G(5)ppp(5)A RNA Cap Structure Analog from New England Biolabs

Product Specs
ItemG(5)ppp(5)A RNA Cap Structure Analog
CompanyNew England Biolabs
Price $155.00 Inquire
Catalog NumberS1406S
Quantity1 µmol
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolutionNat CommunJuly 20, 2025Yuanbo Liu, Dean Li, Xueting Wang, Kongyan Niu, Jun Bai, Lefeng Qu, Nan LiuFAD-RNA), 4 mM dpCoA (Sigma-Aldrich, catalog: D3385, for dpCoA-RNA) or 4 mM m7GpppA (NEB, catalog: S1406S, for m7GpppA-RNA). Additionally, 10 μL of T7 RNA polymerase (Thermo Fisher Scientific, catalog: AM1334),

(2) Despite the odds: formation of the SARS-CoV-2 methylation complexNucleic Acids ResearchJune 24, 2024Alex Matsuda, Jacek Plewka, Michał Rawski, André Mourão, Weronika Zajko, Till Siebenmorgen, Leanid Kresik, Kinga Lis, Alisha N Jones, Magdalena Pachota, et al.Ultrapure SAM (from the Cisbio's kit), 187.5 μM RNA cap analog (GpppA or GpppG, New England Biolabs S1406S and S1407S, respectively) or 18.75 μM Cap 0 RNA oligo (TriLink, produced on request) in reaction buffer

(3) CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral ReplicationVirusesMay 3, 2024Shivaprasad S, Qiao W, Weng KF, Umashankar P, Carette JE, Sarnow P.and rUTP, 10 µL of 10 X reaction buffer, 10 µL of T7 enzyme mix, and 12.5 µL of 5′GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 µL for 30 min at 30 °C. 2.6 µL of 75 mM rATP was added to the

(4) Hidden features of NAD-RNA epitranscriptome in Drosophila life cycleiScienceJanuary 19, 2024Shuwen Ge, Xueting Wang, Yingqin Wang, Minghui Dong, Dean Li, Kongyan Niu, Tongyao Wang, Rui Liu, Chao Zhao, Nan Liu, et al.with 1mM ATP (for pppA-RNA), 4 mM NAD (for NAD-RNA) or 4 mM m7GpppA (New England Biolabs, catalog: S1406S) (for m7G-RNA), 10 μL of T7 RNA polymerase (Thermo Fisher Scientific, catalog: AM1334), 5% DMSO, 5 mM

(5) Epitranscriptome analysis of NAD-capped RNA by spike-in-based normalization and prediction of chronological ageiScienceDecember 15, 2023Dean Li, Shuwen Ge, Yandong Liu, Miaomiao Pan, Xueting Wang, Guojing Han, Sili Zou, Rui Liu, Kongyan Niu, Chao Zhao, et al.each of GTP, CTP and UTP, with 4 mM NAD (for NAD-RNA) or 4 mM m7GpppA (New England Biolabs, catalog: S1406S) (for m7G-RNA), 10 μL of T7 RNA polymerase (Promega, catalog: P1300), 5% DMSO, 5 mM DTT and 2.5-unit

(6) La-related protein 4 is enriched in vaccinia virus factories and is required for efficient viral replication in primary human fibroblastsMicrobiology SpectrumOctober 17, 2023Pragyesh Dhungel, Djamal Brahim Belhaouari, Zhilong Yangused in this study was capped with either m7G (Anti-Reverse Cap Analog, S1411L) or ApppG cap analog (S1406S, New England Biolabs). Knockdown using small interfering RNA The small interfering RNAs (siRNAs) used

(7) Structures of SARS-CoV-2 N7-methyltransferase with DOT1L and PRMT7 inhibitors provide a platform for new antiviralsPLOS PathogensJuly 31, 2023Jithesh Kottur, Kris M. White, M. Luis Rodriguez, Olga Rechkoblit, Richard Quintana-Feliciano, Ahana Nayar, Adolfo García-Sastre, Aneel K. Aggarwalwere performed with a Microcal ITC200 instrument at 25°C. SAM/SGC0946/SGC8158/GpppA cap analog (NEB # S1406S) were loaded in the syringe (500μM) and titrated into 50μM of nsp14/nsp10 complex in the cell. The titrations

(8) La-related protein 4 is enriched in vaccinia virus factories and is required for efficient viral replication in primary human fibroblastsbioRxivMarch 15, 2023Pragyesh Dhungel, Djamal Brahim Brahim Belhaouari, Zhilong Yangthis study was capped with either m7G (Anti-Reverse Cap Analog [ARCA], S1411L) or ApppG cap analog (S1406S, New England Biolabs). Knockdown using small interfering RNA (siRNA) The small interfering RNAs (siRNAs)

(9) ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNANucleic Acids ResearchJanuary 25, 2023Kongyan Niu, Jinyang Zhang, Shuwen Ge, Dean Li, Kunfeng Sun, Yingnan You, Jiaqian Qiu, Kun Wang, Xueting Wang, Rui Liu, et al.with 1mM ATP (for normal RNA), 4 mM NAD (for NAD-RNA) or 4 mM m7GpppA (New England Biolabs, catalog: S1406S) (for m7G-RNA), 10 μl of T7 RNA polymerase (Promega, catalog: P1300), 5% DMSO, 5 mM DTT and 2.5-unit

(10) Multi-target mechanisms against coronaviruses of constituents from Chinese Dagang Tea revealed by experimental and docking studiesJournal of EthnopharmacologyOctober 28, 2022Liyun Zhao, Xubing Qin, Tingting Lin, Fuda Xie, Liyuan Yao, Yulin Li, Binhong Xiong, Zhifang Xu, Yongchang Ye, Hongfeng Chen, et al.previously dispensed inhibitors (0.4 μL), Reaction was started with 10 μl of a mix containing both GpppA (S1406S, NEB) and SAM at 8 μM and 2 μM final concentrations respectively and was incubated 20 min at 30 °C.

(11) High-resolution structures of the SARS-CoV-2 N7-methyltransferase inform therapeutic developmentNature Structural & Molecular BiologySeptember 8, 2022Jithesh Kottur, Olga Rechkoblit, Richard Quintana-Feliciano, Daniela Sciaky, Aneel K. Aggarwalprotein (nsp14/10, MTase domain or TEL-MTase), 20 μM SAM and 0.15 mM G(5′)ppp(5′)A RNA cap analog (NEB, S1406S) were incubated for 1 h at room temperature. The detection solution from the kit was then added, and

(12) Loquacious modulates flaviviral RNA replication in mosquito cellsPLoS pathogensApril 1, 2022Shivaprasad S, Weng KF, Ooi YS, Belk J, Carette JE, Flynn R, Sarnow P.rGTP and rUTP, 10 μl of 10X reaction buffer, 10 μl of T7 enzyme mix and 12.5 μl of 5’GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 μl for 30 min at 30°C. 2.6 μl of 75mM rATP was added to the

(13) The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNAPLoS pathogensSeptember 1, 2021Vera-Otarola J, Castillo-Vargas E, Angulo J, Barriga FM, Batlle E, Lopez-Lastra M.NTP/ACAP mix. The 2X T7 NTP/ACAP mix contained the A(5’)ppp(5’)G cap-analog (6 mM final concentration; #S1406S; New England Biolabs, Ipswich, MA, USA) and NTPs (7.5 mM final concentration of CTP, ATP, UTP and 1.5

(14) A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19SLAS DiscoveryJune 1, 2021Sumera Perveen, Aliakbar Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadito as N7-meGpppG and N7-meGpppA for consistency, respectively; G(5′)ppp(5′)A RNA (GpppA) (NEB, cat. S1406S); and G(5′)ppp(5′)G RNA (GpppG) (NEB, cat. S1407S) are from New England Biolabs (NEB, Whitby, Ontario,

(15) Rocaglates Induce Gain-of-Function Alterations to eIF4A and eIF4FCell ReportsFebruary 25, 2020Jennifer Chu et al.m7G(5′)ppp(5′)G RNA cap structure analog,New England Biolabs,Cat#: S1404S G(5′)ppp(5′)A RNA Cap Structure Analog,New England Biolabs,Cat#: S1406S AMP-PNP,Sigma-Aldrich,Cat#: 10102547001 Biotin-11-ATP,Perkin Elmer,Cat#: NEL544001EA Biotin-11-UTP,Perkin Elmer

(16) Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper proteinNucleic Acids ResearchNovember 16, 2018C Joaquín Cáceres et al.with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs). In vitro translation In vitro translation reactions were carried out in nuclease-treated

(17) eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexesNucleic Acids ResearchApril 6, 2018Joseph A Waldron, Farheen Raza, John Le QuesneATP/CTP/UTP, 1.5 mM GTP and either 6 mM ARCA (NEB S1411S) or 6 mM G(5′)ppp(5′)A RNA Cap Structure Analog (NEB S1406S) was used whereas for Figure 2, 7.5 mM ATP/CTP/UTP, 6 mM ARCA (S1411S) and either 1.5 mM GTP or 1.5

(18) Retention of exogenous mRNAs selectively in the germ cells of the sea urchin requires only a 5′-cap and a 3′-UTRMolecular Reproduction and DevelopmentJuly 1, 2013Nathalie Oulhen, Gary M. Wessel3′-O-Me-m7G(5′)ppp(5′)G (New England Biolabs, Ipswich, MA; S1411S) or the G(5′)ppp(5′)A (New Englands Bioloabs-S1406S) cap analog during the RNA synthesis. Injection solutions contain: 20% glycerol, RNA at 100 ng/μl, and

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