Ph.D.-12 Phage Display Peptide Library from New England Biolabs

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New England Biolabs for
Ph.D.-12 Phage Display Peptide Library

Description

The Ph.D.™-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1-6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 2.7 x 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.