3-O-Me-m7G(5)ppp(5)G RNA Cap Structure Analog from New England Biolabs

Product Specs
Item3-O-Me-m7G(5)ppp(5)G RNA Cap Structure Analog
CompanyNew England Biolabs
Price $163.00 Inquire
Catalog NumberS1411S
Quantity1 µmol
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary CellsMicromachines (Basel)March 15, 2026Hyun Woo Sung, Soojung Claire HurAG (cat#N-7113, TriLink Biotechnologies, San Diego, CA, USA) or Anti-Reverse Cap Analog (ARCA) (cat# S1411S, New England Biolabs, Ipswich, MA, USA), substituting uridine entirely with N1-Methylpseudouridine-5′-Triphosphate

(2) Genetically tractable embryonic cell lines from sea urchins Lytechinus variegatus and Strongylocentrotus purpuratusCommun BiolOctober 14, 2025Kate R. Castellano, Carl J. Manner, Riss M. Kell, Reanna M. McAtee, Nicole M. Capozzi, Gregory A. Wray, Andrea G. Bodnar(New England Biolabs, E2070S) and capped with a 3′-O-Me-m7G(5’)ppp(5’)G RNA cap structure analog (New England Biolabs, S1411S) following manufacturer’s instructions (New England Biolabs, RNA Synthesis Protocol, E2070). mRNA was

(3) A capless hairpin-protected mRNA vaccine encoding the full-length Influenza A hemagglutinin protects mice against a lethal Influenza A infectionGene TherapyJuly 1, 2025Victor Solodushko, Jin H. Kim, Brian Foutyand 2C in Fig. 3) were capped with an Anti-Reverse Cap Analog (ARCA, New England Biolabs, Cat. No. S1411S) and were compared to their uncapped variants. Appropriate schematics of each vector are included with

(4) Branched chemically modified poly(A) tails enhance the translation capacity of mRNANature BiotechnologyFebruary 1, 2025Hongyu Chen, Dangliang Liu, Jianting Guo, Abhishek Aditham, Yiming Zhou, Jiakun Tian, Shuchen Luo, Jingyi Ren, Alvin Hsu, Jiahao Huang, et al.final concentration of 2 mM GTP and 8 mM 3′-O-Me-m7G(5′)ppp(5′)G RNA antireversed cap analog (NEB, S1411S) for the SP6 promoter constructs. The T7 constructs were cotranscriptionally capped with CleanCap Reagent

(5) Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a systemiScienceDecember 15, 2023Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung YuSP6 RNA Polymerase,New England Biolabs,Cat# E2070S Anti-Reverse Cap Analog (ARCA),New England Biolabs,Cat# S1411S EasyTag L-[35S]-Methionine,Perkin Elmer,Cat# NEG709A005UC Lipofectamine 3000,Thermo Fisher Scientific,Cat#

(6) In vitro Analysis of Stalled Ribosomes Using Puromycin IncorporationBio ProtocAugust 20, 2023MaKenzie R. Scarpitti, Michael G. KearseA416P-4)3′-O-Me-m7G(5′)ppp(5′)G RNA Cap Structure Analog (anti-reverse cap analog; ARCA) (New England Biolabs, catalog number: S1411S)Agarose LE, quick dissolve (Apex BioResearch Products, catalog number: 20-102QD)Bromophenol blue (Bio-Rad,

(7) In vitro Analysis of Stalled Ribosomes Using Puromycin IncorporationBio ProtocAugust 20, 2023MaKenzie R. Scarpitti, Michael G. KearseA416P-4)3′-O-Me-m7G(5′)ppp(5′)G RNA Cap Structure Analog (anti-reverse cap analog; ARCA) (New England Biolabs, catalog number: S1411S)Agarose LE, quick dissolve (Apex BioResearch Products, catalog number: 20-102QD)Bromophenol blue (Bio-Rad,

(8) Transgene-free direct conversion of murine fibroblasts into functional muscle stem cellsNPJ Regen MedAugust 8, 2023Xhem Qabrati, Inseon Kim, Adhideb Ghosh, Nicola Bundschuh, Falko Noé, Andrew S. Palmer, Ori Bar-Nurvolume per tube using a nucleotide mix consisting of 6 mM Anti-Reverse Cap Analog (ARCA) (NEB, Cat. #S1411S), 1.5 mM GTP (Megascript T7 kit), 7.5 mM ATP (Megascript T7 kit), 7.5 mM 5-Methylcytidine-5′-Triphosphate

(9) Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer QuantificationVirusesApril 26, 2023Ksenija Korotkaja, Anna Zajakinatranscribed RNAs were capped by adding a 1 mM 3′-O-Me-m7G(5′)ppp(5′)G cap-structure analog (Cat. No. S1411S; New England Biolabs, Ipswich, MA, USA). Then, the DNA template was removed with DNase I (Cat. No. EN0523;

(10) Replication of alphaviruses requires a pseudoknot that involves the poly(A) tailRNAOctober 1, 2022René C.L. OlsthoornPCR template DNA, ATP, CTP, UTP (10 mM each), 2 mM 3′-O-Me-m7G(5′)ppp(5′)G cap structure analog (NEB S1411S), 0.5 mM GTP, 0.5–1 unit of RNase-inhibitor (RNasin, Promega), and 0.5–1 µL of enzyme mix. Incubation

(11) N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein productsCell ReportsAugust 30, 2022Kyusik Q. Kim et al.L-[35S]-Methionine,PerkinElmer,Cat#NEG709A500UC "ATP, [γ-32P]",PerkinElmer,Cat#NEG002A100UC 3′-O-Me-m7G(5’)ppp(5′)G RNA Cap Structure Analog,NEB,Cat#S1411S MEGAscript T7 Transcription Kit,Invitrogen,Cat#AM1334 "T4 RNA Ligase 2, truncated",NEB,Cat#M0242S T4 Polynucleotide Kinase

(12) Differential neutralization and inhibition of SARS-CoV-2 variants by antibodies elicited by COVID-19 mRNA vaccinesNature communicationsJuly 27, 2022Wang L, Kainulainen MH, Jiang N, Di H, Bonenfant G, Mills L, Currier M, Shrivastava-Ranjan P, Calderon BM, Sheth M, et al.concentration of ATP, CTP, and UTP was 7.5 mM, GTP was 3.5 mM, and the Anti-Reverse Cap Analog (NEB, S1411S) was used at 2.8 mM. After 2–3 h of incubation at 30 °C, RNA was cleaned up by PCIA and ethanol precipitated

(13) Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor NetworksCellJanuary 7, 2021Schneider WM, Luna JM, Hoffmann HH, Sánchez-Rivera FJ, Leal AA, Ashbrook AW, Le Pen J, Ricardo-Lax I, Michailidis E, Peace A, et al.HiScribe T7 High Yield RNA Synthesis kit (NEB, cat. #E2040S) and Anti-Reverse Cap Analog (ARCA) (NEB, cat. #S1411S) at a GTP:ARCA ratio of 1:2.8. After in vitro transcription, RNA was treated with Ambion DNase I (ThermoFisher Scientific

(14) mRNA structural elements immediately upstream of the start codon dictate dependence upon eIF4A helicase activityGenome BiologyDecember 30, 2019Joseph A. Waldron, David C. Tack, Laura E. Ritchey, Sarah L. Gillen, Ania Wilczynska, Ernest Turro, Philip C. Bevilacqua, Sarah M. Assmann, Martin Bushell, John Le QuesneK0441) as per the manufacturer’s instructions using 7.5 mM ATP/CTP/UTP, 1.5 mM GTP, and 6 mM ARCA (NEB S1411S), followed by acid-phenol chloroform extraction and ethanol precipitation with ammonium acetate. For

(15) Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock WavesACS applied materials & interfacesMarch 20, 2019Zhang J, Shrivastava S, Cleveland RO, Rabbitts TH.The MEGAscript T7 Transcription Kit (Invitrogen AM1333) was used for mRNA synthesis using ARCA (NEB S1411S) and pseudo-UTP (Jena Bioscience NU-1139S) with a modified protocol. The reaction (1 μg of PCR product,

(16) UGA stop codon readthrough to translate intergenic region of Plautia stali intestine virus does not require RNA structures forming internal ribosomal entry siteRNA (New York, N.Y.)January 1, 2019Kamoshita N, Tominaga SI.packed with Sephadex G-50 (GE Healthcare). Capped RNA was prepared with anti-reverse cap analog (NEB S1411S). In vitro translation Rabbit Reticulocyte Lysate (RRL, Promega L416A) or Transdirect Insect Cell

(17) eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexesNucleic Acids ResearchApril 6, 2018Joseph A Waldron, Farheen Raza, John Le Quesneinstructions. For Supplementary Figure S1, 7.5 mM ATP/CTP/UTP, 1.5 mM GTP and either 6 mM ARCA (NEB S1411S) or 6 mM G(5′)ppp(5′)A RNA Cap Structure Analog (NEB S1406S) was used whereas for Figure 2, 7.5 mM ATP/CTP/UTP,

(18) Design, Assembly, Production, and Transfection of Synthetic Modified mRNAMethods (San Diego, Calif.)January 15, 2018Oh S, Kessler JA.4°C Antarctic Phosphatase,NEB,M0289S,store at −20°C 3′-O-Me-m7G(5′)ppp(5′)G RNA Cap Structure Analog,NEB,S1411S,store at −20°C Pseudouridine-5′-Triphosphate,TriLink BioTechnologies,N-1019,store at −20°C 5-Methylcytidine-5′-Triphosphate,TriLink BioTechnologies

(19) Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γFrontiers in immunologyJanuary 1, 2017Kurena B, Müller E, Christopoulos PF, Johnsen IB, Stankovic B, Øynebråten I, Corthay A, Zajakina A.transcribed rRNAs were capped by adding 1 mM 3′-O-Me-m7G(5′)ppp(5′)G cap-structure analog (Cat. No. S1411S; New England Biolabs, Hitchin, UK) during the transcription reaction. To package the rRNAs into viral

(20) Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegansGeneticsNovember 1, 2013Iskra Katic, Helge Großhansno. E2040S) from plasmid pIK86 (available from Addgene), capped with ARCA (New England Biolabs no. S1411S), LiCl precipitated, and injected at a concentration of 1400 ng/ml together with an sgRNA at a concentration

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