Description
Self-amplifying RNA (saRNA) can use its own RNA sequence as a template for self-replication, thereby achieving the same level of protein expression as traditional mRNA at lower doses. This extends the presence of the antigen protein in the body, further enhancing the vaccine's immune efficacy. eGFP saRNA is a self-amplifying RNA synthesized in vitro. It features a replicase expression sequence structure derived from the Venezuelan equine encephalitis virus (VEEV), enabling self-replication both in vivo and in vitro. This allows for low-dose, high-level, and long-lasting expression of the target gene. eGFP is an enhanced green fluorescent protein with maximum excitation/emission wavelengths of 488 nm and 509 nm, respectively. When transfected into cells, it can express strong green fluorescence, making it suitable as a control in studies of target gene transfection and expression in mammalian cells. This product is a saRNA synthesized in vitro using the T7 RNA Polymerase 2.0 (Novoprotein, Cat.No.: E122). It features a 5' Cap 1 structure and a 3' poly(A) tail, with a fragment length of 8789 nucleotides (nt)