E.coli Poly (A) Polymerase from New England Biolabs

Product Specs
ItemE.coli Poly (A) Polymerase
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberM0276S
Quantity100 units
Concentration20,000 units/ml
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 50 µl volume in 10 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.Sensitivity Assay Kit (Thermo Fisher, Q32852) post-IVT. The Escherichia coli Poly(A) Polymerase Kit (NEB, M0276S) was then used to perform polyadenylation. Post-polyadenylation, the Qubit assays were performed three

(2) Robust CD8+ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFront ImmunolApril 1, 2026Kexin Li, Xiaoya Li, Cong Zhang, Suli Dai, Lei Li, Jun Wang, Hongtao Zhang, Lianmei ZhaoNew England Biolabs, USA) to form Cap1 structures. Polyadenylation was performed using E. coli Poly(A) Polymerase (#M0276L, New England Biolabs, USA). The synthesized mRNAs underwent purification with the Monarch RNA Cleanup

(3) A CRISPR-Cas13d cancer therapeutic enables selective elimination of uveal melanomaMolecular Therapy OncologyMarch 19, 2026Daniel Stauber, Lucas Sosnick, Yitong Ma, Sopida Pimcharoen, Atip Lawanprasert, Niren Murthy, David Myung, Lei S. QiPoly(A) tails were synthesized post-transcription using Escherichia coli poly(A) polymerase (NEB, #M0276). The sgRNA was synthesized by IVT using HiScribe T7 Quick T7 High Yield Synthesis Kit (NEB, #E2050).

(4) A nanopore-based HIV-1 reference epitranscriptomeNucleic Acids ResMarch 19, 2026Michael S Bosmeny, Adrian A Pater, Li Zhang, Lydia L Larkai, Beverly E Sha, Zidi Lyu, Masad J Damha, João I Mamede, Keith T Gagnonpolyadenylation reactions were performed using Escherichia coli Poly(A) Polymerase (New England Biolabs, M0276L). Briefly, 1000 ng of rRNA-depleted RNA was treated with 0.375 U/µl E. coli Poly(A) Polymerase in 1×

(5) Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary CellsMicromachines (Basel)March 15, 2026Hyun Woo Sung, Soojung Claire Hurunmutated template. A Poly-(A) tail of approximately 100 nt was added using E. coli Poly(A) Polymerase (cat#M0276L, New England Biolabs, This page contains the following errors:) to the mRNA product. After each step,

(6) Bacterial Schlafen proteins mediate phage defenceNature MicrobiologyMarch 9, 2026Veronica Perez Taboada, Yimo Wu, Riley Cassidy, Kirill E. Medvedev, Luuk Loeff, Anna Nemudraia, Artem Nemudryikit (NEB, T2030L). PNK-treated RNA samples were polyadenylated using E. coli Poly(A) polymerase (NEB, M0276S) in a reaction buffer (50 mM Tris–HCl, pH 8.0, 250 mM NaCl, 10 mM MgCl2, 1 mM ATP and murine RNase inhibitor

(7) Nanopore direct RNA sequencing (DRS) of MS2 bacteriophages in E. coli throughout its life cycles reveals a complex transcriptional activity to control and maintain its growthVirology JournalMarch 6, 2026Ni Ni, Gaetan Burgioand cooled on ice for one min before polyadenylating RNAs using the E. coli poly(A) polymerase (NEB M0276S [NEW ENGLAND Biolabs]). Briefly, 10 µg RNA, 5 units poly(A) polymerase, 2 µL 10X reaction buffer, and

(8) Single intramuscular injection of self-amplifying RNA of Nppa to treat myocardial infarctionScienceMarch 5, 2026Kaiyue Zhang, Hongyan Tao, Dashuai Zhu, Zhang Yue, Shiqi Hu, Yiping Wu, Na Yan, Yilan Hu, Shuo Liu, Mengrui Liu, et al.were capped with Faustovirus Capping Enzyme (M20081, NEB) and tailed with E. coli Poly(A) Polymerase (M0276, NEB). After each step, the reaction products were purified using Monarch RNA Cleanup Kit (T2050, NEB).

(9) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. GladfelterLiCl, RNA poly(A) tails were added to the Cap-1 mRNAs according to manufacturer specifications (NEB, M0276L). Reactions were then purified a second time with 2.5 M LiCl precipitation. The RNA concentration was

(10) An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencingSTAR ProtocolsMarch 1, 2026Kamil Więcek, Janusz Wiśniewski, Heng-Chang ChenE. coli Poly(A) Polymerase reaction buffer [supplied in the package with E. coli Poly(A) Polymerase],New England Biolabs,M0276S 100 units E. coli Poly(A) Polymerase,New England Biolabs,M0276S 10 mM ATP [supplied in the package

(11) Rapid detection of human and animal respiratory viruses using Microbe Finder (MiFi®)Frontiers in MicrobiologyFebruary 25, 2026Marcos R. Ribeiro-Junior, Kitty F. Cardwell, Daniele Nascimento, Andres S. Espindola, Akhilesh Ramachandran, Sushim K. Gupta, Donna Tyungurequired poly(A) tails, we performed 3’ polyadenylation using E. coli Poly(A) Polymerase (NEB, Cat. No. M0276L) following Oxford Nanopore’s recommended protocol. Briefly, 1–10 μg of rRNA-depleted RNA was incubated

(12) Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunitybioRxivJanuary 29, 2026Xiaolei Cai, Min Chen, Guoshuai Cao, Nicholas Asby, Derek Elli, Haley Gula, Vlad Nicolaescu, Duy-Thuc Nguyen, Xiaodan Huang, Tanushree Dangi, et al.mRNA Cap 2´-O-Methyltransferase (NEB #M0366), followed by poly (A) tailing using E. coli Poly(A) Polymerase (NEB #M0276). The resulting mRNAs were purified using the Monarch® RNA Cleanup Kit (NEB #T2050), and the integrity

(13) Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decayNucleic Acids ResearchJanuary 29, 2026Max Müller, Lena Sophie Tittel, Elisabeth Petfalski, Kaushik Viswanathan Iyer, Alina-Andrea Kraft, Stefan Pastore, Tamer Butto, Marie-Luise Winzwas conducted according to the manual “Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase” (NEB #M0276). After 30 min of incubation at 37°C, ethylenediaminetetraacetic acid was added to stop the reaction

(14) The RNA exosome maintains cellular RNA homeostasis by controlling transcript abundance in the brainCell ReportsJanuary 27, 2026Lauryn A. Higginson, Xingjun Wang, Kevin He, Maggie Torstrick, Minhoo Kim, H.R. Sagara Wijeratne, Avery M. Runnebohm, Bérénice A. Benayoun, Adam MacLean, Guillaume F. Chanfreau, et al.heads using TRIzol reagent and in vitro polyadenylated with E. coli Poly(A) Polymerase (NEB, catalog #M0276S). RNA libraries were prepared from 500ng of polyadenylated RNAs using the PCR-cDNA barcoding kit (Oxford Nanopore Technologies

(15) Circularization of 23S rRNA but not 16S rRNA within archaeal ribosomesGenome BiologyJanuary 27, 2026Ling-Dong Shi, Petar I. Penev, Amos J. Nissley, Dipti D. Nayak, Rohan Sachdeva, Jamie H. D. Cate, Jillian F. Banfielddid not pre-deplete rRNAs. In brief, all the RNA were polyadenylated using poly(A) polymerase (NEB, M0276L) and purified by GeneJET RNA Cleanup and Concentration Micro Kit (ThermoFisher, K0841). The poly(A)-tailed

(16) In vivo AGO-APP for cell-type- and compartment-specific miRNA profiling in the mouse brainCell Reports MethodsJanuary 26, 2026Surbhi Kapoor, Andrea Erni, Francesca Vincenzi, Beatrice Tessier, Vasika Venugopal, Gunter Meister, Alexandre Favereaux, Harold Cremer, Christophe Beclin,Tocris Bioscience,3525 D-Gluconate,Merck,S2054 HEPES,Merck,H4034 EGTA,Merck,324626 Proteinase K (PCR grade),Roche product,3115801001 E. coli poly(A) polymerase,New England Biolabs,M0276S GoTaq® G2 Flexi DNA Polymerase,Promega,M7808 "PHUSION HIGH-FIDELITY DNA POLY MERASE, 100U",Thermo Fisher Scientific

(17) Integrative analysis of nanopore direct RNA sequencing data reveals a global impact of pseudouridylation on m6A and m5C modificationsnpj Precision OncologyJanuary 22, 2026Mohit Bansal, Anamika Gupta, Jane Ding, Anirban Kundu, Katherine Marlow, Andrew Gibson, Zhangli Su, Sunil Sudarshan, Han-Fei Dingpoly-A mRNA sequencing. We first added a poly-A tail to the total RNA using poly-A tailing kit (NEB M0276) and then sequenced it using Flongle flow cells. Human rRNA sequences (NR_003286, NR_145820.1, X03205.1,

(18) Dynamont: A comprehensive cross-species comparison of ONT segmentation toolsGigaScienceJanuary 21, 2026Jannes Spangenberg, Christian Höner zu zu Siederdissen, Winfried Goettsch, Lennart Köhler, Liz Maria Luke, Kai Papenfort, Manja Marzto the RNAs by incubation of 400 ng RNA with 300 nM ATP and 2.5 U E. coli Poly(A) Polymerase (NEB; M0276) for 30 minutes at 37\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}

(19) Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cellsCommunications BiologyJanuary 19, 2026Jorik Frederik Bot, Zhihan Zhao, Mengyuan Li, Darnell Kammeron, Peng Shang, Niels Geijsenpoly-A tail, we polyadenylated the rRNA depleted RNA with 0.25 units/µL E. coli Poly(A) polymerase (NEB, M0276L) in the presence of 1 mM ATP and 1 unit/μL RNasin® (Promega, N2515) for 30 min at 37 °C. Afterwards,

(20) Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulationFrontiers in Veterinary ScienceJanuary 14, 2026Fernanda Schneberger, Alessandra Bridi, Priscila Assis Ferraz, José Roberto Quirino de Oliveira, Lorena Calixto Munhoz, Juliano Rodrigues Sangalli, Cibele Maria Prado, Ludimila Cardoso Zoccal Janini, Felipe Perecin, Juliano Coelho da Silveira, et al.ng total RNA, 10 μM Adapter Primer, and Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase Kit (M0276, New England Biolabs, Ipswich, United States) at 37 °C for 60 min, then 70 °C for 5 min. Followed by

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