GoTaq qPCR Master Mix from Promega

Product Specs
ItemGoTaq qPCR Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6002
Quantity25ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 ActivatorInternational Journal of Stem CellsFebruary 28, 2028Changyeong Kim, Kyung Hwan Park, Soo-Been Jeon, A-Reum Han, Ji Yoon Lee, Young-sup Yoonquantitative (q) RT-PCR using specific primers (Supplementary Table S1), GoTaqⓇ qPCR Master Mix (Promega, A6001), and the QuantStudioTM 3 System (Thermo Fisher Scientific). The relative mRNA expression of target

(2) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMedical Gas ResearchJune 1, 2026Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing ZhaoCo., Ltd., Osaka, Japan, RR047A)9 and GoTaq qPCR master mix (Promega Corporation, Madison, WI, USA, A6001).10 The CFX96 touch real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA; C1000 Touch

(3) In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signalsDevelopmental BiologyMay 1, 2026Pamela Kairath, Pei Xuan Er, Sean Wilson, Irene Ghobrial, Jessica M. Vanslambrouck, Yi-Hsien Yi-Hsien, Pruett-Miller Shondra Pruett-Miller, Sanjay Jain, Melissa H. Littlefollowing manufacturer’s instructions. Quantitative PCR were performed using GoTaq qPCR master mix (Cat#A6001, Promega). The reaction was conducted on an ABI Real-time PCR (Applied Biosystems) machine. Amplification

(4) Pulsed Electric Fields as an Effective Tool for Toxoplasma gondii InactivationFoodsApril 21, 2026Vanesa Abad, Daniel Berdejo, Juan Manuel Martínez, Nabil Halaihel, João Luis Garcia, Ignacio Álvarez-Lanzarote, Susana Bayarri, Guillermo Cebriánreal-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA) with GoTaq SYBR Green Master Mix (catalogue # A6002, Promega) and the primers listed in Table 1. The total reaction volume was 22 µL, consisting of 10 µL

(5) A Novel Mechanism for Depot-Specific Leptin Gene Expression Regulation and its Persistence After Weight LossiScienceApril 17, 2026Natalie Taege, Jan Hendric Britsemmer, Andreas Israel, Christin Krause, Sina Junge, Annette Feuchtinger, Siegfried Ussar, Paul Thomas Pfluger, Timo Gemoll, Sonja Charlotte Schriever, et al.Germany",D4074 RevertAid cDNA synthesis kit,"ThermoFisherScientific, US",K1622 GoTAQ qPCR System,"Promega, US",A6001 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, 3T3-L1,"American

(6) Transient remodeling of gut metabolism supports juvenile growth and adult fitness in DrosophilaNat CommunApril 13, 2026Clara Lefranc, Arnaud Fichant, Gilles Storellithe manufacturer’s protocol. cDNA were then diluted 1/10 (v/v) in nuclease-free water (Promega, Cat#A6001). RT-qPCR reactions were performed on cDNA using GoTaq qPCR Master Mix (Promega, Cat#A6001), on a QuantStudio

(7) Tumor-suppressive role of miR-216b impacts cell cycle regulation in high-grade gliomaActa Neuropathol CommunApril 6, 2026Alexandra Lang, Sabine Buerger, Leah Mager, Lea Erjavc, Barbara Kiesel, Sibylle Madlener, Carola Nadine Jaunecker, Linda Pacher, Finn Christopher Robert, Leonie Krausgruber, et al.CDK4 and CDK6 gene expression the GoTaq® qPCR Kit from Promega (Madison, Wisconsin, USA) was used (A6001) and GAPDH was used as housekeeping gene. Detailed information can be found in the Supplementary Table

(8) Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cellsNat MetabApril 3, 2026Mu A, Gang Wang, Nathan W. Zammit, Laura Roth, Yasheng Maierhaba, Dina Bogoslavski, Chufan Cai, P. Kent Langston, Qiuyang Zhang, Inna S. Afonina, et al.Biosystems). Briefly, cDNA was mixed with 250–500 nmol primers and the GoTaq qPCR System (Promega, A6002). Relative mRNA levels of the gene of interest were normalized to the mRNA level of Rplp0, and fold

(9) Mini‐Catalytically Inactive Cas13X‐Derived RNA Base Editing of β‐Catenin Attenuates Pulmonary Damage in a Murine Acute Lung Injury ModelMedComm (2020)April 1, 2026Wenyi Liu, Wanda Bi, Saiying Hou, Juan Du, Ling Zeng, Anqiang Zhang, Huacai Zhang, Dalin Wen, Qingli Cai, Chu Gao, et al.with the cDNA for each sample on a Bio‐Rad CFX real‐time system using SYBR qPCR Master Mix (Promega, A6002). Relative expression was calculated by 2(−ΔΔCt) with GAPDH as reference. And target genes were normalized

(10) Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling AxisInternational Journal of Molecular SciencesMarch 31, 2026Grace L. Wong, Munazza S. Khan, Sara Manore, Shivani Bindal, Ravi Singh, Hui-Wen Lo#18080093; Invitrogen, Carlsbad, CA, USA), and qPCR was performed using GoTaq qPCR Master Mix (cat. #A6001; Promega, Madison, WI, USA). Relative quantification was performed using the 2−ΔΔCT method. qPCR primers

(11) MIF-Induced CD74+ Microglia and Macrophages Promote Progression of Brain Metastasis and are Clinically Relevant Across Central Nervous System DisordersCancer ResMarch 24, 2026Laura Alvaro-Espinosa, Angel Marquez-Galera, Neibla Priego, Virginia García-Calvo, Maria Perea-García, Carolina Hernandez-Oliver, Diana Retana, Oliva Sanchez, Ana de Pablos-Aragoneses, Pedro García-Gómez, et al.Bio-Rad). Gene expression was analyzed using SYBR green gene expression assays (GoTaq qPCR Master Mix, A6002, Promega). The relative gene expression was normalized to a ‘housekeeping’ gene. The following primers

(12) Dopamine enhances surface glial glycolysis via acetylcholine and insulin/insulin-like growth factor signaling in the honeybeeiScienceMarch 20, 2026Miaoran Zhang, Xingan Li, Yunxiao Zhang, Huanyu Zhang, Meng Xu, Jieluan Li, Qingsheng Niu, Wei Feng, Aowen Tian, Peng Chen30 min, and 85°C for 5 min. qPCR reactions were performed with the GoTaq qPCR reagent kit (Promega, A6001). Primer sequences are listed in Table S2. Reaction mixtures were prepared according to the manufacturer’s

(13) Regulation of YAP activity by nuclear G-actin bindingNucleic Acids ResMarch 19, 2026Hong Wang, Irma M Jayawardana, Justus M Fleisch, Dennis Frank, Bing Zhao, Christos Kamaras, Peter Gebhardt, Julian Knerr, Svenja Ulferts, Shamphavi Sivabalasarma, et al.µl cDNA or recovered DNA from CHIP, 200 nM primers, and 10 µl GoTaq® qPCR Master Mix (2X) (Promega, A6002). For relative gene expression analysis, the expression levels of target genes were normalized to those

(14) A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3Noncoding RNA ResMarch 19, 2026Xiaoyang Cao, Juzheng Sui, Ge Shan, Xu Liustandard protocol. Quantitative PCR was performed using GoTaq SYBR Green qPCR Master Mix (Promega, A6001). Reactions were run on a QuantStudio 3 Real-Time PCR system (Thermo Scientific) following standard

(15) Neurotrophic Modulation Restores Motor and Developmental Defects in Zebrafish Models of ints11 DeficiencyJ NeurochemMarch 16, 2026Anna Pistocchi, Elena Chiricozzi, Matilde Molteni, Gaia Galassi, Laura Mauri, Francesca Balistreri, Stefania Magri, Anna Marozzi, Franco Taroni, Alex PezzottaA5000). qPCR reactions were carried out with iQ SYBR Green Supermix (Promega; Madison, WI, USA; cat. no A6002) on a QuantStudio 5 Real‐Time PCR System (Applied Biosystems, Waltham, MA, USA). Gene expression levels

(16) Imperatorin Mitigates Rosacea-Like Inflammation by Modulating the Crosstalk Between JNK1 and STAT1FASEB JMarch 15, 2026Xiaoxue Wang, Zexin Zhumanufacturer's instructions. Gene expression was quantified using the Promega GoTaq qPCR Master Mix (Promega, A6001) on a quantitative PCR (qPCR) machine. The relative mRNA levels were normalized to GAPDH and analyzed

(17) A druggable redox switch on SHP1 controls macrophage inflammationNature Chemical BiologyMarch 12, 2026Mei Ying Ng, Meredith N. Nix, Guangyan Du, Ivan Davidek, Nils Burger, Sanghee Shin, Sean Toenjes, Haruna Takeda, Megan Cheah Xin Cheah Xin Yan, Bingsen Zhang, et al.QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) using GoTaq qPCR Master Mix (Promega, #A6001). Fold changes in expression were calculated by the Delta Delta Ct method using human glyceraldehyde-3-phosphate

(18) Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9Front Cell Dev BiolMarch 11, 2026Fulin Zhou, Siyu Yang, Qiang Lin, Ruling Zhang, Yingshi Su, Yibo Hou, Jin Wang, Jiaye Mo, Yubo Zhang, Xiaoyong Dai, et al.levels of CA9 and GAPDH (used as an internal control) were detected using qPCR Master Mix (Promega, A6002) through qRT-PCR instrument (Analytikjena, qTOWER3G). The specified genes were amplified using the primer

(19) Modulatory Effects of Cirsimarin on Lung Cancer A549 Cells Migration in 2D and 3D Cultures Involves Transcriptional Regulation of MetalloproteinasesJ Biochem Mol ToxicolMarch 9, 2026Anna Gabriele Prado Dos dos Santos, Celina Yung-Ai Lin Lee, Érica Romão Pereira, Andresa Hiromi Sakai, Diego Luís Ribeiro, Victor Antônio Silva Lima, Marcos Bispo Pinheiro Camara, Cláudia Quintino da da Rocha, Sabine A. S. Langie, Ilce Mara de Syllos Cólus, et al.using 500 ng of RNA, oligo‐DT, and random primers and RT‐qPCR using GoTaq qPCR Master Mix (Cat. N° A6001; Promega, USA) and StepOnePlus System thermocycler (Applied Biosystems). The target mRNAs for modulation

(20) Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitroFront PharmacolMarch 9, 2026Ziyan Xu, Hao Wu, Weiyang Fan, Fenzhao Meng, Shuangfei Hu, Muhan Zou, Yixuan Chen, Weiwei Su, Peibo LicDNA (B532451-0020, Sangon Biotech, China). RT-qPCR was then performed using GoTaq qPCR Master Mix (A6002, Promega, United States), following the manufacturer’s instruction. Reaction procedures were 95 °C for

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