Prime-a-Gene Labeling System from Promega

Product Specs
ItemPrime-a-Gene Labeling System
CompanyPromega
Price $167.00Supplier Page View Company Product Page
Catalog NumberU1100
Quantity30 reactions
ApplicationsLabeling

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) SUMO-Chain-Regulated Proteasomal Degradation Timing Exemplified in DNA Replication InitiationMolecular cellNovember 21, 2019Psakhye I, Castellucci F, Branzei D.28106 ProbeQuant G-50 micro columns,GE Healthcare,Cat# 28903408 Prime-a-Gene labeling system,Promega,Cat# U1100 "Invitrogen Bolt 4-12% Bis-Tris Plus Gels, 15-well",Thermo Fisher Scientific,Cat# NW04125BOX "L-Arginine:HCl

(22) The m⁶A pathway protects the transcriptome integrity by restricting RNA chimera formation in plantsLife science allianceJune 1, 2019Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, Llauro C, Azevedo J, Laudié M, Attina A, et al.GeneClean Turbo Kit (Cat. No. 111102400; MP Biomedicals), and labeled with the Prime-a-Gene Labeling System (Cat. No. U1100; Promega) according to the manufacturer's instructions. The hybridization was performed in the PerfectHyb

(23) Simplified Transformation of Ostreococcus tauri Using Polyethylene GlycolGenes (Basel)May 26, 2019Frédéric Sanchez, Solène Geffroy, Manon Norest, Sheree Yau, Hervé Moreau, Nigel Grimsley(Perkin-Elmer ref BLU008H250UC) according to the manufacturer’s instructions (Prime-a-Gene kit, Promega ref U1100) for use as a DNA probe. Dried gels were equilibrated in hybridisation buffer (6 × SSC, 5 × Denhardt’s

(24) Toxicity of overexpressed MeCP2 is independent of HDAC3 activityGenes & DevelopmentDecember 1, 2018Martha V. Koerner et al.chromosome 11: 104,142,739–104,143,549) were labeled using the Prime-a-Gene labeling system (Promega, U1100) with α32-dCTP. Blots were washed twice for 30 min in Church wash (1% SDS, 20 mM Na2HPO4) and exposed

(25) RNase E cleavage shapes the transcriptome ofRhodobacter sphaeroidesand strongly impacts phototrophic growthLife Science AllianceAugust 1, 2018Konrad U Förstner, Carina M Reuscher, Kerstin Haberzettl, Lennart Weber, Gabriele Klugwas labeled with α-[32P]-dCTP (SRP-205; Hartmann Analytic) using the Prime-a-Gene Labeling System (U1100; Promega) as described in the manufacturer’s manual. Source data for the Northern blots are provided

(26) ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlingsJournal of Experimental BotanyMay 25, 2018Tian Jiang, Jing Zhang, Liwei Rong, Yanjiang Feng, Qi Wang, Qiulai Song, Lixin Zhang, Min Ouyangwere prepared by PCR amplification and labeled using the Prime-a-Gene Labeling System (SGMB01-Promega-U1100). RNA was used to generate first-strand cDNA in a 20-μl reaction using the Superscript III cDNA synthesis

(27) A novel brown adipocyte-enriched long non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytesBiochimica et biophysica acta. Molecular and cell biology of lipidsApril 1, 2018Xiong Y, Yue F, Jia Z, Gao Y, Jin W, Hu K, Zhang Y, Zhu D, Yang G, Kuang S.labeled with α-32p-cytidine triphosphate (CTP) using DNA polymerase 1 Large (Klenow) Fragment (Promega, U1100). RNA blots were hybridized in ULTRAhyb (Ambion) at 68 °C overnight, washed twice (5min) with 2 × saline

(28) FRG3, a Target of slmiR482e-3p, Provides Resistance against the Fungal Pathogen Fusarium oxysporum in TomatoFrontiers in Plant ScienceJanuary 25, 2018Hui-Min Ji, Min Zhao, Ying Gao, Xin-Xin Cao, Hui-Ying Mao, Yi Zhou, Wen-Yu Fan, Katherine A. Borkovich, Shou-Qiang Ouyang, Peng Liu1). Probes were labeled using the random priming method according to the manufacturer’s protocol (#U1100, Promega, San Luis Obispo, CA, United States). All blots were stripped and reprobed using 18S RNA probe

(29) Charting organellar importomes by quantitative mass spectrometryNature communicationsMay 9, 2017Peikert CD, Mani J, Morgenstern M, Käser S, Knapp B, Wenger C, Harsman A, Oeljeklaus S, Schneider A, Warscheid B.gel-purified and radioactively labelled using the Prime-a-Gene labelling system (Promega, Product No. U1100). A list of oligonucleotide primers is provided (Supplementary Data 13). Full scans Northern blots are

(30) Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoDNature CommunicationsApril 1, 2017Xiaohua Yu et al.labelled with α-32p-cytidine triphosphate (CTP) using DNA polymerase 1 Large (Klenow) Fragment (Promega, U1100). RNA blots were hybridized in ULTRAhyb (Ambion) at 68 °C overnight, washed twice (5 min) with 2 × saline

(31) Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotesPLOS ONEMarch 16, 2017R. J. Bevacqua et al.Tn5 transposon was labeled by nick translation with α-32P CTP (Prime-a-Gene Labeling System Promega, U1100). As positive controls, 10 pg and 1 ng SalI-XbaI pCX-EGFP 1.6 Kb fragment were included. Animal Southern blot

(32) cis -Encoded Small RNAs, a Conserved Mechanism for Repression of Polysaccharide Utilization in BacteroidesJournal of BacteriologySeptember 15, 2016Yanlu Cao, Konrad U. Förstner, Jörg Vogel, C. Jeffrey Smithtwo probes, donC and donG, that were labeled by PCR using a Prime-a-Gene labeling system (Promega catalog no. U1100) and primers described in Table S1 in the supplemental material. RNA samples (30 ␮g of total RNA) were

(33) Production and characterization of recombinant human acid a-glucosidase in transgenic rice cell suspension cultureJournal of BiotechnologyMarch 21, 2016Jae-Wan Jung, Nan-Sun Kim, Seon-hui Jang, Yun-Ji Shin, Moon-Sik Yang

(34) Expression of the Rice Arginase Gene OsARG in Cotton Influences the Morphology and Nitrogen Transition of SeedlingsPLOS ONENovember 3, 2015Zhigang Meng, Zhaohong Meng, Rui Zhang, Chengzhen Liang, Jianmin Wan, Yanling Wang, Honghong Zhai, Sandui Guomembrane. The probes were labeled using a Prime-a-Gene® Labeling System (Promega Co., USA; Cat. No.U1100) according to the manufacturer’s instructions. The templates for [α-32P] dCTP probe labeling were products

(35) Characterization of Fusarium Strains Recovered From Wheat With Symptoms of Head Blight in KentuckyPlant DiseaseNovember 1, 2015S. Bec, T. Ward, M. Farman, K. O’Donnell, D. Hershman, D. Van Sanford, L. J. VaillancourtThe purified product (50 to 80 ng) was labeled using the Prime-a-gene labeling system (Promega, cat. #U1100) and used for Southern hybridization analysis. Repetitive fingerprinting probes. Repetitive motifs present

(36) Salt-induced transcription factorMYB74is regulated by the RNA-directed DNA methylation pathway inArabidopsisJournal of Experimental BotanySeptember 1, 2015Rui Xu, Yuhan Wang, Hao Zheng, Wei Lu, Changai Wu, Jinguang Huang, Kang Yan, Guodong Yang, Chengchao Zhengamplified by primers (listed in Supplementary Table S1) with the Prime-a-Gene Labeling System (Promega U1100) and 32P. The filters were washed twice at 37 °C in buffer containing 2×SSC (0.3M NaCl and 0.03M sodium

(37) Evolution of Mating Systems in Basidiomycetes and the Genetic Architecture Underlying Mating-Type Determination in the Yeast Leucosporidium scottiiGeneticsSeptember 1, 2015Teresa M Maia, Susana T Lopes, João M G C F Almeida, Luiz H Rosa, José Paulo Sampaio, Paula Gonçalves, Marco A Coelhoindicated in Table S3 and labeled with [a-32P] dATP using the Prime-a-Gene Labeling system (Promega, #U1100). Standard protocols were used for hybridization and washing steps. Radioactive signals were detected

(38) MicroRNAs Suppress NB Domain Genes in Tomato That Confer Resistance to Fusarium oxysporumPLoS PathogensOctober 16, 2014Shouqiang Ouyang, Gyungsoon Park, Hagop S. Atamian, Cliff S. Han, Jason E. Stajich, Isgouhi Kaloshian, Katherine A. BorkovichS5). Probes were labeled using the random priming method according to the manufacturer's protocol (#U1100, Promega, San Luis Obispo, CA). Blots were stripped and reprobed using an 18S rRNA probe as a loading

(39) Production of functional recombinant bovine trypsin in transgenic rice cell suspension culturesProtein Expression and PurificationSeptember 20, 2010Nan-Sun Kim, Hwa-Young Yu, Nguyen-Duc Chung, Yun-Ji Shin, Tae-Ho Kwon, Moon-Sik Yang

(40) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plantsProtein Expression and PurificationMay 6, 2010Tae-Geum Kim, Mi-Young Kim, Moon-Sik Yang

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