victor Bosteels, Sandra Maréchal, Clint De Nolf, Jessica Vetters, Jiří Březina, Evelien Van De Velde, et al.Bioscience,Cat#NU-890S UltraPure Agarose,Invitrogen,Cat#16500-500 ssRNA ladder,New England Biolabs,Cat#N0362S GelRed,Biotium,Cat#41003 Fetal calf serum,Greiner,N/A Cholesterol,Sigma-Aldrich,Cat#C8667 Liberase

(2) Depurination of sarcin/ricin loop 25S rRNA is signaled through the small ribosomal subunit during translationRNADecember 1, 2025Tanya Prashar, Katalin A. Hudakaniline) was separated on a 1.5% formamide agarose gel along with a combined ssRNA ladder (NEB N0364S and N0362S). To assess RNA integrity following separation, the gel was stained with 10 µL of 10,000× SYBR gold

(3) Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly developmentNat CommunAugust 30, 2025Siran Tian, Hung Nguyen, Ziqing Ye, Silvi Rouskin, D. Thirumalai, Tatjana Trcekformamide-free loading dye (Thermo Fisher: R0611) was added. After loading all samples and the RNA ladders (NEB: N0362S) onto the gel, the gel was run for 2 hrs at 70 V and at 4 °C to separate RNA populations. The gel was

(4) RNA PolyA Tailing Assay to Qualitatively Analyze Circular RNA ManufacturingBIO-PROTOCOLMay 20, 2025Zih-Shiuan Chuang, Chan-I Su, Hsin-I Wang, Chia-Yi Yunumber: B0363S) 11. RNase R (Abcam, catalog number: ab286929) 12. ssRNA ladder (NEB, catalog number: N0362S) 13. TBE buffer (5×) (VWR, catalog number: J885-5L) 14. Adenosine 5'-triphosphate (ATP) (NEB, catalog

(5) Removal of dsRNA byproducts using affinity chromatographyMolecular Therapy Nucleic AcidsApril 29, 2025Nathaniel E. Clark, Mateusz Kozarski, Sinem Demirel Asci, Jurgen Van den Heuvel, Matt R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon, Senne Dillenin Figures 2 and 3. Samples were not heated prior to electrophoresis. dsRNA and ssRNA ladders (NEB N0362S and N0363S, respectively) were loaded on the gels. Precast 10% TBE gels were used in Figure 1B (Thermo Fisher Scientific

(6) NicOPURE: nickless RNA circularization and one-step purification with engineered group II introns and cyclizing UTRsNucleic acids researchApril 10, 2025Gao X, Chen K, Wang H.wells for performing gel electrophoresis at 100 V for 60 min. Briefly, 3 μl of ssRNA Ladder (NEB, Cat.: N0362S) was also loaded in a separate lane. Gels were visualized using the Bio-Rad ChemiDoc imaging system

(7) Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancerExp Hematol OncolMarch 12, 2025Jingsheng Cai, Zheng Liu, Shaoyi Chen, Jingwei Zhang, Haoran Li, Xun Wang, Feng Yang, Shaodong Wang, Xiao Li, Yun Li, et al.with 2 × loading buffer and subjected to electrophoresis at 120 V for 30 min. An ssRNA Ladder (NEB, N0362S) served as the standard. Bands were visualized under UV irradiation (Bltlux, GelView 6000Plus). Western blot (WB

(8) Chemical nucleases are a robust alternative for RNase H cleavage of human ribosomal RNAPLoS OneFebruary 24, 2025Hagen Wesseling, Dennis Krug, Marvin Wehrheim, Michael W. Göbel, Stefanie Kaiserperformed for 2 h at 100 V in a running chamber (Roth) at 4°C. As size reference, a ssRNA Ladder (NEB #N0362S) was used. After separation RNA was stained with GelRed (Roth #223C.1) and visualized using a ChemiDoc MP

(9) Lipid Nanoparticles With Fine‐Tuned Composition Show Enhanced Colon Targeting as a Platform for mRNA TherapeuticsAdvanced ScienceNovember 25, 2024Riccardo Rampado, Gonna Somu Naidu, Olga Karpov, Meir Goldsmith, Preeti Sharma, Assaf Ezra, Lior Stotsky, Dor Breier, Dan PeerNew England Biolabs) before being loaded in the gel. The gel was run in the presence of ssRNA Ladder (Cat# N0362S, New England Biolabs) at 100 V for 40 min before being imaged using a Syngene PXi image analysis system. Biocompatibility

(10) Efficient circularization of protein-encoding RNAs via a novel cis-splicing systemNucleic Acids ResearchSeptember 23, 2024Shaojun Qi, Huiming Wang, Guopeng Liu, Qianshan Qin, Peng Gao, Bo YingSystem (Invitrogen, Cat # G8300) with the E-Gel EX 1% - 2% program, utilizing ssRNA ladder (NEB, Cat # N0362S) as a reference standard. Bands were visualized under blue light transillumination. mRNA synthesis The

(11) A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioningNature communicationsAugust 4, 2024Su CI, Chuang ZS, Shie CT, Wang HI, Kao YT, Yu CY.ZGENEBIO) and 2 M formaldehyde (Cat#F1635, Sigma). RNA samples (100–200 ng) and markers (200 ng, Cat#N0362S, NEB) were denatured by mixing with an equal volume of RNA loading dye (Cat#B0363S, NEB), heating at

(12) Stoichiometric expression of mRNA polycistrons by eukaryotic ribosomes (SEMPER) for compact, ratio-tunable multi-gene expressionCell SystemsJuly 17, 2024Mengtong Duan, Ishaan Dev, Andrew Lu, Goar Ayrapetyan, Mei Yi You, Mikhail G. Shapirogel electrophoresis on a 1% agarose gel in Tris acetate EDTA (TAE) stained with SYBR Safe. ssRNA ladder (NEB #N0362S) was used to confirm band sizes. mRNA concentrations for each IVT mRNA were quantified using a Qubit Fluorometer

(13) Protocol for the development of mRNA lipid nanoparticle vaccines and analysis of immunization efficiency in miceSTAR protocolsJune 21, 2024Karekar N, Reid Cahn A, Morla-Folch J, Saffon A, Ward RW, Ananthanarayanan A, Teunissen AJP, Bhardwaj N, Vabret N.buffer,Zymo research,#E1010 dsRNA ladder,New England Biolabs,#N0363S ssRNA ladder,New England Biolabs,#N0362S "UltraPure ethidium bromide, 10 mg/mL",Invitrogen,# 15585011 "Trypan blue solution, 0.4%",Gibco,#

(14) Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotidesCommunications ChemistryApril 1, 2023Denis Hartmann, Michael J. Boothdenature the RNA. Samples were run against a low-range ssRNA ladder (NEB, N0364S) or ssRNA ladder (NEB, N0362S). UV illumination Samples were held in a PCR tube rack (StarLabs) over aluminium foil with open lids.

(15) One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporationSTAR ProtocolsMarch 1, 2023Donovan Flumens, Diana Campillo-Davo, Ibo Janssens, Gils Roex, Jorrit De Waele, Sébastien Anguille, Eva Lion1 kb Plus DNA Ladder,Thermo Fisher Scientific,Cat# 10488085 ssRNA ladder,New England Biolabs,Cat# N0362S Propidium iodide (1 mg/mL),Life Technologies,Cat# P3566 BD FACSFlow,BD Biosciences,Cat# 342003 ,, Critical

(16) Evolution of naturally arising SARS-CoV-2 defective interfering particlesCommunications biologyOctober 27, 2022Girgis S, Xu Z, Oikonomopoulos S, Fedorova AD, Tchesnokov EP, Gordon CJ, Schmeing TM, Götte M, Sonenberg N, Baranov PV, et al.measured by NanoDrop 1000 (Thermo Scientific). Cap 1 mRNA was analyzed alongside ssRNA ladder (NEB, # N0362S) on a 1% agarose-formaldehyde denaturing gel to confirm size and quality. Long-range (LR)-qPCRs To

(17) Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stressiScienceSeptember 16, 2022Ma J, Shao X, Geng F, Liang S, Yu C, Zhang R.Blocking Reagent,Roche,Cat#11096176001 CDP-Star,Roche,Cat#12041677001 Nylon Membranes,Millipore,Cat#11417240001 ssRNA ladder,NEB,Cat#N0362S EnGen® Spy Cas9 NLS,NEB,Cat#M0646 T7 RNA Polymerase,NEB,Cat#M0251 Torula RNA,Sigma-Aldrich,Cat#R5636 Paraformaldehyde,Sigma-Aldrich,Cat#P6148 Formaldehyde

(18) Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytesiScienceAugust 19, 2022Sarah E. Cabral, Jessica P. Otis, Kimberly L. Mowry,ThermoFisher Scientific,Cat#EO0381 SecureSeal imaging spacers,Grace Bio-labs,Cat#470352 ssRNA ladder,New England Biolabs,Cat#N0362S Super-Signal west pico plus chemiluminescent substrate,ThermoFisher Scientific,Cat#34580 ThermalSeal,Excel

(19) Field-deployable, rapid diagnostic testing of saliva for SARS-CoV-2Scientific ReportsDecember 1, 2021Shan Wei et al.e.g., 6 ng carrier DNA for 20µL lysis buffer), and ~ 9 ng/µL lysis buffer volume of carrier RNA (NEB, N0362S, ~ 250 ng/µL), 2µL RNase Inhibitor, Murine (NEB, M0314S/L), 15µL buffer TE pH 8.0 (Ambion AM9849), and

(20) Investigating Transcriptomic Induction of Resistance and/or Virulence in Listeria monocytogenes Cells Surviving Sublethal Antimicrobial ExposureFoodsOctober 8, 2021Eleni-Anna Kokkoni, Nikolaos Andritsos, Christina Sakarikou, Sofia Michailidou, Anagnostis Argiriou, Efstathios Giaouriselectrophoresis (1.5% w/v TBE agarose gel; 100 V for 30 min) to verify its integrity, using the ssRNA Ladder (N0362S, 500–9000 bp, New England BioLabs Inc., Ipswich, MA, USA) as the molecular weight marker. The rest of

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ssRNA Ladder

ssRNA Ladder from New England Biolabs

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New England Biolabs

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ssRNA Ladder from New England Biolabs

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