HaloTag Standard Protein from Promega

Product Specs
ItemHaloTag Standard Protein
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG4491
Quantity30microg
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genesMol CellMarch 19, 2026Steven Ingersoll, Abby Trouth, J. Carlos Angel, Xinlong Luo, Axel Espinoza, Joey Wen, Chengjie Zhu, Joseph Tucker, Kalkidan Astatike, Christopher J. Phiel, et al.27-0843-01 Recombinant PRC2 complex,Active Motif,Cat# 31387 HaloTag® Standard Protein,Promega,Cat# G4491 FluoroBrite DMEM,Life Technologies,Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand,Promega,Cat# GA1110 L-Glutathione,Sigma,Cat#

(2) Quantifying Intracellular Distributions of HaloTag-Labeled Proteins With SDS-PAGE and Epifluorescence MicroscopyBio ProtocJuly 20, 2025Julia Shangguan, Ronald S. RockLaminin (Sigma-Aldrich, catalog number: CC095-M) 6. HaloTag standard protein (Promega, catalog number: G4491) 7. SuperSignal West Femto chemiluminescent substrate (Thermo Scientific, catalog number: 34094) 8.

(3) DELTA: a method for brain-wide measurement of synaptic protein turnover reveals localized plasticity during learningNature NeuroscienceMarch 31, 2025Boaz Mohar, Gabriela Michel, Yi-Zhi Wang, Veronica Hernandez, Jonathan B. Grimm, Jin-Yong Park, Ronak Patel, Morgan Clarke, Timothy A. Brown, Cornelius Bergmann, et al.Chemistry36 initiative (https://dyes.janelia.org/) or can be purchased from Promega. Purified HT protein (G4491) was from Promega. Pluronic F-127 (P3000MP), anhydrous dimethylsulfoxide (DMSO; D12345), N,N,N′,N′-tetramethylethylenediamine

(4) Hundreds of myosin 10s are pushed to the tips of filopodia and could cause traffic jams on actineLifeOctober 31, 2024Shangguan J, Rock RS.samples were stored at –80°C. SDS gel analysis for Myo10 quantitation HaloTag standard protein (Promega G4491) samples, with 0.5 µM TMR-HaloLigand, were prepared for final masses of 1.25 ng, 2.5 ng, 5 ng, 10 ng,

(5) Distinct mobility patterns of BRCA2 molecules at DNA damage sitesNucleic Acids ResearchAugust 12, 2024Maarten W Paul, Jesse Aaron, Eric Wait, Romano M Van Genderen, Arti Tyagi, Hélène Kabbech, Ihor Smal, Teng-Leong Chew, Roland Kanaar, Claire Wymansegmented 53BP1 focus. Quantification of cellular concentration of BRCA2 HaloTag-GST (61 kDa) (Promega, G4491) was labelled with JF646 for 4 h at 4°C on ice with 3× excess HaloTag ligand (buffer: 1× PBS, 20% glycerol,

(6) HaloTag display enables quantitative single‐particle characterisation and functionalisation of engineered extracellular vesiclesJournal of Extracellular VesiclesJuly 5, 2024Roxana E. Mitrut, Devin M. Stranford, Beth N. DiBiase, Jonathan M. Chan, Matthew D. Bailey, Minrui Luo, Clare S. Harper, Thomas J. Meade, Muzhou Wang, Joshua N. Leonardprotein loading on EVs, several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labelled with ligand and run in duplicate to generate a calibration curve. Samples and the calibration

(7) HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesiclesbioRxiv : the preprint server for biologyMay 3, 2024Mitrut RE, Stranford DM, DiBiase BN, Chan JM, Bailey MD, Luo M, Harper CS, Meade TJ, Wang M, Leonard JN.protein loading on EVs, several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve. Samples and the calibration

(8) Pushed to the edge: hundreds of Myosin 10s pack into filopodia and could cause traffic jams on actinbioRxivJuly 4, 2023Julia Shangguan, Ronald S. Rockmoved to ice post-spin and lysed in RIPA buffer containing 1 mM PMSF. Halo Standard Protein (Promega, G4491) samples were prepared for final masses of 1 ng, 5 ng, and 10 ng and lysate samples containing 40,000

(9) Fluorogenic Protein Probes with Red and Near‐Infrared Emission for Genetically Targeted Imaging**Chemistry – A European JournalNovember 11, 2020Sylvestre P. J. T. Bachollet, Cyril Addi, Nicolas Pietrancosta, Jean‐Maurice Mallet, Blaise Dumatpurchased from SigmaAldrich or TCI and were used as received. Purified GST-Halotag protein (product #G4491) was purchased from Promega Corp. (Madison, Wi, USA) at a concentration of 2.55 – 2.63 mg/ml depending

(10) Quantitative Real-Time Imaging of Glutathione with Subcellular ResolutionAntioxidants & Redox SignalingJune 1, 2019Xiqian Jiang et al.HaloRT probe HaloRT ligand (400 lM, 200 lL) was incubated with purified His-HaloTag protein (Promega, G4491, 6.5 mg/mL, 50 lL) for 1 hour at room temperature in phosphate-buffered saline (PBS). The reaction mixture

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