phi29 DNA Polymerase from New England Biolabs

Product Specs
Itemphi29 DNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0269L
Concentration10,000 units/ml
Quantity1250 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 0.5 pmol of dCMP to an acid-insoluble material in a total reaction volume of 50 µl in 10 minutes at 30°C in 50 mM Tris-HCl (pH 7.5), 10 mM MgCl₂ with 4 mM dithiothreitol, 0.2 mg/ml BSA, 0.01 mg/ml HindIII-digested ? DNA, 0.2 µM dCTP including ³²P dCTP, 0.2 mM dGTP, 0.2 mM dATP and 0.2 mM TTP.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Microhomology-mediated circular DNA formation from oligonucleosomal fragments during spermatogenesiseLifeOctober 17, 2023Jun Hu, Zhe Zhang, Sai Xiao, Yalei Cao, Yinghong Chen, Jiaming Weng, Hui Jiang, Wei Li, Jia-Yu Chen, Chao Liualcohol once, followed by ethanol precipitation. EccDNAs were then amplified by phi29 polymerase (NEB, M0269L) at 30°C for 16 hr. Phi29-amplified DNA was cleaned by phenol:chloroform:isoamyl alcohol once, followed

(2) Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesisNatureAugust 1, 2023Yang F, Su W, Chung OW, Tracy L, Wang L, Ramsden DA, Zhang ZZZ.circular DNA, 5 μl 10x Phi29 DNA Polymerase buffer, 1 μl Phi29 DNA Polymerase (New England Biolabs, Cat # M0269L), 2.5 μl 10 mM dNTP (Qiagen, Cat # 201901), 2.5 μl Exo Resistant Random Primer (Thermo Scientific, Cat

(3) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.dNTP, polymerase 1 µl, nuclease-free water up to 100 µl) was performed with phi29 DNA polymerase (NEB, M0269L) at 30 °C for 20 min, followed by 0.75× AMPure XP cleanup; (5) KAPA HiFi HotStart ReadyMix (Roche, 07958935001)

(4) Single-cell genomics for resolution of conserved bacterial genes and mobile genetic elements of the human intestinal microbiota using flow cytometryGut MicrobesDecember 31, 2022Dylan Lawrence, Danielle E. Campbell, Lawrence A. Schriefer, Rachel Rodgers, Forrest C. Walker, Marissa Turkin, Lindsay Droit, Miles Parkes, Scott A. Handley, Megan T. BaldridgeB0269S), 1uL of Exo-resistant random hexamers (MCLAB ERRP-110), 0.5uL of Phi29 DNA polymerase (NEB M0269L), and 0.40uL of NEB BSA (B9000S, resuspended to 10 mg/mL) per well were combined and incubated at 30°C

(5) A Phi29-based unbiased exponential amplification and genotyping approach improves pathogen detection in tick samplesFront Vet SciNovember 7, 2022Xi Zhang, Jingjing Chen, Pengfei Jiang, Heling Xu, Qi Zhang, Huan Zhang, Xiaohu Han, Zeliang Chenreaction time was set for 8 h, 16 h, and 24 h. The amplification was performed using the phi29 DNA Polymerase (#M0269L, NEB, USA)according to the manufacturer's instructions. A master mix was prepared by adding 2.5 μM random

(6) TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switchMolecular cellNovember 3, 2022Yadav T, Zhang JM, Ouyang J, Leung W, Simoneau A, Zou L.Fischer,C10337 Formamide,Sigma,47671-1L-F 20x SSC,Invitrogen,AM9763 Azide-PEG3-Biotin,Sigma,762024-10MG phi29 DNA Polymerase,NEB,M0269L dNTP Set (100 mM),Thermo Fischer,10297018 PureLink Genomic DNA Mini Kit,Thermo Fischer,K182002 ULTRAhyb

(7) High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITSISME CommunicationsOctober 10, 2022Douglas B. Rusch, Jie Huang, Chris Hemmerich, Matthew W. Hahnprimer (10 μM), 37 μL ultrapure water, 5 μL of 10× Phi29 Buffer, and 1 μL of Phi29 (Catalog number: M0269L Vendor: New England Biolabs Inc). The combined products were bead cleaned at a 0.5× bead ratio and eluted

(8) Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolyticamSystemsAugust 30, 2022Caleb Walker, Seunghyun Ryu, Sergio Garcia, David Dooley, Brian Mendoza, Cong T. Trinhplasmids were amplified using random hexamer primers (Thermo Scientific SO181) and ϕ29 DNA polymerase (NEB M0269L), as previously described (47), with the exception of using the commercial ϕ29 reaction buffer from

(9) Single-stranded DNA aptamer-based rolling circle amplification as anti-chicken Salmonella bacteriostaticVeterinary WorldMay 11, 2022Samer Sadeq Hameed, Adil Sabr Al-Ogaili, Noor Nooriat 4°C as recommended by the manufacturer. Isothermal ɸ29 DNA polymerase with master mix (NEB, Cat# M0269L MA, USA) was used as the polymerization enzyme. The polymerization incubation condition was 30°C for

(10) Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapyNature Biomedical EngineeringMarch 28, 2022Chen Sun, Leqian Liu, Liliana Pérez, Xiangpeng Li, Yifan Liu, Peng Xu, Eli A. Boritz, James I. Mullins, Adam R. Abatedroplets The MDA reaction mixture was prepared with phi29 DNA Polymerase (New England BioLabs catalog no. M0269L) following the manufacturer’s protocol. Purified T cell DNA was added into a 100 μl reaction containing

(11) Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodiesMolecular cellMarch 4, 2021Zhang JM, Genois MM, Ouyang J, Lan L, Zou L.Fischer,10297018 Formamide,Sigma,47671-1L-F Ni-NTA beads,Invitrogen,R90115 phi29 DNA Polymerase,NEB,M0269L PureLink™ Genomic DNA Mini Kit,Thermo Fischer,K182002 TelC-FITC,PNA Bio,F1009 Experimental Models:

(12) Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of TelomeresCell reportsAugust 4, 2020Segura-Bayona S, Villamor-Payà M, Attolini CS, Koenig LM, Sanchiz-Calvo M, Boulton SJ, Stracker TH.,Thermo Fisher Scientific,Cat# P36935 Crystal Violet,Sigma-Aldrich,Cat# HT90132 phi29 DNA Polymerase,NEB,Cat# M0269L phi29 DNA Polymerase,Thermo Fisher Scientific,Cat# EP0092 "ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml",Perkin Elmer

(13) Regulation of Mother-to-Offspring Transmission of mtDNA HeteroplasmyCell metabolismDecember 3, 2019Latorre-Pellicer A, Lechuga-Vieco AV, Johnston IG, Hämäläinen RH, Pellico J, Justo-Méndez R, Fernández-Toro JM, Clavería C, Guaras A, Sierra R, et al.and dehydrated in ethanol. RCA was performed using 1U/ μL of Φ29 DNA polymerase (New England Biolabs M0269L) for 1 hour at 37°C in a shaking platform. Slides were washed three times with Buffer A and Lin16 and

(14) Cell-type-specific dysregulation of RNA alternative splicing in short tandem repeat mouse knockin models of myotonic dystrophyGenes & DevelopmentDecember 1, 2019Curtis A. Nutter, Jodi L. Bubenik, Ruan Oliveira, Franjo Ivankovic, Åukasz J. Sznajder, Benjamin M. Kidd, Belinda S. Pinto, Brittney A. Otero, Helmut A. Carter, Eric A. Vitriol, Eric T. Wang, Maurice S. Swanson

(15) Nasal Resistome Development in Infants With Cystic Fibrosis in the First Year of LifeFrontiers in MicrobiologyFebruary 26, 2019Aurélie Allemann et al.multiply-primed rolling circle amplification approach (Dean et al., 2001): 10 U of φ29 polymerase (NEB, cat. No. M0269L) were used to amplify 5 μL of extracted DNA with a final concentration of 50 μM random primers (Promega,

(16) Alternative Lengthening of Telomeres through Two Distinct Break-Induced Replication PathwaysCell ReportsJanuary 22, 2019Jia-Min Zhang, Tribhuwan Yadav, Jian Ouyang, Li Lan, Lee Zousodium,Sigma,D8906–10G Formamide,Sigma,47671–1L-F 20× SSC,Thermo Fischer,AM9763 Azide-PEG3-Biotin,Sigma,762024–10MG phi29 DNA Polymerase,NEB,M0269L dNTP Set(100 mM),Thermo Fischer,10297018 PureLink Genomic DNA Mini Kit,Thermo Fischer,K182002 Sequence-Based

(17) Single-cell DNA sequencing reveals a late-dissemination model in metastatic colorectal cancerGenome ResearchAugust 1, 2017Marco L. Leung, Alexander Davis, Ruli Gao, Anna Casasent, Yong Wang, Emi Sei, Eduardo Vilar, Dipen Maru, Scott Kopetz, Nicholas E. Navinwas added into each well, and the plate was centrifuged. MDA was performed using Φ29 polymerase (NEB, M0269L) with 1 mM hexamers (with phosphorothioate modification at the last 2 bases) and 1 mM dNTP (NEB, N0446S).

(18) Highly multiplexed targeted DNA sequencing from single nucleiNature ProtocolsFebruary 1, 2016Marco L Leung, Yong Wang, Charissa Kim, Ruli Gao, Jerry Jiang, Emi Sei, Nicholas E Navinapprovals are required from users’ institutions. Φ29 Polymerase (10 units per 1 μl; NEB, cat. no. M0269L; Polymerase buffer is included) dNTP set, 100 mM (NEB, cat. no. N0446S) PBS 1× Qubit dsDNA high-sensitivity

(19) Structural insights into the DNA-binding specificity of E2F family transcription factorsNature CommunicationsDecember 3, 2015Ekaterina Morgunova, Yimeng Yin, Arttu Jolma, Kashyap Dave, Bernhard Schmierer, Alexander Popov, Nadejda Eremina, Lennart Nilsson, Jussi TaipaleNew England BioLabs, M0202L), (e) fill-in reaction with phi29 DNA polymerase (10 U, New England BioLabs, M0269L), (f) lambda exonuclease reaction (10 U, New England BioLabs, M0262L) and (f) RecJf exonuclease reaction

(20) SNES: single nucleus exome sequencingGenome BiologyDecember 1, 2015Marco L Leung, Yong Wang, Jill Waters, Nicholas E Navinperformed on single flow-sorted nuclei using 10 units of Φ29 polymerase and 10× Φ29 buffer (NEB cat#M0269L), 1 mM dNTP (GE Healthcare, cat#28-4065-51), and 50 μM random hexamer (phosphorothioate modification

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Veterinary World

phi29 DNA Polymerase from New England Biolabs

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New England Biolabs

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phi29 DNA Polymerase from New England Biolabs

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