SurePrep RNA/Protein Purification Kit from Fisher BioReagents

• Fast and easy processing using rapid spin-column format
  
• All columns for RNA purifi cation and protein purification
  provided
  
• Sequentially isolate nucleic acids and proteins from a
  single lysate: no need to split the lysate
  
• Isolate all sizes of RNA, from large mRNA and rRNA
  down to microRNA (miRNA)
  
• No phenol or chloroform extractions
  
• Isolate high quality total RNA
  
• High yields of isolated proteins

KIT SPECIFICATIONS:

Column Binding Capacity ......................................50μ g RNA

200μ g for protein

Maximum Column Loading Volume ................600μ L

Size of RNA Purified ................................................All sizes, including small RNA (<200 nt)

Maximum Amount of Starting Material

Animal Cells ................................................................ 3 x 10⁶ cells

Animal Tissues ..........................................................25mg

Blood .................................................................................100μ L

Bacteria .......................................................................... 1 x 10⁹ cells

Yeast .................................................................................. 1 x 10⁸ cells

Fungi .................................................................................50mg

Plant Tissues ...............................................................50mg

Time to complete purification process ........30 minutes for 10 purifications

Average Yields from RNA Purification

HeLa Cells (1 x 10⁶ cells) ......................................15μ g RNA

HeLa Cells (1 x 10⁶ cells) ......................................150μ g protein