G(5)ppp(5)G RNA Cap Structure Analog from New England Biolabs

Product Specs
ItemG(5)ppp(5)G RNA Cap Structure Analog
CompanyNew England Biolabs
Price $134.00 Inquire
Catalog NumberS1407S
Quantity1 µmol
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafishThe EMBO JournalApril 25, 2025Yizhuang Zhang, Jiasheng Wang, Hailing Fang, Shuqi Hu, Boya Yang, Jiayi Zhou, Raphaëlle Grifone, Panfeng Li, Tong Lu, Zhengyang Wang, et al.purification, the DNA templates were added to the mRNA synthesis mix containing 0.5 mM cap analog (NEB, S1407S), 0.5 mM ATP, 0.5 mM CTP, 0.5 mM UTP, 0.1 mM GTP, 20 U SP6 RNA polymerase (NEB, M0207S), 20 U RNA inhibitor

(2) Lnc-RAINY Regulates Genes Involved in Radiation Susceptibility Through DNA:DNA:RNA triplex-forming Interactions and has Tumor Therapeutic Potential in Lung CancersNon-coding RNA ResearchDecember 18, 2024Emily S. Westemeier-Rice, Michael T. Winters, Travis W. Rawson, Kiran J. Patel, Olivia McHugh, Sierra Ward, Sarah McLaughlin, Amanda Stewart, Bishal Misra, Sebastian Dziadowicz, et al.manufacturer's instructions. The RNAs were capped via G(5′)ppp(5″)G RNA cap structure analog Kit (NEB kit #S1407S) according to manufacturer's instructions. RNA was cleaned using the Monarch RNA Cleanup Kit (NEB kit

(3) Despite the odds: formation of the SARS-CoV-2 methylation complexNucleic Acids ResearchJune 24, 2024Alex Matsuda, Jacek Plewka, Michał Rawski, André Mourão, Weronika Zajko, Till Siebenmorgen, Leanid Kresik, Kinga Lis, Alisha N Jones, Magdalena Pachota, et al.SAM (from the Cisbio's kit), 187.5 μM RNA cap analog (GpppA or GpppG, New England Biolabs S1406S and S1407S, respectively) or 18.75 μM Cap 0 RNA oligo (TriLink, produced on request) in reaction buffer consisting

(4) The eukaryotic translation initiation factor eIF4E reprograms alternative splicingThe EMBO journalApril 3, 2023Ghram M, Morris G, Culjkovic-Kraljacic B, Mars JC, Gendron P, Skrabanek L, Revuelta MV, Cerchietti L, Guzman ML, Borden KLB.Dynabeads G per mg of extract at 4°C for 40 min and then treated with 100 or 200 micromolar or GpppG (NEB S1407S) or m7GpppG (NEBS 1404S) with rotation. For 1 mg IP, 33 μl of Dynabeads G (Invitrogen) were preincubated

(5) A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19SLAS DiscoveryJune 1, 2021Sumera Perveen, Aliakbar Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadirespectively; G(5′)ppp(5′)A RNA (GpppA) (NEB, cat. S1406S); and G(5′)ppp(5′)G RNA (GpppG) (NEB, cat. S1407S) are from New England Biolabs (NEB, Whitby, Ontario, Canada). NEB RNA reagents were gifts from Dr. Peter

(6) METTL3/METTL14 Transactivation and m⁶A-Dependent TGF-β1 Translation in Activated Kupffer CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Feng Y, Dong H, Sun B, Hu Y, Yang Y, Jia Y, Jia L, Zhong X, Zhao R.full-length firefly luciferase were used as templates. Transcripts with noncap, nonfunctional (ApppG) cap (S1407S; New England Biolabs), and normal (m7G) cap (S1404S; New England Biolabs) were generated using the T7

(7) CRISPR-Cas9-Mediated Genomic Deletions Protocol in ZebrafishSTAR ProtocolsDecember 30, 2020João Pedro Amorim, Renata Bordeira-Carriço, Ana Gali-Macedo, Chiara Perrod, José BessaSP6 RNA Polymerase (Thermo Fisher Scientific #EP0131) for Cas9.Note: Only for Cas9: 5′ Cap (New England Biolabs #S1407S)a.Mix the following and incubate at 37°C:i.15 μL H2Oii.10 μL Transcription buffer 5×iii.5 μL dithiothreitol (DTT

(8) The GCN2-ATF4 Signaling Pathway Induces 4E-BP to Bias Translation and Boost Antimicrobial Peptide Synthesis in Response to Bacterial InfectionCell reportsNovember 21, 2017Vasudevan D, Clark NK, Sam J, Cotham VC, Ueberheide B, Marr MT 2nd, Ryoo HD.described above. For experiments with excess m7G cap, a cap structure analogue (New England Biolabs, #S1407S) was added to a final concentration of 1 mM. Supplementary Material

(9) Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegansGeneticsNovember 1, 2013Iskra Katic, Helge GroßhanspDR274 (Hwang et al. 2013b) were similarly transcribed in vitro and capped (New England Biolabs no. S1407S), column puriﬁed (Ambion no. AM10070), and boiled for 1 min before assembling the injection mix. A

(10) The insulin receptor cellular IRES confers resistance to eIF4A inhibitioneLifeJuly 16, 2013Calla M Olson, Marissa R Donovan, Michael J Spellberg, Michael T Marrsubstrate (Promega). For assays containing excess m7G cap, cap structure analogue (New England Biolabs, #S1407S) was added to a final concentration of 1 mM. All experiments were performed at least twice in triplicate. Cell culture

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