Monarch Plasmid Miniprep Kit from New England Biolabs

Product Specs
ItemMonarch Plasmid Miniprep Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberT1010S
Quantity50 preps
ApplicationsDNA Manipulation
YieldUp to 20 µg. Yield depends on plasmid copy number, host strain, culture volume, and growth conditions.
SourceBacterial
Nucleic Acid TypePlasmid DNA
Temperature25 C
Brand/TrademarkNew England Biolabs Inc (NEB)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Expression conditions and characterization of a novelly constructed lipoprotein intended as a vaccine to prevent human Haemophilus influenzae infectionsJournal of Biological ChemistryAugust 1, 2023Ravinder Kaur, Jill Mangiafesto, Karin Pryharski, Sailee Rasam, Robert Zagursky, Michael Pichicherowas isolated from E. coli/pACYC184 (ATCC #37033) using the Monarch plasmid miniprep kit (catalog # T1010S, New England Biological (NEB)) and digested with EcoR1 and Sca1 (NEB). The lnt gene was isolated from

(2) Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing ConditionsNanomaterials (Basel)May 31, 2023David Arango, Javier Cifuentes, Paola Ruiz Puentes, Tatiana Beltran, Amaury Bittar, Camila Ocasión, Carolina Muñoz-Camargo, Natasha I. Bloch, Luis H. Reyes, Juan C. CruzMastermix (NEB-M0515), Monarch PCR & DNA Cleanup Kit (NEB-T1030S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Bio labs (Ipswich, MA, USA). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer

(3) Polyvalent guide RNAs for CRISPR antiviralsiScienceNovember 18, 2022Rammyani Bagchi, Rachel Tinker-Kulberg, Mohammad Salehin, Tinku Supakar, Sydney Chamberlain, Ayalew Ligaba-Osena, Eric A. JosephsUniversal Human Reference RNA,Invitrogen,Cat#QS0639 Human Lung Total RNA,Invitrogen,Cat#AM7968 Monarch Plasmid Miniprep Kit,New England Biolabs,Cat#T1010S Taq 2X Master Mix,New England Biolabs,Cat#M0270L RNeasy Plant Mini Kit,Qiagen,Cat#74904 DNAse I,Ambion,Cat#AM2222 iTaq

(4) Rapid modeling of experimental molecular kinetics with simple electronic circuits instead of with complex differential equationsFrontiers in Bioengineering and BiotechnologySeptember 28, 2022Yijie Deng, Douglas Raymond Beahm, Xinping Ran, Tanner G. Riley, Rahul Sarpeshkar(Salis, 2011). The plasmid DA313 was purified by Monarch plasmid miniprep kit (New England BioLabs, cat# T1010S) and its concentration was determined by a fluorometric assay with a DNA-specific dye EvaGreen (Ihrig

(5) Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensorsiScienceSeptember 16, 2022Sandra Burgstaller et al.Roth,Cat#6781 Lysozym,AppliChem,A4972 MgCl2 6H2O,Carl Roth,Cat#A537 Monarch Plasmid Miniprep Kit,New England Biolabs,Cat#T1010S NaCl,Carl Roth,Cat#9265 Neurbasal medium,Thermo Fisher Scientific,Cat#21103049 N-Methyl-D-Glucamine

(6) Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAsBasic research in cardiologyJune 23, 2022Kokot KE, Kneuer JM, John D, Rebs S, Möbius-Winkler MN, Erbe S, Müller M, Andritschke M, Gaul S, Sheikh BN, et al.InFusion HD Cloning Kit (102581, Takara, US). Plasmids were purified with Monarch Plasmid Miniprep Kit (T1010S, NEB, US) and the insert was validated with Sanger sequencing. Primers are listed in Supplementary Table

(7) An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell linesSTAR ProtocolsJune 17, 2022Bibekananda Kar, Ankit Sabharwal, Santiago Restrepo-Castillo, Brandon W. Simone, Karl J. Clark, Stephen C. Ekker(Cat#12163).Alternatives: Other plasmid extraction kits, such Monarch Plasmid Miniprep Kit (NEB Cat. No. T1010S) can also be used.g.Set up the following diagnostic digest reaction for verification of the isolated

(8) Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulationSTAR ProtocolsJune 1, 2022Daniel Larcombe-Young, Lynsey Whilding, David Marc Davies, Benjamin Draper, Natasha Bechman, John MaherMonarch DNA Gel extraction kit,New England Biolabs,Cat# T1020S Monarch Plasmid Miniprep kit,New England Biolabs,Cat# T1010S Quick DNA Ligation kit,New England Biolabs,Cat# M2200S ,, Experimental models: Cell lines,Experimental models

(9) CD4+ effector T cells accelerate Alzheimer's disease in miceJournal of neuroinflammationNovember 19, 2021Machhi J, Yeapuri P, Lu Y, Foster E, Chikhale R, Herskovitz J, Namminga KL, Olson KE, Abdelmoaty MM, Gao J, et al.LB agar media followed by DNA isolation and clean-up using monarch plasmid miniprep kit (Catalog no. T1010S, New England BioLabs) and samples were submitted for Sanger sequencing with M13 primers at the University

(10) Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reportersNature ProtocolsSeptember 1, 2021C. G. Piett, T. J. Pecen, D. J. Laverty, Z. D. Nagelefficiency DH5α competent cells (Invitrogen, cat. no. 18258012) Monarch Plasmid Miniprep Kit (NEB, cat. no. T1010S) Nalgene PPCO centrifuge bottles (Thermo Scientific, cat. no. 3120-9500PK) Nuclease-free water diethylpyrocarbonate

(11) Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic LibrariesmSystemsJune 29, 2021Terence S. Crofts, Alexander G. McFarland, Erica M. Hartmannsubsequently used as input for plasmid purification with a miniprep kit (New England Biolabs, catalog no. T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified,

(12) A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioningApplied Microbiology and BiotechnologyNovember 1, 2020Andreas Schüller, Lisa Wolansky, Harald Berger, Lena Studt, Agnieszka Gacek-Matthews, Michael Sulyok, Joseph Strausspurified plasmids (New England Biolabs; Ipswich, MA, USA; Monarch® Plasmid Miniprep Kit; Art. No.: T1010S) were verified by sequencing (“Ready2Run” by LGC Genomics GmbH; Berlin, Germany). All plasmids that

(13) A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking MutagenesisBIO-PROTOCOLAugust 5, 2020Paul J Steiner, Zachary T Baumer, Timothy A WhiteheadT1030S/L) 11. Miniprep kit (e.g., Monarch® Plasmid Miniprep Kit, New England BioLabs, catalog number: T1010S) 12. T4 Polynucleotide Kinase (New England BioLabs, catalog number: M0201S/L) 13. Nt.BbvCI (New England BioLabs

(14) Structure and Function of a Bacterial Gap Junction AnalogCellJuly 11, 2019Gregor L. Weiss, Ann-Katrin Kieninger, Iris Maldener, Karl Forchhammer, Martin PilhoferAssays,Critical Commercial Assays,Critical Commercial Assays ,, Monarch Plasmid Miniprep Kit,NEB,Cat#T1010S Monarch DNA Gel Extraction Kit,NEB,Cat#T1020S Monarch PCR & DNA Cleanup Kit,NEB,Cat#T1030S ATP Determination

Write a Review

(1)

8/17/2022My Favorite Mini-Prep Kit Aditi KothariI love this kit a lot. I have used a lot of plasmid miniprep kits and this one is great because it is the easiest, fastest and can be used with small amount of cells.
(1)

4/9/2020Very Good Miniprep Kit Xuewei WangRecommended for plasmid extraction.