p-Nitrophenyl Phosphate (PNPP) from New England Biolabs

Product Specs
Itemp-Nitrophenyl Phosphate (PNPP)
CompanyNew England Biolabs
Price $148.00 Inquire
Catalog NumberP0757L
Quantity5 ml
ApplicationsProteomics & Glycomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) SHP2 ablation mitigates osteoarthritic cartilage degeneration by promoting chondrocyte anabolism through SOX9The FASEB JournalSeptember 15, 2024Lijun Wang, Huiliang Yang, Changwei Wang, Mingliang Wang, Jiahui Huang, Thedoe Nyunt, Camilo Osorio, Shi‐Yong Sun, Maurizio Pacifici, Véronique Lefebvre, et al.antibody. The phosphatase activity of SHP2 was measured as described37 using pNPP substrates (NEB, Cat. #P0757s) and was presented as a relative fold change compared to controls. DNA constructs, transfection, and

(2) SHP2 Ablation Mitigates Osteoarthritic Cartilage Degeneration by Promoting Chondrocyte Anabolism Through SOX9FASEB JSeptember 15, 2024Lijun Wang, Huiliang Yang, Changwei Wang, Mingliang Wang, Jiahui Huang, Thedoe Nyunt, Camilo Osorio, Shi-yong Sun, Maurizio Pacifici, Véronique Lefebvre, et al.antibody. The phosphatase activity of SHP2 was measured as described37 using pNPP substrates (NEB, Cat. #P0757s) and was presented as a relative fold change compared to controls. DNA constructs, transfection, and

(3) Methyl vinyl ketone and its analogs covalently modify PI3K and alter physiological functions by inhibiting PI3K signalingThe Journal of biological chemistryMarch 1, 2024Morimoto A, Takasugi N, Pan Y, Kubota S, Dohmae N, Abiko Y, Uchida K, Kumagai Y, Uehara T.measured by BCA protein assay. Cell lysates (150 μg) were incubated with 10 mM pNPP (New England Biolabs, P0757S) and 0.1 mg/ml bovine serum albumin at 37 °C for 10 min. The reaction was terminated under alkaline

(4) Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In VitroACS omegaAugust 22, 2023Jolly JT, Cheatham TC, Blackburn JS.DiFMUP, 6,8-difluoro-4-methylumbelliferyl phosphate (Thermo Fisher, D6567); pNPP, p-nitrophenyl phosphate, disodium salt (NEB, P0757S); DDAOP, 9H-(1,3-dichloro-9, 9-dimethylacridin-2-ona-7-yl)-phosphate (Bimol, ABD-11629); phospho-serine,

(5) Plk4 Is a Novel Substrate of Protein Phosphatase 5International Journal of Molecular SciencesJanuary 19, 2023Edit Ábrahám, Zsuzsánna Réthi-Nagy, Péter Vilmos, Rita Sinka, Zoltán Lipinszkiperformed in 1 mL phosphatase buffer containing 2 μg purified proteins and 2,5 μL pNPP (500 mM, cat # P0757S, New England Biolabs, Ipswich, MA, USA) at 30 °C for 10 min, then the absorbance of the liberated p-nitrophenol

(6) Shortened lifespan induced by a high-glucose diet is associated with intestinal immune dysfunction in Drosophila sechelliaJournal of Experimental BiologyNovember 1, 2022Maiko Abe, Takumi Kamiyama, Yasushi Izumi, Qingyin Qian, Yuma Yoshihashi, Yousuke Degawa, Kaori Watanabe, Yukako Hattori, Tadashi Uemura, Ryusuke Niwadescribed (Pereira et al., 2018). To measure IAP activity, we used para-nitrophenyl phosphate (pNPP; P0757S, New England Biolabs, Ipswich, MA, USA), a general phosphatase chromogenic substrate, as previously

(7) Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domaineLifeOctober 20, 2022Iain M Hay, Katie E Mulholland, Tiffany Lai, Stephen C Graham, Hayley J Sharpe, Janet E DeanekDa-FITC-dextran,Sigma,Cat#FD250S-100MG, "Chemical compound, drug",Para-Nitrophenol-phosphate (pNPP),New England Biolabs,Cat#P0757, "Chemical compound, drug",IPTG,Generon,Cat#GEN-S-02122, "Chemical compound, drug",D-biotin,Sigma,Cat#B4639, "Chemical

(8) Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domainElifeOctober 20, 2022Iain M Hay, Katie E Mulholland, Tiffany Lai, Stephen C Graham, Hayley J Sharpe, Janet E DeanekDa-FITC-dextran,Sigma,Cat#FD250S-100MG, "Chemical compound, drug",Para-Nitrophenol-phosphate (pNPP),New England Biolabs,Cat#P0757, "Chemical compound, drug",IPTG,Generon,Cat#GEN-S-02122, "Chemical compound, drug",D-biotin,Sigma,Cat#B4639, "Chemical

(9) PI3K drives the de novo synthesis of coenzyme A from vitamin B5NatureAugust 1, 2022Dibble CC, Barritt SA, Perry GE, Lien EC, Geck RC, DuBois-Coyne SE, Bartee D, Zengeya TT, Cohen EB, Yuan M, et al.agarose beads were incubated with 20 μl of 50 mM para-nitrophenyl phosphate (PNPP) (New England Biolabs, P0757L) in reaction buffer at 30 °C for 30 min with regular mixing of beads and solution. The reaction was

(10) Stereospecific lasofoxifene derivatives reveal the interplay between estrogen receptor alpha stability and antagonistic activity in ESR1 mutant breast cancer cellseLifeMay 16, 2022David J Hosfield, Sandra Weber, Nan-Sheng Li, Madline Sauvage, Carstyn F Joiner, Govinda R Hancock, Emily A Sullivan, Estelle Ndukwe, Ross Han, Sydney Cush, et al.frozen at –80 °C for at least 12 hr. Thawed plates were incubated with p-nitrophenyl phosphate (NEB, #P0757L) for 1–3 hr at 37 °C. Absorbance was read at 405 nm on a BioTek Cytation 5 plate reader. These assays

(11) Stereospecific lasofoxifene derivatives reveal the interplay between estrogen receptor alpha stability and antagonistic activity in ESR1 mutant breast cancer cellsElifeMay 16, 2022David J Hosfield, Sandra Weber, Nan-Sheng Li, Madline Sauvage, Carstyn F Joiner, Govinda R Hancock, Emily A Sullivan, Estelle Ndukwe, Ross Han, Sydney Cush, et al.frozen at –80 °C for at least 12 hr. Thawed plates were incubated with p-nitrophenyl phosphate (NEB, #P0757L) for 1–3 hr at 37 °C. Absorbance was read at 405 nm on a BioTek Cytation 5 plate reader. These assays

(12) Cdc14 spatiotemporally dephosphorylates Atg13 to activate autophagy during meiotic divisionsJournal of Cell BiologyMay 2, 2022Wenzhi Feng, Orlando ArgÃ¼ello-Miranda, Suhong Qian, Fei Wang1:5,000; Bio-Rad). In vitro Cdc14 phosphatase activity assay 500 mM p-nitrophenyl phosphate (pNPP; NEB, P0757S) substrate solution was diluted to 20 mM in phosphatase reaction buffer (PRB; 20 mM Tris, pH 7.5, 4

(13) Inhibition of SHP-1 activity by PKC-θ regulates NK cell activation threshold and cytotoxicityeLifeMarch 8, 2022Ben-Shmuel A, Sabag B, Puthenveetil A, Biber G, Levy M, Jubany T, Awwad F, Roy RK, Joseph N, Matalon O, et al.drug",Fluo-3-AM,Biotium,50016,1 µg per sample "Chemical compound, drug",p-Nitophenyl phosphate (pNPP),,NEB-P0757S, "Chemical compound, drug",[35S]Met,PerkinElmer,NEG009L005MC, "Chemical compound, drug",Monensin,BioLegend,#420701, Chemical

(14) Aurora Kinase A Is Involved in Controlling the Localization of Aquaporin-2 in Renal Principal CellsInternational journal of molecular sciencesJanuary 11, 2022Baltzer S, Bulatov T, Schmied C, Krämer A, Berger BT, Oder A, Walker-Gray R, Kuschke C, Zühlke K, Eichhorst J, et al.β-mercaptoethanol, 0.01% (w/v) BSA; pH 7.5) containing 10 mM of pNPP substrate (NEB, Frankfurt am Main, Germany; #P0757S) was added to each well and incubated for 30 min at 37 °C. The reaction was stopped by adding NaOH,

(15) Multilayered regulation of autophagy by the Atg1 kinase orchestrates spatial and temporal control of autophagosome formationMolecular CellDecember 16, 2021Anne Schreiber, Ben C. Collins, Colin Davis, Radoslav I. Enchev, Angie Sedra, Rocco D’Antuono, Ruedi Aebersold, Matthias PeterAnalytics,SRP301 d-Desthiobiotin,Sigma,Cat#D1411 dNTPs,Thermo Fisher Scientific,Cat#R0193 L-Glutathione reduced,Sigma,Cat#G4251 p-Nitrophenyl Phosphate,NEB,P0757 L-a-Phosphatidylethanolamine,Avanti,Cat#840026 L-a-Phosphatidylinositol,Avanti,Cat#840042 L-a-Phosphatidylcholine,Avanti,Cat#840055 L-a-Phosphatidylserine,Avanti,Cat#840032 PageRuler

(16) Phase Separation of Disease-Associated SHP2 Mutants Underlies MAPK HyperactivationCellOctober 1, 2020Guangya Zhu et al.peptide,QiangYao,N/A "6,8-difluoro-4-methylumbelliferyl phosphate(DiFMUP)",Invitrogen,Cat# D22065 p-Nitrophenyl Phosphate(PNPP),NEB,Cat# P0757S Doxycycline hyclate,Yeasen,Cat# 60204ES08 G418 Sulfate,Yeasen,Cat# 60220ES03 Puromycin Dihydrochloride,ThermoFisher Scientific

(17) Analysis of polyphenolic metabolites from in vitro gastrointestinal digested soft fruit extracts identify malvidin-3-glucoside as an inhibitor of PTP1BBiochemical PharmacologyAugust 1, 2020Sisir Kumar Barik, Budheswar Dehury, Wendy R. Russell, Kim M. Moar, Morven Cruickshank, Lorraine Scobbie, Nigel Hoggardpurchased from Sigma Aldrich, Germany, and the substrate pNPP (p-Nitrophenyl phosphate: Product No. P0757S) was purchased from New England Biolabs, UK. Malvidin-3-glucoside (0911S), cyanidin-3glucoside (0915S)

(18) The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesioneLifeMarch 29, 2019Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ.kDa-FITC-dextran Sigma Cat#FD250S-100MG Chemical compound, drug Para-Nitrophenolphosphate (pNPP) New England Biolabs Cat#P0757 Chemical compound, drug IPTG Generon Cat#GEN-S-02122 Chemical compound, drug D-biotin Sigma Cat#B4639 Chemical compound,

(19) Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathwayElifeJanuary 3, 2019Brittany K Miller, Ryan Hughes, Lauren S Ligon, Nathan W Rigel, Seidu Malik, Brandon R Anjuwon-Foster, James C Sacchettini, Miriam BraunsteinIgG,Bio-Rad,Cat. #: 1706515,"(1:25,000)" "Chemical compound, drug",p-Nitrophenyl Phosphate (PNPP),NEB,Cat. #: P0757, "Software, algorithm",ImageJ,https://imagej.nih.gov/ij/,RRID:SCR_003070, "Software, algorithm",Graphpad

(20) Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathwayeLifeJanuary 3, 2019Brittany K Miller, Ryan Hughes, Lauren S Ligon, Nathan W Rigel, Seidu Malik, Brandon R Anjuwon-Foster, James C Sacchettini, Miriam BraunsteinIgG,Bio-Rad,Cat. #: 1706515,"(1:25,000)" "Chemical compound, drug",p-Nitrophenyl Phosphate (PNPP),NEB,Cat. #: P0757, "Software, algorithm",ImageJ,https://imagej.nih.gov/ij/,RRID:SCR_003070, "Software, algorithm",Graphpad

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(1)

10/26/2018Assess Migration of Cells Using p-Nitrophenyl Phosphate (PNPP) Sadaf AtarodThe aim of this experiment was to determine the migratory capacity of cells by detecting the protein phosphatase activity. The reagent was recommended by colleagues.