Kemptide (PKA) Peptide Substrate from Promega

Product Specs
ItemKemptide (PKA) Peptide Substrate
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5601
Quantity1mg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Molecular docking to homology models of human and Trypanosoma brucei ERK8 that identified ortholog-specific inhibitorsPLOS Neglected Tropical DiseasesSeptember 12, 2025Matthew Merski, Zachary B. Mackeyassay. ATP consumption was determined by adding equal volumes of the kinase assay reagent (Promega, Cat# V5601, Madison, WI) following the manufacturer’s protocol. Luminescence was measured using the microplate

(2) A general assay platform to study protein pharmacology using ligand-dependent structural dynamicsNat CommunMay 10, 2025Daniel A. Ciulla, Patricia K. Dranchak, Mahesh Aitha, Renier H. P. van Neer, Divia Shah, Ravi Tharakan, Kelli M. Wilson, Yuhong Wang, John C. Braisted, James Inglese(A214): 88.66%. Kemptide is also commercially available from several sources, including Promega (Cat #V5601), Anaspec (Cat#AS-22594), and Thermo Scientific (Cat# J60591-#0). Abltide is also commercially available

(3) Interruption of p38MAPK-MSK1-CREB-MITF-M pathway to prevent hyperpigmentation in the skinInternational Journal of Biological SciencesFebruary 17, 2024Song-Hee Kim, Jiyeon Lee, Jihye Jung, Ga Hyun Kim, Cheong-Yong Yun, Sang-Hun Jung, Bang Yeon Hwang, Jin Tae Hong, Sang-Bae Han, Jae-Kyung Jung, et al.Kinase assay To determine cellular PKA activity, lysates of B16F0 cells were reacted with kemptide (Promega, V5601) as PKA-specific peptide substrate, and 10 μCi [γ-32P]-ATP (Perkin Elmer, NEG002A) as a probe for 30

(4) Targeting phosphorylation circuits on CREB and CRTCs as the strategy to prevent acquired skin hyperpigmentationInternational Journal of Biological SciencesJanuary 1, 2024Song-Hee Kim, Changseon Na, Cheng-Yong Yun, Jun Gu Kim, Seung Tae Baek, Hyun Jin An, Jae Duk Lee, Seung Wha Lee, Jae-Kyung Jung, Bang Yeon Hwang, et al.cells or catalytically active rhPKA (Sigma-Aldrich, 14-440) were reacted with 83 μM Kemptide (Promega, V5601) as substrate in the presence of 10 μCi [γ-32P]-ATP (Perkin Elmer, NEG002A) at 30°C for 30 min. The

(5) Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMPEMBO reportsAugust 3, 2022Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A.μl of the same buffer supplemented with 70 μM ATP and 0.3 mM Kemptide, a PKA substrate (LRRASLG; # V5601 Promega) and incubated for 0.5 h at 30°C in 96‐well plate. The ATP hydrolysis was detected upon the

(6) Kinase‐Catalyzed Biotinylation of Peptides, Proteins, and LysatesCurrent Protocols in Chemical BiologyMarch 1, 2012Chamara Senevirathne, Keith D. Green, Mary Kay H. Pflumconcentration of 20 μM in the reaction. For the PKA peptide substrate (LRRASLG, kemptide, Promega, cat. No. V5601), we created a 200 μM stock solution using deionized, purified water or kinase buffer. For the CK2 peptide

(7) Multiplex Enzyme Assays and Inhibitor Screening by Mass SpectrometryJ Biomol ScreenSeptember 1, 2010Rakesh Rathore, Patrick Pribil, Jay J. Corr, William L. Seibel, Artem Evdokimov, Kenneth D. Greis(St. Louis, MO). The catalytic subunit of cAMP-dependent protein kinase (PKA, V5161) and Kemptide (V5601) were purchased from Promega (Madison, WI). Ammonium monobasic phosphate and formic acid were from EMD

(8) Development of an Inhibitor Screening Platform via Mass SpectrometryJ Biomol ScreenDecember 1, 2008Rakesh Rathore, Jay Corr, George Scott, Pauline Vollmerhaus, Kenneth D. GreisMETHODS Materials The catalytic subunit of cAMP-dependent protein kinase (PKA, V5161) and kemptide (V5601) were purchased from Promega (Madison, WIi). α-Cyano-4-hydroxycinnamic acid (4-HCCA), staurosporine,

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