Immun-Blot® PVDF Membranes from Bio-Rad

Product Specs
ItemImmun-Blot® PVDF Membranes
CompanyBio-Rad
Price Supplier Page
Catalog Number1620177 / 1620174 / 1620175 / 1620176 / 1620255 / 1620238 / 1620239 / 1620218 / 1620219

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Sirtuin 5-mediated desuccinylation of PRDX6 inhibits ferroptosis and alleviates sepsis-associated acute kidney injuryRedox RepDecember 31, 2026Wenlong Lin, Caitao Dong, Qinhong Jiang, Yuanquan Lou, Long Wang, Ziqi He(Solarbio, Beijing, China). The proteins were separated by SDS-PAGE, then moved to PVDF membranes (1620177, BIO-RAD, USA), blocked for 15 minutes with rapid blocking buffer (PS108P, Epizyme, China), washed four

(2) miR-17-5p inhibits classical swine fever virus replication by targeting the PKD2 -regulated AMPK/mTOR autophagy pathwayVirulenceDecember 31, 2026Xinxian Wang, Shurui Yang, Lichun Xie, Shanshan Qi, Libo Gao, Yongmei Li, Qian Li, Junlong Bi, Jianping Liu, Yongneng Li, et al.electrophoresis, proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad; Cat. #1620177) at a constant current of 180 mA for 1 h. Membranes were blocked with 5% skim milk in TBST for 1 h at

(3) Unbiased CRISPR synthetic lethal screening for genetic vulnerabilities in a succinate dehydrogenase-loss model of paragangliomaiScienceJune 19, 2026Fatimah J. Al Khazal, Michael J. Emch, Cristina Correia, Judith Favier, John R. Hawse, L. James MaherBCA protein assay kit,Pierce,A55864 NuPAGE 10% Bis-Tris protein gels,Invitrogen,NP0301BOX PVDF membranes,BIO-RAD,1620174 ,, Deposited data,Deposited data,Deposited data ,, CRISPR screen MAGeCK datasets,BioGRID ORC,1-CUSTOM00000028 ,, Experimental models

(4) HIF2A as a prognostic and clinical therapeutic target in ovarian clear cell carcinomaInternational Journal of CancerJune 1, 2026Mengxin Jiang, Ken Yamaguchi, Kohei Hamada, Yuko Hosoe, Yuka Mise, Sachiko Kitamura, Zhihong Liu, Hairong Wei, Zhenhai Gao, Mana Taki, et al.Equal amounts of protein were resolved on 4%–12% Bis‐Tris gels and transferred to PVDF membranes (#1620177, BIO‐RAD). After blocking, the membranes were incubated overnight at 4°C with primary antibodies against

(5) Protocol for generating iPSC-derived intestinal organoids through dissection to model cystic fibrosis therapiesSTAR ProtocolsJune 1, 2026Alexander J. Stuffer, Barbara Tabak, Joshua Conte, Victoria Momyer, Mercy Chado, Matthew Armstrong, Phillip Wible, Andrey Sivachenko, Normand Allaire, Junjie Lu, et al.,Thermo Fisher Scientific,WG1601BOX XT Tricine Running Buffer,BioRad,1610790 Immun-Blot® PVDF Membrane,BioRad,1620177 10× Tris/CAPS Buffer for Semi-Dry Blotting,BioRad,1610778 SuperSignal™ West Femto Maximum Sensitivity Substrate

(6) Development and Validation of a HAUS-Related Prognostic Signature in Liver Hepatocellular Carcinoma: A Multi-Omics Integration from Pan-Cancer Analysis to Single-Cell ResolutionJ Hepatocell CarcinomaMay 22, 2026Yanli Liu, Kang Liu, Yang Ding, Xiaodong Li, Xuanxuan Shi, Junrui Zhang, Xiangyue Zhang, Weiyao Peng, Jingyi Deng, Jingqi Chenseparated on 10% SDS-PAGE gels and electrotransferred onto polyvinylidene difluoride (PVDF) membranes (#1620177, Bio-Rad). After blocking with 5% skim milk, membranes were incubated overnight at 4 °C with primary

(7) Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neuronsSci AdvMay 22, 2026Laura Sichlinger, Maximilian Hausherr, Sara Guerrisi, Lucia Dutan-Polit, George Chennell, Roland Nagy, Rugile Matuleviciute, Fatema Nasser, Szidonia Farkas, Rosemary A. Bamford, et al.100 V for approximately 90 min, and transferred onto polyvinylidene difluoride membranes (Bio-Rad; 1620177) at 78 mA overnight at 4°C. The membranes were blocked in 5% bovine serum albumin (Sigma-Aldrich; A7906)

(8) SREBP2-activated CNPY3 Phase Separation Promotes Colorectal Cancer by Enhancing MDM2-mediated p53 DegradationInt J Biol SciMay 15, 2026Xue Li, Min-Yue Yin, Yan-Fei Wei, Jie Xing, Qian Zhang, Ye Zong, Shu-Tian Zhang, Si-An Xiedodecyl sulfate-polyacrylamide gel, transferred to a 0.22 μM polyvinylidene fluoride membrane (Bio-Rad, 1620177), blocked in 5% skim milk for 2 h, and incubated with primary antibody overnight at 4 ℃ and the corresponding

(9) Laminaran Attenuates NaCl-Induced Cytotoxicity via ROS Scavenging and Prevents Alteration of Cellular Elastic ModulusMar DrugsMay 15, 2026Hiromi Kurokawa, Atsushi Taninaka, Hirofumi Matsui, Hidemi Shigekawa, Yutaka Kuroki, Makoto M. WatanabePolyacrylamide gel (SDG-545) was from BIO CRAFT Co. Ltd. (Tokyo, Japan), and polyvinylidene difluoride (PVDF) (1620177) membrane was from Bio-Rad Laboratories (Hercules, CA, USA). Can Get Signal® PVDF blocking reagent (NYPBR01),

(10) Uncovering Potential Neutrophil-Related Biomarkers for Early AMI DiagnosisBiology (Basel)May 14, 2026Yuwei Liu, Yun Zhang, Lucheng Wang, Diru Yao, Ebenezeri Erasto Ngowi, Moussa Omorou, Ning Hou, Weibo Dai, Longlong Wang, Guihua Yue, et al.-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA, USA, 1620177). After blocking the membranes with 5% non-fat milk, membranes were incubated overnight at 4 °C with

(11) Mitochondrial Dynamics Participate in an Early Metabolic Adaptation of Glioblastoma Multiforme T98G Cells to Doxorubicin-Induced Chemotherapeutic StressCellsMay 14, 2026Maciej Pudełek, Maksym Pudełek, Julia Przeniosło, Sylwia Kędracka-Krok, Zbigniew Madeja, Jarosław Czyżthe Laemmli protocol and subsequently transferred onto PVDF membranes (Immun-Blot® PVDF Membrane, No. 1620177; Bio-Rad, Hercules, CA, USA [33,54]). Blocking of unspecific staining was performed with skimmed milk/TBST

(12) Gene-specific response to muscle specific kinase agonist antibody in the treatment of congenital myasthenic syndromesBrain CommunMay 14, 2026Kelly Ho, Ofosu Adjei-Afriyie, Ricardo Carmona-Martinez, Rohan Ray, Daniel O'Neil, Joshua Zeldin, Julien Oury, Lieselot De Clercq, Steven J Burden, Bernhardt Vankerckhoven, et al.The proteins were transferred to a pre-activated polyvinylidene difluoride (PVDF) membrane (BioRad 1620177). The membrane was blocked with EveryBlot blocking buffer (BioRad 12010020) for 15 min at RT. The membrane

(13) TRIM25 promotes antiviral innate immune response by stabilizing IRF7 and its nuclear translocationmBioMay 13, 2026Zhen Wu, Yingxuan Cui, Yu He, Tao Wang, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, et al.min, separated by SDS-PAGE, and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, 1620177) using a Bio-Rad semidry transfer system. Membranes were blocked in 5% non-fat milk in TBST for 2–3

(14) A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivoBMC BiolMay 12, 2026Elisabetta D'Avanzo, Amelie Mahr, Nicolas Sauer, Simon Brandt, Ruthger van Roey, Harald Schuhwerk, Philipp Tripal, Benjamin Schmid, Stefanie Brey, Thomas H. Winkler, et al.proteins were transferred to a nitrocellulose membrane (Roth, 4685.1) by wet blot transfer (Bio-Rad, 1620177) with Tris/glycine buffer for 2 h at 300 mA at 4 °C. Antibodies were diluted in 5% dry milk/TBS/0.1%

(15) Activated T cell extracellular vesicle DNA transfer enhances antigen presentation and anti-tumor immunityCancer CellMay 11, 2026Mengying Hu, Di-Ao Liu, Inbal Wortzel, Paul Collier, Theodore M. Nelson, Jonathan Foox, Guojie Zhong, Gabriel Tobias, Tetsuhiko Asao, Linda Bojmar, et al.SDS–PAGE (Invitrogen, XP00100BOX), and then transferred to polyvinylidene difluoride membranes (Bio-Rad, 1620177). The membranes were blocked with 5% bovine serum albumin at room temperature for 1 h and then incubated

(16) HES3-dependent regulatory functions in development and fusion-positive rhabdomyosarcomabioRxivMay 8, 2026Jack Kucinski, Matthew R. Kent, Katherine M. Silvius, Ambuj Kumar, Cenny Taslim, Genevieve C. Kendallprotein was run on a Mini-PROTEAN gel and transferred to a 0.2 μm PVDF membrane (Bio-Rad, 4568033 and 1620177). Blocking occurred with Casein and 0.05% Tween 20 (Fisher Scientific, PI37528). Primary antibodies

(17) Telomerase mRNA-Lipid nanoparticles attenuate neuroinflammation after traumatic brain injury in miceNeurotherapeuticsMay 8, 2026Goknur Kara, Morgan Holcomb, Anjana Tiwari, Hannah Flinn, Trinity Eimer, Austin Marshall, Marissa Burke, Peter Park, Karem A. Court, John P. Cooke, et al.protein separation and electro-transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, cat. #1620177). The membranes were blocked in 5% w/v skim milk powder in PBS-Tween 20 (PBS-T) buffer for 1 h at room

(18) Intranasal CRISPR- lipid nanoparticles targeting MAPK9 reduce neuroinflammation after traumatic brain injurybioRxivMay 2, 2026Goknur Kara, Yaqoob Ali, Jessica López-Espinosa, Peter Park, Morgan Holcomb, Hannah Flinn, Noah Taylor, Tyler Galbraith, Fransisca Leonard, Sonia Villapolgradient and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, cat. #1620177). The membranes were blocked with a 5% w/v skim milk powder solution in PBS-Tween 20 buffer for 1 h

(19) HDAC1 and SATB1 positively regulate immune responses in chicken macrophagesPoultry ScienceMay 1, 2026Bowen Niu, Junda Hu, Zixuan Fan, Zihao Gao, Yuchen Jie, Xinyu Wu, Xingying Chen, Sirui Chen, Li-Wa Shaofor 10 min. Proteins were resolved by SDS-PAGE and electro-transferred to PVDF membranes (Bio-Rad, #1620177). Membranes were blocked for 1 h, then incubated overnight at 4°C with primary antibodies: anti-Myc

(20) Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β–TFEB signaling axisPharmacological ResearchMay 1, 2026Ang Li, Xianglu Xiao, Guopan Liu, Krinos Li, Yixia Ling, Shenglong Deng, Chunzuan Xu, Shu-qin Cao, Jing Wen, Guang Lu, et al.Beyotime). An equal amount of protein was resolved using SDS-PAGE and transferred to a PVDF membrane (1620177; Bio-Rad Laboratories). The membrane was blocked with 5% skimmed milk (1706404; Bio-Rad Laboratories)

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