NI™ (Non-Interfering™) Protein Assay Kit with Albumin Standard from G Biosciences

Product Specs
ItemNI™ (Non-Interfering™) Protein Assay Kit with Albumin Standard
CompanyG Biosciences
Price $266.00Supplier Page View Company Product Page
Catalog Number786-005
Quantity500 Assays
ApplicationsEstimate protein during protein purification, electrophoresis, cell biology, molecular biology, and other research applications. Suitable for protein samples containing common laboratory agents, such as reducing agents ( β-mercaptoethanol, dithiothreitol), chelating agents (EDTA), detergents, amines (Tris), sugars and many other agents. Suitable for samples containing chaotropic agents such as urea, thiourea, guanidine hydrochloride, guanidine thiocyanate, ammonium sulfate, drugs, antibiotics, cobalt, and numerous other agents and extraction buffers. Suitable for determination of protein concentration in cellular fractions, tissue & cell lysates and chromatography purification fractions. Suitable for dilute protein solutions

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G Biosciences

9800 Page Avenue

St. Louis, Missouri 63132

United States

Phone: +800-628-7730
Fax: 314-991-1504

Company Profile

Website: http://www.gbiosciences.com

(1) Synthesis of lipid-linked precursors of the bacterial cell wall is governed by a feedback control mechanism in Pseudomonas aeruginosaNature MicrobiologyFebruary 9, 2024Lindsey S. Marmont, Anna K. Orta, Becca W. A. Baileeves, David Sychantha, Ana Fernández-Galliano, Yancheng E. Li, Neil G. Greene, Robin A. Corey, Phillip J. Stansfeld, William M. Clemons, et al.were boiled at 95 °C for 10 min. Each sample was subjected to the NI protein assay (G Biosciences, 786-005) to determine the protein content in each sample. The lysates (50 μl) were then incubated at 55 °C with

(2) Single-molecule dynamics show a transient lipopolysaccharide transport bridgeNatureNovember 23, 2023Lisa Törk, Caitlin B. Moffatt, Thomas G. Bernhardt, Ethan C. Garner, Daniel Kahnemin. From these samples, the protein concentration was determined with G Biosciences NI Protein Assay (786-005). A volume equivalent of 20 μg of protein was loaded for immunoblots and run on homemade Tris-HCl 4-20%

(3) A role for the Gram-negative outer membrane in bacterial shape determinationProceedings of the National Academy of SciencesAugust 29, 2023Elayne M. Fivenson, Patricia D. A. Rohs, Andrea Vettiger, Marios F. Sardis, Grasiela Torres, Alison Forchoh, Thomas G. BernhardtNoninterfering (NI) Protein Assay [with bovine serum albumin (BSA) protein standard] (G Biosciences catalog no. 786-005). Samples were run on a 15% polyacrylamide gel (LpxC western blots) or 4 to 20% Mini-PROTEAN gels (BioRad

(4) Coordination of bacterial cell wall and outer membrane biosynthesisNatureMarch 1, 2023Hummels KR, Berry SP, Li Z, Taguchi A, Min JK, Walker S, Marks DS, Bernhardt TG.amplitude. Protein concentration was quantified using the Non-Interfering protein assay kit (G-biosciences 786-005). A 10 µg quantity of protein was loaded onto a 10% polyacrylamide–SDS gel and subjected to electrophoresis

(5) A role for the Gram-negative outer membrane in bacterial shape determinationbioRxiv : the preprint server for biologyFebruary 4, 2023Fivenson EM, Rohs PDA, Vettiger A, Sardis MF, Torres G, Forchoh A, Bernhardt TG.Noninterfering (NI) Protein Assay (with bovine serum albumin [BSA] protein standard) (G Biosciences catalog no. 786–005). Samples were run on a 15% polyacrylamide gel (LpxC western blots) or 4–20% Mini-PROTEAN gels (BioRad

(6) High-Throughput Mutagenesis and Cross-Complementation Experiments Reveal Substrate Preference and Critical Residues of the Capsule Transporters in Streptococcus pneumoniaemBioDecember 21, 2021Wan-Zhen Chua, Matthias Maiwald, Kean Lee Chew, Raymond Tzer-Pin Lin, Sanduo Zheng, Lok-To Shamamount of protein in the cell suspension using the NI protein assay kit (G-Biosciences, catalog no. 786-005). Alternatively, normalization of the loading volume based on the OD600 readings of the culture produced

(7) An Essential Membrane Protein Modulates the Proteolysis of LpxC to Control Lipopolysaccharide Synthesis in Escherichia colimBioMay 19, 2020Fivenson EM, Bernhardt TG.noninterfering (NI) protein assay (with bovine serum albumin [BSA] protein standard) (G Biosciences catalog no. 786-005) and was normalized using 1× sample buffer. Samples were run on a 15% polyacrylamide gel and transferred

(8) Proteomic analysis reveals novel insights into tanshinones biosynthesis in Salvia miltiorrhiza hairy rootsScientific ReportsDecember 1, 2019Angela Contreras, Baptiste Leroy, Pierre-Antoine Mariage, Ruddy Wattiez15 min the supernatant was recovered and quantified with Non-interfering agents kit (G-Biosciences 786-005) following the manufacturer’s instructions. Protein trypsin digestion Protein (50 µg) was submitted

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NI™ (Non-Interfering™) Protein Assay Kit with Albumin Standard from G Biosciences

G Biosciences

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NI™ (Non-Interfering™) Protein Assay Kit with Albumin Standard from G Biosciences

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