Fig 1: shRNA-mediated decrease in LGR5 expression results in inhibition of cell migrationTPC-1 clonal cells harboring stable expression for either non-targeting (Ctrl) shRNA A. or targeting human LGR5 shRNA B. were subjected to a wound healing assay for 24 and 48 h. Representative images are shown. Leading edges of repairing cell monolayers are indicated by red bars. Briefly, cells were grown to 80% confluence at which time cells were removed with a P200 tip. Images were then acquired at 0 h, 24 h, and 48 h and analyzed by Olympus software to measure ability of the cells to repair the wound. C. Cellular migration was quantified and represented graphically with results expressed as mean ± SEM of three independent measurements. Significance p < 0.05*.