NEBNext dA-Tailing Module from New England Biolabs

Product Specs
ItemNEBNext dA-Tailing Module
CompanyNew England Biolabs
Price $459.00 Inquire
Catalog NumberE6053L
Quantity100 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChewGenome BiolApril 27, 2026Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, et al.reaction buffer and 10 μL of Klenow fragment (New England Biolabs, NEBNext dA-Tailing Module, Cat. No. E6053L) were added. The reaction volume was adjusted to 250 μL with nuclease-free water, and the mixture was

(2) A plastic EMP1⁺ to LGR5⁺ cell state conversion as a bypass to KRAS-G12D pharmacological inhibition in metastatic colorectal cancerCancer DiscoveryFebruary 6, 2026Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.µL 1× BB, once with 100 µL 1× NEBuffer 2, and then resuspended with 100 µL dATP attachment mix (NEB, E6053L; 10 µL 10× NEBNext dA-Tailing Reaction Buffer, 5 µL 5 U/µL Klenow-exo and water up to 100 µL). The mixture

(3) Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1iScienceJanuary 16, 2026Sukjae Han, Joon Young Park, Yong Hee Han, Ina Bang, Hyeonwoo Mun, Ok Kyung Lee, Ji Sung Hyung, Jeong-Geol Na, Eun Yeol Lee, Donghyuk Kim, et al.NEBNext® End Repair Module,New England Biolabs,Cat#E6050L NEBNext® dA-Tailing Module,New England Biolabs,Cat#E6053S Quick Ligation™ Kit,New England Biolabs,Cat#M2200S PreCR® Repair Mix,New England Biolabs,Cat#M0309S Lambda Exonuclease

(4) Genome-Wide Identification of Tissue-Specific Fitness Genes in Murine Models of Staphylococcus aureus InfectioniScienceNovember 28, 2025Sally W. Yousief, Nader Abdelmalek, Martin S. Bojer, Yibing Ma, Priscila R. Guerra, Sajid Nisar, John E. Olsen, Bianca PagliettiNEBNext End Repair module,New England Biolab,Cat# E6050 NEBNext dA-Tailing Module,New England Biolab,Cat# E6053 NEBNext Quick Ligation Module,New England Biolab,Cat# E6056 KAPA HiFi HotStart ReadyMix,KAPA Biosystems

(5) BMAL1-TRIM28 represses transposable elements independently of CLOCK in pluripotent cellsNature CommunicationsSeptember 10, 2025Amador Gallardo, Efres Belmonte-Reche, María Marti-Marimon, Joan Domingo-Reinés, Guillermo Peris, Lourdes López-Onieva, Iván Fernández-Rengel, Jiajun Xie, Pablo Tristán-Ramos, Nicolas Bellora, et al.(New England Biolabs, #E6050L) and A-tailed with the NEBNext dA-Tailing Module (New England Biolabs, #E6053L). After adapters ligation, libraries were indexed and amplified with NEBNext UDI primer pairs (New England Biolabs

(6) In vivo prime editing rescues alternating hemiplegia of childhood in miceCellAugust 7, 2025Alexander A. Sousa, Markus Terrey, Holt A. Sakai, Christine Q. Simmons, Elena Arystarkhova, Natalia S. Morsci, Laura C. Anderson, Jun Xie, Fabian Suri-Payer, Linda C. Laux, et al.NEBNext End Repair Module,New England BioLabs,Cat#E6050L NEBNext dA-Tailing Module,New England BioLabs,Cat#E6053L NEBNext Quick Ligation Module,New England BioLabs,Cat#E6056L NEBNext Multiplex Oligos for Illumina

(7) Interpreting regulatory mechanisms of Hippo signaling through a deep learning sequence modelCell GenomApril 9, 2025Khyati Dalal, Charles McAnany, Melanie Weilert, Mary Cathleen McKinney, Sabrina Krueger, Julia Zeitlingercommercial assays ,, End Repair Module,New England Biolabs,E6050S dA-Tailing Module,New England Biolabs,E6053S Quick Ligation Kit,New England Biolabs,M2200S Monarch DNA Gel Extraction Kit,New England Biolabs,T1020 Monarch

(8) Mutation of the LRG1 Rho-GAP gene is responsible for the hyper branching C-variant phenotype in the quorn mycoprotein fungus Fusarium venenatum A3/5Fungal Biol BiotechnolMarch 24, 2025John Connell, Helen J. Bates, Ivey Geoghegan, Fiona Wilson, Richard J. Harrison, R. Jordan Priceconstructed with a PCR-free method using NEBNext End Repair (NEB, E6050S), NEBNext dA-tailing (NEB, E6053S) and Blunt T/A ligase (NEB, M0367S) modules. Libraries were multiplexed and sequenced using Illumina

(9) Ultraviolet damage and repair maps in Drosophila reveal the impact of domain-specific changes in nucleosome repeat length on repair efficiencyGenome ResearchFebruary 14, 2025Benjamin Morledge-Hampton, Kathiresan Selvam, Manish Chauhan, Alan G. Goodman, John J. WyrickWyrick 2020). The genomic DNA samples were sonicated, end-repaired (NEB E6050L) and dA-tailed (NEB E6053L), ligated to F1 adapter, and treated with terminal transferase (NEB M0315L). The DNA was then digested

(10) Elf1 promotes transcription-coupled repair in yeast by using its C-terminal domain to bind TFIIHNature CommunicationsJuly 23, 2024Kathiresan Selvam, Jun Xu, Hannah E. Wilson, Juntaek Oh, Qingrong Li, Dong Wang, John J. WyrickDiagenode Biorupter 300) to an average size of ∼400 bp, end-repaired (NEB, E6050L), and dA-tailed (NEB, E6053L) using the indicated NEB kits. DNA was then ligated to the double-stranded adapter DNA trP1 using Quick

(11) High resolution long-read telomere sequencing reveals dynamic mechanisms in aging and cancerNature CommunicationsJune 18, 2024Tobias T. Schmidt, Carly Tyer, Preeyesh Rughani, Candy Haggblom, Jeffrey R. Jones, Xiaoguang Dai, Kelly A. Frazer, Fred H. Gage, Sissel Juul, Scott Hickey, et al.inactivated at 65 °C for 20 min. Digested DNA was treated with 45 U Klenow Fragment (3’ → 5’ exo-) (NEB, E6053) in a final volume of 250 µL of 1x NEBNext dA-tailing reaction buffer (NEB), split evenly into five

(12) Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearanceVirus EvolutionJanuary 22, 2024Vasanthi Avadhanula, Daniel Paiva Agustinho, Vipin Kumar Menon, Roy F Chemaly, Dimpy P Shah, Xiang Qin, Anil Surathu, Harshavardhan Doddapaneni, Donna M Muzny, Ginger A Metcalf, et al.Qiagen). Next, 5 μl of 10X AT buffer and 3 μl of Klenow enzyme from NEBNext® dA-Tailing (AT) Module (E6053L, NEB) was added to the sample. The AT reaction was incubated at 37°C for 30 minutes. After incubation,

(13) Revisiting chromatin packaging in mouse spermGenome researchDecember 27, 2023Yin Q, Yang CH, Strelkova OS, Wu J, Sun Y, Gopalan S, Yang L, Dekker J, Fazzio TG, Li XZ, et al.purified by PCI and ethanol precipitation and was subjected to dA-tailing using Klenow fragment (NEB E6053). Reactions were incubated for 30 min at 37°C and immediately subjected to adapter ligation as per the

(14) Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activationDevelopmental CellOctober 9, 2023Kaelan J. Brennan, Melanie Weilert, Sabrina Krueger, Anusri Pampari, Hsiao-yun Liu, Ally W.H. Yang, Jason A. Morrison, Timothy R. Hughes, Christine A. Rushlow, Anshul Kundaje, et al.assays ,, End Repair Module,New England Biolabs,Cat# E6050S dA-Tailing Module,New England Biolabs,Cat# E6053S Quick Ligation Kit,New England Biolabs,Cat# M2200S High Throughput Library Prep Kit,KAPA Biosystems,Cat#

(15) Reconstructing mutational lineages in breast cancer by multi-patient-targeted single-cell DNA sequencingCell GenomicsJanuary 11, 2023Jake Leighton, Min Hu, Emi Sei, Funda Meric-Bernstam, Nicholas E. NavinMix,Roche,Cat# KK2602 NEBNext end repair,NEB,Cat# E6050L Quick Ligation,NEB,Cat# E6056L dA-tailing,NEB,Cat# E6053L NEBNext HiFi2x PCRmix,NEB,Cat# M0541L Nimblegen SeqCap EZ Exome V2 kit,Roche,Cat# 05860482001 Mission

(16) Automated next-generation profiling of genomic alterations in human cancersNature CommunicationsDecember 1, 2022Laurel A. Keefer, James R. White, Derrick E. Wood, Kelly M. R. Gerding, Kenneth C. Valkenburg, David Riley, Christopher Gault, Eniko Papp, Christine M. Vollmer, Amy Greer, et al.New England Biolabs (Ipswich, MA, USA) end-repair, A-tailing, and adapter ligation modules (catalog numbers E6050, E6053, and E6056, respectively). Finally, genomic libraries were amplified and captured using the Agilent

(17) Precision digital mapping of endogenous and induced genomic DNA breaks by INDUCE-seqNature CommunicationsDecember 1, 2022Felix M. Dobbs, Patrick van Eijk, Mick D. Fellows, Luisa Loiacono, Roberto Nitsch, Simon H. Reedthen washed three times in 1x CutSmart® Buffer and A-tailed using NEBNext® dA-Tailing Module (NEB, E6053L) in a final volume of 50 µL at 37 °C for 30 mins. A-tailed cells were washed three times in 1x CutSmart® buffer

(18) Structural basis for Cas9 off-target activityCellOctober 27, 2022Martin Pacesa, Chun-Han Lin, Antoine Cléry, Aakash Saha, Pablo R. Arantes, Katja Bargsten, Matthew J. Irby, Frédéric H.-T. Allain, Giulia Palermo, Peter Cameron, et al.13323 High Sensitivity DNA Bioanalyzer Kit,Agilent,Cat# 5067–4626 NEBNext^ dA-tailing Kit,NEB,Cat# E6053L NEBNext^ Ultra End Repair / dA-tailing module,NEB,Cat# E7442L NEBNext^ Ultra Ligation Kit,NEB,Cat#

(19) Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elementsNature GeneticsOctober 4, 2022Sofia Battaglia, Kevin Dong, Jingyi Wu, Zeyu Chen, Fadi J. Najm, Yuanyuan Zhang, Molly M. Moore, Vivian Hecht, Noam Shoresh, Bradley E. Bernstein35 μl each and mixed with 4 μl 10× NEBNext dA-Tailing Reaction Buffer and 3 μl Klenow Fragment (NEB, E6053). Reactions were incubated for 30 min at 37 °C and immediately subjected to nanopore adapter ligation.

(20) Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individualsbioRxivSeptember 22, 2022Harsha Doddapaneni et al.(129114, Qiagen). Next, 5 μl of 10X AT buffer and 3 μl of Klenow enzyme from NEBNext® dA-Tailing Module (E6053L, NEB) was added to the sample. The AT reaction was incubated at 37°C for 30 minutes. After incubation,

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